lessons from gene targeting experiments in the animal model of eae
Báo cáo khoa học: " Radiation-induced skin injury in the animal model of scleroderma: implications for post-radiotherapy fibrosis" ppt
Ngày tải lên :
09/08/2014, 09:22
... leads to the production and secretion of a larger mutant FBN-1 protein [14] The mutant protein expresses an increase in the number of TGF-β binding motifs resulting in more efficient binding of TGF-β ... of radiation in case of scleroderma Further studies are required to elucidate the role of FBN-1 in controlling TGF-β1 signaling in TSK mouse The underlying mechanism of radiation resistance in ... degrees of skin damage initially, the injury in TSK mice healed promptly and ultimately exhibited signs of less fibrosis This study is the first report on the effects of radiation in an animal model...
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Báo cáo y học: "Regional assessment of articular cartilage gene expression and small proteoglycan metabolism in an animal model of osteoarthritis" pptx
Ngày tải lên :
09/08/2014, 06:23
... occur in the articular cartilage in the ovine lateral meniscectomy model of OA [22,23,33] The present study extends these earlier investigations by examining the expression of a number of important ... intact core protein (43 kDa) and a number of fragments (39, 32, 28 and 26 kDa) in all of the specimens There was an increase in staining intensity for all biglycan core protein species in menisR856 ... elevation in synthesis [17,18] Degradation of the ECM is also elevated in these early stages in OA Eventually, the biosynthetic machinery of the chondrocyte is unable to keep up with the anabolic...
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Báo cáo y học: "The laminin b1-competing peptide YIGSR induces a hypercontractile, hypoproliferative airway smooth muscle phenotype in an animal model of allergic asthma" potx
Ngày tải lên :
12/08/2014, 13:22
... determined Therefore, using a guinea pig model of chronic asthma, we explored the role of laminins in ASM remodelling in vivo, by treating the animals with the specific soluble laminin-competing peptide ... contains the RGD-binding motif present in ECM proteins like fibronectin, collagens and laminins [24,25] The specific role of laminins in ASM remodelling in vivo, however, remains to be determined ... [17] In adult asthmatics, expression of laminin a2 and b2 chains in the airways is increased [18,19] In addition, asthmatics with compromised epithelial integrity show increased laminin g2 chain...
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Báo cáo y học: "Renal hypoperfusion and impaired endothelium-dependent vasodilation in an animal model of VILI: the role of the peroxynitrite-PARP pathwa" ppt
Ngày tải lên :
13/08/2014, 20:21
... similar in all animals until the 120th minute of MV when the PaO2/FiO2 started decreasing in the high Vt+Vehicle group compared with the other groups (Figure 2a) There were no differences in the ... staining: wheat germ agglutinin; blue staining: nuclei; green staining: (c) CD45, (d) NGAL These observations may warrant a discussion of potential cause-effect relations in a complex model of inter-organ ... above Thereafter ventilation setting was changed according to the randomization and continued for 3.5 hours PJ-34 was administered iv as a continuous infusion at mg/kg/h for the remainder of the experiments...
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báo cáo hóa học: " Activation of retinal microglia rather than microglial cell density correlates with retinal neovascularization in the mouse model of oxygen-induced retinopathy" ppt
Ngày tải lên :
19/06/2014, 22:20
... directly involved in the formation of retinal NV in the later stages of the OIR model In this study, we investigated the temporal and spatial distribution of microglial cells during the different ... animal care guidelines of the Institute for Laboratory Animal Research (Guide for the Care and Use of Laboratory Animals) in accordance with the ARVO Statement for the “Use of Animals in Ophthalmic ... positive lectin stain For each retina, the mean number of microglial cells Page of from the three fields of each counting position (central or peripheral zone, and layer) was calculated Then, the mean...
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Multi-tissue gene-expression analysis in a mouse model of thyroid hormone resistance docx
Ngày tải lên :
09/08/2014, 20:20
... cell-cycle/growth-inhibitor genes and WAT In RTH mice this suggests suppression of lipogenesis in the WAT with simultaneous increase in lipogenesis in the heart ing T3 These findings raise the possibility that induction ... with half of the genes being induced in the heart of TRβPV/PV mice (blue bar, Figure 3) These patterns are consistent with the negative regulation of lipogenesis in the hyperthyroid heart interactions ... a second set of wild-type animals selected in the same way (the control group) In the RTH experiments, we identified changes in gene expression resulting from the in vivo action of PV and/or...
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Báo cáo y học: "Topical anti-inflammatory activity of Polygonum cuspidatum extract in the TPA model of mouse ear inflammation" docx
Ngày tải lên :
11/08/2014, 08:21
... weight of PCE Most of the solids in the commercial extract were not soluble in 50% ethanol The dry weight of the ethanol-insoluble pellet remaining after centrifugation of the extract from 1.0 g of ... was induced in both ears of each mouse by the topical application of µg TPA dissolved in 20 µL of acetone to both the inner and outer ear surfaces Thirty minutes after the application of TPA, the ... All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Georgia and conducted according to IACUC guidelines The sample size of animals...
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Báo cáo y học: "Use of a highly sensitive strand-specific quantitative PCR to identify abortive replication in the mouse model of respiratory syncytial virus disease" pptx
Ngày tải lên :
12/08/2014, 01:22
... triplicate in immunofluorescence assay [20] For experiments conducted to investigate inhibition of RSV replication, one group of animals were administered a single intramuscular injection of palivizumab ... cycling (Table 1) The use of this tag is designed to reduce detection of cDNAs synthesised due to RNA self-priming in the reverse RSV infection in vitro To investigate whether RSV RNA synthesis ... for the synthesis of more negative sense genome [11-13] The use of in vivo models with good clinical translation is vital in the search for new treatments for disease A number of different animal...
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Báo cáo y học: " Pharmacological inhibition of leukotrienes in an animal model of bleomycin-induced acute lung injury" doc
Ngày tải lên :
12/08/2014, 16:20
... staining for IL-1 (A) was localized mainly in inflammatory cells and in vascular endothelium There was a marked reduction in the IL-1 immunostaining in the lungs of MK-571 group (B) and in the ... has been recently dissected in the bleomycin animal model of lung fibrosis using different genetic backgrounds Knocking out each of the enzymes involved in the cascade from membrane phospholipids ... lungs of Zileuton group (C) TNF-α was localized mainly in inflammatory cells and in bronchial epithelium of lungs in the bleomycin group (E) A marked reduction in TNF-α immunostaining in lungs of...
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Báo cáo y học: "Evaluation of long-term antinociceptive properties of stabilized hyaluronic acid preparation (NASHA) in an animal model of repetitive joint pain" doc
Ngày tải lên :
12/08/2014, 17:22
... long-term antinociceptive effects of HA preparations, namely the repetitive induction of short-lasting pain states in the joint by the injection of bradykinin, combined with prostaglandin E2 (PGE2) ... the rather weak effect of this substance in the model used Pointing in the same direction, in recordings from nerve fibers of joints, sodium hyaluronate did not reduce the discharges of the nerve ... a cut-off value of 250 g was defined Pain-related guarding behavior of the inflamed hindpaw was assessed by quantification of weight bearing towards the non-inflamed hindlimb using an incapacitance...
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Báo cáo y học: "Elevated calcitonin precursor levels are related to mortality in an animal model of sepsis" pps
Ngày tải lên :
12/08/2014, 18:20
... is a single chain, 32-amino acid peptide that originates from the CALC-I gene on chromosome 11 [16] In humans the highest concentration of tissue iCT is in the parafollicular cells of the thyroid ... incised in the midline Bacterial sepsis was induced by implanting one pellet in the right lower quadrant of the peritoneal cavity of each animal The abdominal incisions were then closed with non-absorbable ... Additionally, insights into the biosynthesis, regulation, and physiologic activity of such a marker may illuminate some of the causative factors in the pathophysiologic and clinical events of the sepsis...
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Báo cáo y học: "Hepatic oxidative stress in an animal model of sleep apnoea: effects of different duration of exposure" potx
Ngày tải lên :
13/08/2014, 13:20
... by the presence in the histological examination of ballooning, steatosis, necrosis and the presence of neutrophils in the liver, similar to what is observed in NASH [45] In the evaluation of ... inhibited by increasing amounts of SOD in the homogenate, was monitored spectrophotometrically at 480 nm The amount of enzyme that inhibited 50% of epinephrine auto-oxidation was defined as U of SOD ... significant increase of nitric oxide in animals exposed to IH simulating OSA (6 min/6 min) during 120 days [48], and to hypobaric hypoxia during 32 days [60] The increase of NO, along with increased...
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Báo cáo y học: "Protective effect of the DNA vaccine encoding the major house dust mite allergens on allergic inflammation in the murine model of house dust mite allergy" ppsx
Ngày tải lên :
13/08/2014, 13:22
... [35] We injected mixed naked DNA into the muscle of the murine model of allergic disorder Some of the injected DNA might be postulated to stay in the nuclei of cells or be integrated in the host ... in a perivascular and peribronchial distribution originating in the center of the lung In grade 3, the perivascular and peribronchial infiltrates extended to the periphery of the lung Measuring ... specific IgE in the vaccinated group was detected than in the control group, suggesting that the gene vaccination might successfully redirect the immune response from Th2 into Th1 to the encoded...
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Gene expression profiling in a rat model of neurodegeneration
Ngày tải lên :
07/10/2015, 09:55
... and neurofibrillary tangles are often present in the affected regions of the brain (Tiraboschi et al 2004) PD arises from the loss of dopamine-generating cells in the substantia nigra and the most ... suggesting neuroprotection These findings not only provide molecular insight into the contribution of chemokines and their receptors in excitotoxic injury involved in stroke and neurodegenerative ... Pentraxin related gene Vimentin Oxidized low density lipoprotein (lectin-like) receptor Integrin, alpha Receptor-interacting serine-threonine kinase Cardiotrophin-like cytokine factor Epithelial...
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Báo cáo y học: "Impact of the introduction of new vaccines and vaccine wastage rate on the cost-effectiveness of routine EPI: lessons from a descriptive study in a Cameroonian health district" potx
Ngày tải lên :
13/08/2014, 11:22
... This involves the introduction of new vaccines and combinations which are generally more expensive In this light, the Pentavalent vaccine, DPT-HB-Hib was introduced (in place of DPT) into the ... assess the impact of the introduction of 2-dose lyophilized DPT-HB-Hib and of vaccine wastage rate on the cost-effectiveness of EPI as well as to identify main explaining factors of such results The ... http://www.resource-allocation.com/content/9/1/9 Page of Figure The Map of Cameroon showing the ten regions coverage and efficiency This is because the quantity of vaccines is determined by the central level on the basis of administrative...
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Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
Ngày tải lên :
07/03/2014, 00:20
... shown in Fig 1B, the distance between the two termini of the inserted Ca2+-binding sites in the model structure of the EF-hand of STIM1 is within ˚ 15 A Accordingly, a total of six glycine linkers ... Ca2+-coordinating loop positions and was introduced to perturb the Ca2+-binding ability of the grafted EF-hand of STIM1 (B) Modelled structure of the engineered protein with the grafted EF-hand Ca2+-binding ... its own structural integrity [15,17] In Fig 1B, the modelled structure of the engineered protein CD2.STIM1.EF is shown The structural integrity of the host protein was then examined by two-dimensional...
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Báo cáo khoa học: The role of the Fe-S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii potx
Ngày tải lên :
15/03/2014, 09:20
... rearrangement of the interdomain interaction affects the electronic condition of the Fe-S cluster through the protein scaffold, resulting in an alteration of the signal rhombicity of the EPR spectrum The ... to NifA; the binding of the ATP analog induces the rearrangement of the GAF and possible AAA+ (the central domain) domains in VnfA Indeed, the limited protease assays confirmed that the conformational ... is the purification of an AnfA variant reported by Austin et al [22] In their study, the N-terminal domain of AnfA was truncated to prevent the intrinsic aggregation of the intact form during...
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Báo cáo khoa học: Identification and characterization of important residues in the catalytic mechanism of CMP-Neu5Ac synthetase from Neisseria meningitidis potx
Ngày tải lên :
22/03/2014, 21:21
... catalytic residues, indicating a role in binding the nucleotide into the active site [15,16] The crystal structure of CNS from Neisseria meningitidis crystallized in the presence of the substrate analogue ... concentrations in the presence or absence of 0.2 mm dithiothreitol Effect of Mg2+ concentration The effect of an increase in the concentration of Mg2+ was determined by measuring the rate of the reaction in ... Neu5Ac from other sugars, or that they are residues proposed to form the binding site of the catalytic Mg2+ ion We have determined the kinetic parameters of the wild-type enzyme from N meningitidis...
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Interactive Food and Beverage Marketing: Targeting Adolescents in the Digital Age pdf
Ngày tải lên :
29/03/2014, 20:20
... techniques in the advertising industry; others are unique to the interactive environment Taken together—and viewed against the backdrop of the major changes taking place in the worlds of media, ... part from the proprietary nature of the research within the marketing industry itself) [9,10] There have been only a few studies of online food marketing practices in the years since the report ... manipulative marketing techniques Finally, given the increasing interest in addressing the role food marketing plays in the global youth obesity crisis, there is a need for a coordinated international...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
Ngày tải lên :
18/06/2014, 15:20
... [21,43], if the function of the miRNA is dominant in leading the mRNA target degradation, however, most animal miRNAs pair to the 3' UTRs of their targets by incomplete base-pairing through their seed ... analysis of these genes also divided the samples into three groups, hES, EB, and adult cells, and divided the genes into major nodes (Figure 4) The node containing 226 genes that were upregulated in ... contained the genes that were upregulated in both hES cells and EB, node B contained the genes upregulated in hES cells only, node C contained the genes upregulated in EB only, and node D contained...
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