recql4 gene targeting in mice

... expressed genes and how many of the eQTLs specific for the 77 pathway genes defined above mapped to each interval In general, assuming under the null hypothesis that the genomic location of genes influencing ... with genetically altered mice, we have studied a heterogeneous population where the genetic variations leading to the phenotypes and the changes in gene expression are all naturally occurring In ... originating from an F2 intercross carried out between mouse strains C57BL/6J and DBA/2J This intercross encompassed a two-phase study as described in Colinayo et al [28], using 142 female F2 mice...

Ngày tải lên: 14/08/2014, 14:21

... for gene delivery in the mouse liver In order to achieve gene targeting in vivo using an adenovirus vector or any other means, it will be necessary to increase the frequency of gene targeting ... of euchromatin [38,39] Reducing the copy number of the transgene and/ or using another transgene that is lower in CpG content might increase gene targeting, although the decrease in copy number ... 213 bp 288 bp Figure Design for gene targeting and its detection Design for gene targeting and its detection (A) The donor carrying the mutant lacZ gene is inserted into an adenovirus vector The...

Ngày tải lên: 14/08/2014, 19:22

... protein The naturally occurring fingers with known binding preference were disassembled to find fingers for all 64 different target triplets to enable the generation of zinc finger domains binding ... (ENT) b Zinc finger nucleases (ZFN) are synthetic fusion proteins of a DNA-binding zinc finger domain and a double strand break-inducing nuclease domain Dimerization of nuclease domains is necessary ... sequence blocks being known, there is no general assembly code for the generation of consistently high affinity binding of zinc fingers This makes screening of zinc finger domain libraries necessary...

Ngày tải lên: 26/11/2015, 09:54

... neuronal intranuclear inclusions (NIIs) containing the human transgene-encoded amino terminus of human huntingtin form more slowly throughout the brain tissue in R6/1 compared to R6/2 mice [29,30] ... protein; RAR, retinoic acid nuclear receptor; WHN, winged helix protein; BKLF, basic krueppel-like factor; ZF5, zinc finger domain; MYT1, zinc finger TF involved in primary neurogenesis; E2A proteins, ... affected in both strains of R6 transgenic HD mice It appeared that the amino terminus of mutant huntingtin containing an expanded polyglutamine tract caused the steady-state CB1 mRNA levels in the...

Ngày tải lên: 16/03/2014, 18:20

... dynamics and reassembly in living cells: targeting of an inner nuclear membrane protein in interphase and mitosis J Cell Biol 138, 1193–1206 26 Hoffmann K, Sperling K, Olins AL & Olins DE (2007) The ... cerevisiae) Shc SH2-domain binding protein bromodomain and WD repeat domain containing serum amyloid A cell division cycle homolog A (S pombe) heat shock 105kDa ⁄ 110kDa protein zwilch, kinetochore associated, ... into pBlueScript vector and sequenced Generation of Tm7sf2 knockout mice A Tm7sf2 targeting vector was generated by subcloning into pBluescript vector a 5380 bp KpnI ⁄ XbaI fragment, containing...

Ngày tải lên: 23/03/2014, 06:20

... impairments [p

Ngày tải lên: 12/06/2014, 15:50

... [19] In preclinical studies in mice, CHS 828 could reduce growth of s.c NB without any signs of toxicity [17] In order to investigate this finding in a clinically more relevant setting, we developed ... CHS 828 hampering uptake in the gastrointestinal tract Clinical trials using orally administered CHS 828 were discontinued due to the variation in exposure levels and dose limiting toxicities ... simplicifolia-1 (BS-1) lectin staining (brown); bar = 40 μm [18,19] which was also observed in a previous study in nude mice [17] This inter-individual variability has partly been explained by variations...

Ngày tải lên: 18/06/2014, 15:20

... diluted in phosphatebuffered saline (pH 7) Mice in three of the four groups were intravenously injected in the tail vein with 13, 130, and 260 kBq 131I, respectively, while the mice in the control ... associated with it were included in this categorization Discussion In the present study, the effects of internal low-dose irradiation by 131I were investigated in vivo Using gene expression microarray, ... Gius D: Distinct effects of ionizing radiation on in vivo murine kidney and brain normal tissue gene expression Clin Cancer Res 2006, 12:3823-3830 Uehara Y, Ito Y, Taki K, Nenoi M, Ichinohe K, Nakamura...

Ngày tải lên: 20/06/2014, 21:20

... seven generations and intercrossed The double congenic mice were established by intercrossing seventh generation mice from Nss1 backcrossbreeding and Idd5 breeding, respectively, to generate mice ... replacing missing values and collapsing probes to genes, the gene lists were filtered by minimum standard deviation = 0.7 Finally, the genes in the list 'pre-processing 1' were removed The resulting ... protein 54 (Tmem54), coiled coil domain containing 28B (Ccdc28b) and testis-specific serine kinase (Tssk3) all to be downregulated Furthermore, we found zink finger and BTB domain containing (Zbtb8)...

Ngày tải lên: 09/08/2014, 10:21

... susceptible inbred mouse strains after influenza A infection Microbes Infect 2010, 12:309-18 Song L, Kolar M, Xing E: KELLER: estimating time-varying interactions between genes Bioinformatics ... co-expressed genes (regulatees) at a specific time point On gene level, we expect to find the regulators of a gene, belonging to cluster B, in the gene pool of cluster A Thus, moving forward in our ... Varying Dynamic Bayesian Networks on time series gene expression data of murine C57BL/6J inbred strain after infection with H1N1 (PR8) virus Our reverse engineering approach combines clustering...

Ngày tải lên: 10/08/2014, 09:22

... ultrasonic gene transfection instrument (UTG 1025, Institute of Ultrasound Imaging of Chongqing Medical Sciences, Chongqing, China) was applied on C and D groups for irradiation after the target gene injection, ... 0.05) with other groups in improving the survival time of tumor-bearing mice Discussion Liver cancer gene therapy requires a non-invasive, efficient, targeting and safe gene transfection technology ... phosphokinase in the cell As a chain terminator, it will interfere with DNA synthesis during cell division, leading to tumor cell death [16,17] A large number of studies have shown that suicide gene...

Ngày tải lên: 10/08/2014, 10:20

... the WNV E protein was engineered to comprise a hexahistidine tag and a cysteine-containing linker at its C-terminus (DIII-C) Upon expression in E coli the protein formed insoluble inclusion bodies ... eliciting neutralizing antibody responses [12] In particular a structurally distinct domain of the E protein (DIII) has been proposed as the receptor-binding domain [13] Antibodies recognizing ... virus incorporating the envelope proteins of WNV into the infectious backbone of a yellow fever vaccine strain is currently being developed as a live-attenuated vaccine [9-11] While immunogenic in...

Ngày tải lên: 12/08/2014, 04:20

... FOS, S100 calcium binding protein A9 [S100A9], etc.) other "later" gene interactions were surprising (Figures and 5) Perhaps the most intriguing finding regarding GMA-MS welding fume Page of 18 ... provide unique insight into strain- and welding fume-dependent genetic factors involved in the mouse lung response to welding fume The gene regulation and network interconnectivity reported in this ... carcinogenic metal-containing SS fumes [11] Therefore, it was interesting that these fumes were associated with modification of behavioral gene networks in both A/J and B6 mice This finding highlights...

Ngày tải lên: 12/08/2014, 11:22

... more reliable Using the remaining genes, we examined all predicted interactions for which the target gene has two or more binding sites For each pair of binding sites on a target gene responsive ... overlapping sites Given the large number of potential binding sites in a localized region of 3' UTR, the increased repression of multiple pairs of binding sites can be explained by having more binding ... polyadenylation element binding protein MECP2 62 NM_004992 Methyl cpg binding protein (Rett syndrome) OTUD4 61 NM_199324 OTU domain containing OGT 60 NM_003605 O-linked N-acetylglucosamine (glcnac) transferase...

Ngày tải lên: 14/08/2014, 08:20

... Genetic Vaccines and Therapy 2004, 2:16 Introduction In vivo gene targeting of highly specific cell subsets remains the main challenge for gene therapy of a broad range ... recently, in vivo, we have shown a specific tumor targeting after a single intravenous injection in mice bearing tumour expressing the renal carcinoma – related G250 tumor associated antigen [16] ... and is readily internalised upon binding to its ligand [17] Thus, targeted -gene transfer through CD117 may be achieved in this cell subset We indeed demonstrated an in vivo targeting of hematopoietic...

Ngày tải lên: 14/08/2014, 19:22

... transcriptsin both slow twitch skeletal muscle as well as the heart It binds Ca2+ and prevents actin-myosin interaction in resting muscle Page of (page number not for citation purposes) Genetic Vaccines ... contains cyclic AMP response-element binding protein (CRE-BP – a member of the leucine zipper family of transcription factors) binding sites as well as NFkappaB binding sites The CMV promoter confers ... were assayed using the Galacto-Star chemiluminescence reporter gene assay system (Tropix, Inc., Bedford, MA, USA) Protein concentrations were determined using the Bio-Rad DC protein assay kit (Bio-Rad,...

Ngày tải lên: 14/08/2014, 19:22

... contribute in gaining insights into the pathogenesis of allergy and in defining the potential strategies for therapeutic intervention (Sakai et al., 1999; Garnier et al., 2002) Indeed, much understanding ... efficacy of a DNA vaccine in an animal challenge model was initially demonstrated in the mice immunized with DNA encoding an internal conserved protein of influenza A nucleoprotein (NP) which elicited ... studies indicated that CD4 T lymphocytes were the primary responding cells in these assays These responses were maintained in vaccines for at least a year (longest time point tested), indicating...

Ngày tải lên: 14/09/2015, 14:20

... decrease in the VEGF protein level and a 22% decrease in the c-Myc protein level in the corresponding NS group A 52% decrease in the expression level was noted in the ODC protein expression assay In ... by a 45-minute incubation in peroxidase-labeled streptavidin (from DAKO kit) Finally the sections were stained with diaminobenzidine-H2O2 solution at room temperature The number of stained cells ... therefore not surprising that CY administration resulted in a decrease in the proliferating cell number in both the gastric and duodenal tissues Indeed the decrease in cell proliferation in gastric tissue...

Ngày tải lên: 02/11/2012, 10:19

... of immunoglobulin gene, γIg – the γ-chain of immunoglobulin gene, IFN-ind – the interferone-inducible gene 6-16, IL4R – the interleukin receptor gene, TAP2 – the transport protein gene, hnRNP A1 ... Hodgkin’s disease; capn4: the calpain subunit gene; ND4: the NADH dehydrogenase subunit gene; IL5: the interleukin gene; λIg: the λ-chain of immunoglobulin gene; γIg: the γ-chain of immunoglobulin ... immunoglobulin gene; IFN-ind: the interferon-inducible gene 6-16; IL4R: the interleukin receptor gene; TAP2: the transport protein gene; hnRNP A1: the human ribonucleoprotein А1 gene; SMG-1 :...

Ngày tải lên: 02/11/2012, 11:08

... protein kinase N1 is a transcript variant encoded by PKN Its cytoplasmic gene product is a serine/threonine-protein kinase N1 which is a member of the protein kinase C superfamily (http://amigo.geneontology.org/cgi-bin/amigo/ ... cannot determine the global players involved in such complex phenotypes Integration of the gene expression profiling with specific modulation of gene expression in relevant signalling pathway ... polymer); the ordinates represent the linear ratio of differential gene expression Genes detected and in following investigated for validation A) an induced expression of the PKN1 gene B) a repressed...

Ngày tải lên: 03/11/2012, 11:44