Để khẳng định sự cú mặt của cDNA-NK1 trong cỏc vector biểu hiện, hai vector biờ̉u hiờ ̣n pCDNA3-NK1-11 và peGFP -N1-NK1-5 được giải trình tự m ột chiờ̀u từ đầu 5’ của đoạn chốn NK1 bằng mụ̀i của vector.
Kờ́t quả giải trình tự cDNA-NK1 trong hai hờ ̣ thụ́ng vector biờ̉u hiờ ̣n trờn đờ̀u cho thấy hơn 750 bp phớa đầu 5’ của cDNA-NK1 khụng có bṍt kỳ sự sai khác nào so với trình tự cDNA gụ́c trong vector tỏch dũng pNK1-3. Điờ̀u đó chứng tỏ rằng chỳng tụi đó thành cụng trong việc thiết kế vector bi ểu hiện mang cDNA hoàn chỉnh mó húa cho thụ thể neurokinin-1 ở người Việt Nam.
KẾT LUẬN VÀ KIẾN NGHỊ KẾT LUẬN
Với những kết quả thu được chỳng tụi đưa ra những kết luận sau:
1. Tỏch dũng thành cụng cDNA hoàn chỉnh mó húa cho thụ thể neurokinin – 1 ở phổi người Việt Nam. cDNA-NK1 cú kớch thước 1338 bp trong đú cú 5 nucleotide (ở cỏc vị trớ 184, 333, 370, 641, 996) khụng giống với trỡnh tự cDNA- NK1 trong ngõn hang dữ liệu, chứng tỏ sự đa hỡnh của gen mó cho thụ thể NK1 ở người Việt Nam.
2. Thiết kế thành cụng vector biểu hiện pCDNA3 và pEGFP-N1 mang cDNA mó cho thụ thể NK1 phõn lập từ phổi của người Việt Nam.
KIẾN NGHỊ
Chuyển nhiễm vector biểu hiện pCDNA3 và pEGFP-N1 mang cDNA-NK1 vào hệ thống tế bào động vật để biểu hiện thụ thể neurokinin – 1.
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