- sic kydd dinh tinh iridoid (Hinh 1):
286 Tgp chiDuac lieu, tap 14, so 5/
Bing 5. Kk qui khao sit him lugng phyllanthm vi lignan trong li vi cugng cua DHCD Phyllanthus amarus TT 1 2 3 4 5 K^ hifu mlu Ml M2 M3 M4 M5 K^t q u i djnh lugng li H i m lu-gng % phyllanthin 1,057 1,206 1,133 0,790 1,150 Him lugng % lignan tSng s6 4,1451 4,1918 3,0240 1,7347 3,4359
K^t qua djnh lugng cu^ng Him lugng % phyllanthin 0,055 0,105 0,054 Him lugng % lignan t6ng so 0,2070 0,1885 0,1748
Nhdn xet: Him lugng lignan chiem chii
yiu trong li DHCD.
4. Ket luan
Da xic dinh vin tay sac ky ldp mdng dinh tinh nhdm hoat chit lignan, hgp chat phenolic va flavonoid ciia dugc lieu DHCD trdng v i thu hii tai Trung tim trong v i che biin ciy thudc Vien dugc li$u.
Phuang phip djnh lugng phyllanthin trong dugc lieu DHCD da xiy dung dat yeu ciu cua phuang phip phin tich dinh lugng vdi do lap lai tdt (rsd: 2,26%, n = 6) v i do diing trung binh dat 100,3%; Khoing tuyin tinh
dugc xic dinh 0,1-0,8 \ig (r: 0,99943; LOD
va LOQ dugc xic dinh li 0,017 va 0,058|ag). Phuong phip tiln hinh nhanh, don gian.
dugc ip dung cd hieu qua trong phan tich va kiem nghiem dugc lieu.
Kit qui dinh lugng cho thay, ham lugng phyllanthin trong cic mau dugc lieu DHCD da khio sat dat tu 0,4-0,7%, ham lugng lignan tdng so tinh theo phyllanthin dat tu 0,95 - 2,80%, trong do cic chit phyllantin, chit 2 (hypophyllanthin) va chit 3 chiim tir 25,5 45,5%, 12,8 - 18,8 va 41,6 - 58,6% trong lignan tdng sd.
Cic thanh phin hoat chit thudc nhdm lignan chiim chu yiu trong bd phan la cua ciy DHCD.
Cic kit qua phan tich neu tren lam co sd nghien ciiu tieu chviin hoi dirge lieu diep ha chau
ding (Phyllanthus amarus Schum.et Thonn.).
Tai lieu tham khao
1 Arvind K Tripathietal (2006)"QuantitativeDetenninationof phyllanthin and hypophyllanthin in PM/««to
Species by High performance Thin Layer Chromatography" Phytochem. Anal. 17: 344-397. 2. Aminui Islam et al (2008) Phyto-Pharmacology of Phyllanthus amarus, an overview. 3. Nguyen thugng Dong Do Trung Dam Nguyin Kim Phugng vi nh&ng ngu^i khic (2001) "Nghien cuu tic dung dugc ly cua bgt phyllanthm Tapcht
rHc lieu tap 6 s6 2-^3/2001, tr 75-81. 4. Nguyin Thugng Dong, Pham Thanh Ky , Pham vin Thanh vi nhung
n T ^ Whic r200n "Nghien cuu so bg thinh phin hoi hgc cua hai loii Phyllanthus" Tap chiDuac lieu, tap 6, so
2S/^oXtr 7^-^^^ '-^^ '^^^ ^''''^ ^'' *"^^ '' '^"^ ""''''" ^'"'^ ' ^''''""'"' ''''^ ""'" ''' ''
ky thuit, tip I, tr 596-598.
Tgp chi Dircrc lieu, tap 14, so 6/2009 (Trang 286 - 292)
PHAN TICH MOT S 6 THANH PHAN VA IVHOM HOAT CHAT
TRONG RAU MEO HEFtBA ORTHOSIPHONIS SPIRALIS BANG
PHirONG PHAP SAC KY L d P MONG KET HgfP DO M A T DO( TLC- SCANNING) PHVC v y NGHIEN CUtJ TIEU CHUAN HOA
Nguyin Kim Bich, Nguyin Thj Phuang, Trjnh ThjNga, Vu Thj Lan, Nguyin Minh Nggc Vi$n Dugc lieu
(Nhin bai ngay 5 thing 11 nam 2009) Summary
Determination of Some Active Components in Herba Orthosiphoris spiralis by TLC-Scanning for Standardization
Thin layer chromatography-fingerprint (TLC-FP) identiflcation of flavonoids, terpennoids and caffeic acid derivatives together with the quantitative dethermination of sinensetin and ursolic acid on Herba Orthosiphonis spiralis were performed Solvent systems of dichloromethan : ethylacetat 6: 4 (1), chloroform : ethylacetat 5 : 2 (2) and toluen : ethylacetat: aceton : acid formic 5:2:2:1 (3) were used for separation of the group: flavonoids, terpenoids and sinensetin, respectively. Detection of the flavonoids and caffeic acid derivatives are observed under UV light at 366nm before and after spraying with NP/ PEG reagent. For detection of terpennoids, visualization by spraying with sulfuric acid 10% and heating. For quantitative analysis of sinensetin and ursolic acid , the solvent system (1) was applied Fluorometric scanning at A366nm , K400for quantitation of sinensetin and scanning at X 520 nm for quantitation of ursolic acid after spraying withl0% sulfuric acid solution in ethanol and heat at 110 ' C for 5 minute. The calibration curves linear at 0,05- 0,25 pg (sinensetin) and 0,5- 2,5 pg (ursolic acid), precision (RSD) 2,25 % and 4,88 % (n =6), average recovery 100,95% and 100,07%, respectively. The content of sinensetin and ursolic acid in the herba are estimated 0,017- 0,044% and 0,20 - 0,39% (n = 6), respectively.
Key words: Fluorometric scanning, qualitative, quantitative, sinensetin, ursolic acid, caffeic acid derivatives.