D6i tugng khao sat g6m cac mlu ma de dugc thu d Trung tam trdng va ch^ biSn cay thuoc Vien Dugc Lieu va mgt s6 noi khac d Ha Ngi, dugc kiSm djnh ten khoa hgc tai Khoa Tai Nguyen cay thuoc Vien Dugc Lieu. Cac mlu deu dugc thu d giai doan ra hoa, xir ly d6n kho d nhiet do 40-50° C.
Chuan bj mau
Mdu thu: Chilt lg dugc lieu da tan nho
voi 20ml hSn hgp methanol nudc (4:1), lie sieu am 20 phiit, lam nhic lai 2 lin, gop dich chiet, b6c hoi dung moi tren each thiiy den gan c ^ , ti6p tuc lam kho trong h6t Hoa cin thu dugc trong 25ml methanol, lgc bo 1ml dung dich dau, liy 5ml dung dich tidp theo lam mau thii chim sSc ky.
Mdu doi chieu: Dung dich aucubin 0,1
mg/ml trong methanol.
Dieu kien phan tich
He thdng sic ky ldp mong CAMAG g6m CO may chim kinh CAMAG- linomat 5, May do mat dp densitometry CAMAG TLC SCANNER 3, dugc su dung vod sir ho trg ciia phan mem Wincats. Lop mong TLC plate silicagel 60 F254 Merck, kich thuoc 20 xlO cm sii dung cho sac ky. Chara thang chuan va cac mau thir vdi lugng cham 5 ^il; 10|il x 2 lin; 15}il X 2 lan; 20^1 cua dung dich aucubin va 4
10|il ciia dung dich mau thii, cham nhic lai 2- 3 lan doi vdi moi mau thu. Lop mong dugc trien khai den 80mm vdi he dung moi aceton: xylen : isopropanol: ammoniac dam dac (10: 30: 50: 10), trong binh sac ky da dugc bao hoa 30 phut trudc khi trien khai sac ky. Kinh sau khi lam kho het dung moi, dugc nhugm thudc thii DMAB (hdn hgp ty le 2:1 cua dung dich 4. dimethylaminobenzaldehyd trong ethanol va acid hydrocloric 36%), de yen 15 phiit va tiep tuc lam khd them Ih 45 phiit d trong hdt. Tien hanh do mat do d X max 590nm, tdc do quet 2bmin/s. Lugng aucubin trong mlu thu dugc xac dinh tu ket qua dudng cong chuan da xay dung.