0,2-0,5cm. Mat ngoai mau vang din nau, phii diy long ngin. Phia dudi ong trang cd 5 la dai nhd, mau luc, 5 nhi va 1 vdi nhuy. Mui tham nhe, vi hoi ding. Hoa da nd dai tii 2-5cm, trang chia thanh hai mdi cudn nguge lai. Mdi tren xe thanh 4 thuy, mdi dudi nguyen. Nhi va vdi nhuy thudng thd ra ngoai trang hoa.
4. Dac diem vi hoc:
Dac diem bdt dugc lieu: Bdt mau vang nhat, cd miii tham nhe, vi hai ding. Soi kinh hien vi thay: Nhimg manh canh hoa mang tinh the calxi oxalat hinh cau gai. Manh la bac mang ldng che chd. Ldng che ehd dan bao thanh day, nhan. Ldng tiet. Manh mach dang xoan. Tinh the calxi oxalat hinh cau gai. Hat phan trdn, kich thudc khoang 0,07-0,08mm.
5. Dinh tinh:
* » F
Dinh tinh ddn xuat acid caffeic
Chudn bi mdu:
- Miu thu: 0,5 g dugc lieu cit nhd, chiet sieu am vdi 10 ml methanol, thdi gian
30 phiit, dich chilt thu dugc dung dl chim sac ky. Lugng cham 2 - 4 ^1. - Miu ddi chilu: Acid caffeic 0,5 mg/ml methanol, lugng cham 4^x1.
L&p mong: TLC silica gel 60 F254 Merck, hoat hda kinh d 105 V trong 1 gid trudc
khi sii dung.
He dung moi sdc ky: HDM 1: Ethylacetat: acid fomic : nudc ( 8 : 1 : 1 ) Phdt Men:
Quan sat /UV 366nm -> Hinh 4.11(A).
NP/PEG (TT) quan sat/UV366nm^ Hinh 4.11(B) i^eV^Mfl:Hinh4.11(A,B)
Dinh tinh terpenoid
Chudn bi mdu:
- Miu thu: 0,5 g dugc lieu cit nhd, chilt sieu am vdi 10 ml methanol, thdi gian
30 phiit, dich chilt thu dugc dung dl chim sic ky. Lugng chim 2 - 4 ^1. Miu ddi chilu: P sitosterol 0,5mg/ ml methanol, lugng chim 4 ^1.
L&p mong: TLC silica gel 60 F254 Merck, hoat hda ban mdng d 105 V trong 1 gid
trudc khi sii dung.
Phdt Men: Dung dich acid sulphuric 10% trong ethanol, siy 105 " C trong 5-10
phiit, quan sat / VIS va UV366nm
Kit qud: Hinh 4.11(C, D).
Ca Ch 1
Hmh 4.11. sic ky d6 dinh tinh thanh phan nhom hoat chit dugc lieu kim ngan hoa {Flos Lonicerae).
Ky hieu: Acid caffeic (Ca); p- sitosterol (Si); acid chlorogenic (Ch); Cac raiu kim ngan hoa nghien cuu (vlt 1-3).
SKD dinh tinh din xuit acid caffeic (Hinh. A/UV366, B/UV366); SKD dinh tmh terpenoid (Hinh. C/VIS, Hinh. D/UV366).