Chi tu (fructus gardeniae) la qua chin phoi ben canh viec lam thudc, chi txi cdn dugc su
hay say kho cua cay danh danh Gardeniae dung phd bi6n trong nhan dan lam chit mau
jasminoides Ellis, la cay mgc hoang hoac thuc phim. Dl gop phin tieu chuin hda chit
dugc trdng tai nhiiu viing d mien Bac nudc lugng dugc lieu chi tu d Viet Nam, chiing tdi ta. Chi tu la vi thudc tir lau dugc su dung xin gidi thieu mdt sd phuong phap phan tich
trong y hgccd truyen dh diiu tri mdt sd benh dinh tinh cac nhdm hoat chit chinh cua chi tu
nhu sdt, vang da, huylt nhiet, tieu tien khd va djnh lugng geniposid bing ky thuat TLC khan, mit do dau, mieng khat... cd the diing va TLC- scarming va ap dung phuang phap dudi dang sao va phdi hgp vdi 1 sd vi khac phan tich da neu danh gia chit lugng va so (2) (6). TTianh phin hoat chat cua chi tu gdm sanh vdi mgt sd mlu chi tii tren thi trudng. cac chit thudc nhdm iridoid, sic td v^ acid 2. Nguyen lieu va phu-ong phap
huu ca... trong do geniposid, crocin va acid Ddi tugng khao sat gdm cac mlu chi tu clorogenic la cac thanh phan chinh (6). thu hai tai mdt sd vung d phia Bic va Ha Geniposid da dugc chung minh cd tac dung Ndi, do Khoa Tai nguyen cay thudc Vi?n bao ve gan, giai dgc, chong khdi u (1) (5). Dugc lieu curig cip va xac dinh ten khoa hgc. Crocin va crocetin cd tac dung chdng tang Cac mlu dugc liy-d giai doan qua chin va xu md mau (4), chdng ung thu bang quang (7), ly theo diing qui djnh (2). Mdt sd mau mua
tren thi trudng (Lan Ong, Ha Ndi) lam ddi tugng dl so sanh.
Chuan b; mau Mlu thir:
lg dugc lieu da dugc t ^ nhd, chilt vdi 10ml methanol, bing phucmg phdp siSu am 20 phiit, gan lgc liy phan d}ch chilt, thu dugc dung djch thu de djnh tinh.
Ddi vdi dung dich thu ap d\mg cho dinh lugng, each tiln hanh tuang t\r nhu tren, chiet nhic lai 3 lan, gdp djch chilt, them methanol vd 50ml, pha loang tiip vdi methanol de thu dugc dung dich cd ty le lg dugc lieu/ 500ml dung mdi.
Mlu ddi chilu: Dung dich geniposid 0,2 mg/ml trong methanol.
Dieu kifn phan tich
Dinh tinh iridoid va sic td crocin (crocin pigment).
Ldp mdng: Ldp mdng TLC plate silicagel 60 F254 Merck.
Lugng chim: 6fil mau ddi chilu (geniposid,
dung dich 0,5mg/val methanol) va 4-6^x1 mlu
thu.
He dung mdi sac ky:
N.butanol : acid acetic : nudc (7 : 2 :1) (dinh tinh iridoid).
Ethylacetat: isopropanol: nudc ( 6,5 : 2,5 : 1) (dinh tinh sic td crocin).
Phat hien: Quan sat dudi anh sang thudng va dudi UV 366imi (dinh tinh sic td crocin); Thudc thu anisaldehyd- acid sulphuric (AS) (5 ml anisaldehyd lin lugt trgn vdi 10 ml acid acetic glacial, 85 ml methanol va 5 ml acid sulphuric dam dac), siy d 105°C din khi xuit hien vlt (Dinh tinh iridoid),
Dinh lu-gng geniposid
HS thdng sic ky ldp mdng CAMAG gdm may chim kinh CAMAG- linomat 5, may do mat do densitometry CAMAG TLC SCANNER 3, dugc su dung vdi su ho trg ciia phan mem WinCATS. Ldp mdng HPTLC plat silicagel 60 F254 Merck, kich thudc 20x10. Cham
thang chuan geniposid va cac mau thii vdi lugng chim 2, 4, 6, 8 va 12 ^il cua dung dich mlu doi chilu v^ 4-10|al ctia dung dich mlu thir, chim nhic l^i 2-3 lin ddi vdi mdi mau thu. Ldp mdng dugc trien khai den 80mm vdi h? dung mdi ethylacetat: isopropanol: nudc (6,5 : 2,5 : 1) trong binh sic ky da dugc bao hda 30 phiit trudc khi triin khai. Kinh sau khi triin khai, dugc lam khd d trong hot