wnt catenin signaling in adrenocortical carcinoma

... whereas Frodo, another Dsh-binding protein, is required for Wnt/ ␤ -catenin signaling in the nucleus [14] These interactions may take place in various cellular compartments, linking specific activities ... DEP domains [13] Different domains of Dsh are engaged in specific interactions with different proteins, thereby leading to distinct signaling outcomes [13] Daam, a formin-related protein, promotes ... (d) DAPI staining of nuclei in the same field as in (c) Nuclear staining is marked by arrowheads (c,d) (e,f) The Ds3 construct, lacking amino acids 334-381, remained in the cytoplasm in the (e)...

Ngày tải lên: 06/08/2014, 18:21

... Veen et al 2004) 1.3 Wnt/ β -catenin pathway 1.3.1 Association of Wnt/ β -catenin pathway with chondrocyte dedifferentiation Dedifferentiation has been linked to Wnt/ β -catenin signaling pathway (Hwang, ... inhibiton of β -catenin transcriptional activity and rescued the suppression of collagen II induced by Wnt/ β -catenin activation (Hwang, Ryu et al 2004) β -catenin is an effector in the Wnt signaling pathway ... β -catenin influences distinct mechanisms operating Wnt- induced dedifferentiation, and Wntinduced differentiation 18 1.3.2 Curcumin as a Wnt/ β -catenin antagonist Signaling pathways can be blocked...

Ngày tải lên: 16/10/2015, 15:38

... Menin-JunD interaction involves the N-terminus (amino acids 1-40) and midregion (amino acids 323-428) of Menin and requires the N-terminus of JunD (amino acids 8-70) Menin’s tumor suppressor involves ... described in the literature data It is a point mutation in exon of MEN1 gene, in nucleotide 134 substituting Adenosine to Thymine, changing the Glutamic acid (GAG) to Valine (GTC) in the menin molecule ... MEN1 reading frame, resulting in a great deal of novel mutations, approximately 50%, in the index cases Mostly, they result in premature truncation of DNA sequence, inactivating menin protein The...

Ngày tải lên: 09/08/2014, 01:24

... Canonical Wnt/ beta -catenin signaling prevents osteoblasts from differentiating into chondrocytes Dev Cell 2005, 8:727-738 47 Day TF, Guo X, Garrett-Beal L, Yang Y: Wnt/ beta -catenin signaling in mesenchymal ... the WNT signaling pathway Consistently, we have detected, in the injured explants, upregulation of mRNA encoding the WNT/ β catenin transcriptional targets Axin-2 and c-JUN The WNT signaling pathway ... Mankin score 5) (j-m) Immunostaining for β catenin in parallel, non-consecutive sections of (h) and (i) (j-l) Indirect immunofluorescence stainings for β catenin from a parallel section in the...

Ngày tải lên: 09/08/2014, 08:22

... the β -catenin gene derived from DD samples does not contain such mutations [8] Given the proposed role of Wnt/ β -catenin signalling in DD, in this paper, we set out to examine Wnt expression in DD ... Korinek V, Barker N, Clevers H, Vogelstein B, Kinzler KW: Activation of beta -catenin- Tcf signaling in colon cancer by mutations in beta -catenin or APC Science 1997, 275(5307):1787-1790 Rubinfeld ... Birchmeier W: The ankyrin repeat protein Diversin recruits Casein kinase Iepsilon to the beta -catenin degradation complex and acts in both canonical Wnt and Wnt/ JNK signaling Genes Dev 2002, 16(16):2073-2084...

Ngày tải lên: 11/08/2014, 08:20

... CHAPTER 1: INTRODUCTION Zebrafish Fin Regeneration Wnt/ β -catenin Signaling Pathway Wnt/ β -catenin Signaling in Zebrafish Fin Regeneration Wnt/ β -catenin Signaling in Mammalian ... Weidinger et al 2007) Interestingly, disruption of β -catenin independent Wnt signaling affects fin regeneration as well Inhibition of the β -catenin independent Wnt signaling pathway results in increased ... β -catenin independent Wnt signaling completely blocks fin regeneration (Stoick-Cooper, Weidinger et al 2007) These findings suggest a model in which β -catenin independent Wnt signaling acts in...

Ngày tải lên: 24/08/2014, 11:36

... between Wnt and Hh signaling in the swimbladder development 4.7.1 Wnt signaling maintained Hh signaling and is negatively regulated by Hh signaling 4.7.2 Hh signaling might be required to maintain ... β -catenin/ Tcf signaling 1.7.8 Wnt antagonists and inhibitors 1.7.9 Wnt target genes 1.7.10 Wnt signaling in lung and lung development 1.7.11 Wnt signaling in Xenopus lung development 1.7.12 Wnt ... transgenic lines, which are engineered for heat-shock-inducible Wnt inhibition, the chemical inhibitor of Wnt signaling, IWR-1, and upregulation of Wnt signaling by knockdown of the Wnt protein inhibitor...

Ngày tải lên: 11/09/2015, 09:07

... Overview of Wnt/ β -catenin signaling pathway and its implication in oncology…….5 1.4 Strategies in inhibiting the Wnt/ β -catenin signaling pathway………………………16 1.4.1 Existing drugs as Wnt therapeutics……………………………………………17 ... protein; LRP: Low density lipoprotein receptor-related protein; WIFs: Wnt inhibitory factor-1 On the cell surface: Initiation of Wnt/ β -catenin signaling cascade Canonical Wnt/ β -catenin signaling ... directly squelching Wnt ligands, thus preventing FZD -Wnt binding, while the Dickkopf (DKK) family inhibits Wnt signaling by binding to LRP and Kremen receptors, and sterically hindering Wnt interaction...

Ngày tải lên: 11/09/2015, 10:18

... in total b -catenin, suggesting that a-defensins activate the b -catenin signaling pathway We then studied the role of the b -catenin signaling pathway in a-defensininduced increases in the proliferation ... an increase in the protein content of cyclin D and that quercetin inhibits the a-defensininduced increase in cyclin D protein content in HFL-1 lung fibroblasts (Fig S4), suggesting that cyclin ... a-defensin-induced increase in lung fibroblast proliferation and collagen synthesis * 0.5 * 0.4 * 0.3 0.2 Inhibition of b -catenin signaling pathway using quercetin blocked the a-defensin-induced increase...

Ngày tải lên: 18/02/2014, 06:20

... new insights into the role of pyruvate in tumor cells during hypoxia We show here that tumor HepG2 cells are inclined to maintain extracellular pyruvate at a constant level (0.4 mm) Hypoxia inhibits ... shown) This is in line with our previous work [29] indicating enhancement of glycolysis activity in the presence of pyruvate Increased glycolysis might thus maintain the ATP supply during oxygen ... protective effects against hypoxia and reoxygenation This is mainly ascribed to its ability to maintain redox status [17], intervening in the DNA repair system [18–20] and restoring antioxidant capacities...

Ngày tải lên: 18/02/2014, 16:20

... place in the order of minutes to hours and involves many hormones, including arginine vasopressin, angiotensin II, natriuretic peptides, vasoactive intestinal peptide, urotensin II, insulin and ... (within h) induced by hyperosmotic stress, indicating a role of this mitogenactivated protein kinase kinase kinase interacting protein for osmosensory signal transduction in fish 14-3-3 proteins ... of MAPK cascades, mitogen-activated protein kinase kinase kinase interacting protein (TAK binding protein ¼ TAB 2), as an IEG during hyperosmotic stress in tilapia gill epithelium [26] This gene...

Ngày tải lên: 18/02/2014, 16:20

... Thus, integrin-dependent cell volume sensing and signaling integrates into the overall context of insulin signaling Similarly, sensing of glutamine-induced hepatocyte swelling by integrins feeds into ... Osmosensing and signaling in mammalian cell function F Schliess et al [7] On the other hand, hyperosmotic shrinkage prevents insulin-induced hepatocyte swelling and proteolysis inhibition, indicating ... R, vom Dahl S & Haussinger D (2004) Involvement of integrins and Src ¨ in insulin signaling towards autophagic proteolysis in rat liver J Biol Chem 279, 21294–21301 15 Reinehr R, Becker S, Braun...

Ngày tải lên: 18/02/2014, 16:20

... work investigating the interaction of P2Y12 with P2Y1 signaling [25,40] We find that P2Y12 enhances the thrombin-induced Ca2+ response in a way not only involving adenylyl cyclase ⁄ PKA inhibition, ... (1993) ADP- and thapsigargin-evoked Ca2+ entry and protein-tyrosine phosphorylation are inhibited by the tyrosine kinase inhibitors genistein and methyl-2,5-dihydroxycinnamate in fura-2-loaded human ... where thrombin is generated in situ We find that (autocrine) ADP, acting via P2Y12, enhances and extends the thrombin-induced platelet activation by increasing Ca2+ mobilization from internal stores,...

Ngày tải lên: 18/02/2014, 16:20

... chemical inhibitor SB-431542 to block activin ⁄ TGF-b signaling in murine stem cell cultures Both experiments resulted in a strong growth inhibition of the stem cells but, fascinatingly, did not interfere ... cells into astrocytes, by binding and hyper activating the promoter of glial fibrillary acidic protein (GFAP) In parallel, BMP signals lead to enhanced expression of ID proteins, which in turn bind ... and BMP signaling cascades including Lefty1, ID1 and ID2 These data already indicate negative transcriptional regulation between LIF ⁄ STAT3 signaling on one end, and TGF-b ⁄ BMP signaling on...

Ngày tải lên: 19/02/2014, 00:20

... protein interactions In order to gain insight into the role of Hrs UIM-mediated ubiquitin signaling in cells, we performed a proteomic screen for Hrs UIM-interacting ubiquitinated proteins in human ... to bind clathrin via its C-terminal clathrin box motif and regulate clathrin-coated vesicle trafficking [57] Interestingly, Hrs also contains a C-terminal clathrin box motif that binds clathrin, ... ubiquitin binding of the ubiquitin-interacting motif J Biol Chem 278, 28976–28984 12 Miller SL, Malotky E & O’Bryan JP (2004) Analysis of the role of ubiquitin-interacting motifs in ubiquitin binding...

Ngày tải lên: 07/03/2014, 03:20

... protein (EGFP) reporter vector (pcDNA3 ⁄ EGFP-PPP6C-3¢UTR), in which the 3¢-UTR fragment of PPP6C, including the region encoding the putative binding site, was inserted downstream of the EGFP coding ... Gonadotropin-releasing hormone agonist-induced differences in granulosa cell cycle kinetics are associated with alterations in follicular fluid mullerian-inhibiting substance and androgen content J Clin ... had any effect on the intensity of EGFP fluorescence from the vector containing mutations in the region encoding the miR-373 binding sites These results show that miR-373 binds directly to the...

Ngày tải lên: 14/03/2014, 23:20

... (NF-jB) signaling The GnRH-induced signaling pathways in extrapituitary tissues are shown schematically in Fig Fig Schematic representation of GnRHR signaling in extrapituitary tissues Binding of ... adhesion kinase (FAK) and proline-rich tyrosine kinase (Pyk2) are the predominant mediators of integrin signaling GnRHR has been shown to signal through these molecules, suggesting a role for GnRH in ... gonadotropin releasing hormone receptor in human breast carcinoma Pathol Int 51, 333–337 24 Eidne KA, Flanagan CA, Harris NS & Millar RP (1987) Gonadotropin-releasing hormone (GnRH)-binding sites in...

Ngày tải lên: 16/03/2014, 04:20

... domain As in the binding studies with plant lectins, we routinely performed experiments to assess concentration dependence and inhibition of galectin binding by haptenic sugar Profiling of galectin ... chemiluminescence (top panel) Mass-spectrometric fingerprinting after digestion of the protein of this spot by trypsin ascertained identity to galectin-1, and quantification of the staining intensity in ... routing and binding activity The binding of b-galactoside-specific plant lectins intimated a new role, attributing to the integrin’s glycans the potential to become ligands for endogenous lectins...

Ngày tải lên: 16/03/2014, 10:20

... products were mixed in the following combinations: 18SVH–linker and linker) 18VL, 18VH–linker and linker)18VL, 21SVH–linker and linker)21VL, 21VH–linker and linker)21VL and singlechain antibodies, ... protein kinases and regulatory molecules into the membrane-receptor complexes, resulting in the correct initiation and amplification of the signaling reaction WASP is an adaptor molecule containing ... blotting, immunostaining, immunoprecipitation and blocking of protein function Therefore, if intrabodies retain their specificity and high-affinity binding properties, they may be useful tools for inhibition...

Ngày tải lên: 16/03/2014, 14:20

... and Sox9 Mol Endocrinol 18, 2451–2462 39 Kim YH, Choi CY & Kim Y (1999) Covalent modification of the homeodomain-interacting protein kinase (HIPK2) by the ubiquitin-like protein SUMO-1 Proc Natl ... Billig H (2004) Inhibition of small ubiquitin-related modifier-1 expression by luteinizing hormone receptor stimulation is linked to induction of progesterone receptor during ovulation in mouse granulosa ... by inserting the corresponding fragments into pcDNA-Tag3A (Stratagene, La Jolla, CA, USA) cleaved with EcoRI and HindIII The BsrGI-BamHI fragment from pEGFP-LRH1-K213 ⁄ 289R was inserted into...

Ngày tải lên: 23/03/2014, 06:20