... according tothe data available and the role of the aquifer inthemanagement of thebasin and the degree of detail desired inthe results Inthe cases of the basins analyzed, it was necessary to ... measures in WRS inthe area of theMediterranean basins inthe region of Valencia These basins are managed basically by two basin agencies: the Hydrographical Confederation of the River Júcar and the ... management organizations in Spain Continuing with the methodology of the analysis described inthe previous section, the Aquatool environment provides the following tools: The first point of the...
... rNetPhotosynthesis ¼ xNPSi i¼1 n ; where xNPSi describes the integral of carbon uptake per hour Data points were spline-interpolated to obtain timecontinuous information on net photosynthesis during the ... et al ¨ how to engineer plant metabolism in order to improve freezing tolerance, one could suggest increasing the maximum activities of enzymes participating in sucrose cycling Assuming that Rsch ... homologous to 514 the phloem loading transporter AtSUC2 [20,24] However, it has to be kept in mind that the sink export rate in our model is composed of assimilate export to sink organs and flux into...
... oxygen in argon is used, rather than pure oxygen, avoiding the use of highly flammable or explosive gases inthe laboratory The oxygen is added either inthe spray chamber or using a T-connector ... eventually leading to clogging of the cone orifice and a reduction in sensitivity To prevent carbon deposition, the carbon inthe sample is Figure Visual optimization of the oxygen level inthe plasma ... and, inthe case of the sample uptake tubing, care must also be taken to avoid contamination of the sample through contact with the tubing material For routine analysis of organic solvents, the...
... housing or offices Most seismic and flood- or wind-resistant building codes inthe region strive to protect the lives of those inside the building, not to ensure the continuity of the building’s ... non-ambulatory inpatients to survive; instead, these patients must continue to receive appropriate care even as new patients are coming in as a result of the injuries sustained during the event ... capacity Training and Education It is likewise vital to promote the inclusion of disaster mitigation in all training programs related tothe construction, maintenance, administration, financial management, ...
... itself to point estimation, hypothesis testing, and classification of objects based on their reflectance spectra is needed In addition to minimizing problems of data mining and colinearity, the ... , k ≥ 48 The arrow indicates the accepted truncation point of the power spectra at the 42th frequency The horizontal dashed line indicates the 5% significance level under the null hypothesis of ... quite well by the model in (3) The non-linear model explained over 98% of the variation within individual power spectra Residual variances declined initially rapidly with increasing frequency...
... 60.0% sessile oaks The seeds were germinated in an incubator, and then transferred tothe nursery of Pierroton (Bordeaux) The progenies were randomly distributed inthe nursery, in one unitary parcel ... over all the individuals and all the characters (DFAa) separated the seedlings into two groups along the first axis This axis explained 33% of the total variance and the second, 6% The characters ... However, the larger part of the differences between groups was found in char- acters linked tothe size of the leaf rather than tothe discrimination between species In contrast, when the morphological...
... agarose (normal melting point; Invitrogen) was filled into the wells of a 48-well plate Cylinders of a defined size were created by inserting a self-manufactured metal-pin plate into the hot agarose ... [15], allowing the exact assignment of the observed effects to SFB Taken together, the present system allows for the first time to simulate the initial cartilage destruction in rheumatoid joints mediated ... cartilage to reduce the complex cellular network tothe main elements of interest The focus of the model was the representation of initial cartilage destruction, thereby reflecting the main features...
... processing, minimizing variance, and the ability to quantify the actual stained area together with staining intensity in a time efficient manner [8,9] This allows analysis of large numbers of stained ... out of the 10 patients inthe prednisolone group responded versus none of the nine patients inthe placebo group To determine whether the same observer identified the same differences inthe synovial ... threshold levels regarding the detection of stained antigen, nuclear staining and background staining After the analysis, all observers independently calculated the mean change inthe total number of...
... are built into the assumptions of our gene pool sampling procedure using isofemale strains Sampling of hypothetical isofemale strains was simulated according tothe steps outlined in figure For ... together and are more similar to strains and 10 than the other strains Strains and 10 share the fact that they are segregating one l A allele and three A alleles For the remaining three strains, ... strain inthe second cluster and was one of the strains in l which the frequency of A was 0.75 In figure B, strain 10 joins after the second and third cluster are combined Other changes in strain...
... groupings based upon the rate of change of VL and the presence of mutation Testing the hypothesis The preliminary work relating to testing this hypothesis requires longitudinal data that includes ... patients VL points should be collated to produce pairs of VL measurements relating tothe start and end points of an increase in log10 (VL) of greater than log – In keeping with the clinical practice ... ascertained, rates of change in VL can be compared to a threshold value in order to decide whether the patient is likely to have been adherent during the period shortly before and during the spike...
... SBEIIa-D(A201T) the amino acid change induced by the EMS treatment is located inthe region of the N-terminal domain of the glycogen branching enzyme family, reported to be essential for the size of the ... for other genes involved in starch biosynthesis Further increase in amylose content was also observed for the three double null lines obtained from the cross of the three single null mutants In ... substrate binding sites and inthe protein secondary structure In Figure we show as an example the case of SBEIIa-D (V398I) : while in wild type protein residue 398 was involved inthe b3 strand of the...
... cultured in ml RPMI 1640 at 37°C and 5% CO2 for 16 h inthe presence or absence of the individual chemotherapeutic agents including carboplatin, vinorelbine or gemcitabine These cytotoxic drugs ... cytotoxicity assay using 2-photon microscopy in two separate experiments To analyze the effects of conventional chemotherapy in STST, each of the chemotherapeutic agents including carboplatin, ... flow cytometry in human Caspase-3 induction determined by flow cytometry in human cancer cell lines inthe absence (RPMI) or presence of carboplatin (CARB), vinorelbine (VIN) and gemcitabine (GEM),...
... settings for smoothing (n = [11-point] smoothing with rounds) In addition, we attempted to optimize the settings by trying varying levels of smoothing, including 7-point and 13point, which produced ... Grunstein M: In vivo protein-protein and proteinDNA crosslinking for genomewide binding microarray Methods 2003, 31:90-95 Wells J, Farnham PJ: Characterizing transcription factor binding sites using ... event, and none of the noise, in a single window Therefore, in most ChIP-chip experiments the window size should be adjusted to approximately the average shear size of the chromatin The average shear...
... demand by hyperlinking them tothe corresponding well icons inthe plate plot The reader is directed tothe online complement [32] for an interactive example Using plate plots in this way provides ... discrimination of these contaminants from single, living cells during data acquisition, and hence they can end up inthe raw data To a certain extent we can discriminate contaminants from living ... analysis of the raw data in order to integrate efficiently the information collected Analyzing and managing the vast amount of data generated in these studies initially seems to be a daunting task...
... (i.e., inthe top α%) genes in each of these patients We can formulate this in mathematical terms Let I(egi ,pj ) be an indicator function that outputs a value if gi is inthe top α% of the genes in ... (2.14) The numerator of the eq.2.14 represents the number of ways of sampling k DEGs inthe pathway from K observed DEGs inthe microarray and the number of ways of sampling (n − k) non-DEGs inthe ... because the t-statistic becomes artificially in ated when the standard-error term inthe denominator is very small The value for s0 is chosen to minimize the coefficient of variation Note that in the...
... The usefulness of the method was demonstrated by detecting the major components in aminoglycoside antibiotic mixtures Gentamicin was separated into several peaks in 15 minutes, and neomycin into ... of the inclusion complexes formed between the carrier and the solute was found to be an important factor affecting stacking efficiency in both the conventional field amplification sample injection ... (LOD) in micellar electrokinetic chromatography (MEKC) In this mode a displacer plug was introduced before sample injection to reduce the length of the concentrated sample zone and increase the...
... [6] In pressure injection, the inlet end of the capillary is immersed inthe sample and the other end inthe buffer Sample is introduced by either pressurizing the inlet vial containing the sample ... elevating the inlet end of the capillary relative tothe outlet end These two injection methods can be generalized as hydrodynamic injection The volume, v, of the sample injected into the capillary ... on-site without having to store and transport the samples back to a laboratory for testing The clinical field also increasingly demands portable instruments To defend against highly infection diseases...
... However, the application of Bayesian modelling scheme into the interpretation of Microarray dataset is yet to be investigated In this thesis, we have used this machine learning to study the application ... Location of all the original examples inthe feature space Location of all the training examples inthe feature space Location of all training and testing examples inthe feature space ... will interact with probes If there is an interaction, single strands from targets and the single strand of the cDNA will combine and the targets will stick onto the immobilized probes, binding the...
... 42-mer: adenosine: 2’-deoxyguanosine: thymidine to 3:1:1:1 The quantity of 42-mer used in this extraction was three times in excess relative to adenosine This aided in increasing the interaction ... proposed here that the 42-mer of the adenosine was to be dissolved inthe IL to induce specificity in extracting adenosine from an aqueous sample There were several advantages of using this method ... Prior to drying of the [C4MIM]PF6, the aqueous 42-mer was added to it However, the 42-mer was found to be insoluble inthe [C4MIM]PF6 despite subsequent heating at 50 ˚C and vortexing for 30 The...