uplc esi ms and uplc appi ms analysis of intact non derivatized steroids
Bio nanoparticles and bio microfibers for improved gene transfer into glioma cells
... ingredients and angle of the atoms involved 1.1.3.2 Applications of Self-assembled Polyelectrolyte Microfiber One of the cornerstones of this study is the formation of self-assembled microfiber driven ... findings were presented in this thesis VIII List of Figures and Tables LIST OF FIGURES AND TABLES Figure Page Figure 2.1 - Endosomolytic Tat peptide and ternary magnetofection complexes………………………………………… ... Kost and Condreay, 2002; Li et al., 2005; Sandig et al., 1996) due to the vulnerability of BV in the presence of serum factors (Hofmann et al., 1998; Hofmann et al., 1999) The susceptibility of...
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Báo cáo y học: "In vitro bactericidal activities of Japanese rice-fluid against Helicobacter pylori strains"
... consisting of three kinds of Rice-fluid derived from the two kinds of raw rice; i e., polished (pH6.32) and unpolished (pH5.98), and the usually cocked rice (pH5.91), and remaining one compound of the ... observed against other four strains of E coli, five strains of S aureus, S epidermidis, E faecalis and P aeruginosa, and three strains of K pneumoniae and two strains of K oxytoca, respectively (data ... activities of the three kinds of Rice-fluid against various species of microorganisms including H pylori strains by the timekill-curve assay [14, 16] Every kind of Rice-fluid derived from unpolished and...
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Báo cáo khoa học: NovelN,N¢-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery – synthesis,in vitro transfection activity, and physicochemical characterization docx
... laboratory and the structureactivity data collected for the various series contribute to the long-term goals of understanding the mechanism of lipofection, and the rational design of pharmaceutically ... active derivative 1,3lb4, of lipid mixing of the active compounds with anionic vesicles Lipids of the 1,3lb series were synthesized with the intent of enhancing the transport of exogenous genetic ... sequentially with 100 mL chloroform, and 1, 3, 5, 7, 8, 9, 10, 12 (300 mL) and 15% methanol chloroform The 10% and 12% fractions were combined and concentrated to give 2.79 g (68%) of N,NÂ-ditetradecanoyl-1,3-diaminopropyl-2carbamoyl-[bis-(2-dimethylaminoethane)]...
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Báo cáo Y học: In vitro gene therapy of mucopolysaccharidosis type I by lentiviral vectors pdf
... increased enzymatic activity, both in the absence of polybrene (means of 6, 22 and 117 nmolÆh)1Æmg)1) or in the presence of polybrene (means of 20, 32, and 155 nmolÆh)1Æmg)1) The IDUA activity obtained ... end and the other at the 5¢ end of the vector genome After the first amplification (lanes 1–3) no band was visible The nested reaction (lanes 4–6) amplified a fragment of 166 and 121 bp at-3¢ and ... Winchester, B.G (2001) Mutational analysis of 85 mucopolysaccharidosis type I families: frequency of known mutations, identification of 17 novel mutations and in vitro expression of missense mutations Hum...
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Báo cáo khoa học: The in vitro nuclear aggregates of polyamines pot
... ivNAPs of the molecular masses of several coeluting compounds On the other hand, attempts to confirm the proposed molecular formulas by means of ‘soft’ MS techniques (MALDI-TOF and ESI- MS, in ... possibility of cocrystallization in the genesis of the crystallites Cocrystallization of polyamines and phosphates seems to be less probable than crystallization of ivNAPs, on the basis of the following ... containing 40 lL of m NaOH and 60 lL of a saturated solution of NaHCO3 Polyamines were derivatized with dansyl chloride as previously described, and separated by RP-HPLC Derivatization and analysis were...
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báo cáo hóa học:" The effect of abductor muscle and anterior-posterior hip contact load simulation on the in-vitro primary stability of a cementless hip stem" ppt
... Description of application J Biomech 1972, 5(3):283-93 37 Cristofolini L, Vinteconti M: Towards the standardization of in vitro load transfer investigations of hip prostheses Journal of Strain Analysis ... [10] We hypothesise that simulation of an abductor muscle force increases implant micromotion and migration of cementless stems compared with hip contact forces alone The effect of the anterior-posterior ... alone Axial and torsional loading of the actuator produced distal (Fd), medial (Fm) and anterior-posterior (Fap) loading of the femoral head due to the mounting geometry and the offset between...
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Báo cáo vật lý: "Evaluation of In Vitro Antioxidant Activity of 5H-dibenz[b,f]azepine and Its Analogues" ppt
... mass spectroscopy and elemental analysis The IR spectra of compounds (e) and (f) showed the absent of the N–H absorption band at 3400 cm–1 and the presence of the C=O stretching band at 1600 cm–1, ... chemistry and antioxidant properties of 5H-dibenz[b,f]azepine and its derivatives Six molecules (a–f) were synthesised, and their structures were established by chemical and spectral analysis ... AA was also determined and compared with those of the synthesised compounds (Fig 3) The antioxidant activity of BHA and AA was still lower than that of the compounds (a) and (d) In general, the...
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Báo cáo khoa học: "Effect of Reproductive Status on In Vitro Developmental Competence of Bovine Oocytes" docx
... J and Lu, K H Effects of different oestrus stages of ovaries and sizes of follicles on generation of bovine embryos in vitro Theriogenology 1990, 33,335 Taylor, C and Rajamahendran, R Effect of ... embryos: facts and artifacts Human Reproduction Update 1995, 1, 91-148 Behboodi, E., Anderson, G B and BonDurant, R H Development of in vitro fertilized oocytes from pregnant and non pregnant cows ... Spitzer, A and Batt, P A Enhanced rate of cleavage and development for sheep zygotes cultured to the blastocyst stage in vitro in the absence of serum and somatic cells: amino acids, vitamins and culturing...
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Báo cáo lâm nghiệp: "In vitro clonal propagation of Acacia mangium Willd. and its evaluation of genetic stability through RAPD marker" ppsx
... test at the level of 5% + Callusing at the basal end Table III Analysis of variance for the effects of BAP, Ads, NAA and IAA on production of multiple shoots (A) and average number of shoots per ... (Fig 1b) Analysis of variance for the effects of cytokinins and auxins on production of multiple shoots and average number of shoots/explant was presented in Table III The interaction of two cytokinins ... were excised and cultured on half-strength MS basal salts supplemented with 0, 0.25, 0.5, 1.0 and 1.5 mg/L of IAA, IBA and NAA alone or in combination for induction of rooting In case of rooting...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... chondrogenesis of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene Materials and methods Construction and preparation of recombinant ... been implicated in embryogenesis and morphogenesis of various tissues and organs, where they regulate growth, differentiation, chemotaxis and apoptosis of a variety of cell types, including mesenchymal, ... manuscript and contributed to the study design, data analysis, interpretation of data and drafting and revision of the manuscript The data have been generated by AFS, BP, MK, SCG, and a data...
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Báo cáo y học: " In-vitro antiviral activity of Solanum nigrum against Hepatitis C Virus" pdf
... analyze the effect of SN against HCV NS3 gene, cDNA was synthesized with μg of RNA, using Revert Aid TM First Strand cDNA Synthesis Kit (Fermentas, St Leon-Rot/Germany) Gene expression analysis was ... http://www.virologyj.com/content/8/1/26 Page of 120 100 80 60 40 20 Cont SNC SNH Concentration (ug) Figure Anti-HCV activity of chloroform and n-hexane fraction of Solanum nigrum Huh-7 cells were incubated with HCV serum and 100 ... plate was sealed and kept at 37°C in an atmosphere of 5% CO2 for 24 h After the herbal extracts treatment was over, removed the media and test compounds 100 μl fresh media and 20 μl of MTT solution...
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Báo cáo y học: "The triple combination of tenofovir, emtricitabine and efavirenz shows synergistic anti-HIV-1 activity in vitro: a mechanism of action study" pdf
... alone, and 88 design points in quintuplicate for a total of 440 data points were used in the modeling The analysis started with modeling of the error structure and calculation of variance and means ... A combination of decreased NRTI incorporation and decreased excision determines the resistance profile of HIV-1 K65R RT Aids 2005, 19:1751-1760 Menendez-Arias L: Mechanisms of resistance to nucleoside ... the non- parallel and converging lines at the left of the y-axis (Table 3) To reduce the possibility of analysis bias, we further studied the combination of TFV-DP+FTC-TP using MacSynergy II analysis, ...
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Báo cáo y học: "In vitro nuclear interactome of the HIV-1 Tat protein" pps
... interpretation and in silico analysis, and drafted the manuscript LG performed GST-pull down/ Western blotting experiments NOD participated in the experimental design and performed LC MS/ MS and peptide and ... experimental design and supervised the proteomic analysis NS participated in the interpretation of results and final editing of the manuscript WWH supervised the study design, execution, analysis and revised ... intense ions to determine charge state prior to MS/ MS analysis Data analysis All MS/ MS spectra were sequence database searched using TurboSEQUEST The MS/ MS spectra were searched against the redundant...
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Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... Aratani Y, Kitagawa Y 1988;Enhanced synthesis and secretion of type IV collagen and entactin during adipose conversion of 3T3-L1 cells and production of unorthodox laminin complex J Biol Chem ... combination of protein profiling and isotopomer analysis using matrixassisted laser desorption/ionizationtime of flight mass spectrometry reveals an active metabolism of the extracellular matrix of 3T3-L1 ... regulates adipogenesis during mammary gland involution J Cell Biol (4):693-703 110 Zizola CF, Julianelli V, Bertolesi G, et al 2007;Role of versican and hyaluronan in the differentiation of 3T3-L1 cells...
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báo cáo hóa học: " Beyond platinum: synthesis, characterization, and in vitro toxicity of Cu(II)-releasing polymer nanoparticles for potential use as a drug delivery vector" ppt
... at the University of North Texas Authors’ contributions AH carried out Cu loading and release studies via ICP MS, participated in the design and coordination of ICP MS studies, and helped draft ... reaction used in the synthesis of polymeric nanoparticles MC carried out particle synthesis, and metal loading RP conceived of the study, and participated in its design and coordination, carried ... toxicity of CuCNPs upon reduction of Cu content water, which has a neutral to slightly acidic pH While this feature is promising in terms of the stability of solutions of CuCNPs over long periods of...
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báo cáo hóa học:" The Photodynamic Effect of Different Size ZnO Nanoparticles on Cancer Cell Proliferation In Vitro" docx
... Ministry of Science and Technology (2007AA022007 and 2008DFA51180) and the Natural Science Foundation of Jiangsu Province (BK2008149) Open Access This article is distributed under the terms of the ... absence of UV irradiation: a ZnO nanoparticlefree (control), b 2.5 lg/mL of ZnO nanoparticles, c 5.0 lg/mL of ZnO nanoparticles and d 10.0 lg/mL of ZnO nanoparticles; b The 2.5 10 Concentration of ... the absence of UV irradiation: a ZnO nanoparticlefree (control), b 2.5 lg/mL of ZnO nanoparticles, c 5.0 lg/mL of ZnO nanoparticles and d 10.0 lg/mL of ZnO nanoparticles; b The Derease of cell activity/%...
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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" doc
... cm2) were punched in the agar plates and filled with 100 ll of either antibiotics or their respective Gnps conjugated forms The concentrations of both the forms of antibiotics were at their respective ... was the key factor for the synthesis of Gnps below 20 nm (dh) We used the ratio of reductant and H[AuCl4] in such a way that the synthesized Gnps produced a size of 13.54 nm (Fig 2a) when measured ... number distribution of the hydrodynamic diameter of bare Gnps and Gnps conjugated antibiotics (b) represents the intensity distribution of the hydrodynamic diameter of the free Gnps and the antibiotics...
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Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" pdf
... cm2) were punched in the agar plates and filled with 100 ll of either antibiotics or their respective Gnps conjugated forms The concentrations of both the forms of antibiotics were at their respective ... was the key factor for the synthesis of Gnps below 20 nm (dh) We used the ratio of reductant and H[AuCl4] in such a way that the synthesized Gnps produced a size of 13.54 nm (Fig 2a) when measured ... number distribution of the hydrodynamic diameter of bare Gnps and Gnps conjugated antibiotics (b) represents the intensity distribution of the hydrodynamic diameter of the free Gnps and the antibiotics...
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báo cáo khoa học: "Cationic nanoparticles for delivery of amphotericin B: preparation, characterization and activity in vitro" potx
... characterization, and thermal behavior of layer-by-layer deposited ultrathin films of poly(vinyl sulfate) and poly(allylamine) Langmuir 1993, 9:481-6 Decher G, Hong JD: Buildup of ultrathin multilayer films ... non- responsiveness of the dispersion indicative of absence of inner vesicle compartment [29]; (ii) TEM micrographs with electronic staining [30]; (iii) cryo-TEM micrographs [31]; (iv) fluid and ... effect of [PDDA] on sizes and zeta-potentials of DODAB BF/AmB/CMC assemblies at mM DODAB, 0.005 mM AmB and mg/mL CMC is on Figure The region of PDDA concentrations for size minimization and high...
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In vitro and in vivo evaluation of transferrin conjugated lipid shell and polymer core nanoparticles for targeted delivery of docetaxel
... synthesis and characterization of lipid shell and polymer core nanoparticles (LPNPs) are discussed in Chapter while the synthesis and characterization of transferrin conjugated lipid shell and ... Mr Gan Chee Wee, Ms Chaw Su Yin, Mr Tan Yang Fei, Mr Mi Yu, Ms Zhao Jing, Mr Annandh and Dr Mutu, for their support and advices i TABLE OF CONTENTS ACKNOWLEDGEMENTS i TABLE OF CONTENTS ii SUMMARY ... because of the higher iron demand for faster cell growth and division of the malignant cells (Vyas and Sihorkar , 2000; Li and Qian, 2002) Transferrin plays a pivotal role in the transportation of...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25