tse markers in vitro assays as indirect measurements of infectivity
Báo cáo y học: " In vitro bioassay as a predictor of in vivo response" pdf
... native analogues in their affinity for binding proteins In view of this, an important practical consequence of our simulations results are that the testing of newly designed hormones in in vivo systems ... LM, Mercado M, Baumann G: Influence of high affinity growth hormone binding protein on plasma profiles of free and bound GH and on the apparent half-life of GH J Clin Invest 1993, 91:629-641 Baumann ... M, Franks S: Diurnal variation of sex hormone binding globulin and insulin-like growth factor binding protein-1 in women with polycystic ovary syndrome Clin Endocrinol (Oxf) 1995, 43:159-165 Publish...
- 8
- 251
- 0
Báo cáo khoa học: Recent contributions of in vitro models to our understanding of hepatitis C virus life cycle pdf
... selection of quasispecies during the culture of infected hepatocytes To succeed in obtaining HCV infection in primary human hepatocytes, an existing model of hepatitis B virus infection was used ... association of HCVcc with lipoproteins might not be as optimal as in vivo passage of this virus in chimpanzees or in mice containing human liver xenografts In fact, enhanced virus infectivity ... are at the N-terminus of the NS3 helicase, in two distinct positions of NS4B, in the centre of NS5A and in the C-terminal region of NS5B [3–6] The significance of these mutations has been questioned...
- 14
- 532
- 0
Characterization of Spin Coated Polymers in Nano-environments as a Function of Film Thickness pot
... morphological behavior of polymer brush films Industrial use of thin films has increased for several reasons including the development of ever-smaller electronic devices As applications of polymers become ... would dominate the behavior of the polymer Wallace used similar reasoning stating that the interactions with the surface could act as a crosslink in the polymer by restraining certain chains.11 ... material by finding the inflection point in the Tg step 3.1.3.2 Gel Permeation Chromato graphy A 3.160 mg/mL solution of PMMA in chloroform was prepared One sample of 100 µL was injected into a Waters...
- 80
- 375
- 0
Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" doc
... summarized in Table As seen in the Table 1, kanamycin in Gnps conjugated form was more effective than its free form in the case of E coli DH5a and S aureus, whereas streptomycin was more effective in ... mode of action of the antibiotics Ampicillin inhibits the cell wall biosynthesis by inhibiting the cross-linking reaction mediated by transpeptidase, while both streptomycin and kanamycin bind ... delocalization of the electron in the carbonyl group of the b-lactam ring in ampicillin at elevated temperature Elevation in temperature might induce breakage in the b-lactam ring of the free ampicillin,...
- 9
- 332
- 0
Báo cáo hóa học: " In Vitro Structural and Functional Evaluation of Gold Nanoparticles Conjugated Antibiotics" pdf
... summarized in Table As seen in the Table 1, kanamycin in Gnps conjugated form was more effective than its free form in the case of E coli DH5a and S aureus, whereas streptomycin was more effective in ... mode of action of the antibiotics Ampicillin inhibits the cell wall biosynthesis by inhibiting the cross-linking reaction mediated by transpeptidase, while both streptomycin and kanamycin bind ... delocalization of the electron in the carbonyl group of the b-lactam ring in ampicillin at elevated temperature Elevation in temperature might induce breakage in the b-lactam ring of the free ampicillin,...
- 9
- 285
- 0
Báo cáo khoa học " In vitro cytostatic and immunomodulatory properties of the medicinal mushroom Lentinula edodes " pdf
... proliferation was measured by incorporation of [3H]-thymidine expressed in counts per minute (cpm) In each of at least two experiments, means of the counts per minute (cpm) for each set of replica ... extracts was carried out for the following reasons: The production of fruit bodies and mycelium in L edodes as well as in many other medicinal mushrooms, comprise the two main production methods (Wasser ... t-test) In Figs and asterisks above data points indicate significant differences from the control In the LTT test, the means of SI in repeated testing of the extracts were evaluated by analysis of...
- 8
- 383
- 0
Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
... sulp ASB/SBP ni detulid erew seidobitna ehT seidobitna yramirp fo gnidnib cificeps-non eht kcolb ot Co73 ta nim 001 rof )ASB/SBP( ASB %4 gniniatnoc SBP ni )ASU ,demyZ( mures taog %2 dna )ASU ,LRB-ocbiG( ... ecnanetniam eht dna euqinhcet reporp eht ,erutluc yramirp ot gnilpmas morf emit eht taht thguoht saw tI ]72,22[ srotinegorp citeiopotameah rof esac eht sa emas eht ,srotinegorp lamyhcnesem ni ... erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP htiw desnir erew )2 egassap doolb droc( sllec ,gnilebal PAFG dna ESN lacimehcotyconummi eht roF )ASU ,nocimehC ;PAFG(...
- 6
- 410
- 0
Báo cáo khoa học: "Variation of vessel lumen diameter in radial direction as an indication of the juvenile wood growth in oak" pptx
... dominant tree) For latewood vessels, with increasing age of growth rings, an increase in the lumen diameter and in the number of vessels per unit area was observed (table III) However, an increase ... remained more or less constant with any further increase of the cambial age of growth rings Increase in the earlywood vessel lumen diameter in the outerwood was significant compared with that in ... from each of these rings for measurements The lumen diameter of earlywood and latewood vessels was measured on samples to include all the growth rings under study Measurements were made using the...
- 8
- 341
- 0
Báo cáo y học: "In vitro model for the analysis of synovial fibroblast-mediated degradation of intact cartilage" pptx
... embedding of the discs, a total of 450 μl hot, liquid, 2% agarose (normal melting point; Invitrogen) was filled into the wells of a 48-well plate Cylinders of a defined size were created by inserting ... represent the dose of each cytokine with the maximum effect in monocultures of stimulated SFB (concerning the induction of several matrix-metalloproteinase (MMP), as determined in initial experiments, ... structures in areas near the cartilage surface (Figure 4k1 to k4) Invasion of synovial fibroblasts into the cartilage Using light microscopy, an invasive behaviour of co-cultured SFB was not observed in...
- 20
- 524
- 0
Báo cáo y học: "Shared expression of phenotypic markers in systemic sclerosis indicates a convergence of pericytes and fibroblasts to a myofibroblast lineage in fibrosis" pdf
... Myofibroblasts are present only in fibrotic dcSSc skin The distribution of myofibroblasts was investigated using the 1A4 monoclonal antibody against α-SMA In normal skin, αSMA immunostaining was ... collagen and elastic fibres in the dermis (Fig 2a) In four dcSSc cases, an increase in LOX immunostaining was observed when compared with normal skin, principally in interstitial fibroblastic cells ... ED-A FN was localized to the presence of myofibroblasts Increased immunostaining for ED-A FN was located predominantly in the reticular dermis mirroring the distribution of myofibroblasts (Fig...
- 11
- 397
- 0
Báo cáo khoa học: "In vitro studies on the modification of low-dose hyper-radiosensitivity in prostate cancer cells by incubation with genistein and estradiol" pot
... protein extraction The shown results represent one of three assays In controls incubated in FCS containing media and in controls incubated in media with FCS-withdrawal there was a induction of ... Saitoh JI, Takahashi T, Mitsuhashi N: Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro: possible involvement of inhibition of survival ... use of FCS-containing media during genistein incubation and the incubation with higher genistein doses Mechanisms of interaction To search for mechanisms of interaction we investigated protein...
- 12
- 368
- 0
In vitro and ex vivo effect of hyaluronic acid on erythrocyte flow properties pps
... fluidity was assessed using the parallel component of the nitrogen hyperfine tensor of 5-SASL (T//) as a representative parameter of lipid chain rigidity Thus, increased T// values are indicative of ... reversibility of HA-erythrocyte interaction, RI was determined again in erythrocytes of each sample after washing twice with PBS -Aggregability determination in erythrocytes incubated in HA Blood ... Also involved in drafting the manuscript, revising it critically and in giving final approval of the version to be published LU: acquisition, analysis and interpretation of data of laboratory assays...
- 7
- 221
- 0
Báo cáo y học: "Captopril reduces cardiac inflammatory markers in spontaneously hypertensive rats by inactivation of NF-kB" ppsx
... and vascular dysfunction both in vivo and in vitro, suggesting a protective action of IL-10 in vascular diseases such as arterial hypertension [14] In fact, IL10 attenuates the increases in vascular ... reduction in the plasma levels of IL-10 An increase in circulating pro-inflammatory markers has been found in hypertensive patients [38] and in patients with pulmonary arterial hypertension [39], as ... well as in SHR [40], and in NO-deficient rats [8] In contrast, a decrease in plasma levels of IL-10 has been found in patients with coronary artery disease and arterial hypertension [41], and in...
- 9
- 301
- 0
In vitro metabolic drug interaction study of warfarin with sildenafil citrate
... Table 4.5 In vitro effect of sildenafil on the protein binding of (RS)warfarin in pooled rat liver microsomes 93 Table 4.6 In vitro effect of warfarin on the protein binding of sildenafil in pooled ... S-warfarin has higher binding affinity for the primary binding sites [50, 51] while R-warfarin has greater binding affinity for the secondary binding sites [52] The serum protein binding affinity of (R)-warfarin ... for in vitro effect of warfarin on the rat serum protein binding of sildenafil 89 Figure 4.3 Graphs for in vitro effect of sildenafil on the rat liver microsomal protein binding of warfarin enantiomers...
- 252
- 269
- 0
Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... diluting of packed cells of each type with 1.5 ml of distilled water Osmotic fragility was studied for each drug concentration In vitro releasing study The release of pravastatin as well as hemoglobin ... the loading of pravastatin into erythrocytes was directly proportional with increase of drug concentration in the incubation medium, in the 2-10 mg/ml concentration range This finding is in concurrence ... statins including inhibition of the metabolism [5], administration of statins with certain juices [6] or inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase...
- 9
- 829
- 0
Tài liệu Báo cáo khoa học: Trophoblast-like human choriocarcinoma cells serve as a suitable in vitro model for selective cholesteryl ester uptake from high density lipoproteins pdf
... early trophoblast of the attachment phase or as later invasive stage [46–48] Thus, in most cases, choriocarcinoma has the appearance of trophoblast, being predominantly syncytiotrophoblastic or cytotrophoblastic ... measurement was performed as described [31] Specific binding (4 °C) was calculated as the difference between total and nonspecific binding [37] To determine cell-associated (the sum of binding and internalization) ... serum albumin as a standard Oxidation of LDL (ox-LDL) was performed with 1.66 lM of Cu2+ in NaCl/Pi as described [32] Lipoprotein labeling procedures Single-labeling (a) Iodination of HDL3 with...
- 12
- 470
- 0
báo cáo hóa học:" Can urinary exosomes act as treatment response markers in prostate cancer?" ppt
... study, investigating urinary exosomes in prostate cancer patients We had two main aims in the study; firstly to assess the feasibility of using urine as an exosome source in the context of a clinical ... by changes in urinary exosomes In this report, we present a pilot study with the key aim of evaluating the feasibility of studying urine exosomes of PCa patients, as tools for monitoring response ... tubes; a measure of whether exosomes remain attached to the bead surface Results are expressed as the ratio of Calcein: Class-I fluorescence Examining proteolytic damage of exosomes by urine Exosomes...
- 13
- 445
- 0
báo cáo hóa học: " Beyond platinum: synthesis, characterization, and in vitro toxicity of Cu(II)-releasing polymer nanoparticles for potential use as a drug delivery vector" ppt
... the introduction of chloride increased the rate of Cu release in acetate buffer (Figure 3C) Again, Cu release was highly dependent on the identity of competing ligand as illustrated by comparing ... effect of competing ligands on Cu release with the idea that the underlying principles governing release could be used in the design/optimization of this class of delivery vectors Release of Cu ... competing ligand-dependent effects on the rate of Cu release (see Discussion) In vitro toxicity of CuCNPs in HeLa cells The in vitro toxicity of CuCNPs in HeLa cells (a cervical adenocarcinoma) was...
- 10
- 495
- 0
Báo cáo hóa học: " Novel Strategy to Fabricate PLA/Au Nanocomposites as an Efficient Drug Carrier for Human Leukemia Cells in Vitro" pdf
... doxorubicin was contained in the culture to maintain K562/AO2 cells’ drug resistance in its daily culture The inhibition of cell growth was measured by MTT (Microculture Tetrazolium) assay Initially, ... electrostatic interaction and other non-covalent binding Since the PLA nanofiber has a very high continuous surface area, it has attracted a great deal of attention in fabricating continuous ultrafine ... prepared according to the previous report [30], in which 100 mL of 0.01% HAuCl4 was heated to boiling with uninterruptedly agitating and 3.0 mL of 1% trisodium citrate was dropped into the above...
- 8
- 336
- 0
Báo cáo y học: "Plasmatic B-Type Natriuretic Peptide and C-Reactive Protein in Hyperacute Stroke as Markers of Ct-Evidence of Brain Edema." pptx
... role of ANP in regulating blood flow in the infarcted area was studied in stroke patients and a statistically significant increase in the number of ANP-immunoreactive astrocytes was found in the ... was unavailable for us In conclusion, our work adds something new to the understanding of the elevation of CRP and NT-PBNP in the acute phase of stroke both CRP and NT-PBNP are markers of brain ... NT-PBNP of three determinations for all of the patients of the study These values were increased in the three determinations in comparison to those of healthy people with the highest peak in the...
- 6
- 354
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25