trachomatis directed pcr and lcr testing for infected monocytes as a function of template preparation method
Báo cáo y học: "Detection of Chlamydia trachomatis-DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems" pps
... parts of DNA extraction as well as parts of PCR analysis Additionally IB performed transportation of samples HZ and JK conceived of the study and participated in its design and coordination and ... methods as described below DNA preparation methods Total DNA was prepared from SF spiked with C trachomatis EB and C trachomatis PBMO by each of five different methods; μl of each DNA preparation ... responsible for organizational aspects of the study, collection of clinical samples, culture of C trachomatis and performed DNA extraction, PCR analysis and drafted the manuscript IB and SM performed parts...
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Euro English A Debate and its Implications for Teaching English as a Foreign Language
... Euro-English Part two tests the acceptability of ten features that have been claimed to be specific for mainland Europeans Results of this study reveal that there is little awareness of non-native varieties ... of standard norms, many comments indicate that teachers think about issues of identity and how their teaching could be affected by a broader scope that exceeds traditional methods i TABLE OF CONTENTS ... 77 Awareness of other Varieties of English 78
Awareness of Euro-English as a Topic of Discussion 80
Student Age Groups and Motivational Levels 83
iii CHAPTER page Varieties of...
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Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt
... pH range from 5.3 to Tsuruga and Shikama [21] confirmed that the fast phase of oxidation was due to the a chains and the slow phase was due to the b chains Tsuruga et al found that the beta chain ... of pH on the auto-oxidation of Hb A0 has been the subject of several studies Mansouri and Winterhalter [5] reported that the oxidation of the a chains of Hb A0 was 10 times faster than that of ... initial fast phase of the reaction, but decreased the rate of the slow phase of oxidation in the presence of EDTA A comparison of rough and smooth LPSs of E coli and S minnesota in the presence of...
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Báo cáo y học: "A general framework for quantifying the effects of DNA repair inhibitors on radiation sensitivity as a function of dose" pptx
... observation that δz was a significant parameter in all cases, and that the magnitude and direction of this effect varied according to cell line and confluence, suggests that this variable is an ... flasks was calculated in the usual way For drug-treated flasks, surviving fraction was calculated using the mean, unirradiated, drug-free plating efficiency as the denominator This method revealed ... drug-treated case; and δx – where "x" is any of the parameters above – is the variation on x between the control and case under study General least square fitting was used and the significance of terms...
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Báo cáo y học: "Gene expression response in target organ and whole blood varies as a function of target organ injury phenotype" ppsx
... triglycerides In addition, an enzymatic activity for the following proteins was measured: alanine aminotransferase, alkaline phosphatase, aspartate aminotransferase, lactate dehydrogenase, and sorbitol ... USA) and serum was separated Clinical chemistry analysis was performed on all rats using a COBAS MIRA (Roche Diagnostics, Montclair, NJ, USA) using commercially available reagents from Equal ... The amount and quality of the cRNA was assessed using a NanoDrop ND-1000 spectrophotometer and an Agilent Bioanalyzer The cRNA was then fragmented and hybridized to the Rat Genome 230 2.0 Array...
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top-antitop cross section measurement as a function of the jet multiplicity in the final state and beyond the standard model top-antitop resonances search at the atlas detector at cern
... resonance decaying in a top-antitop pair, using ATLAS data at center -of- mass √ energy of s = TeV The latter analysis is repeated for ATLAS data col√ lected with s = TeV Performance studies of b-tagging ... described by a Lagrangian density that can be separated into a gauge term, a matter term, a Yukawa term and a Higgs term [30]: LSM = LMatter + LGauge + LYukawa + LHiggs The matter Lagrangian contains ... models, so that a comparison of the measurements and searches in data and simulation can be used to test proposed extensions of the Standard Model A comparison of the data and the simulated result...
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A study on the mutual coupling effects between 2 rectangular patch antennas as a function of their separation and angles of elevation
... battle field to commercial enterprises, the microstrip antenna is fast replacing many conventional antennas The advantages and disadvantages of the Microstrip Antenna are tabulated below: Advantages ... theory and experimental results was close especially when the assumptions used in our formulation were adhered to iv List of Tables Table 1.1: The Advantages and Disadvantages of Microstrip Antennas ... Antennas It is actually the last advantage in the list above that makes microstrip antennas so popular today Many characteristics of a single microstrip patch antenna can be modified and engineered...
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Cardiovascular responses as a function of ethnicity, gender, 5HTTLPR genotype, dispositional anger and negative affect
... genotype, and negative affect on the cardiovascular responses of males and females of Indian, Chinese and Malay ethnicity 1) Trait anger and habitual anger expression styles potentially initiate negative ... Indian males in Singapore had an average heart disease mortality rate that was 1.81 times higher than Malay males and 2.69 times higher than Chinese males (Registry of Births and Deaths, 1991) Malay ... Chinese, Indians and Malays For Indians, cardiac output was a positive function of hostility whereas vascular resistance was negatively associated with hostility, suggesting that Indians high in...
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báo cáo hóa học:" Validation of a HLA-A2 tetramer flow cytometric method, IFNgamma real time RT-PCR, and IFNgamma ELISPOT for detection of immunologic response to gp100 and MelanA/MART-1 in melanoma patients" doc
... PBMC was evaluated Calibration standard curve and linearity of dilution Due to the lack of a standard reference material, calibration standard curves were not evaluated for quantification of cellular ... one patient, as an example, was detected by all three validated clinical assays, HLA -A2 MART-1 tetramer assay, IFNγ real time RT -PCR and ELISPOT (Figure and Table 10), demonstrating the assay utility ... Accuracy and precision The real time RT -PCR assay was examined for assay accuracy and precision by spiking 1000 copies of IFNγ plasmid per sample in 80 repeats (n = 80) for intra-assay and 18...
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Báo cáo sinh học: " Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" pptx
... HA and quantitative real-time PCR data employed for the determination of JC viral load A – C: Analysis of HA and quantitative real-time PCR data employed for the determination of JC viral load ... between real-time PCR and HA assays for the determination of JC viral load JCV(Mad1) was propagated in primary human fetal glial (PHFG) cells and purified in Dr Duard Walker's laboratory (I) ... agglutination and traditionally, hemagglutination (HA) and HA inhibition (HAI) assays have been employed for quantitation of JCV [8-14] However, the HA assay is poorly sensitive and the in vitro quantitation...
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báo cáo hóa học:"Comparison of real-time PCR and hemagglutination assay for quantitation of human polyomavirus JC" potx
... HA and quantitative real-time PCR data employed for the determination of JC viral load A – C: Analysis of HA and quantitative real-time PCR data employed for the determination of JC viral load ... between real-time PCR and HA assays for the determination of JC viral load JCV(Mad1) was propagated in primary human fetal glial (PHFG) cells and purified in Dr Duard Walker's laboratory (I) ... agglutination and traditionally, hemagglutination (HA) and HA inhibition (HAI) assays have been employed for quantitation of JCV [8-14] However, the HA assay is poorly sensitive and the in vitro quantitation...
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Báo cáo khoa học: "Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes" ppt
... low as ng of total RNAs extracted from dormant potato tubers or as low as 0.1 pg of PVM RNA was readily detectable by real-time quantitative RT -PCR (data not shown) Analysis of PCR amplicons based ... RT -PCR followed by RFLP analysis for confirmation ELISA Potato tubers and leaves as well as indicator plants were screened for the presence of PVM by a standard double antibody sandwich ELISA ... identified and characterized, on the basis of bioassay and serological reactions (Table 1) All isolates were maintained in infected potato plants that were grown under greenhouse conditions and progeny...
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Báo cáo y học: "DetectiV: visualization, normalization and significance testing for pathogen-detection microarray data" pot
... visualization, normalization and significance testing of pathogen detection microarray data R is a freely available statistical software package available for Windows, Unix/ Linux and MacOS, meaning ... as a package for R, a powerful, opensource software package for statistical programming [10] Many packages for R already exist for the analysis of biological datasets, including microarray data, ... published software on a publicly available microarray dataset DetectiV is available as a package for R, a platform-independent statistical software package, and requires little configuration or...
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The welfare state as a determinant of women’s health: support for women’s quality of life in Canada and four comparison nations pptx
... Canada and four comparison nations, 2001 Canada Overall rank Seats in parliament (%) Female legislators, senior of cials, managers (%) Female professional and technical workers (%) Ratio female:male ... and US and to a lesser extent in Canada 9.1 Decline of the welfare state Teeple [11] sees increasing income and wealth inequalities and the weakening of social infrastructures within Canada and ... indicator of general quality of life and women’s quality of life as compared to the UK and the US Canada does not perform well in relation to the values on a number of indicators of Denmark and...
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Báo cáo khoa học: Combining theoretical analysis and experimental data generation reveals IRF9 as a crucial factor for accelerating interferon a-induced early antiviral signalling pdf
... concentrations result in earlier maximal pathway activation and an increase in signal amplitude [3,4] Furthermore, alterations in the activity of a kinase or phosphatase may affect the speed of signalling ... using an RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) The RNA was used for the quantitative real-time PCR and microarray analysis To generate cDNA, lg of total RNA was transcribed using a QuantiTect ... initial and final concentration, as well as the time and height of the peak for each simulated species, had to fit into a ±20% threshold of the measured data Of 10 000 evaluated parameter sets, approximately...
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Báo cáo khoa học: "A Part of Speech Estimation Method for Japanese Unknown Words using a Statistical Model of Morphology and Context" pptx
... kanji katakana katakana-kanji kanji-hiragana hiragana kanji-katakana kat akana-symbol-katakana number kanji-hiragana-kanji alphabet kanji-hir agana-kanji-hir agana hiragana-kanji percent 45.1% 11.4% ... Table shows examples of common charto Chinese characters Hiragana and katakana are acter bigrams for each part of speech in the infresyllabaries: The former is used primarily for grammatical function ... < a l p h a > , < h i r a > , < k a t a > , and < k a n > represent a sequence of symbols, numbers, alphabets, hiraganas, katakanas, and kanjis, respectively < k a n - h i r a > and < h i r a...
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A Study on the Effects of Argentine Tango as a Form of Partnered Dance for those with Parkinson Disease and the Healthy Elderly pptx
... those at risk of heart failure found that the waltz was just as good as traditional aerobic exercise and that people were happier, which was demonstrated by increases in a measure of quality of ... for their assistance with this study This work was supported by a grant from the Marian Chace Foundation to Madeleine Hackney and a grant from the American Parkinson Disease Association to Gammon ... the task: Interference effects of functional tasks on walking in Parkinson’s disease and the roles of cognition, depression, fatigue, and balance Archives of Physical Medicine and Rehabilitation,...
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Chapter 2Communicating Over the Network Quangkien@gmail.com.OverviewDescribe the structure of a network, including the devices and media that are necessary for successful communications. Explain the function of protocols in network communications. Ex potx
... be installed – The amount of data and the speed at which it must be transmitted – The cost of the media and installation 17 Local Area Network (LAN) Local Area Network (LAN) An individual network ... Frame Header IP Header Data App TCP Header Header Frame Trailer Data Message: Data Multiple protocols 26 Multiple protocols (encapsulated) HTTP Header Protocols Frame Header IP Header Data App TCP ... Data App TCP Header Header Frame Trailer Data Encapsulation – Process of adding a header to the data or any previous set of headers Decapsulation – Process of removing a header 27 Example: Protocol...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25