the profile of cellular high ros changes in human bladder cancer cell lines after live bcg treatment
Mycobacterium bovis, BCG modulation of gene expression in bladder cancer
... The profile of cellular High ROS changes in human bladder cancer cell lines after lyo BCG treatment 91 Table 4.3b The profile of cellular High ROS changes in human bladder cancer cell lines after ... production in human bladder cancer cell lines ………………………… 85 Introduction……………………………………………………………………… 86 4.1 Integrin expression and BCG internalization profiles of human bladder cancer cell lines ……………………………………………………………………………… ... Table 4.1 Integrins α5 and β1 expression and live BCG internalization profiles of human bladder cancer cell lines 87 Table 4.2 Effects of blocking mammalian protein synthesis on BCG internalization...
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Tài liệu Báo cáo Y học: Characterization of an omega-class glutathione S-transferase from Schistosoma mansoni with glutaredoxin-like dehydroascorbate reductase and thiol transferase activities pptx
... glutathione In all cases: Lane 1, protein used in the binding step Lane 2, supernatant of the binding-step Lane 3, eluted fraction Compound used to elute in each case are indicated in the figure ... of SmGSTO observed in S mansoni parasitic life stages rather than in free-living life stages, suggesting that this protein may play a role in the survival of the parasite within the host An increased ... with accuracy The program was unable to model the C-terminal domain of SmGSTO due to the low rate of identity between the two proteins in this region However, as the active sites of the GSTO1-1...
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Báo cáo khoa học: Vaccination with prion peptide-displaying papillomavirus-like particles induces autoantibodies to normal prion protein that interfere with pathologic prion protein production in infected cells pptx
... peptide of nine amino acid residues, DWEDRYYRE, of the murine ⁄ rat prion protein (amino acids 144–152) was incorporated into an immunogenic surface loop of the L1 major capsid protein of bovine ... of PrPSc de novo synthesis The mouse neuroblastoma cell lines N2a (ATCC CCL 131) and prion-infected ScN2a have been described previously [43] Cells were maintained in Opti-MEM medium containing ... period to allow PrPSc synthesis, either in the presence or the absence of immune sera as indicated at a dilution of : 50 As a control for the inhibition of de novo synthesis, the mAb 4H11 (10 lgÆmL)1)...
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Báo cáo khoa học: Purification and cloning of a Delta class glutathione S-transferase displaying high peroxidase activity isolated from the German cockroach Blattella germanica pptx
... provide further information on the in vivo allergenicity of BgGSTD1 In vitro ELISA conducted using lg of protein showed that BgGSTD1 elicited 70% of the IgE-binding activity of the recombinant Bla ... (< 10%) of the GST activity was detected in the flowthrough fraction Further experiments confirmed that the lack of binding was not due to overloading of the column matrix SDS ⁄ PAGE of the affinity-purified ... six classes of cytosolic GSTs in insects [2] The majority of GSTs are in the Delta and Epsilon classes, and the remaining enzymes are in the Omega, Sigma, Theta and Zeta classes The German cockroach...
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Báo cáo khoa học: Transfection with 4-hydroxynonenal-metabolizing glutathione S-transferase isozymes leads to phenotypic transformation and immortalization of adherent cells pdf
... (cytoplasmic injections, with OG-dextran as the marker of the injected cells) or sample containing DNA (nuclear injections, with GFP as the marker of the injected cells), using the electronically interfaced ... h after the injection, any cells killed by the injection procedure were lifted off the dish leaving only the injected cells that survived the injection Such cells were flat and attached to the ... were injected, to allow easy localization of the injected cells Single -cell assay of post-injection viability and GSinduced cell rounding or apoptosis Fluorescence microscopy (IX70 inverted microscope)...
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Báo cáo khoa học: Purification and partial characterization of seven glutathione S -transferase isoforms from the clam Ruditapes decussatus pptx
... Kunze [20] for the purification of GSTs from porcine liver The originality of the method used in the present study is the combination of two elution concentrations and the use of two affinity columns ... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 16 Laemmli, U.K (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage Nature ... allows one of the isoforms to catalyze the conjugation in optimal conditions, whatever the concentration of endogenous GSH This phenomenon is of great interest as GSH is involved in many cellular...
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Báo cáo Y học: Selection of effective antisense oligodeoxynucleotides with a green fluorescent protein-based assay Discovery of selective and potent inhibitors of glutathione S-transferase Mu expression doc
... EGFPcontaining proteins were visualized with enhanced chemiluminescence The positions and molecular weights of the marker proteins are indicated in the left margin In the right margin, the estimated ... c The number of mismatches in the corresponding region of the nontargeted isoform are given Mismatches are underlined in the sequence Determination of the concentration/activity profile of the ... totally ineffective in inhibiting the expression of either of the EGFP-containing proteins in our study This may be attributed to the fact that the ODN was a phosphorothioate-capped PO-ODN The sensitivity...
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Báo cáo y học: "4-Hydroxynonenal induces apoptosis in human osteoarthritic chondrocytes: the protective role of glutathione-S-transferase" doc
... Pre -treatment of cells with anti-Fas/CD95 or PARP inhibitor partially reduced cell mortality, suggesting a role for Fas/CD95 and PARP in HNE-inducing cell death Second, in investigating the classi- ... subsequently followed by the activation of JNKs, caspase 3, and the onset of apoptosis in human lens epithelial cells [29] However, the induction of p53 by HNE remains unclear In RAW 264.7 cells, Haynes ... for increasing times of incubation in the presence or absence of 200 μM NAC (Sigma-Aldrich, Oakville, ON, Canada) To explore the signalling cascade in HNE-induced cell death, cells were incubated...
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Báo cáo y học: " Genetic polymorphisms in glutathione S-transferase (GST) superfamily and risk of arsenic-induced urothelial carcinoma in residents of southwestern Taiwan" ppt
... μg/L Cancer Incidence Individuals’ unique national identification was used to link with the national cancer registry profile in Taiwan to identify the cancer status of each cohort subject The cancer ... statistical software (version 9.1.2, SAS Institute Inc., Cary, NC, USA) The study was approved by the ethics board of the institution prior to starting the study and the informed consent was obtained ... largely confined to the individuals with cumulative arsenic exposure higher than 20 mg/L*years in the present study On the other hand, the association of GSTT1 non-null with increased bladder cancer...
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Báo cáo y học: " Glutathione S-transferase omega in the lung and sputum supernatants of COPD patients" potx
... understanding about the antioxidant defence in human lung and extracellular fluids The important new finding of the presence of GSTO1-1 in extracellular fluids will require further investigation ... several other markers of intracellular proteins including β-actin Over 80% of the cells were viable which also argues against any leakage of GSTO1-1 through damaged cell membranes It is therefore ... received N-acetylcysteine treatment Sputum was induced by inhalation of 4.5% hypertonic saline given at 5-minute intervals for a maximum of 20 minutes according to the guidelines of the European Respiratory...
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Báo cáo y học: "Anticitrullinated protein antibody (ACPA) in rheumatoid arthritis: influence of an interaction between HLA-DRB1 shared epitope and a deletion polymorphism in glutathione s-transferase in a cross-sectional study" ppt
... evidence of interaction in one of the two other cohorts examined In these two other cohorts, but not in SONORA, ever smoking showed a borderline association with ACPA positivity with ORs approaching ... strata defined by the risk factors of interest [35] Evidence of multiplicative interaction was examined by modeling the product term of interest To optimize study power, assessments of interaction ... encoding a conserved amino acid sequence in the third hypervariable region of the DRB1 chain (commonly referred to as the shared epitope [SE]) [1] Recent efforts have examined the importance of interactions...
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Tài liệu Báo cáo khoa học: Analysis of oxidative events induced by expanded polyglutamine huntingtin exon 1 that are differentially restored by expression of heat shock proteins or treatment with an antioxidant ppt
... analyzed The arrow indicates the position of the more intensively oxidized polypeptide in the assay The bracket underlines the domain (molecular mass range 10–40 kDa) of the oxyblots that contains the ... downstream of the initiator ATG in exon (Ex1) of the 67 exon-containing htt gene [5] Pathogenesis in HD correlates with the cleavage of mutated htt and the release of an N-terminal fragment bearing the ... being analyzed to determine if the increase in fluorescence described above was indeed due to ROS accumulation In the presence of the antioxidant, the fluorescence of httEx1-103QEGFP-expressing cells...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... avin-binding domain of ferredoxin : NADP+ reductase [10] The lysine residue is not conserved; instead, an arginine residue interacts with the FAD molecule of the enzyme In the case of FMN-binding ... of these proteins indicate that the level of positive charge at the phosphate group binding site is considerably higher in both avoredoxin and FeR than in the FMN-binding protein (Fig S5AC) The ... Blanche F (1995) Purication of the two-enzyme system catalyzing the oxidation of the d-proline residue of pristinamycin IIB during the last step of pristinamycin IIA biosynthesis J Bacteriol 177,...
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Analysis of Healthcare Interventions that Change Patient Trajectories doc
... with them Next, we modified the baseline to reflect the presence of the intervention, basing our modifications on the published literature We estimated the effects of the intervention to be the ... at the cost of increased office visits to physicians and an increase in the use of prescription drugs However, there is a potential net savings of several tens of billions of dollars Keeping ... reduction in incidence will result in a reduction in prevalence the number of cases in the population at a point in time Figure S.4 shows the results Again, we assumed that 100 percent of the population...
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Báo cáo khoa học: Epitope analysis of the rat dipeptidyl peptidase IV monoclonal antibody 6A3 that blocks pericellular fibronectin-mediated cancer cell adhesion pot
... 6A3 inhibits the rDPP IV-binding of rat and human breast cancer cells, mouse melanoma cells (Fig 1A) and several other types of cancer cells (data not shown) However, the failure of 6A3 in recognizing ... blocked the inhibition of cancer cell adhesion to DPP IV and soluble DPP IV binding to polyFN-expressing cancer cells (Table 1) Taken together, these data indicate that the 6554 Thr331-dependent linear ... the inhibition of FN ⁄ DPP IV binding The Thr331-dependent linear eight-amino acid region mediates the 6A3-binding in full-length DPP IV Although we identified the 6A3 epitope in a loop area of the...
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báo cáo sinh học:" Community characteristics that attract physicians in Japan: a cross-sectional analysis of community demographic and economic factors" potx
... problem Because the values of most of the variables (including the number of physicians) depend on the size of the population or the size of the area of the municipality, most of the variables ... represents the complete equity in the physician-population distribution The degree of the arc of the Lorenz curve corresponds to the degree of inequity Finally, we calculated the Gini index, which is the ... population of each municipality in ascending order of the physician-to-population ratio We then plotted all the points representing the municipalities onto the plane of coordinates with the X-axis...
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Báo cáo sinh học: "Analysis of the human cytomegalovirus genomic region from UL146 through UL147A reveals sequence hypervariability, genotypic stability, and overlapping transcripts" ppt
... strain in the presence of a wild-type strain was shown to be the result of trans-complementation rather than recombination Similarly, the linkage of the UL146-UL147A ORFs and the stability of individual ... genomic DNA The differences in the sizes of the products reflect differences in the lengths of the intergenic regions These results indicated that there is a single transcript that contains all three ... function and binding and activation of the receptors CXCR1 and CXCR2 [25-27] The variable X residue of the CXC motif in our strains is most often proline, although arginine, threonine, and lysine are...
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Báo cáo hóa học: " Hepatitis C virus NS5A protein binds the SH3 domain of the Fyn tyrosine kinase with high affinity: mutagenic analysis of residues within the SH3 domain that ?" doc
... to the binding energy of the NS5A-SH3 domain interaction To test this hypothesis we constructed the corresponding site-directed mutants of the Fyn SH3 domain in the context of a GSTFynSH3 domain ... that NS5A bound to the SH3 domain of the Fyn tyrosine kinase and that in the context of the intact kinase this interaction led to Fyn activation As Fyn is expressed in Huh7 cells [6] that are ... domain I of NS5A was not involved in interactions with the SH3 domain, however we cannot rule out the possibility that domain I might mediate interactions with the intact kinase Interestingly, the...
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báo cáo hóa học:" High rate of loss to clinical follow up among African HIV-infected patients attending a London clinic: a retrospective analysis of a clinical cohort" ppt
... Linkage with the national database of all HIV patients receiving care in the UK indicated that about half had transferred their care to another clinic in the UK, and the remaining half (20% of all ... advice and insight into the analyses and writing of the paper All authors have had some involvement in the writing of the paper and have read and approved the final manuscript Authors’ information ... clinic visits when they defaulted (usually following initial HIV testing and diagnosis) This highlights the importance of early supportive intervention in the weeks after initial diagnosis in...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25