... were visualized by silver staining (unpublished data) In contrast, only the 70 kDa subunit was observed by South-Western blot analysis (Fig 3A) This is consistent with previous studies showing ... Ku-70 is compromised by the absence of Ku-80 [57] This isthe first report to show that Ku-80 expression is suppressed effectively through cell-line transfection In several studies, it has been ... of stress-responsive proteins For example, overexpression of Ku-70 and Ku-80 or Ku-70 alone specifically inhibits HSP70 expression [56], whereas overexpression of Ku-80 alone suppresses only MT-I...
... Two: English displaces other languages languages, making a single language less necessary 2) As in all evolutionary systems, very successful, dominant species spawn subspecies; English will continue ... privacy.” There is plenty of pessimism, though, about prospects for transparency Some respondents expressed deep concerns about losses of privacy Naturally, many of them are deeply attuned to the issues ... Project Summary of Findings This report presents the views of respondents in two ways First, we cite the aggregate views of those who responded to our survey These answers strike us as most interesting...
... Tom puts on his shoes before he puts on his shirt 380 Taking the train across town is quicker than taking the bus If the first two statements are true, the third statement is a true b false c uncertain ... is Olliopolis located? a the mountains b the coast c in a valley d the desert e the forest The city in the desert got the least rain; the city in the forest got the most rain New Town is in the ... excellent soccer teams The teams are named the Panthers, the Whippets, the Antelopes, the Kangaroos, and the Gazelles The sports reporter, who has just started at the newspaper, has to be careful...
... class Within the AreasDML class, there are separate methods for processing inserts and deletes In each of the method specifications, the datatypes forthe AREAS table s columns are listed: P:\010Comp\Oracle8\521-1\CD\Ventura\book.vp ... and the name of the file must be the same In this case, the class is named AreaOfCircle, so this text is stored in a file called AreaOfCircle.java The class has two methods, called main and area ... variables by using expressions and statements To so, you may use one or more of the fundamental classes provided as part of Java A full listing of those methods is beyond the scope of this book; see...
... depletion studies This validation together with the robustness of the observed consequences for Vpu activities allowed us to conclude that both b-TrCP isoforms are not strictly required for Vpu ... proteins (Figure 1E-G) In this set-up, the provirus-driven GFP expression served as a marker forthe transfection level of individual cells The surface exposure of the stably expressed CD4 was reduced ... addition, we established a real-time PCR-based quantification of the respective endogenous b-TrCP mRNAs This assay allowed us to confirm the isoform-specific interference by the siRNAs and to evaluate...
... extent possible All criticisms and suggestions about revisions, omissions, or inclusion of new material, and requests for assistance with manufacturing problems encountered in the shop are always welcome ... instead of writing out the rule in words; by this means, it is possible to condense, in a very small space, the essentials of long and cumbersome rules The letters used in formulas simply stand ... negative As these numbers must be expressed by the same figures as the positive numbers they are designated by a minus sign placed before them, thus: (−3) A negative number should always be enclosed...
... and many studies did not describe who specifically provided the intervention The final question to be answered was—What arethe costs and possible offsets forthe provision of such services? The ... home care and other supportive services for people with chronic disabling conditions Most sessions of Congress see proposals to expand Medicare coverage forone or more of the services that are ... policy and its statutory limitations Forthe purposes of this report, the committee considered the term “nutrition services” to consist of two tiers The first tier of services is basic nutrition...
... presence of the SV40 large T antigen in these cells enhances their resistance to apoptosis Apoptosis is a tightly regulated mechanism forthe disposal of damaged cells and to remove cells during ... apoptosis in cells, further highlighting the importance of myristoylation for JSP1 functional activity Results JSP1 was myristoylated in cells JSP1 contains the putative myristoylation consensus sequence ... cells (low JSP1 expression), or cells with equally high expression of the myristoylation mutant These results suggest that JSP1 induces apoptosis when overexpressed in cells, and further demonstrate...
... glutting his sight with new cruelties This was the occasion of the first persecution; and the barbarities exercised on the christians were such as even excited the commisseration of the Romans themselves ... was laid upon the christians These persecutions among the christians increased the number of informers and many, forthe sake of gain, swore away the lives of the innocent Another hardship was, ... consumed in the pile, and the request of his friends, who wished to give it christian burial, rejected They nevertheless collected his bones and as much of his remains as possible, and caused them...
... association characteristics The difference in association characteristics of the enzyme from the two sources was surprising because the sequence is extremely well conserved between the two species, ... were constructed by use of an XmnI site When stable transformants expressing these chimeric constructs were studied, it was evident that the sequence difference responsible forthe lack of association ... to the large insertion between the catalytic Asp and His in TPP II This region would, of course, be difficult to model, but as Arg252 is so close to His264, where the structure is conserved, the...
... proteins expressed from these constructs are frequently described in the text and figures as the single mutants Double glycosylation site mutations were generated by using expression constructs forthe ... compare the status of our glycosylation mutants in this assay we observed that the triple mutant displayed a phenotype indistinguishable from the C282Y mutant, with an almost complete loss of the ... or whether there are specific glycosylation sites that are more important than others for ensuring expression and recycling of the protein To test this, we transfected HuTu80 cells to express HFE-N110A–...
... region is seven bases forthe R12 ⁄ D12 substrate (Fig 6B) and one or two bases forthe R9-D9 ⁄ D18 substrate (Fig 6F) These results suggest that the HBD of Tma-RNase HI binds to the downstream ... water molecules, a two-metal ion catalysis mechanism has been proposed for RNase H [14,16,17] According to this mechanism, one metal ion is required for substrate-assisted nucleophile formation and ... binding These results suggest that the HBD of Tma-RNase HI interacts with the substrate in a manner similar to the interaction of the HBD of Hsa-RNase H1 Discussion Importance of HBD for substrate...
... membrane insertase for subunits of cytochrome bc1 oxidase and ATP synthase complexes [9] In this study, we have analysed the biogenesis of MscL using in vivo insertion and assembly assays MscL is an ... protease that is responsible for degradation of unassembled subunits of ATP synthase This indirectly argues that Oxa1 functioning is critical for assembly of the ATP synthase subunits rather than their ... have a significant impact on the level of the MscL–HA complex, suggesting that the Sec translocon is dispensable forthe oligomerization of the MscL subunits Discussion We have analysed the requirements...
... rotor The pellet that comprised the NLPs was resuspended in 300 lL of 10 mm Tris/HCl at pH 8.0 The supernatant with the disks and the low molecular mass forms was retained for gel shift assays SDS/PAGE ... residue is also shared with several other potexviral CP sequences, as seven out of the 18 N-terminal sequences of the potexviruses showed consensus for an F in the position that corresponds to ... PapMV disks and CP6–215 disks differed from those for F13L and F13Y disks, and suggest that there is still some RNA associated with recombinant F13L and F13Y disks This could partially explain the...
... apoptosis; however, HSP70 overexpression significantly prevented such stress-induced apoptosis Because these effects were similar to those of our previous observations forthe same cells undergoing ... stress This is similar to the protective effects of another heat-shock protein, HSP27, against apoptosis, as previously reported [24] The molecular chaperone HSP70 has been shown to inhibit stress-induced ... one- way analysis of variance followed by the Student–Newman–Keuls post-hoc test P < 0.05 was considered statistically significant Acknowledgements This study was supported by the grants from the...
... constructs P128H–pBSSK+ and H128P–pBSSK+, respectively The mutants PigE17D–pBSSK+ and HumanD17E– pBSSK+ were generated using the QuickChange Site-Directed Mutagenesis Kit The constructs PMCPT1STOP–pBSSK+ ... Cloning and expression of the liver and muscle isoforms of ovine carnitine palmitoyltransferase 1: residues within the N-terminus of the muscle isoform influence the kinetic properties of the enzyme ... in the BSSK+ polylinker), resulting in constructs D18PigCPT1B–BSSK+ and D28PigCPT1B– BSSK+, respectively Construction of chimeras P50H, P128H, H50P, H128P for CPT1B expression in P pastoris The...
... traffic at the endosomes and that StxB is able to escape the endosomes to the cytosol Taken together 1588 with the cytoplast results, this suggests that when StxB is trapped within the endosomes, it ... have seen StxB in the ER of the cytoplasts Although it remains possible that such a pathway exists for some endogenous proteins, it is clearly not accessed by StxB Our findings raise the question ... access the ER A fraction of the internalized StxB appeared to escape the endosomes as had occurred in 1586 the cytoplasts This suggested that StxB alone could escape endosomes if it was not sequestered...