telomere length measurement by fluorescent in situ hybridization and flow cytometry

... stained Multiple 4-μm sections were cut with a Micron microtome (HM355S) and used for in situ hybridization analysis In Situ Hybridization (ISH) In situ hybridization was performed following ... KE, TB and KL carried out and supervised the ISH SWS and KE scored the cores KAS, SAS, SA and HS contributed in the clinical and demographic database and KAS and SAS in making the TMAs RB and LTB ... TMAs RB and LTB supervised and participated in the study design, result interpretation and in the writing All authors read and approved the final manuscript Competing interests The authors declare...

Ngày tải lên: 18/06/2014, 16:20

... by in situ hybridization Cytogenet Cell Genet 39, 220-223 Lefevre F & Labonnardi6re C (1986) Molecular cloning and sequencing of a gene incoding biologically active porcine interferon alpha J Interferon ... concerning the washings after hybridization: the slides were first rinsed in x SSC at room temperature, and in 50% formamide and x SSC at 37 °C for lh, then carefully washed in x SSC Labeling was ... washings after hybridization They are less stringent and, in consequence, we have retained signal possibly lost in the first experiments (Yerle et al., 1986) CONCLUSION possibility to obtain...

Ngày tải lên: 14/08/2014, 19:23

... detection of the genus Serpulina, S hyodysenteriae and S pilosicoli in porcine intestines by fluorescent rRNA in situ hybridization Mol Cell Probes 12, 323–330 [20] Brown-Howland, E.B., 1992 Development ... marginale DNA in bovine erythrocytes by slot-blot and in situ hybridization with a PCRmediated digoxigenin-labeled DNA probe J.Vet Diagn Invest 7, 465–472 [47] Gebhart, C.J., 1994 Specific in situ ... filters J Soc Chem Ind 33, 523–539 [13] Arnoldi, J., 1992 Species specific assessment of Mycobacterium leprae in skin biopsies by in situ hybridization and polymerase chain reaction Lab Invest 66, 618–623...

Ngày tải lên: 30/10/2012, 11:02

... longwavelength (350 nm) UV and differentiation by the subsequent staining with Giemsa stain is improved (see Note 1) 3.6.1 Staining for GBG- and RBG-Banding Sensitize slides in a Coplin jar for ... promoter and other regulatory regions of the gene and the chromatin This may be achieved in the future by combining FISH and green -fluorescent protein detection or FISH with nonfluorescent protein ... metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries Hum Genet 80, 224–234 Collins, C., Lin Kuo, W., Segraves, R., Fuscoe, J., Pinkel, D., and Gray,...

Ngày tải lên: 11/04/2014, 09:47

... electrodes by accepting the electrons By substituting the Pt counter electrode with the GO film, the photoinduced electrons could be captured by GO and result in the reduction of GO Inspired by this, in ... carbon and oxygen (C/O), obtained by taking the ratio of C1s and O1s peak areas in XPS spectra, increases from 2.7 to 5.1, which also indicates the reduction of GO did take place during the in- situ ... by an in- situ photoelectrochemical method in a dye-sensitized solar cell assembly Chen Chen1, Mingce Long*1, Min Xia1, Chunhua Zhang1, and Weimin Cai1 School of Environmental Science and Engineering,...

Ngày tải lên: 21/06/2014, 17:20

... 1131 Flow and temperature controller P1 V1 CoCl2 and SmCl3 mixture P2 H1 H2 Inlet V2 Reducing agent and stability Inlet TC1 N2 In N2 In Y-mixer N2 Out Reaction channel N2 Out TC2 N2 Out P3 N2 in ... formation of an ultra-thin oxidation layer caused by including acetone in the quenching solution EDS data for the resulting NPs indicate the elemental oxygen appearing in those NPs (Fig 3d) Analysis ... from New Teacher Funds (2008-00061025) and SRF for ROCS and SEM by the Chinese Education Ministry, and Innovative Research Team of Chinese Education Ministry in University (IRT0512) at Beihang University...

Ngày tải lên: 21/06/2014, 20:20

Ngày tải lên: 28/06/2014, 15:23

... Odile Michel and Xue-Ming Tang, 1997 Biotin and Digoxigenin as Labels for Light and Electron Microscopy in situ Hybridization Probes: Where we stand? The Journal of Histochemistry and Cytochemistry ... virus (GAV) by in situ hybridization during acute and chronic infections of Penaeus monodon and Penaeus esculentus Diseases of Aquatic Organisms 56: – 10 Queenlands Bioscience Precint, 306 Carmody ... (TSV) in Americas: determination by histopathology and in situ hybridization using TSV – specific cDNA probes Aquculture 171: 13 – 26 Anuenue Fisheries Research Center, Department of Land and Natural...

Ngày tải lên: 28/07/2014, 02:21

... mẫu paraffin Đặt mẫu xử lý vào khuôn inox, dùng bình rót paraffin (5 paraffin : sáp ong) vào khuôn chứa mẫu, cố định mẫu cho nằm yên dƣới đáy tách rời Sau đặt khuôn inox chứa mẫu paraffin lên bề ... 21 phân tử lai đƣợc phát cho ligand avidin (hay streptavidin) vào phản ứng Avidin (hay streptavidin) trƣớc có gắn với nhóm phát huỳnh quang hay enzyme xúc tác cho ... dịch Blocking buffer 1X, chất blocking dung dịch khóa phân tử lai không đặc hiệu lại  Ủ mẫu với dung dịch Anti – Digoxigenin (Anti – DIG) có gắn Alkaline phosphatase (AP), Anti – Digoxigenin kết...

Ngày tải lên: 28/07/2014, 02:21

... phƣơng pháp phòng bệnh virus đốm trắng II.5 PHƢƠNG PHÁP IN SITU HYBRIDIZATION II.5.1 Sơ lƣợc phƣơng pháp In situ hybridization Phƣơng pháp In Situ hybridization (ISH) đƣợc phát vào năm 1969 công Gall ... : In Situ hybridization PCR : Polymerase Chain Reaction ELISA : Enzyme Linked Immuno Sorbent Assay RT – PCR : Reverse – transcription polymerase chain reaction PBS : Phosphate Buffered Saline ... 14 II.4.2.5 Phòng trị bệnh 14 II.5 PHƢƠNG PHÁP IN SITU HYBRIDIZATION 14 II.5.1 Sơ lƣợc phƣơng pháp In situ hybridization 14 II.5.2 Khái niệm lai phân tử ...

Ngày tải lên: 28/07/2014, 02:21

... enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled probe, ... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated ... of intracellular viral DNAs in paraffin sections J Vet Med Sci 2003, 64, 231-235 73 Kim O, Chae C In situ hybridization for the detection and localization of porcine epidemic diarrhea virus in...

Ngày tải lên: 07/08/2014, 17:22

... methods used in clinical diagnosis, and certain kits or antibodies are approved by the FDA (U.S Food and Drug Administration) IHC is easy to perform and relatively cheap, and is predominantly used ... results, including variability in tissue fixation and processing, variable sensitivity and specificity of commercially available antibodies, and differences in scoring criteria with considerable interobserver ... variability in interpretation of results [23] Our study is trying to answer a simpler question about the concordance between IHC and FISH in our local setting in Guilin city of China, using some...

Ngày tải lên: 09/08/2014, 04:21

... results also showed that dark staining was observed around the nucleus predominately, indicating that β-actin mRNAs existed mainly in the vicinity of the nucleus In order to analyze the correlation ... insertion with the AFM probes β-actin mRNAs were detected mainly in the vicinity of the nucleus Usually β-actin mRNA is known to exist mainly in the vicinity of the nucleus, and these results agreed with ... performed using µl of RT-PCR reaction buffer as a template The amounts of initial β-actin mRNA were determined by a standard curve built using β-actin cDNA Figure shows some examples of ISH and the...

Ngày tải lên: 11/08/2014, 00:22

Ngày tải lên: 19/12/2014, 20:12

... Fluorescence in- situ hybridization: Fluorescence in- situ hybridization (FISH) is a technology utilizing fluorescently labeled DNA probes (with a fluorescent dye or a hapten, usually in the form ... Histogram showing the percentage of monosomy PAGE X cells and trisomy X cells detected by FISH in control females undetected by the standard G- banding Graph 2: 62 Histogram showing the percentage ... these genes escape inactivation in females to maintain a balance with the situation in males In addition, there are other genes on the X chromosome that are not regulated by X inactivation whose...

Ngày tải lên: 04/10/2015, 15:45

... was also inherited in the transformation from In( OH)3 to In2 O3 successfully Their composition and single-crystalline structures were confirmed using XRD, TEM, and SAED The optical determinations ... caused by the increasing energy gap resulted from decreasing the grain size In order to further understand the optical nature of In2 O3 nanotube bundles, the room PL spectrum was measured and the ... of the In( OH)3 precursor owing to the dehydration of the In( OH)3 precursor in calcination A typically local magnified image of the area marked by the white arrow (Fig 3b) and TEM Results and Discussion...

Ngày tải lên: 22/06/2014, 00:20

... cross-cultural studies appear useful and vital in this way Commenting is common in many languages and cultures It is realized by comforting, showing concern or expressing likes or dislike or reaction, ... giving comments by judges on contestants’ performance after live show in the two latest shows: Vietnam Idol 2010 and American Idol 2011 basing on the politeness theory by Brown and Levinson and ... similarities and differences in the way of giving comments by Vietnamese and American judges are drawn from detailed and critical analysis of data Part C: CONCLUSION Summary of the major findings and...

Ngày tải lên: 10/08/2015, 19:48

... contaminated by noise This thesis addresses both interpolation and approximation by taking principal shiftinvariant (PSI) spaces as the space the fitting function lives in The research topic is inspired ... in one and two dimensions, by choosing the generator φ as a uniform B-spline in 1D and a tensor product of uniform B-splines in 2D Since the iterative process for numerical solution is slow in ... fitting The experiments compare our methods with the classical interpolating and smoothing spline, and the result is that they produce very similar fitting curve or surface in terms of accuracy and...

Ngày tải lên: 11/09/2015, 09:11

... for us I am indebted to many of my colleagues for providing a stimulating and fun environment in which to learn and grow I’m especially grateful to Suey Li, Shue Yin, Lin Ming, Ramesh and Chi Wen ... materials technology and is beginning to impact the new frontier of spintronics Epitaxial Ni/insulator interfaces are of growing interest, and their application concerns many different industrial sectors, ... lattice points11 and found has a larger intersecting volume than texture, thus indicating that lowers the overall misfit and the interfacial energy, in addition to accommodating the...

Ngày tải lên: 09/10/2015, 11:24

... Results and discussion Aniline was obtained from Beijing Xingjin Chemical Factory, and was distilled at a reduced pressure before use DBSA (Anyang Xingya Chem Ltd.) was used as the surfactant and ... X Ding et al / Journal of Colloid and Interface Science 320 (2008) 341–345 by oxidatively polymerizing aniline using ammonium peroxodisulfate as oxidant (NH4 )2 S2 O8 [23] There, the obtained ... producing nanometer-sized particles The PANI nanorods have nearly uniform diameters and lengths by using DBSA Second, magnetite particles were easily formed inside micelle “reactor” during sedimentation...

Ngày tải lên: 20/03/2014, 13:04