... 10 days The data picked up in situ allowed determining through the average and black-box methods, the thermal conductance ofthe wall With this particular type of construction ofthe monitored ... sandstone and calcareous stone of Fasano The goal ofthe laboratory tests was to experimentally find the thermophysical propertiesof different blocks: density, dampness, thermal conductivity, thermal ... other methods of determination of thermal conductance considering the geometric and structural variability ofthe wall section The structure ofthe typical wall of "Trulli" has a geometric and...
... met the DSM-IV diagnostic criteria for ADHD and had a symptom severity of at least 1.5 standard deviations (SD) above norm values for the ADHDRS (ADHD subscale ofthe SNAP in Study 3) The diagnosis ... symptoms using the individual patient data of five clinical trials studying atomoxetine in children and adolescents with ADHD Methods Study design and procedures Individual patient-level data from ... formally diagnosed with ADHD or a hyperactive/inattentive/impulsive syndrome in the past was required for inclusion into the study Another analysis ofthe study data showed that 11. 5% of patients did...
... Src dynamics (A) In the plane of active RPTP and Csk concentrations, bifurcation boundaries separate regions of different types of Src dynamics, determined by the Hopf (red lines) and saddle-node ... states, whereas the dotted black line shows unstable steady states (steady state values are designated as s1SS) Dependence ofthe oscillation amplitude (C) and period (D) on the active Csk concentration ... in the steady-state and dynamic behavior ofthe Src cycle occur In Fig 5, these boundaries are determined by two different bifurcations One is a saddle-node bifurcation where an unstable steady...
... and PPi did not affect the activity when added to the standard assay at or 20 mm Identification ofthe native glucose dehydrogenase in P torridus In order to identify the native GdhA in P torridus ... expressed as lmol of NADPH produced per per mg of protein under the specified conditions NAD+-dependent glucose dehydrogenase activity was measured the same way, substituting NAD+ for NADP+ For determination ... abolished by the chelating agent EDTA However, the addition of Zn2+ did not affect the specific activity ofthe enzyme, and even high concentrations of EDTA (20 mm) could not decrease the activity of...
... reduced form ofthe enzyme For the reduced form, the Soret band can be observed at 415 and 442 nm, and for the oxidized form at 403 and 422 nm The b–band can be seen at 517 nm and the a–band at ... cm)1 and the mode typical for the deprotonated form at 1498 cm)1, indicating the protonation of a tyrosine residue with the reduction ofthe protein Also the m7¢a(CO) and d( COH) of tyrosine side ... refrain from discussing and assigning these modes on the basis ofthe data presented here, in spite ofthe fact that bands in the difference spectra are observed in the region where the modes were...
... and DAHKam-Ni(II) (C), calculated for 0.5 mM concentrations of peptides and metal ions The intensities of CD bands of 4N complexes (constructed by adding intensities at extremes of dd bands and ... coordination (in agreement with previous observations [6,7]) A slight redshift of dd bands accompanied by a subtle decrease of delocalization ofthe unpaired d electron ofthe Cu(II) ion in the DAHKam ... and for related compounds The cases of highest and lowest afnities were selected from literature data The binding afnities for the model peptides are in the middle ofthe range of values for...
... ventricle at the end of systole Preload: The "load" imposed on the ventricle at the end of diastole Measures of preload include end-diastolic volume, end-diastolic pressure and end-diastolic wall ... period of time during which the mechanical propertiesofthe heart are characterized by the EDPVR; 150 knowledge ofthe EDPVR allows one to specify, for the end of 125 diastole, EDP if EDV is ... Technically, it is defined as the reciprocal ofthe slope ofthe EDPVR ([dP/dV]-1) Colloquially, it is frequently used in describing the elevation ofthe EDPVR Stiffness (dV/dP) is the reciprocal of compliance...
... avoredoxin and discuss the key residues for ligand binding and metal ion binding, based on the crystal structures Results Cloning and sequencing ofthe avoredoxin gene We determined the nucleotide ... Resolution ofthe structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side ofthe isoalloxazine ring, and that the 2Â-P-AMP moiety is held in the groove between the ... at the site of either the native or NADP+-bound form; instead this residue is bound to the nicotinamide moiety of NADP+ in the NADP+-bound form These authors also proposed that Cys45 of FeR, which...
... seed preparations increase in ANS fluorescence The increase in the ANS signal was reciprocal to the decrease in monomeric species (data not shown) and depended on the numbers of seeds added (Fig ... ofthe Sup-NM domain [35,36], different fibril structures caused by additional alanines are likely The manner in which the number of alanines determines the atomic structure ofthe fibril and the ... upon addition of seeds Fibrillation in the presence of different concentrations of N-(+7)Ala seeds (seeding) and N-WT seeds (cross-seeding) Rate constants were calculated by assuming a first order...
... protein folds compatible with the SV-IV sequence PHD [33], JPRED [34], and PSIPRED [35] web services were used to predict the secondary structure The 3D model ofthe peptide corresponding to the segment ... peptide at m/z 2081.7 corresponded to the diacetylated peptide 74–90 from the endoproteinase Glu-C digest of acetylated protein SV-IV Signal diagnostics of peptide structure are indicated in the ... alternative, the fold-recognition approach was applied by using three independent servers on the net: 3D- PSSM, GenetTHREAD, TOPITS None ofthe methods identified a known fold suitable for modeling the...
... mediated exclusively on the reduced state ofthe enzyme We hypothesize that this residue imposes a degree of rigidity to the structure ofthe reduced state of class III peroxidases Results and ... to that ofthe wild type, % 11. 1 [18] Finally, comparison ofthe X-ray structures ofthe oxidized forms ofthe native [5,19] and the T171S mutant (protein databank code: 1GW2.pdb) did not reveal ... magnitude ofthe changes in Fe-His band frequencies and the redox potential values This is exemplied by the case of CCP and its mutants D2 35E, D2 35N and D2 35A [27] The H-bond between the proximal...
... level of each candidate index entry Starting with a set of candidate entries, the algorithm automatically decides which entries should be added to the backof -the- book index, based on a set of linguistic ... automatically determine the number of keywords to be extracted, the evaluation of these methods is done under the ratio-based setting, and thus for each method the top 0.45% ranked keywords are extracted ... algorithm The training candidate entries are then labeled as positive or negative, depending on whether the given n-gram was found in the back -of- the- book index associated with thebook Using...
... amount at the 105 110 kDa size The amount of 105 110 kDa protein was reduced and in many instances evidence of aggregation was evident at the top ofthe gels The amount of aggregate present at the ... exchanger and of caveolin, a marker of lipid rafts To avoid possible detergent-induced artifacts that could either alter the distribution ofthe Na+/H+ exchanger or the constituency ofthe lipid rafts, ... transfected with the NHE1 isoform ofthe Na+/H+ exchanger Membranes were fractionated as described in the Materials and methods and separated by SDS/PAGE Membrane fractions for A D and G were prepared...
... Cc of Asp and Cd of Glu, and Og of Tyr Positive unit charges were added at the following atoms: Nt ofthe N-terminus, Nf of Lys, Cf of Arg, and Nd or Ne of His (depending on the position ofthe ... were deduced using the combined information ofthe intensities of Jab couplings from the 2D DQF-COSY spectrum and the NOE intensities ofthe HN-Hb and Ha-Hb cross peaks from the 2D and 3D NOESY ... the structural statistics ofthe ensemble of 24 structures, the lowest energy and the best (smaller rmsd from the mean) structure of RIIa D/ D Each monomer of RIIa D/ D consists of a disordered...
... we do, you do, he does, she does, it does, I did, we did, you did, he did, she did, it did The other extreme is to learn all the rules as taught in those grammar textbooks For the same example,I ... learned nor heard before is a waste of their time and effort If you don’t have the “sound map” yet, you won’t be able to identify the sounds clearly and, therefore, the words If the “sound map” ... placed • the content and topic to which the word or phrase is related • the emotion and/or sense ofthe speaker • the other common words that go along with that word or phrase and the way they...
... observed in the presence of GDPÆAlFx Because ofthe unexpected observation of a monomeric protein species with both GDP and GDPÆAlFx, we tested AlFx binding to the protein by adding 300 lm AlCl3 and ... resulted in a 2.5-fold increase in specific GTPase activity and increased formation of GMP during hydrolysis Thermodynamics and stoichiometry of nucleotide binding We used ITC to investigate the thermodynamics ... Biophysical propertiesof hGBP5a ⁄ b and hGBP5ta M Wehner and C Herrmann hand, interact dynamically with the bound nucleotide, and usually bind nucleotides in the micromolar range In addition to these...
... separated by SDS/PAGE The appearance of a band in the different conditions used (native, reduced, reduced and denatured) on the different proteins (PfPDO and mutants) is evidence ofthe exposure of ... (C35S); (d) PfPDO (C146S) (B) HPLC The disulfide bond-forming activity of PfPDO is monitored using the synthetic decapeptide NRCSQGSCWN and the oxidation of this dithiol peptide to the disulfide state ... ofthe disulfide bridge in the peptide NRCSQGSCWN (A) Spectrophotometric method The disulfide bond-forming activity of PfPDO was monitored using the synthetic decapeptide NRCSQGSCWN Oxidation of...
... and the other at about 43 °C during the thermal-induced unfolding monitored by CD Previous investigations using CD ofthe thermal-induced unfolding of skeletal Tm from other organisms also identified ... DSC curve for WT The solid curve represents the observed data and the dashed curves represent the deconvolution ofthe individual transition into three independent transitions See Table for the ... determined by DSC for the wild type (WT) and mutant Tms The uncertainties listed are the standard errors ofthe mean and included the uncertainty in the determination of protein concentrations The...
... with the binding ofthe 165-Da substrate The time-dependent increase in RU (end point at min) was measured directly from the sensorgrams using the cursor guided reading ofthe X- and Y-coordinates, ... found to be displaced by 2.6 A (mean distance) in the direction ofthe iron and Glu286 upon substrate binding, and in addition, the hydrogen-bonding network for the cofactor was slightly different ... [18] or reduced [20] pterin cofactor onto the structure ofthe ligandfree rPAH (containing the regulatory and catalytic domains) [19] revealed that both the reduced and the oxidized cofactor interact...
... gene in E coli, and the production of active TPS in these cells Thepropertiesofthe recombinant enzyme have been determined, and are compared to thepropertiesofthe TPS purified from M smegmatis ... measured by the anthrone assay (colorimetric), and was compared to the amount of NAD+ produced (nmol) in the coupled assay, as an indirect measure ofthe amount of trehalose-P synthesized However, ... separated from maltose (M) and glucose (G) shock Disruption of this gene did impair development of hyphae and did decrease the infectivity ofthe organism, but it was not lethal Thus, the rate of...