structure function and role in human pathogenesis
Tài liệu Báo cáo khoa học: Role of cleavage and shedding in human thyrotropin receptor function and trafficking pdf
... folding, trafficking and ligand-mediated signaling but not for ligand binding [37,40] The complete loss in the constitutive activity of the Ó FEBS 2003 Role of cleavage and shedding in TSHR function ... measuring the binding of [125I]Streptavidin Specific binding at each point was normalized with reference to specific binding before the incubation at 37 °C (0-min point) to derive the fraction of initial ... domain and devoid of any tag was not functional [27], while a mutant containing four amino acids of the ectodomain and including a rhodopsine tag was found constitutively activated [29] In agreement...
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Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx
... changes in the cytoskeleton that may occur during the initial h window of infection We infected RPE cells with K26GFP [27], and stained cells for F-actin, using phalloidin at 30 and h postinfection, ... to fully understand HSV-1 infection of all target cells, including RPE cells, our study provides new starting points for understanding viral pathogenesis in the retina and advancing novel therapies ... boxed regions in (b) and (e) are highlighted in (c) and (f) Arrows and arrowheads in (c) and (f) indicate the association of HSV-1 GFP particles with actin-stained rhodamine phalloidin (g, h) Effect...
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Tài liệu Báo cáo khoa học: The small heat shock proteins and their role in human disease pptx
... opaque with age and contain many inclusion bodies reactive with antibody to aB-crystallin, but not to b- and c-crystallin, suggesting an important role for aA-crystallin in maintaining lens transparency ... paralysis, and the proteins colocalize intracellularly, suggesting a role for molecular chaperones in Ab toxicity and metabolism Human recombinant aB-crystallin also interacts with Ab in vitro, and ... formation of huntingtin-containing inclusion bodies in Huntington’s disease encourages cell survival, whereas monomers and small inclusion bodies of huntingtin, a protein possessing abnormal polyQ...
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Báo cáo khoa học: Effect of valine 106 on structure–function relation of cytosolic human thymidine kinase Kinetic properties and oligomerization pattern of nine substitution mutants of V106 ppt
... Mg2+-binding motif VIGID97 [19,20] and the putative thymidine-binding motif FQRK131 [20] are in italics in all sequences In the human sequence, the b-branching amino acids and the a-helix breaking ... the structure and function of the enzyme by mutating Val106 to amino acids differing in polarity, size and conformation, and subsequently investigating their effect on the quaternary structure and ... DNA synthesis in mouse 1-cells Effect of 5-fluorodeoxyuridine and methotrexate in thymidine kinase plus and thymidine kinase minus cells J Biol Chem 251, 2938– 2944 Pontarin, G., Gallinaro, L., Ferraro,...
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Báo cáo y học: "Dipeptidyl peptidase IV activity and/or structure homologs: Contributing factors in the pathogenesis of rheumatoid arthritis" doc
... expression and the rapid upregulation and sustained signal following activation supports a role for CD26 in maintaining and regulating stimulation Although crosslinking of CD26 with CD3 leads to increased ... of cell function and/ or release of indicated mediator ‘Endogenous opioids’ include enkephalins, endorphins, dynorphin and endomorphin CGRP, calcitonin gene-related peptide; IL, interleukin; NPY, ... cell function and/ or release of indicated mediator CCR, CC receptor; IL, interleukin; IP-10, IFN-γ inducible protein-10; Mig, monokine induced by interferon-γ; MIP, macrophage inflammatory protein;...
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Báo cáo y học: " Th2 cytokines and asthma Interleukin-4: its role in the pathogenesis of asthma, and targeting it for asthma treatment with interleukin-4 receptor antagonists" pot
... those involved in insulin signaling, the insulin receptor substrate (IRS)-1, IRS2 and signal transducer and activator of transcription (Stat)-6 [23] IRS1 and IRS2 regulate proliferation and protection ... Acting as a decoy to bind and neutralize circulating cytokine, coupled with the high specificity and high affinity of binding for the cytokine, makes the soluble receptor ideal as a cytokine ... arthritis in adults and children Clinical studies with rhuIL-4R (Nuvance™) The promising results in preclinical studies led to preliminary investigations in which rhuIL-4R proved safe and effective in...
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HUMAN CARBOXYLESTERASE 2 SPLICE VARIANTS: EXPRESSION, ACTIVITY, AND ROLE IN THE METABOLISM OF IRINOTECAN AND CAPECITABINE
... Jugovic; and my cousins Erin Dunivan and Kristin and Scott Petherick Your love and support throughout my life and education has been and continues to be incredible My brother Robbie Collins for ... challenging me and supporting me in ways only a sibling could I am grateful that you are both my brother and my friend My parents Bob and Mary Ann Collins for always loving me, supporting me and inspiring ... carboxylesterases include angiotensin-converting enzyme inhibitors, salicylates, haloperidol, cocaine, heroin, and the chemotherapeutics irinotecan and capecitabine (Satoh and Hosokawa, 1998)...
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Báo cáo y học: " Vgf is a novel biomarker associated with muscle weakness in amyotrophic lateral sclerosis (ALS), with a potential role in disease pathogenesis"
... C-terminal epitope of Vgf Unoccupied binding sites on the plates were blocked by incubation with casein Samples and standards were applied in duplicate and incubated overnight at 4°C Following ... sequencing, and the recombinant virus was packaged by infecting the PacI linearized recombinant viral DNA into human embryonic kidney (HEK)-293 cells (Clontech, CA) The resulting recombinant virus ... delimiting the cellular area of interest free hand, using predetermined criteria to define the region of interest The immune intensity of the cellular Vgf encompassing the L3-L5 regions of spinal...
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Tài liệu Báo cáo khoa học: Relationships between structure, function and stability for pyridoxal 5¢-phosphate-dependent starch phosphorylase from Corynebacterium callunaeas revealed by reversible cofactor dissociation studies doc
... steps involved in subunit dissociation and reassociation will contribute to a better understanding of the dimerization process per se and the role of interprotomeric contacts to generate a functional ... Enzymeoxyanion interactions occur at a protein site different from the active site, and thermostabilization is the result of a protein conformational change induced by the binding event Residues involved in ... However, the characteristic minima in ellipticity at 208 nm and 222 nm have greater intensities in the native enzyme, suggesting partial loss of a-helical structure in apo-CcStP and reconstituted holo-CcStP...
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The Fungal Community Its Organization and Role in the Ecosystem Third Edition docx
... level? The in uence of scale and heterogeneity in the ecosystem is a confounding factor in our attempts to understand ecology and community functioning However, these factors are inherent in ecosystems, ... play essential roles in nearly every aspect of ecosystem development, stability, and function Fungi and fungal community structure play major roles in determining both above- and belowground ... organization and structure of terrestrial systems Exploring the interactions of fungi within the fungal community and their role in determining plant communities, especially as decomposers and mutualists,...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... antinociceptive, neuroprotective and anti -in ammatory properties in different experimental models [30–35] Ebselen inhibits leukocyte in ltration and activation, leading to elimination of H2O2 in ... cytotoxicity against some human cell lines, such as human keratinocytes (HaCaT cells), human embryo lung fibroblast cells (HEL cells), human nasopharyngeal carcinoma cells (HEp-2 cells) and PMNs in vitro ... selenocysteine residue at the active site [20–22] It catalyzes the reduction of H2O2, and a variety of organic hydroperoxides, resulting in effective elimination of various ROS in vivo and in vitro...
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Báo cáo khoa học: K4, K9 and K18 in human histone H3 are targets for biotinylation by biotinidase pdf
... biotinylated lysines in histone H3: N1)9 (including K4 and K9), N9)16 (including K9 and K14), N16)23 (including K18 and K23), and N18)25 (including K18 and K23); subscripts denote the amino acid ... alanine (peptide R8A6)13) biotinylation increased considerably, suggesting that unmodified arginines interfere with biotinylation of lysines by biotinidase Substitution of arginine with ornithine ... stained using DAPI, and the cytoplasm was stained using rhodamine phalloidin Images entitled ‘Merged’ were created by overlaying images obtained by staining with antibody, DAPI and rhodamine...
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Báo cáo khóa học: Ouabain stimulates endothelin release and expression in human endothelial cells without inhibiting the sodium pump Robert Saunders and Georgios Scheiner-Bobis pptx
... liquid-scintillation counting The 86Rb+ activity was normalized against the amount of protein [13] per well 86 Rb+ uptake was also investigated after preincubating the cells for 15 with lM protein kinase ... were interested in determining whether ouabain added to endothelial cells in culture might induce such a response Various concentrations of ouabain were added to the cell culture wells, and, ... ouabain exposure was investigated by Western blotting using an antibody against the phosphorylated form of p44/p42 MAP kinase Using this method, MAP kinase activation was clearly detectable in...
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Báo cáo khoa học: Serpins 2005 – fun between theb-sheets Meeting report based upon presentations made at the 4th Interna-tional Symposium on Serpin Structure, Function and Biology (Cairns, Australia) doc
... molecular insight into how serpins can survive in an extreme environment There were many new insights into the structure and biochemistry of serpins with extra-inhibitory and cross-class inhibitory functions ... providing an interesting counterpart to the role of maspin in embryogenesis [29] A large proportion of the meeting was devoted to understanding and controlling inappropriate conformational change in ... cathepsin inhibitory activity and MENT positioning on chromatin One of the major questions in the field of serpin biology is the precise role and mechanism of function of three noninhibitory human...
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báo cáo sinh học:" Continuity and change in human resources policies for health: lessons from Brazil" docx
... attracting and retaining health staff in remote and/ or rural areas, tackling staff mal-distribution and over-specialisation, particularly in the physician workforce, retaining and motivating health ... respond to primary care demands within SUS PROFAE 2002/2007 Aimed at expanding training of nurse technicians and nursing aides PROMED 2003/ Aimed at financing curricular reform in medical schools directed ... training curriculum: there was an increased emphasis on rural internships and the need to provide trained staff in underserved areas In 1977, the first mandatory rural internship was created in...
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Báo cáo sinh học: "Multiplex Zymography Captures Stage-specific Activity Profiles of Cathepsins K, L, and S in Human Breast, Lung, and Cervical Cancer" potx
... All human recombinant cathepsins were from Enzo Life Sciences (Plymouth Meeting, PA) Human cathepsins K and S were expressed in insect cells, human cathepsin V was expressed in NSO cells, and human ... Cathepsins K, L, and S in Human Breast, Lung, and Cervical Cancer Binbin Chen and Manu O Platt* Abstract Background: Cathepsins K, L, and S are cysteine proteases upregulated in cancer and proteolyze ... breast tissue protein was determined by loading increasing doses of recombinant cathepsins K and L in the same gel as the breast cancer and normal specimens to generate a standard curve to which...
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báo cáo hóa học:" Augmented inhibition of angiogenesis by combination of HER2 antibody chA21 and trastuzumab in human ovarian carcinoma xenograft" pptx
... substrates and activates a variety of signaling cascades, including the phosphatidylinositol-3 kinase (PI3K)/serine/threoninespecific protein kinase (AKT), and it regulates various cell functions ... especially in tumor growth, and angiogenesis [15] In a previous study, we had found chA21 monotherapy could inhibit human ovarian carcinoma cell line SKOV-3 growth in vitro and in vivo [16] In this ... potency on inhibiting the AKT It may partly explain our findings that the combination of chA21 with trastuzumab could synergistically enhance the in vitro and in vivo anti-angiogenesis effects In conclusion,...
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Báo cáo y học: "Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration" ppt
... block of tissue (incorporating AF and NP in continuity) was fixed in 10% neutral buffered formalin and processed to paraffin wax Sections were taken for haematoxylin and eosin staining to score the ... senescence in ageing and agerelated pathologies Indeed, Roberts and colleagues [12] and Gruber and colleagues [13] have shown increased staining for senescence-associated β-galactosidase (SA-β-gal) in ... Jayson MI: Age related changes in the structures within and bordering the intervertebral foramen: associations with low back pain In The Ageing Spine Edited by: Hukins DW, Nelson MA Manchester:...
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