standard protocol for in situ hybridization

... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated ... hybridization protocol resulted in the increasing sensitivity as compared with hourshybridization protocol Digoxigenin-labeled probe was capable of detecting CHV in MDCK cells infected with 103 TCID50 using ... enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin fixed and paraffin embedded CHV-infected cells The optimum result was obtained using digoxigenin-labeled probe,...

Ngày tải lên: 07/08/2014, 17:22

... Traditional Chinese Medicine Hospital which are the two main hospitals for the treatment of breast cancer in Guilin city of China The specimens were fixed in 10% neutral-buffered formalin (pH7.4) for 24 ... probe The interpretation of IHC and FISH were each performed by investigators blinded to the results of the other assay IHC analysis IHC study was performed on paraffinem-bedded, formalin-fixed ... visualization reagent for 30 minutes each, and finally with a 3,3'-diaminobenzidine chromogen solution After a finally wash, the slides were counterstained with haematoxylin [15] Tumor cells with...

Ngày tải lên: 09/08/2014, 04:21

... DNA in bovine erythrocytes by slot-blot and in situ hybridization with a PCRmediated digoxigenin-labeled DNA probe J.Vet Diagn Invest 7, 465–472 [47] Gebhart, C.J., 1994 Specific in situ hybridization ... necessary for colonization of large intestines of streptomycin-treated mice Infect Immun 67, 6152–6156 [37] Felske, A., 1998 In situ detection of an uncultured predominant Bacillus in dutch grassland ... Boye, M., 1998 Specific detection of the genus Serpulina, S hyodysenteriae and S pilosicoli in porcine intestines by fluorescent rRNA in situ hybridization Mol Cell Probes 12, 323–330 [20] Brown-Howland,...

Ngày tải lên: 30/10/2012, 11:02

... by inversion continually for the first 30 s then two inversions every 30 s Rinse twice with tap water Fix for min, with agitation, with Ilford Rapid Fixer (Subheading 3.5.5.) Rinse in running ... (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries Hum Genet 80, 224–234 Collins, C., Lin Kuo, ... cement from the slides using fine forceps and lift off the coverslips Rinse briefly in the Coplin jar containing 50% deionized formamide/2× SSC at room temperature, from Subheading 3.4.1 RISH to Animal...

Ngày tải lên: 11/04/2014, 09:47

... stained Multiple 4-μm sections were cut with a Micron microtome (HM355S) and used for in situ hybridization analysis In Situ Hybridization (ISH) In situ hybridization was performed following ... now being exploited in cancer, attempting to modulate their expression, reintroducing microRNAs lost in cancer, or inhibiting oncogenic microRNAs by using anti-micro oligonucleotides [38] In a ... automated platform (Prior) Representative and viable tissue sections were scored manually semiquantitatively for cytoplasmic staining on computer screen The dominant staining intensity in tumor cells...

Ngày tải lên: 18/06/2014, 16:20

... system for understanding gene packing in biological systems [26–28] This process prompts a number of analogous studies on coils conformation and inorganic superstructure formation by templating ... to form specific morphology with more complexity in comparison with DNA toroid Thus, a new strategy for constructing a spherical complex (for example, vesicle) using a wide range of polymers for ... however, that has not been studied in previous reports is the loading capability for guest species of the SHNs obtained after washing process In order to determine whether guest molecules can be...

Ngày tải lên: 22/06/2014, 00:20

Ngày tải lên: 28/06/2014, 15:23

... and Yukinori Yoshimura, 52001 A sensitive non – radioactive in situ hybridization method for detection of chicken IgG - chain mRNA: a technique suitable for detecting of variety of mRNA in tissue ... Biotinylated probes for in situ hybridization histochemistry: use for mRNA detection J Histochem Cytochem 41: 1751 – 1754 22 Heitzman H., Richards F.M., 1974 Use of avidin – biotin complex for ... and in situ hybridization in Biomedical Sciences (Eds Beesley J.E.) Boston, Birkhauser, p 108 – 137 19 Schad A., Fahimi H.D., Volkl A., Baumgart E., 1996 Nonradioactive in situ hybridization for...

Ngày tải lên: 28/07/2014, 02:21

... tuyệt đối, xylene chất thay xylene, nƣớc cất hai lần, chloroform, paraffin, formalin, formaldehyde 37%, acid acetic nguyên chất, paraformaldehyde dạng bột, Bismark Brown Y dạng bột, keo dán (Boume ... 12,5 Paraffin đƣợc dùng quy trình xử lý mẫu hỗn hợp với sáp ong theo tỷ lệ paraffin : sáp ong III.5.2.3 Đúc mẫu paraffin Đặt mẫu xử lý vào khuôn inox, dùng bình rót paraffin (5 paraffin : sáp ong) ... dịch Blocking buffer 1X, chất blocking dung dịch khóa phân tử lai không đặc hiệu lại  Ủ mẫu với dung dịch Anti – Digoxigenin (Anti – DIG) có gắn Alkaline phosphatase (AP), Anti – Digoxigenin kết...

Ngày tải lên: 28/07/2014, 02:21

... phƣơng pháp phòng bệnh virus đốm trắng II.5 PHƢƠNG PHÁP IN SITU HYBRIDIZATION II.5.1 Sơ lƣợc phƣơng pháp In situ hybridization Phƣơng pháp In Situ hybridization (ISH) đƣợc phát vào năm 1969 công Gall ... : In Situ hybridization PCR : Polymerase Chain Reaction ELISA : Enzyme Linked Immuno Sorbent Assay RT – PCR : Reverse – transcription polymerase chain reaction PBS : Phosphate Buffered Saline ... 14 II.4.2.5 Phòng trị bệnh 14 II.5 PHƢƠNG PHÁP IN SITU HYBRIDIZATION 14 II.5.1 Sơ lƣợc phƣơng pháp In situ hybridization 14 II.5.2 Khái niệm lai phân tử ...

Ngày tải lên: 28/07/2014, 02:21

... results also showed that dark staining was observed around the nucleus predominately, indicating that β-actin mRNAs existed mainly in the vicinity of the nucleus In order to analyze the correlation ... square is the order of insertion with the AFM probes β-actin mRNAs were detected mainly in the vicinity of the nucleus Usually β-actin mRNA is known to exist mainly in the vicinity of the nucleus, ... (a-c) Each square indicates the region of the AFM probe insertion Numbers in lower panels indicate β-actin mRNA quantities detected by the SCN method, and dark intracellular staining indicates distribution...

Ngày tải lên: 11/08/2014, 00:22

... used for in situ, hybridization has been described elswhere (Gellin et al., 1985), and we added only some modifications concerning the washings after hybridization: the slides were first rinsed in ... by in situ hybridization Cytogenet Cell Genet 39, 220-223 Lefevre F & Labonnardi6re C (1986) Molecular cloning and sequencing of a gene incoding biologically active porcine interferon alpha J Interferon ... explain the efficiency of in situ hybridization This observation confirms the one given by Lin et al 1985 We have also modified the washings after hybridization They are less stringent and, in...

Ngày tải lên: 14/08/2014, 19:23

Ngày tải lên: 19/12/2014, 20:12

... BUILT -IN SENSOR ARRAY FOR IN- SITU MEASUREMENT OF CELLULAR MICROENVIRONMENT ZHANG LIN (B.Eng, ZJU, China) A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF ENGINEERING DEPARTMENT OF BIOENGINEERING ... capability in creating more in- vivo like cellular microenvironment, are breaking new ground for cell culture; yet they also bring new challenges in accurate detecting, sensing and monitoring such in- vivo ... Yuerui (Stanford) for all their kind help during my conference stay in US My deepest acknowledgements go to the National University of Singapore (NUS) for providing financial support for the project,...

Ngày tải lên: 03/10/2015, 11:36

... Fluorescence in- situ hybridization: Fluorescence in- situ hybridization (FISH) is a technology utilizing fluorescently labeled DNA probes (with a fluorescent dye or a hapten, usually in the form of ... can be used in interphase as well as metaphase for analysis of chromosomal anomalies In the interphase it is mainly used for aneuploidy detection In the metaphase it can be used for detection ... anomalies in standard karyotyping for prenatal and pre-implantation genetic diagnosis and cancer cytogenetics 2.5.5: Fluorescence in- situ hybridization (FISH) as a sensitive tool in detecting mosaicism:...

Ngày tải lên: 04/10/2015, 15:45

... cooperatively in the cross-linking of proteins, including involucrin, loricrin and small Preferred substrate peptide for TGase proline-rich proteins (SPRs) Such concerted reactions result in formation ... +1 Interestingly, the amino acid located at this position is not important for the reaction in other Tgases, including TGase In addition, at the N-terminal side of the glutamine residue including ... evaluated for detecting in situ activity of TGase in a frozen mouse skin section As shown in Fig 6, in the presence of CaCl2, specific incorporation of FITC–pepE51 (1 lm) in endogenous glutamine-acceptor...

Ngày tải lên: 29/03/2014, 21:20

... decrease in FRET efciency with stretch, and in convex regions as it actively wiggled (Fig 10G,H) Figure 11 indicates that stFRET integrated into actinin and lamin can sense tension in situ Migrating ... Discussion B Fig a-Helix linker in stFRET is resistant to temperature melting (A) stFRET spectra obtained at 60 C for min, 60 C for min, 70 C for and 80 C for (scan parameters as in Fig 3) (B) stFRET ... stFRET itself distributes, we incorporated it into host proteins, including nonerythrocyte spectrin, lamin A and a-actinin (Fig 10A,C,E) in 3T3 cells, and COL-19 in C elegans (Fig 10G) The distribution...

Ngày tải lên: 30/03/2014, 04:20

... mortality [15] in the emergency department setting This protocol has the more modest goals of reducing costs, avoiding unnecessary testing and inappropriate therapies, and reducing antibiotic ... unnecessary testing and antimicrobial use The protocol will need to be prospectively validated in a controlled fashion in order to confirm these findings as well as to assess its safety Author’s information ... undifferentiated febrile illness in India International Journal of Emergency Medicine 2011 4:57 Competing interests The authors declare that they have no competing interests Received: May 2011 Accepted:...

Ngày tải lên: 21/06/2014, 00:20

... key For example in [6], the authors focus on developing cost-saving mechanisms while weakening the threat model They propose Key Infection, a lightweight security protocol suitable for use in ... are Tiny Public Key (TinyPK) [4] and Tiny Elliptic Curve Cryptosystem (TinyECC) [5] With symmetric cryptography, the simplest idea is to load a secret information in the sensor nodes before their ... D Culler, and J D Tygar, “SPINS: security protocols for sensor networks,” in Proceedings of the 7th Annual International Conference on Mobile Computing and Networking, pp 189–199, ACM Press,...

Ngày tải lên: 21/06/2014, 11:20

... to zero in chains and 4, whereas other chains attain higher throughput than the mean In contrast, this severe unfairness among the saturated chains decreases in Channel MAC by maintaining the ... fair throughput share can be maintained in a single-hop network IEEE 802.11 also maintains fairness which is preserved in a single hop network 5.1.2 Channel Prediction Inaccuracy As we discussed ... MAC still outperforms IEEE 802.11 for all possible values of n in case of imperfect predictions 5.1.3 Linear Chain Scenario We use a 6-node linear chain (i.e., intermediate link/channels) (Figure...

Ngày tải lên: 21/06/2014, 22:20