signal cross talks for sustained mapk activation and cell migration mediated by reactive oxygen species the involvement in tumor progression
Báo cáo khoa học: ERK and cell death: cadmium toxicity, sustained ERK activation and cell death pot
... cascade leading to ERK activation, and in the inactivation of MKP3, which would stabilize ERK activation These two mechanisms could act together to induce the delayed and sustained activation ... least partly responsible for, cell death signaling Reactive oxygen species (ROS) and ERK activation The mechanism responsible for this unusual sustained activation of ERK remains unclear It is well ... role in a vast array of cellular signaling, including the activation of ERK [49] PMA mimics classical cell stimulation by growth factors, and PKC activation results in a strong and transient activation...
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Báo cáo y học: "Endogenous TGF-β activation by reactive oxygen species is key to Foxp3 induction in TCR-stimulated and HIV-1-infected human CD4+CD25- T cells" docx
... antibodies for the indicated time points and then intracellular ROS was stained with DHE The mean fluorescence intensity (MFI) of DHE in a single cell was measured with FACS A aliquot of cells was stained ... antibodies in the presence or absence of MnTBAP (100 μM) for and days The intracellular ROS production was determined by DHE staining Active TGF-β in the supernatants was determined with ELISA The intracellular ... amounts of the purified recombinant TGF-β1 that are provided with the kit Flow cytometric staining The cells were re-suspended in PBS containing 1% BSA and 0.1% sodium azide For the staining of cell...
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PINI and MEK2 regulate BPGAPI induced ERK activation and cell migration
... molecules in signal transduction Firstly, the scaffold protein tethers signaling proteins, which enhances the signaling specificity between signaling proteins and also the efficiency by increasing the ... Ca2+/calmodulin signaling, cytoskeletal architecture, Cdc42 and Rac signaling, MAPK signaling, E-cadherin -mediated cell- cell adhesion and β-catenin -mediated transcription (Briggs and Sacks, 2003) Therefore, ... extracellular domain that binds ligand Upon ligand stimulation, the RTKs dimerize, leading to autophosphorylation of tyrosines in the intracellular kinase domains, thereby creating docking sites for adaptor...
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Signal processing methods for mental fatigue measurement and monitoring using EEG
... is the point between the forehead and the nose; second, the inion which is the lowest point of the skull from the back of the head and is normally indicated by a prominent bump The 10 and 20 in ... weighted SVM and error-correction mechanism is motivated by the special structural information of the learning problem at hand, with the former dealing with the inherent unbalancing of data and the latter ... On the one hand, EEG is subject to many modiers including brain anatomy (for example, the skull insulation distorts the EEG signals), neuron alignment and even metabolism in the brain On the other...
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Báo cáo khoa học: Proteoglycans in health and disease: new concepts for heparanase function in tumor progression and metastasis pptx
... HS cleavage by heparanase is required for structural remodeling of the ECM underlying tumor and endothelial cells, thereby facilitating cell invasion [23–25] Progress in the field and the generation ... inhibit cellular invasion by promoting tight cell cell and cell ECM interactions, and by maintaining the structural integrity and self-assembly of the ECM [10,11] Notably, one of the characteristics ... of the primary tumor [102] The possible involvement of heparanase in tumor selfseeding is supported by the timing of its induction during tumorigenesis and its prometastatic function Using the...
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Báo cáo khoa học: Calcium-induced activation and truncation of promatrix metalloproteinase-9 linked to the core protein of chondroitin sulfate proteoglycans pot
... of the C-terminal domain was lacking These results indicate that the interaction between MMP-9 and the CSPG core protein must involve the most C-terminal part of the MMP-9 hemopexin-like domain ... shortening of the C-terminal domain It appeared that the core protein of the CSPG was cleaved prior to the processing and shortening of the C-terminal hemopexin-like domain, as the 100 kDa form ... urea in the proMMP-9/CSPG preparation had no effect on the position of the two main gelatinase bands (Fig 8, lanes and 2), suggesting that neither the band seen in the stacking gel nor the 300-kDa...
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Báo cáo khoa học: Neuroglobin and cytoglobin expression in mice Evidence for a correlation with reactive oxygen species scavenging doc
... for why the retina is capable of tolerating an almost 10 times longer period of ischemia than brain They described how the presence of hypoxia in the retina is consistent with the finding of increased ... time Therefore, they contain higher levels of HIF-a mRNA, so the HIF-a proteins can be elevated rapidly by de novo protein synthesis using pre-existing mRNA [15] This could possibly explain the ... relative oxygen consumption Although still a matter of debate, the estimated local concentration of Ngb in the mouse retina is > 100 lm, and is thus within the range of Mb in the muscle, indicating the...
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báo cáo hóa học: " Interleukin 1 receptor antagonist knockout mice show enhanced microglial activation and neuronal damage induced by intracerebroventricular infusion of human β-amyloid" pdf
... conceive the study, interpret the results, and assist in the preparation of the manuscript EH developed and provided the IL1raKO mice and gave helpful advice for handling and care of the animals ... IL-1 signaling and other neuroinflammatory mediators and pathways could be utilized to develop clinically meaningful, disease-modifying AD therapies Competing interests The authors declare that they ... the brain may possess more significant pathology following a unilateral infusion and, therefore, confound the results and/ or conclusions, only the right half of the brain was fixed in a paraformaldehyde/...
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báo cáo hóa học: " Prostaglandin E2 receptor subtype 2 (EP2) regulates microglial activation and associated neurotoxicity induced by aggregated α-synuclein" pptx
... hypothesis that the EP2 receptor is critical in regulating aggregated α-synuclein levels in PD, thereby influencing neurodegeneration induced by aggregated α-synuclein via ex-vivo, in vivo and in ... seeded in 6-well plates overnight and then treated with pre-aged α-synuclein or vehicle for 30 The cells were then homogenized and fractionated into cytoplasmic and membrane fractions, and the fractions ... p67-phox and p47phox was analyzed by determining changes in their relative levels in the cytoplasmic and membrane fractions following exposure to aggregated α-synuclein The results, presented in Figure...
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Báo cáo khoa học " Study on macrophage activation and structural characteristics of purified polysaccharide from the liquid culture broth of Cordyceps militaris " doc
... determined by measuring the optical density at 540 nm on a scanning multiwell spectrophotometer The ability of CPSN Fr II to induce production of TNF-␣ in RAW264.7 cells was determined by dissolving ... After incubation for h, the plate was centrifuged at 800 × g for and the supernatants were aspirated The formazan crystals in each well were dissolved in 150 l dimethylsulfoxide and the color generated ... was further incubated for at 37 ◦ C, then for 60 after the addition of U of MuLV reverse transcriptase Reactions were terminated by heating for 10 at 70 ◦ C, and total RNA was depleted by addition...
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Báo cáo y học: "JNK suppression is essential for 17b-Estradiol inhibits prostaglandin E2-Induced uPA and MMP9 expressions and cell migration in human LoVo colon cancer cells" ppsx
... uPA and MMP-9 by suppressing the activation of JNK1/2 in LoVo cells The present study suggests that 17b-estradiol presents the properties of anti-cancer by inhibiting PGE2-induced migration in ... PAI1 and TIMPs in Human LoVo Colon Cancer Cells uPA and tPA is closely controlled by PAI-1 In addition, activation of MMP-2 and MMP-9 was inhibited by TIMPs, therefore we further examined whether ... After incubation for 30 on ice, the supernatant was collected by centrifugation at 12000 g for 15 at 4°C, and the protein concentration was determined by the Bradford method Sample containing equal...
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Báo cáo y học: "Early Inhibition of NF-κB activation and MMP-9 secretion by plasma of human volunteers after ingestion of maritime pine bark extract (Pycnogenol)" pot
... overnight Cells were then stimulated with μg/mL LPS and incubated at 37°C for 60 minutes After incubation the number of viable cells was determined by counting living cells after staining with trypane ... Salmonella minnesota Re 595, Sigma-Aldrich Inc., Taufkirchen, Germany) and incubated at 37°C for 48 hours The number of viable cells was determined by counting living cells after staining with trypane ... penicilline/ streptomycine, % non-essential amino acids and mM L-glutamine) and incubated with monocytes for one hour Cells were then stimulated with 10 ng/ml LPS (Lipopolysaccharides (rough strains)...
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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"
... other group maintained in the medium containing 480 μg/ml Hp extract and μmol/L of DHR-123 for h, h, h, 12 h and 24 h Cells in the blank control were grown in the culture medium alone In the negative ... mtDNA resulting in abnormality in ATP production Therefore, the amount of ATP was further determined aiming to indirectly detect the cell viability and the mitochondrial activity and function ... et al., their results showed Hp infection resulted in increased mutations in the non-coding D-loop as well as the coding genes ND1 and COI of mtDNA of gastric cells [12] The increase in the number...
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Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx
... slow thermal inactivation Examination of the role of peroxide-derived radicals in enzyme inhibition To examine whether radical species were generated during the inactivation of GAPDH and LDH by ... peptide and protein peroxides investigated in the current study, with GAPDH being more sensitive than GR and LDH, and inactivation of GAPDH being metalion independent, whereas inhibition of GR and ... thereby maintains reducing equivalents within the cell Other enzymes, such as LDH, are unaffected, so such damage is selective The long lifetime of these protein peroxides may allow these species...
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Báo cáo khoa học: E2A participates in a fine control of pre-mature B-cell apoptosis mediated by B-cell receptor signaling via transcriptional regulation of survivin, IAP2 and caspase-8 genes pot
... cooperatively induce apoptotic cell death in the DT40 cell line [24] As a first step in elucidating the participation of E2A in apoptosis of DT40 cells, we examined the effects of PMA/ionomycin treatment for ... PCAF and MOZ These findings, together with those shown in Table 1, indicate not only that HDAC2 and GCN5 are necessary for control of the apoptosis of the DT40 cell line mediated by PMA/ionomycin ... the DT40 cell line cooperatively mediated by E2A and BCR signaling, DT40 and E2A)/) were cultured in the presence of PMA/ionomycin, and RT-PCR was performed for various factors (Fig and Fig S2)...
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Báo cáo khoa học: Structure, regulation and evolution of Nox-family NADPH oxidases that produce reactive oxygen species pptx
... disrupted by binding of Ca2+ to the EF-hands [65] In plants, the EF-hands of Rboh localize within the Rac-binding region at the N-terminus, and binding of Ca2+ to the EF-hands prevents Rac from binding ... protein contains two sets of the p67phox core cassette, comprising the Rac-binding TPR domain and activation domain (Fig 17): the sequences of the Racbinding insert region and the activation domain ... shown in blue; and residues of the SH3 domain are shown in magenta A model for the structure of NoxSH3 is also shown The SH3 domain is inserted into the C-terminal NADPH-binding domain Cylinders...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
... were incubated in NaCl/Pi for 90 min, the intracellular fluorescence signal dramatically increased in all cases (Fig 9, Table 1) The increases, in comparison to the signals observed in standard ... mitochondria, the major cellular site for ROS formation, reduces enzymatic and soluble intracellular antioxidant defences but not ROS flux in the studied q0 cells, and that there are cell line-to -cell line ... in q0 than in q+ cells, yet the extent of the increase varied considerably between the three q0 lines In bone and lung q0 cells the increases were 17- and 39-fold, respectively However only in...
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Báo cáo khoa học: Is there more to aging than mitochondrial DNA and reactive oxygen species? pot
... GPx enzymes indirectly protect the cell from damage by the OÀ , by preventing per2 oxide -mediated inactivation of Sod1 [59] Interestingly, Sod itself protects GPx from inactivation by OÀ [60] ... Maklashina and Ackrell [152], critically examined the literature on the role of ETC dysfunction in aging and concluded that the experimental evidence in support of the model of age-related inactivation ... of the respiratory chain can be challenged based on the impurity of the mitochondrial preparations and the inadequacy of assay procedures in the published reports In these author’s opinion, the...
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báo cáo hóa học: " Effects of the cyclooxygenase-2 inhibitor nimesulide on cerebral infarction and neurological deficits induced by permanent middle cerebral artery occlusion in the rat" potx
... played by post-ischemic inflammation The Ca2+-related activation of intracellular second messenger systems, the increase in reactive oxygen species formation, as well as hypoxia itself triggers the ... Systems; Mountain View, CA) Measurements were made by manually outlining the margins of infarcted areas The unstained area of the fixed brain section was defined as infarcted Cortical and subcortical ... stroke induced by the occlusion of the middle cerebral artery using an intraluminal suture, the infarct size progresses very fast in the subcortical areas (mainly striatum) and much slower in cortical...
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Báo cáo y học: " Monocytes are essential for inhibition of synovial T-cell glucocorticoid-mediated apoptosis in rheumatoid arthritis" docx
... evaluated by both CD68 and CD163 staining (data not shown) The decrease in the ST T -cell population is not mediated through apoptosis induction Synovial apoptosis evaluated by TUNEL and staining for ... data and writing of the manuscript PN and EK recruited the patients for the study, performed arthroscopies, and participated in acquisition and interpretation of the data OS participated in the ... factor(s) rather than cellular interaction are essential for the induction of the T -cell apoptosis-resistant phenotype To further investigate the mechanism responsible for the resistance of T cells...
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