secretion in yeast purification and in vitro translocation of chemical amounts of prepro amp 945 factor
Analysis, sequencing and in vitro expression of PCR products
... functional domain mapping of proteins where fragments that represent protein domains are expressed and functionally analyzed using in vitro assays Furthermore, rapid in vitro protein–protein interaction ... separation in thin-coated capillaries containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading and running samples and ... perform cycles of denaturation, annealing and extension on a template using a single primer resulting in linear amplification with simultaneous incorporation of dideoxynucleotide terminators Although...
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Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf
... (DDIs and doxorubicin) The DNA bulge binding of both compounds was detected by CD and UV melting experiments Possible slippage mechanisms are discussed Results and Discussion Effect of DDI-1A and ... several factors including the length of the repetitive unit, its sequence and the characteristics of polymerase In vitro studies on the expansion of triplet repeats such as CAG, CGG and GAA, ... binding pocket, with its amino sugar anchored Expansion of DNA repeat sequences in the major groove of the DNA pointing toward the 3¢-bulge-flanking base pair We were interested in small molecules that...
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In Vitro Screening of Plant Resources for Extra-Nutritional Attributes in Ruminants: Nuclear and Related Methodologies pptx
... emergence and wide-spread incidence of chemical residues in human food and antimicrobial and anthelmintic resistance causing a surge of interest in the use of “natural” alternatives to chemicals in ... the initial screening is conducted in vitro because of the large number of candidate plant species and the prohibitive cost of screening them in vivo The number of species for in vivo testing ... challenges of extrapolating in vitro findings to in vivo evaluation of plant resources The chapters in this book are written by experts interested in exploring and making better use of plant biodiversity...
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Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... ratio of apparent dissociation constants for binding GST-IjBa in the presence and absence of ATP, and the value of a indicates whether the binding of one substrate (ATP) affects the afnity of the ... In vitro analysis of NF-jB signaling pathway 46 Peet GW & Li J (1999) IkappaB kinases alpha and beta show a random sequential kinetic mechanism and are inhibited by staurosporine and quercetin ... 1) A E C Ihekwaba et al containing 100 lgặmL)1 ampicillin A colony of E coli in LB agar plates was inoculated into 50 mL of LB liquid medium and incubated on shaking platform with 200 r.p.m at...
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Báo cáo khoa học: In vitro analysis of the relationship between endonuclease and maturase activities in the bi-functional doc
... available and its binding affinity is not altered by the presence of DNA in the DNA binding site Interestingly, when nM RNA and 10 nM DNA were added simultaneously to a limiting amount of protein in ... stringent concentration of KCl, there was a correlation between inhibition of endonuclease activity and inhibition of splicing activity indicating that RNA binding can be monitored by measuring ... determine the relative stoichiometry of RNA and DNA binding, nitrocellulose filter binding assays were performed (Fig 4A) Both DNA and RNA substrates saturated limiting amounts of protein in the...
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Báo cáo khoa học: Chick embryo anchored alkaline phosphatase and mineralization process in vitro Influence of Ca 2+ and nature of substrates doc
... presence of anchored GPI-bALP (Fig 1) The presence of HA in the mineral samples was confirmed by In uence of other phosphate substrates in the mineralization process To examine the effects of substrates ... quality and the type of mineral formation obtained in the mineralization medium during the GPIbALP-induced hydrolysis of different phosphate substrates Ó FEBS 2003 Bone GPI-bALP, roles in mineralization ... interpretation of IR spectra of mineral deposits Distinct mineralization steps were observed during the GPI-bALPinduced hydrolysis of b-GP At incubation times in the range 1.5–3 h, two bands located...
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in vitro release of ketoprofen from proprietary and extemporaneously manufactured gels
... on the transdermal delivery of various molecules, both in vitro and in vivo These methods include in vitro and in vivo delivery of insulin, mannitol, glucose and heparin (17, 80) Phonophoresis ... events include extrusion of lamellar bodies, loss of nucleus and increasing amount of keratin in the stratum corneum (12) The origins of the cells of the epidermis lie in the basal lamina between ... Effect of molarity and pH on the diffusion profile of ketoprofen .85 Effect of temperature on the diffusion profile of ketoprofen 86 Effect of mass on the diffusion profile of ketoprofen ...
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báo cáo hóa học:" In vitro generation of cytotoxic and regulatory T cells by fusions of human dendritic cells and hepatocellular carcinoma cells" docx
... (obtained with following informed consent and approved by our institutional review board obtained) was isolated and maintained in TIL Media I medium with 10% heat-inactivated FCS, mM L-glutamine, ... cells in vitro assay Methods Cell lines K562 cells (American Type Culture Collection) were maintained in DMEM medium Colorectal carcinoma cell lines (COLP-2 and COLM-6) were maintained in TIL Media ... each vaccination [27,33] Autologous FCs were irradiated, suspended in 0.3 ml normal saline, and underwent up to nine times vaccinations via SC injection in the left inguinal area at 2-week intervals...
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Báo cáo sinh học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strain" pot
... cells (cell lines and PBMC) and MYXV wild type (T1) strain or vaccinal (SG33) strain In bovine cell lines, serial viral passages analysis and infection with both T1 and SG33 expressing LacZ gene ... comparing two different MYXV strains (a wild-type strain (T1) and a cell-cultured attenuated vaccinal strain (SG33) [14]) we verified the stability of the viral tropism in vitro Findings Three bovine ... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood...
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Báo cáo sinh học: "In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pot
... fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); black staining of ... cells resulting in tumor growth inhibition (reviewed in [25] and [1]) In mouse systems, ex vivo transduction of chemokines into tumor cells has provided potent tumor vaccines inducing tumor rejection, ... grown in vitro under three-dimensional conditions using a mixture of collagen and fibroblasts [32,44] Interaction of a2 and b1 integrins on CRC-specific T cells with collagen and the presence of...
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Báo cáo hóa học: " In vitro permissivity of bovine cells for wild-type and vaccinal myxoma virus strains" potx
... cells (cell lines and PBMC) and MYXV wild type (T1) strain or vaccinal (SG33) strain In bovine cell lines, serial viral passages analysis and infection with both T1 and SG33 expressing LacZ gene ... comparing two different MYXV strains (a wild-type strain (T1) and a cell-cultured attenuated vaccinal strain (SG33) [14]) we verified the stability of the viral tropism in vitro Findings Three bovine ... http://www.virologyj.com/content/4/1/94 information concerning interactions between MYXV and bovine cells is available yet In this study, we characterized the infection of bovine cell lines and bovine peripheral blood...
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Báo cáo hóa học: " In vitro effects of selenium deficiency on West Nile virus replication and cytopathogenicity" ppt
... cell lines and/ or due to the method used to induce Se deficiency in our in vitro model In studies employing Jurkat E6 cells, the Se-deficient medium comprised of insulin, transferrin and bovine ... medium containing 1% FBS, insulin and transferrin was adequate for growth of Vero and SK-N-SH cells for 10–12 days Se-deficient cells were maintained for 10 days and passaged every days using serum-free ... achieved in both the Vero and SK-N-SH cell lines In vitro response of Vero and SK-N-SH cells to Se deficiency Figure In vitro response of Vero and SK-N-SH cells to Se deficiency Vero and SK-N-SH...
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báo cáo hóa học:" In vitro migration of cytotoxic T lymphocyte derived from a colon carcinoma patient is dependent on CCL2 and CCR2" pptx
... fixed in buffered formalin and embedded in paraffin a, b: Staining with H&E c, d: Specific brown staining of apoptotic cells by anti-caspase Ab e, f: In situ end-labeling (ISEL); black staining of ... cells resulting in tumor growth inhibition (reviewed in [25] and [1]) In mouse systems, ex vivo transduction of chemokines into tumor cells has provided potent tumor vaccines inducing tumor rejection, ... grown in vitro under three-dimensional conditions using a mixture of collagen and fibroblasts [32,44] Interaction of a2 and b1 integrins on CRC-specific T cells with collagen and the presence of...
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báo cáo hóa học:" In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA" doc
... was incubated at 42°C for 90 min, and terminated by heat inactivation at 70°C for 15 Measurement of GAG levels The level of GAG appearing in the medium of explants, cell cultures, and papain-digested ... metalloproteinases mediating matrix degradation in interleukin 1-stimulated human articular cartilage Matrix 1993, 13:95-102 Vincenti MP, Clark IM, Brinckerhoff CE: Using inhibitors of metalloproteinases ... have definite effects on NO production in the joints These inhibitory effects of HA on cell apoptosis may play a role in its mechanism of action in chondroprotection [38] Our findings indicated...
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báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc
... http://www.ovarianresearch.com/content/2/1/20 The binding of FSH and/ or LH in granulosa cells to its own receptors led to rapidly and nongenomic activation of PKA, p38 MAPK, and PI3K in a cAMP-dependent manner [16] and of ERK1/2 via ... Effect of each specific inhibitor of PKA, p38 MAPK and MEK on meiotic resumption of oocytes and cumulus expansion during in vitro maturation of porcine COCs When COCs were cultured without FSH and ... converting enzyme/a disinteglin and metalloptotease 17; Has2: hyaluronan synhase 2; Tnfaip6; tumor necrosis factor αinduced protein 6; Ptx3: pentraxin 3; PGE2: prostagrandin E2; PTGS2; prostagrandin...
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báo cáo hóa học: " Beyond platinum: synthesis, characterization, and in vitro toxicity of Cu(II)-releasing polymer nanoparticles for potential use as a drug delivery vector" ppt
... fringe bias -2.5 V, entrance plate -31 V, and pole bias V CRI parameters were skimmer gas off, sampler gas off, skimmer flow mL/min, and sampler flow mL/ ICP MS tubing was rinsed in between samples ... synthesis, and metal loading RP conceived of the study, and participated in its design and coordination, carried out in vitro toxicity studies, SEM and EDX analysis and drafted the manuscript Competing ... difference between the amount released at 48 h and that contained in the original loading solution determined Cu loading, resulting in values ranging between 12 and 16 wt.% based on these reactions conditions...
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Báo cáo lâm nghiệp: "Efficient method of micropropagation and in vitro rooting of teak (Tectona grandis L.) focusing on large-scale industrial plantations" pptx
... auxins, it promoted early rooting and increased the final rooting percent- age and the number of roots per explant [21, 22] Putrescine is involved in root induction by increasing the activity of ... the use of putrescine, which in combination with low concentration of auxin, in rooting medium, was essential in stimulating high rooting percentage with high quality of roots, resulting in fast ... reducing vitrification problems and aimed at improving shoot length and rooting percentage, by modifying the medium composition in terms of salt balance, type of gelling agent, by adding pectin of...
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Báo cáo khoa học: "Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System" ppsx
... 12 Lee, E S and Fukui Y Synergistic effect of alanine and glycine on bovine embryos cultured in a chemically defined medium and amino acid uptake by in vitro produced bovine morulae and blastocysts ... Production of viable bovine blastocysts in defined in vitro conditions Biol Reprod 1995, 52, 1410-1417 10 Keskintepe, L and Brackett, B G in vitro developmental competence of in vitro- matured bovine ... and Okuda, K Effect of phosphate, energy substrates, and amino acids on development of in vitro- matured, in vitro fertilized bovine oocytes in a chemically defined, protein-free culture medium...
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Báo cáo khoa học: "In vitro susceptibilities of Leptospira spp. and Borrelia burgdorferi isolates to amoxicillin, tilmicosin, and enrofloxacin" potx
... gniylppus rof noisiviD htlaeH laminA ,CLL erachtlaeH reyaB knaht eW noitadnuoF hcraeseR eniuqE ewoL kcaJ eht dna )TAC( noitadnuoF ygolonhceT dna ecneicS etatS kroY weN eht ,hcraeseR eniuqE rof dnuF ... ,H ihsinoK ,A orihsoK ,K aganoriH ,S eiO 62 2002 ,enyaW ,sdradnatS yrotarobaL lacinilC rof eettimmoC lanoitaN ,08-1 pp 22 loV 2A -13M ,dradnats devorppa ;slaminA morf detalosI airetcaB rof stseT ... CIM eht ,seripsotpel dna eailerrob roF dehsilbatse neeb ton sah setehcorips cinegohtap tsniaga ytilibitpecsus laiborcimitna tset ot dohtem dezidradnats dna mrofinu A aripsotpeL ortiv ni noissucsiD...
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Báo cáo khoa học: " Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos" pps
... gniniatnoc pord muidem noitalupinam a otni derrefsnart dna ,muidem noitalupinam hserf ni eciwt dehsaw ,)24333 tshceoH lm/gµ gniniatnoc ASB-HLT eerf-muiclac( muidem noitalupinam ni nim 51 rof ... ,h rof edimixeholcyc lm /gµ 01 gniniatnoc muidem CVI ni detabucni erew setycoo detalumits-ortcelE 2lCgM Mm 50.0 dna 2lCaC Mm 10.0 gniniatnoc lotinnam Mm 082 ni ces yb detarapes cesµ 06 rof V ... )v/w( %4.0 gniniatnoc muidem 32 -)USCN( ytisrevinU etatS aniloraC htroN saw tnempoleved oyrbme rof muidem )CVI( erutluc ehT stnemirepxe lla ni desu saw hctab emas eht morf diulf ralucillof enicroP...
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