s109a s149a and s178a mutations on core recognition by trim family restriction factors
Báo cáo y học: "Oral keratinocytes support non-replicative infection and transfer of harbored HIV-1 to permissive cells" ppsx
... cycles sequences and PCR conditions were modified from [40] sequences and PCR conditions were modified from [74] up to 120 h post inoculation To determine release of infectious virions, TERT-2 cell ... RG and all authors contributed to the critical appraisal of the data EJ contributed to the early design of the study KR and MH conceived of the study, contributed to the design and coordination ... gene delivery and stable transduction of nondividing cells by a lentiviral vector Science 1996, 272:263-267 Matumoto M: A note on some points of calculation method of LD50 by Reed and Muench Jpn...
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Báo cáo y học: " Dual effect of the SR proteins ASF/SF2, SC35 and 9G8 on HIV-1 RNA splicing and virion production" doc
... proteins on Gag and Env synthesis was further evaluated with respect to virion production and infectivity Influence of SR proteins on virion production and infectivity This was examined by monitoring ... be concluded that overexpression of each one of the SR proteins caused a strong reduction of the unspliced viral RNA in cells, and this had a more pronounced effet on virion production than on ... vector) DNA concentrations were maintained constant by supplementation with the pCLacZ control plasmid which also served to monitor transfection efficiency A: Viral production was monitored by CAp24...
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Báo cáo y học: "Fully-spliced HIV-1 RNAs are reverse transcribed with similar efficiencies as the genomic RNA in virions and cells, but more efficiently in AZT-treated cells" ppt
... representation of templates, primers and products of RTion reactions Schematic representation of templates, primers and products of RTion reactions Only the splice donor sites (SD1 and SD4) and the ... the selection mediated by RTion inhibitors used to treat patients could lead to increased proportion of spliced forms of HIV RNAs and cDNAs, and consequently to unusual encapsidation levels This ... Selective transcription and modulation of resting T cell activity by preintegrated HIV DNA Science 2001, 293:1503-1506 Wu Y: HIV-1 gene expression: lessons from provirus and nonintegrated DNA Retrovirology...
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Báo cáo khoa học: Adaptability and flexibility of HIV-1 protease potx
... carbons) on the unliganded protease structure (shown by yellow carbons) Fig 11 Superposition of SD146 bound HIV-1 protease (shown by green carbons) on the unliganded protease structure (shown by ... 11) Discussion and conclusion Structure alignments are very often used to derive functional information on proteins Distant evolutionary relationships are also detected exclusively by structure ... assume two very different conformations, open and closed, whereas the flaps always assume a closed conformation in ligand-bound structures When ligand-bound and open-flap unliganded HIV-1 protease structures...
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Báo cáo hóa học: " A predominance of R5-like HIV genotypes in vaginal secretions is associated with elevated plasma HIV-1 RNA levels and the absence of anti-retroviral therapy" docx
... also confirmed by review of medical chart data Vaginal secretions were collected from the vaginal vault on Dacron swabs and subsequently eluted in phosphatebuffered solution Supernatant and cell ... anticoagulant tubes, and plasma aliquots were stored frozen at -80°C HIV contained in plasma and vaginal secretion supernatant samples was collected by centrifugation and virion-associated HIV-1 ... design and conducted the statistical analyses RC participated in the design and coordination of the study and facilitated the collection of clinical samples NL processed clinical samples and conducted...
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báo cáo hóa học:" The HIV-1 Non-subtype B Workgroup: An International Collaboration for the Collection and Analysis of HIV-1 Non-subtype B Data" pot
... response among non-B HIV-1 For new drugs and certain combinations, there are insufficient data, even in subtype B HIV-1 Worldwide collaboration, using common data collection instruments and uniform ... RT and protease sequences from persons infected with nonsubtype B HIV-1 Program and abstracts of the 10th Conference on Retroviruses and Opportunistic Infections; February 1014, 2003; Boston, ... International Non-subtype B Workgroup We have established an international workgroup for the collection and analysis of RT and protease sequences and data from persons infected with non-B HIV-1 subtypes...
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Báo cáo y học: " Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages" pdf
... experiments M Li and JR were responsible for vector design and preparation LR assisted in SCID-hu mice generation, CD34 cell reconstitutions into mice, PCR and FACS analysis RA was responsible for ... 85% reconstitution levels with the other being 33% For the two Tar-CCR5Rz construct injected mice, the reconstitution levels were 75% and 30% (Fig 6, panels A and B) Reconstitution levels are known ... pCMV-rev and pCMV-G each as described previously [23] Viral supernatants were collected at 24, 48 and, 72 hrs post transfection, pooled, and concentrated by ultracentrifugation [25] Concentrated...
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Báo cáo y học: "Clade, Country and Region-specific HIV-1 Vaccines: Are they necessary" pptx
... cocktail formulation able to prevent virus infections in every world region, and to overcome the political and financial dilemmas associated with the production of clade, country or region-specific ... superinfection by cloned SIVmac251 in cynomolgus monkeys J Gen Virol 1997, 78:2529-2539 Wrin T, Crawford L, Sawyer L, Weber P, Sheppard HW, Hanson CV: Neutralizing antibody responses to autologous and ... depends on three-dimensional structure, and the molecular structures bound by antibodies can occur on proteins that differ widely in primary sequence T-cells recognize peptides in association with...
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Báo cáo y học: "Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity" docx
... the standardized New Zealand White rabbit vaginal irritation Authors' contributions HS and MAJ carried out differentiation and infection of dendritic cells, macrophages and T cells, isolation of ... citation purposes) AIDS Research and Therapy 2008, 5:10 http://www.aidsrestherapy.com/content/5/1/10 Table 2: Scores of epithelium irritation, leucocytes infiltration, vascular congestion and edeme, ... 0.440 0 0.2 * Mean ± standard deviation £ The scores for epithelium irritation, leucocytes infiltration, vascular congestion and oedeme, were calculated as the mean of the scores estimated at the...
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Báo cáo y học: " Detection of HIV-1 RNA/DNA and CD4 mRNA in feces and urine from chronic HIV-1 infected subjects with and without anti-retroviral therapy" pptx
... hrs and the concentrated supernatant was collected by inverted spinning and further concentrated by ultracentrifugation at 22,000 rpm for one hour at 4°C Most of the supernatant was removed and ... for citation purposes) AIDS Research and Therapy 2009, 6:20 Plus Minipreps DNA Purification System (Promega, Madison, WI) and digested with EcoRI restriction enzyme to confirm the insertion The ... populations in three AC carried out all the feces DNA/RNA isolation and HIV1 detection work and drafted the manuscript LC, MD and CS performed urine DNA/RNA isolation and HIV-1 detection, the...
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báo cáo khoa học: "PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture" ppsx
... experiments and negative control well with agarose only, b) other negative control with tissue alone without treatment and without challenge with virus and c) positive control well with tissue alone ... the control (Fig 2a) The increased proliferation of lymphocytes was presumably due to activation of the immune system, which was induced by the continuous expression of death factors (mutations ... http://www.jnanobiotechnology.com/content/8/1/15 cell-associated infection, even under 'non-optimal' conditions First-generation microbicides are only effective for a few hours and, therefore, require administration shortly...
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Báo cáo y học: " Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages" docx
... Quantification of p24 production in the total T cell fraction and CD4+CD8- T cell fraction derived from hCycT1/CRM1 Tg rats Figure Quantification of p24 production in the total T cell fraction and CD4+CD8- ... hCycT1 and hCRM1 on HIV-1 propagation using a full length HIV-1 clone, we electroporated pNL4-3 into FPM1 T cells that continuously expressed hCycT1 and hCRM1, and then quantified the production ... Characterization of hCycT1 and hCRM1 Tg rats Characterization of hCycT1 and hCRM1 Tg rats (A) The expression of hCycT1 in spleen- and thymus-derived cells from WT or hCycT1 Tg rats was confirmed by Western...
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Báo cáo y học: " Multiple-infection and recombination in HIV-1 within a longitudinal cohort of women" potx
... coinfection (the host was initially infected by two or more strains of HIV-1) and superinfection (an initial infection was followed by a later secondary infection), equal rates of 1.6% for coinfection ... homogeneity on that class alone Intergenic recombination between strains in multipleinfected individuals and selection on recombinants Of the 23 individuals inferred to have multiple infections, only one ... Superscript One-Step RT-PCR for long templates) To avoid contamination among subject visits, all plasma samples from a subject visit were processed for reverse transcription and amplification singly (one...
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Báo cáo y học: "Association of chemokine receptor gene (CCR2CCR5) haplotypes with acquisition and control of HIV-1 infection in Zambians" pdf
... in CCR2 and CCR5 and acquisition or progression of HIV-1 infection Summary of the recent publications on CCR2-CCR5 haplotypes and association with HIV-1 acquisition or disease progression Includes ... Distribution of chemokine receptor CCR2 and CCR5 genotypes and their relative contribution to human immunodeficiency virus type (HIV-1) seroconversion, early HIV-1 RNA concentration in plasma, and ... overall distribution of CCR2-CCR5 haplotypes did not conform to HWE (Table 2) After stratification of the cohort into transmission and nontransmission index partners, seroconverters, and exposed uninfected...
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Báo cáo y học: "Conformational alterations in the CD4 binding cavity of HIV-1 gp120 influencing gp120-CD4 interactions and fusogenicity of HIV-1 envelopes derived from brain and other tissues" pptx
... of controls to ensure equivalent expression of Env on the cell surface, protocols for generating (and measuring CD4 expression on) CD4low and CD4high cells, and the empirical determination of ... CD4bs conformational alterations augment the ability of gp120 to interact with CD4 by increasing the exposure of the CD4bs Repositioning of the V5 loop is associated with conformational alterations ... better understand the molecular mechanisms contributing to alterations in CD4 binding by primary gp120 proteins and the subsequent influence on Env function, we recently developed and validated...
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Báo cáo y học: "Human defensins 5 and 6 enhance HIV-1 infectivity through promoting HIV attachment" ppsx
... Authors’ contributions AR performed the experiments on HIV infection and HIV attachment by HIV p24 ELISA JD performed the experiments on HIV infection and HIV attachment by FACS and microscopy, and ... findings are consistent with our observation that HD6 has a strong tendency to selfassociate in solution and to form high-order aggregates on target molecules (personal communication to W Lu) We ... http://www.retrovirology.com/content/8/1/45 possible role of defensins in enhanced HIV transmission by STIs and BV HD5 and HD6 are constitutively expressed by intestinal Paneth cells and play an important...
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Báo cáo y học: " APOBEC3G-UBA2 fusion as a potential strategy for stable expression of APOBEC3G and inhibition of HIV-1 replication" pot
... ubiquitin directly and inhibits elongation of polyubiquitin chains by capping conjugated ubiquitin [30,39] Since Vif mediates APOBEC3G degradation by promoting protein ubiquitination of APOBEC3G ... degradation, the effects of UBA2 on APOBEC3G protein degradation under the conditions of excessive polyubiquitination or the lack of proteasome activity were examined The effect of UBA2 on APOBEC3G ... protein construct and viral packaging, HEK293 cells that stably express APOBEC3G, APOBEC3G-UBA2, and APOBEC3G-UBA2* fusion proteins were created by proper antibiotic selection High level expression...
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Báo cáo y học: "Selective translational repression of HIV-1 RNA by Sam68DeltaC occurs by altering PABP1 binding to unspliced viral RNA" pps
... being affected by Sam68ΔC Previous analyses had demonstrated that inhibition of HIV expression by Sam68ΔC was lost upon introduction of mutations that disrupted RNA binding capacity consistent with ... export by Crm1 alone is sufficient to confer inhibition by Sam68ΔC or if additional features of the HIV-1 incompletely spliced RNAs (perhaps the Rev/RRE complex) provide additional recognition elements ... each fraction and proteins precipitated by addition of an equal volume of 40% TCA After incubation at C for h, precipitate was collected by centrifugation, washed times with acetone and resuspended...
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Báo cáo y học: "Destabilization of the TAR hairpin leads to extension of the polyA hairpin and inhibition of HIV-1 polyadenylation" pptx
... polyadenylation Results HIV-rtTA expression is reduced by destabilization but not by truncation of the TAR hairpin We previously demonstrated that the TAR-destabilizing A and B mutations induce ... other functions in HIV-1 replication in addition to its role in transcription, such as in translation, dimerization, packaging and reverse transcription of the viral RNA [9-15,4244] (and references ... digested with AatII and BamHI and the 276-bp fragment was isolated and labeled as described above Prehybridization and hybridization was done in ULTRAhyb buffer (Ambion) at 55°C for and 16 h, respectively...
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Báo cáo y học: "Characterization of the HIV-1 RNA associated proteome identifies Matrin 3 as a nuclear cofactor of Rev function" ppsx
... Bio-Rad protein assay kit Additional material Additional file 1: Supplementary information on the characterization of the vectors, on their Rev-responsiveness and on shRNA-mediated knockdown of ... Sanità” of Italy, by the EC STREP consortium 012182 and by the Beneficientia Stiftung and by the Fondo Trieste We recently became aware of a similar work by Kuan-Teh Jeang and collaborators [86] ... organization of replication and transcription Science 1999, 284:1790-1795 17 Chakalova L, Debrand E, Mitchell JA, Osborne CS, Fraser P: Replication and transcription: shaping the landscape of...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25