rflps of variable number of tandem repeats vntrs are the basis for the original dna profiling
Báo cáo sinh học: "A genetic analysis of variable number of tandem repeats (VNTR) polymorphism in the horse potx
... usually consisted of DNA digests from sire and its offspring along with DNA digests from their mothers The sires were also run on the same gel to compare the respective mobilities of their bands (fig ... polymorphism detected with VNTR originates from the number of tandem repeats and not from mutation in the DNA restriction site of the enzyme The banding pattern of the 33.6-HaeIII probe-enzyme combination ... fragments, the sequences of which are more similar to each other and are most probably generated by a greater number of loci The 33.6-HaeIII combination allowed an analysis of the segregation of strongly...
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Báo cáo Y học: IgE reactivity of tandem repeats derived from cockroach allergen, Bla g 1 docx
... replicates, the replication fork ÔslipsÕ from one sequence to another because the end of the nascent DNA shares homology with the repeated sequences on the template DNA A loop of DNA is formed and ... multiple molecular forms of rBla g were investigated PCR analysis of Pichia genomic DNA showed that the origin of the multiple forms of the allergen was not at the DNA level Evidence of recombination ... the N-terminal sequence was used as template (accession no L47595) Inserts were amplified using two primers: one encoding for the start of the N-terminus (MKLAL) and the other for the end of the...
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Informatic analysis of tandem repeats in the human genome
... Structure of gene 21 3.4.1(a) The distribution of number of tandem repeats (period less then 10) through 24 human chromosomes 25 3.4.2(a )The distribution of number of tandem repeats ... represent the repeats period and Z axis stands for number of repeats Fig 3.4.1(a) The distribution of number of tandem repeats (period less then 10) through 24 human chromosomes Fig 3.4.2(a )The distribution ... respectively, and the height of dot denote the copy number of this repeats Figure 4.1.1 illustrates the position and copy number of all the repeats of chromosome 19, while Fig 4.1.2 the repeats in gene...
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Báo cáo y học: " A Novel Variable Number of Tandem Repeat of the Natriuretic Peptide Precursor B gene’s 5’-Flanking Region is Associated with Essential Hypertension among Japanese Females"
... amplification consisted of an initial denaturation at 94°C for min, followed by 35 cycles of 98.5°C for 25 sec, 63°C for 30 s, 72°C for min, and a final extension of 72°C for 10 Then, the PCR products ... initiation codon; Underlining, the variable number of tandem repeat (VNTR) consists of the nucleotides (TTTC) This figure shows the 11 repeat types B: Nucleotide sequence of the 11 and 16 repeat allele ... of the protective factors for hypertension [4] We compared the plasma BNP levels of patients with and without the 16 repeat allele and found that there was no significant difference between the...
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Báo cáo khoa học: "Variable number tandem repeat analysis of Mycobacterium bovis isolates from Gyeonggi-do, Korea" ppt
... parenthesis indicates the number of outbreaks of the genotype of the M bovis isolates Table The allelic diversities determined by the combinations of different VNTR loci Combination of VNTR loci MIRUs ... the allelic diversity (h) with using the following equation: h = − ∑ xi [n/(n−1)], where n is the number of isolates and xi is the frequency of the ith allele at the locus [17] VNTR typing of ... combining the VNTR loci was evaluated and compared (Table 4) When all the VNTRs were combined together, there were 12 different alleles and the allelic diversity (h) was 0.86 The combination of the...
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Báo cáo y học: "Lack of association of a variable number of aspartic acid residues in the asporin gene with osteoarthritis susceptibility: case-control studies in Spanish Caucasians" potx
... detect a D14 effect of the size found in the Japanese [4], with P < 0.05 The power for the comparisons of THR Page of (page number not for citation purposes) Arthritis Research & Therapy Vol No Rodriguez-Lopez ... Spain) JR-L is the recipient of a scholarship of the National Program for the Training of University Professors of the Spanish Ministry of Education AG was supported by the Instituto de Salud Carlos ... similar to the Japanese study (power is 0.16 for the comparison of THR patients versus controls and is 0.29 for the comparison of TKR patients versus controls), probably due to the weak effect of this...
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Báo cáo y học: "Tandem repeats modify the structure of human genes hosted in segmental duplications" docx
... Length of variable ITRs compared to all ITRs in SDs and in the human genome Length of variable ITRs compared to all ITRs in SDs and in the human genome Compared are (a) the total length of the repeats, ... verify the effect of the repeats on the resulting proteins In the majority of cases, the reading frame of the original protein is preserved and variable ITRs cause the elongation of low complexity ... if they had multiple best matches on the genome Groups of paralogs, age of SDs and overlap with human CNVs For each of the 2,008 gene loci, the number of paralogs was defined as the number of...
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Báo cáo sinh học: "Finding the region of pseudo-periodic tandem repeats in biological sequences" potx
... example is from [1] The sequence of the LbH domain of members of the LpxA family consists of the imperfect tandem repetition of hexapeptide units [911] These imperfect tandem repeats (partitions) ... tta The self-alignment of this partition is denotes the length of the string pre(s, i) is the length-i prefix of s and suf(s, i) is the length-i suffix of s Note that the gap at the right end of ... value of a suffix tj of s[1, j] such that there are i letters at the right end of the self-alignment of tj that are aligned with c Note that, the right end of the self-alignment of tj could contain...
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Báo cáo y học: "Mutation patterns of amino acid tandem repeats in the human proteome" ppt
... associated with human disease share a number of characteristics: they are highly polymorphic, they are among the longest tandem amino acid repeats in the proteome, and they are encoded by highly homogeneous ... within the repeat) On the other hand, glutamine, alanine and glutamic acid repeats were the main contributors to the increased number of gap polymorphisms inside the repeats than outside the repeats ... Figure Number 1of polymorphic variants for regions containing different kinds of amino acid repeats Number of polymorphic variants for regions containing different kinds of amino acid repeats For the...
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AMINIMUM NUMBER OF PHYSICAL EDUCATION CREDITS SHOULD BE REQUIRED FOR GRADUATION OF COLLEGE
... one competitor to fail for each competitor that wins Thanks to it, we can learn to share, to be self sacrificed,to work together with others for common goal These are the reasons why I feel physical ... skills, when participating in them, we can recognize the true meaning of cooperation which is defined as a situation in which rewards are distributed equally on the basis of mutual interactive behavior ... the reasons why I feel physical courses should be part of the graduation requirements of any university or college; they are of high value for a student’s health, character-training and his betterment...
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Báo cáo khoa học: Interaction of DAPI with individual strands of trinucleotide repeats Effects on replication in vitro of the AATÆATT triplet docx
... sequences, the specificity of the observed effects confirms the higher selectivity of DAPI for the minor-groove of the ATT-triplet with respect to all the other individual strands of trinucleotide repeats ... repeats, the secondary structures of individual strands are considered to be the main cause of their expansion, and are associated with a number of human genetic diseases [28] In particular, the formation ... attributed to the (ATT)10 region (located at the 5¢-end of the ATT-template) folding into a hairpin-like structure The double helix of the hairpin stem, stabilized by the binding of DAPI into the minor-groove...
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Báo cáo toán học: "Maximising the permanent of (0,1)-matrices and the number of extensions of Latin rectangles" docx
... subpath of the other The root of T (R, v) is the vertex corresponding to the empty path Let ηv,r be the number of closed walks of length r in T (R, v) starting at v and define wr (R) = ηv,2r v The ... consequence of the work of Heilmann and Lieb [8], while the second is due to Joni and Rota [9] Theorem For any R ∈ L(k, n) where k ≥ 2, the roots of ρ G(R), x are real and lie in the open interval ... those which maximise the number of components in G(R) For ≤ n ≤ the maximising × n rectangles are those R for which G(R) ∼ = C10 C6 + C6 C10 + C4 or C6 + 2C4 for n = 5, for n = 6, for n = Here + denotes...
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Báo cáo sinh học: "Detection of cytoplasmic effects on production: the influence of number of years of data" pps
... (with the exception of sex-linked genes in the heterogametic offspring sex) to the genotype of their offspring, it is accepted that progeny performance is affected more by the dam than by the sire ... matrix of order e equal to the number of cytoplasm origins, and I is an identity matrix of order equal to the number of records For model it was assumed that c 0, for model that m and for model ... sets, they were higher for 20 years rather than 10 years Furthermore, table VI, in which the numbers of significant z x values for replicates for each data set are presented, also indicates that the...
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Báo cáo y học: " Analysis of the copy number profiles of several tumor samples from the same patient reveals the successive steps in tumorigenesis" ppt
... joined end-to-end to form the amplitude vector of the whole profile In the following, a refers to the amplitude vector of the whole profile of the tumor The generation of the segment, breakpoint ... the set of tumors removed from the patient At each step of the Page 13 of 19 algorithm, the two closest nodes in the Front, in terms of breakpoints, are joined The copy number profile of their ... random copy number profiles for all the nodes For each condition, 1,000 random trees were generated, and the copy number profiles of the leaves were used as an input for the TuMult algorithm The ability...
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báo cáo khoa học: " Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour" ppt
... differential expression of the many closely related members of the gamma gliadin gene family during grain development Based on the number of ESTs that comprise each of the contigs, the two most highly ... that the sequences of the complement of proteins expressed in a given cultivar are included in the database used for analysis of MS/MS data, so that spectral data will yield a maximum number of ... eliminating the inherent bias that results from amplification of sequences by PCR, the analysis provides new information regarding the expression levels of the genes The data demonstrate that there...
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báo cáo khoa học: " Large-scale polymorphism of heterochromatic repeats in the DNA of Arabidopsis thaliana" ppt
... totals for the A thaliana genome The model is detailed in the Methods section (A) The modeled size of the repeats in the sequenced genome of Col-0 is given for each of these, along with measures of ... in copy number of the repeats; the measured amounts of the centromeric repeat, the pericentromeric transposable element Athila, and 45S rDNA vary by over a factor of two, and the 5S rDNA cluster ... values are presented in regular font, qPCR in bold In the filter arrays, probes for the 18S and 25S subunits of the 45S rDNA gene were pooled; the same value is presented for each subunit The number...
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Báo cáo sinh học: "Comparing the retention mechanisms of tandem duplicates and retrogenes in human and mouse genomes" pdf
... the DDC model only requires that the joint effect of the duplicates fulfil the original functions The signature of positive selection in the retention of retrogenes votes for the prevalence of ... distinct for tandem duplicates and retrogenes, whereas the underlying evolutionary forces are the same As tandem duplicates are generated at the DNA level and easily influenced by gene conversion, they ... according to the divergence time between the two species (Figure 4B) The result shows that, while the duplicates generated prior to the split of the two genomes are more or less the same, there are more...
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Báo cáo y học: "Conservation of tandem stop codons in yeast" potx
... http://genomebiology.com/2005/6/4/R31 Table The influence of fitness effect of the genes on the presence of tandem stop codons Essential genes Weak-effect genes Total With the tandem stop 38 97 135 Without the tandem stop 374 ... ENC) [8] of all the genes with UAA stop codons in S cerevisiae The number of genes with a tandem stop codon located at the UAA+3 location in the high codon bias quartile (25% of genes with the lowest ... stop codon For each codon location in the analysis, the statistical significance (using χ2 tests) of the difference between the frequencies of tandem stop codons in the yeast genes and in the control...
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Báo cáo y học: "High tandem repeat content in the genome of the short-lived annual fish Nothobranchius furzeri: a new vertebrate model for aging research" pptx
... two pairs of the direct descendants of these fish were left One of these pairs was then used for breeding, mainly to preserve the species for the killifish community [11] Offspring of these fish ... repeat structures are formed by the two types of tandem repeats and if these are specific to certain chromosomes, or if and how this impacts on the structural organization of the N furzeri genome ... common type of repeat (37%; Additional data file 5) and cumulatively occupy the third largest amount of sequence compared to the other tandem repeats (Table 3) The repeat motif AC is the most frequent...
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A number of tongue twisters to practice the sounds in English to improve the consonantal pronunciation
... thrilled them • Three Scotch thistles in the thicket • The Catholic author went to the Orthodox cathedral • Is this the sixth, the seventh or the eighth? • The third Thursday of this month is the ... Rumbling in the chimneys, rattling at the doors, Round the roofs and round the roads the rude wind roars, Raging through the darkness, raving through the trees, Racing off again across the great, ... rolled over • As the roaring rocket rose, the restless mosters rollicked • Rustle of trees and ripple of rain, roaring of rivers across the plain • Richard gave Robin a rap in the ribs for roasting...
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