retrospective in vitro studies
Báo cáo khoa học: Bridging the gap between in silico and cell-based analysis of the nuclear factor-jB signaling pathway by in vitro studies of IKK2 ppt
... Screening (Cellomics Inc., Pittsburg, PA, USA); DMEM (Gibco, Invitrogen); 200 mm l-glutamine; fetal bovine serum (Gibco Invitrogen, Virkon, Pzer internal stores) IL-1a (R & D Systems, Minneapolis, ... lL; prewarmed in a water bath at 37 C) was dispensed into each well and incubated for 15 (prewarming xative is critical to maintaining cell integrity) After 15 min, the plates were inverted to ... ndings indicate that substituting previously reported kinetic data in the original model with the experimental data determined here results in an oscillatory pattern analogous to that seen in...
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Báo cáo khoa học: "In vitro studies on the modification of low-dose hyper-radiosensitivity in prostate cancer cells by incubation with genistein and estradiol" pot
... FCS-containing media during genistein incubation and the incubation with higher genistein doses Mechanisms of interaction To search for mechanisms of interaction we investigated protein expression ... Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro: possible involvement of inhibition of survival signal transduction pathways Int ... activity by genistein pretreatment could be shown Furthermore, a significant increase in cleaved PARP protein was measured following combined genistein and radiation treatment, indicating increased apoptosis...
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In vivo and in vitro studies of th17 response to specific immunotherapy in house dust mite induced allergic rhinitis patients
... treatment were used in the following in vivo and in vitro studies 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen ... between in vivo and in vitro findings, confirming that not only Th2, but also Th17 cells play an important role in the context of allergic inflammation IL10 producing CD4+ T cells showed a distinct ... inflammation following SIT therapy In conclusion, our in vivo and in vitro studies presented the evidence that not only Th2 but also Th17 mediated inflammation was involved in the AR pathological...
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In vitro studies on the color stability and masking ability of composite cores and the influence of posts and cores on the shades of all ceramic systems
... extrinsic or intrinsic discoloration46, 85-87 Extrinsic discoloration may result from surface staining, marginal staining due to microleakage and changes in surface morphology by wear The intrinsic ... machining dental porcelain (CEREC) Cerestore injection-molded core Combined etched enamel/porcelain technique to resin bonded restoration Re-introduced the method of etching porcelain, for resin-bonded ... an increase in scattering within the material Light scattering in a material is the result of scattering centers that cause the incident light to be scattered in all directions The scattering...
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Báo cáo khoa học: Insertion of the plant photosystem I subunit G into the thylakoid membrane In vitro and in vivo studies of wild-type and tagged versions of the protein doc
... trypsin, probed with antibodies directed against PSI-G, PSI-K (intrinsic membrane protein, stromal loop), PSI-O (intrinsic membrane protein, luminal loop) and PSI-D (extrinsic membrane protein, ... to leave room for altered protein–protein interactions, which may explain why the Chlamydomonas loop region is exposed to trypsin in the in vitro assay The in vitro import experiments suggested ... disrupting protein–protein interactions within PSI, or by altering the conformation of the loop His-tags were employed in a topological study of the major light harvesting chlorophyll a ⁄ b binding...
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Báo cáo khoa học: Structural studies of the capsular polysaccharide and lipopolysaccharide O-antigen of Aeromonas salmonicida strain 80204-1 produced under in vitro and in vivo growth conditions docx
... and O-chain LPS are composed of linear trisaccharide repeating units containing 3-linked 2-acetamido-2-deoxy-D-quinovose, 4-linked 3-[(N-acetyl-L-alanyl) amido]-3-deoxy-D-quinovose and 3-linked ... determined by the intensity ratio of fragments at m/z 662.2 Da (GalNAcAN-containing trisaccharide repeating unit) and m/z 663.2 Da (GalNAcAcontaining trisaccharide repeating unit) (Fig 4A, inset), ... O-chain containing L-rhamnose, 2-amino-2-deoxy-D-mannose and D-glucose [11], confirming that the novel CPS and O-chain polysaccharide were produced during the 72-h in vivo culture period In addition,...
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báo cáo hóa học:" Functional significance of the signal transduction pathways Akt and Erk in ovarian follicles: in vitro and in vivo studies in cattle and sheep" ppt
... important in mediating the effects of FSH on inhibin-A, activin-A, oestradiol and progesterone secretion and also important in mediating IGF-I stimulated inhibin-A, activin-A, follistatin, oestradiol ... immunization of sheep against an amino terminal peptide of the inhibin αC subunit on intrafollicular levels of activin A, inhibin A and follistatin Journal of Endocrinology 1998, 157:157-168 ... Figure activin-A and follistatinin vitro with control medium, FSH cell number and theper replicate)oestradiol,combination inhibin-Atreating granulosa cells (N = replicates with wells included per...
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Báo cáo hóa học: " Nanostructured Systems Containing Rutin: In Vitro Antioxidant Activity and Photostability Studies" pptx
... solution containing the rutin (120 ml) was added to this organic phase maintaining the agitation for a further 10 Then, the organic phase was injected into an aqueous phase (534 ml) containing Tween ... Biosystems) containing ml of the sample (0.25 mg ml-1) was put into a glass test-tube containing 200 ml of dissolution medium This system was maintained under constant moderate stirring during all the ... rutin-loaded nanocapsules and nanoemulsions, as aqueous intermediate or final systems to the development of nanomedicines containing rutin Both formulations presented an increase in the rutin...
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Probiotic Lactobacillus Strains: in vitro and in vivo Studies pot
... 2000) Experimental design in vivo Eight-week-old inbred BALB/c mice (n = per group) were used for in vivo experiments Strains selected in in vitro tests were mixed in equal proportions, lyophilized ... compared with individual strains (unpublished results); the mixture of bacteria was, therefore, used for in vivo experiment in mice During studies in mouse model the selected strains were safe ... KALLIOMAKI M., KIRJAVAINEN P., EEROLA E., KERO P., SALMINEN S., ISOLAURI E.: Distinct patterns of neonatal gut microflora in infants in whom atopy was and was not developing, J.Allergy Clin.Immunol 107,...
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Báo cáo y học: "Data from necropsy studies and in vitro tissue studies lead to a model for allometric scaling of basal metabolic rate" pot
... References As explained in the PVFCP model, pressure in capillaries necessarily falls as the rate of blood flow in a tissue decreases (assuming that the level of constriction in veins draining the tissue ... log(M) has a slope that is strictly increasing This can be seen by writing the sum of power functions in Equation (3) in order of increasing magnitude of the term scaling exponent as F(y) = α1e y β1 ... scaling estimates from analysis of predicted BMR data is within the experimentally defined confidence interval for the scaling exponent Note that no information on metabolic rates in the studies...
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In vitro and in vivo studies into the antidiabetic and antilipidemic effects of chlorogenic acid
... predominant insulin resistance with relative insulin deficiency or predominant insulin secretory defect with insulin resistance The etiology of this form of diabetes is wide and complicated, ranging ... Activation of insulin signaling upon binding of insulin to insulin receptors causes suppression of gluconeogenesis in liver and kidney [14], translocation of glucose transporter-4 (GLUT 4) from inner ... phosphorylase while stimulating glycogen synthase [17] Increased plasma glucose results in increase in plasma insulin while decrease in plasma glucose causes reduction in plasma insulin level as well II...
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Pathobiological studies of zoonotic blastocystis subtypes using in vitro model systems
... syndrome IFN Interferon IgA Immunoglobulin A IL Interleukin IMDM Iscove’s modified Dulbecco’s medium iNOS Inducible nitric oxide synthase Min Minute ml Milliliter MLC Myosin light chain MLCp Phophorylated ... antigens during chronic infections induces low-grade inflammation in the intestine leading to IBS (Tan et al., 2010) It is also quite possible that an altered luminal environment during IBS is ... Diseases, 15th-16th April 2010, Singapore Haris Mirza, Joshua DW Teo & Kevin SW Tan Role of Rho associated kinase (ROCK) in Blastocystis induced increase in intestinal epithelial permeability The...
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The role of caspase 1 in a murine model of influenza pneumonitis, and studies on cell death inhibitors in vitro
... foetal bovine serum g gram h hours xv H&E haematoxylin and eosin stain HRP horseradish peroxidase ICE interleukin-1β-converting enzyme IFN interferon IL interleukin INF infected IRF interferon-regulatory ... anchored in the envelope, functions as an ion channel between the interior of virus and its environment and maintains a low pH during virion uncoating during initial stages of infection (Pinto, Holsinger ... Protein concentrations in lung airways following an influenza infection 97 3.2.17 Cytokine expression in bronchoalveolar lavage fluid of infection mice as determined by Bio-Plex PROTM Cytokine...
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Biến dị soma trong quá trình nuôi cấy in vitro
... trạng TB nuôi cấy Cây tái sinh Truyền lại cho hệ sau Methionine sulfximine (pseudomonas syringe) Kháng Thuốc + lại độc tốlá bệnh + + Tabtoxin tinh khiết (pseudomonas syringe pv tabaci) Kháng Thuốc ... Kháng amino acid đồng đẳng amino acid Kháng bệnh Kháng thuốc diệt cỏ Chống chịu với stress môi trường Kháng kháng sinh Kháng đồng đẳng base DNA Một số ví dụ thành công chọn lọc invitro đột ... chia làm ba nhóm: sinh lý, di truyền, hoá sinh Hai nguyên nhân gây biến dị dòng soma là: tính không đồng di truyền tế bào soma mẫu cấy ban đầu tác động yếu tố trình nuôi cấy in vitro 3.1 Sự đa dạng...
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Kỹ thuật nhân giống in vitro
... pyridoxine, thiamin, glycine, fonic acid v biotin + Casein hydrolysate, D-Ca- pantothenate, choline chloride, cysteine, malic acid, ascorbic acid, adenine sulfate, riboflavin v glutamine: cú ... dinh dng ph bin: Mui khoỏng: theo White (1943), Heller (1953), Murashige v Skoog (1962) Cht hu c: ng sarcaroza Vitamin: B, B6, inositol, nicotin axit Hoocmon: auxin (IAA, IBA, NAA), Xytokinin ... iu tit sinh trng hoc mụi trng cú cha xytokinin nng thp thỡ phụi phỏt trin thnh cõy hon chnh Bc 5: Thớch ng cõy in vitro ngoi iu kin t nhiờn a cõy t ng nghim ngoi m vi t l sng cao, cõy sinh trng...
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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"
... the loading of pravastatin into erythrocytes was directly proportional with increase of drug concentration in the incubation medium, in the 2-10 mg/ml concentration range This finding is in concurrence ... pravastatin However, the relative impacts of different in vitro findings on the overall in vivo drug delivery efficacy of these cellular carriers remain to be evaluated during the future in vivo studies ... statins including inhibition of the metabolism [5], administration of statins with certain juices [6] or inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase...
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Báo cáo y học: " Preparation of RGD-modified Long Circulating Liposome Loading Matrine, and its in vitro Anti-cancer Effects"
... (GIBCO, USA) containing 10% heat-inactivated fetal bovine serum (Nuoding, China), and the HT-29 cells were grown in DMEM medium (GIBCO, USA) also containing 10% heat-inactivated fetal bovine serum All ... phosphate-buffered saline (PBS), and resuspended at × 106 cells /mL in Annexin-V binding buffer The supernatant (100 μL/tube) was incubated with μL of Annexin-V-FITC (Invitrogen, USA) and μL of PI (Invitrogen, ... ligands In previous studies, PS Reddy et al (14) inserted an RGD motif into the knob domain of an adenovirus so that a re-target vector used integrin as a cellular receptor to dramatically increase...
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Nghiên cứu khả năng tái sinh cây in vitro của một số giống đậu tương
... nuôi cấy tỷ lệ Auxin/ Cytokinin định phân chia tế bào, phân hóa chồi từ mô sẹo, tạo phôi vô tính - Cytokinin gồm có kinetin, BAP, zeatin, 2iP + Kinetin (6 - fafurolaminoprrine) hình thành phát ... chất auxin/ cytokinin môi trường phát sinh quan (rễ chồi) mô sẹo thuốc Khi tỷ lệ auxin/ cytokinin như: IAA/ kinetin < mô có xu hướng hình thành chồi Khi tỷ lệ auxin/ cytokinin > mô sẹo có khuynh ... lượng protein rõ ràng cao hẳn Bên cạnh hàm lượng lớn protein lipit, hạt đậu tương giàu nguồn sinh tố muối khoáng chứa nhiều loại axit amin có axit amin không thay như: Arginin, Histidin, Lysin, Triptophan...
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Nghiên cứu kỹ thuật nhân nhanh chồi In Vitro loài Lan kim tuyến Anoectochilus roxburghii (Wall.) Lindl.
... protocorms with 2-3 cm in length are the most suitable for in vitro rapid multiplication in the most suitable culture medium Knud* that supplemented with 0,5 mg/l BAP + 0,3 mg/l Kinetin + 0,3 mg/l NAA ... results of research on in vitro rapid multiplication of protocorms and shoots of Jewel Orchid species Anoectochilus roxburghii (Wall.) Lindl Knud* medium is the most suitable for in vitro rapid multiplication ... Effect of Environmental Conditions on In Vitro and Ex Vitro Growth of Jewel Orchid Anoectochilus formosanus Hayata, Thesis for the Degree of Doctor of Philosophy in Agriculture, The Graduate School...
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