regulation of gal83 snf4 snf1 kinase activity a glucose exhaustion results in activation and nuclear accumulation of the gal83 snf4 snf1 kinase hetero trimer through an unknown mechanism that is inhibited by glucose gal83 associate
Báo cáo khoa học: The C-terminal region of the proprotein convertase 1⁄ 3 (PC1⁄ 3) exerts a bimodal regulation of the enzyme activity in vitro pdf
Ngày tải lên :
30/03/2014, 08:20
... blotting, amino acid analysis and N-terminal Edman sequencing (data not shown) MS analysis showed that the isolated CT-peptide had a molecular mass within Da of the computed mass 17635.9 Da (average) ... located at the N- and the C-termini can negatively or positively cooperate in the activation of an enzyme In the case of proPC1 ⁄ 3, removal of the various structural and functional domains is a ... or by another enzyme activated by the latter Can the propeptide and the CT-peptide behave synergistically and they share an identical fate? As mentioned previously, there exist instances whereby...
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Báo cáo khoa học: The N-acetylglutamate synthase/N-acetylglutamate kinase metabolon of Saccharomyces cerevisiae allows co-ordinated feedback regulation of the first two steps in arginine biosynthesis potx
Ngày tải lên :
23/03/2014, 21:20
... TTACAAATTAGTTGAAAATTTTGTGAAGTCGTG GTG HP81 ¼ CACTAATTTGTAACCTCTCCTGAT AACCTCTCTTTTTGTGCTGATATTG HP82 ¼ CAA TATCAGCACAAAAAGAGAGGTTATCAGGAGAG GTTACAAATTAGTG AA29 ¼ CGTCAGACCATGG GGTGGAGGAGAATATTCGCGCATGAACTCAAG ... protein, lanes and contain 15 lg, and lanes and contain 30 lg MM is the molecular mass standard expressed and to be stable in the CD truncated kinase, these data indicate that the ASD is insufficient ... BY4 , BY5 : [32], HP72 ¼ GTCTCACAACAACAATTGG CTGTGATCAAGGTG HP73 ¼ CACCTTGATCACA GCTAATTGTTGTTGTGAGAC HP79 ¼ CACACG ACTTCACAAAATTTTCAACTAATTTGTAACCTCT CCTGATCATAG HP80 ¼ CTATGATCAGGAGAGG TTACAAATTAGTTGAAAATTTTGTGAAGTCGTG...
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Báo cáo khoa học: A new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-jB pathway in skeletal muscle pdf
Ngày tải lên :
29/03/2014, 21:20
... CGCCATGGCAATGATGGTACTGAAAGTAGAGG CGGCCCGGGAACTGATTAAGAGTCTGTCG GAGCCATGGAACAACGGAAAAGCGAGAAAC CGGCCCGGGAACTGATTAAGAGTCTGTCG CACCATGATGGTACTGAGAG GAATGTAGCTATGCGAGAGTTC CACCATGGCCGAGTTCAGAAATGGAGAAG ... CACCATGGCCGAGTTCAGAAATGGAGAAG GAATGTAGCTATGCGAGAGTTC CACCATGCTGAAGACACTTCCGGCCAACAG GAATGTAGCTATGCGAGAGTTC CACCATGCTGAAAGCTGCGCTGGAGAAC GAATGTAGCTATGCGAGAGTTC CACCATGACCAAAGTTCCAGTTGTGAAGG GAATGTAGCTATGCGAGAGTTC CACCATGATGGTACTGAGAG ... control, an interaction test of calpain and N 2A- titin is performed (upper left panel) The yeasts carrying calpain and CARP grow on the selective medium, indicating that CARP and calpain interact (upper...
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Báo cáo khoa học: Regulation of the muscle-specific AMP-activated protein kinase a2b2c3 complexes by AMP and implications of the mutations in the c3-subunit for the AMP dependence of the enzyme docx
Ngày tải lên :
30/03/2014, 08:20
... Regulation of AMPK a2 b2c3 complexes by AMP K Lindgren et al all of which are required for its activity [8,9] In the mammalian genome, two isoforms of the a- subunit (a1 and a2 ) and the b-subunit ... domains of the c2 gene, cause Wolff–Parkinson–White syndrome in humans, which is characterized by electrophysiologic abnormalities and hypertrophy of the heart, and also by an increase in cardiac ... presence of any traces of AMP, either endogenous or as a Regulation of AMPK a2 b2c3 complexes by AMP A B C result of the presence of AMP-generating proteins as contaminants [47] Several upstream kinases...
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Accompanying the document Proposal for a REGULATION OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL on European Social Entrepreneurship Funds doc
Ngày tải lên :
30/03/2014, 12:20
... and disabled staff It provides a variety of services: catering and food services, small construction and handicraft jobs and employability training for disadvantaged people In Germany, a business ... a distinct and operational profile and, on this basis, allow for a more rapid development of the sector and the financing tools that are at its disposal 4.7.5 An early approach pre-empts investor ... public finance and charitable support, and challenges in gaining access to so-called 'patient capital' suitably heterogeneous and sustainable sources of private finance that takes a long-term...
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Báo cáo y học: "Regulation of the JNK pathway by TGF-beta activated kinase 1 in rheumatoid arthritis synoviocytes" pps
Ngày tải lên :
09/08/2014, 10:20
... its activation loop [42] It can then engage I-kappaB kinase and MAPK pathways leading to the activation of NF-κB, p38, and JNK [43] TAK1, unlike MEKK1, MEKK2, and MEKK3, is intimately involved in ... blockade of NFkappaB, JNK, and p38 MAPK by a kinase- inactive mutant of the protein kinase TAK1 sensitizes cells to apoptosis and affects a distinct spectrum of tumor necrosis factor [corrected] target ... examined MAP3K gene and protein expression in RA synovial tissue and FLSs [20] These studies showed that four of the MAP3Ks, namely MEKK1, MEKK2, MEKK3 and TAK1, are abundant in RA FLSs Kinase...
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Báo cáo y học: "Early down-regulation of the pro-inflammatory potential of monocytes is correlated to organ dysfunction in patients after severe multiple injury: a cohort study" pptx
Ngày tải lên :
13/08/2014, 16:21
... associations with the clinical course Acta Anaesthesiol Scand 2001, 45:364-370 Gando S, Nakanishi Y, Tedo I: Cytokines and plasminogen activator inhibitor-1 in posttrauma disseminated intravascular ... (interquartile range that contains the 50% of values) with a straight line at the median, a dashed line at the mean, and error bars defining the 5th and the 95th percentiles Differences in the ... Chicago, IL, USA) Results Patient collective and clinical data In this study 13 patients (four females and nine males) were enrolled with an average age of 41 ± years and a mean NISS of 32 ± points...
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A functional genomics approach for elucidation of novel mechanisms involved in GnRH regulation of the gonadotropins
Ngày tải lên :
11/09/2015, 21:47
... two zinc finger motifs Domain D is the hinge region and domain E is the ligand-binding domain (LBD) Adapted from Muramatsu and Inoue, 2000 23 Unliganded ERα is predominantly located in the nucleus ... Genomic organization and functional domains of murine ERα ER has two major transactivation functions (AF), AF-1 (domain B) and AF-2 (domain E and F), which generate surfaces that bind to cofactors ... transcription and noncovalent assembly of the two subunits A B Fig 1.1: Anatomical and functional connections of the hypothalamic-pituitary axis (A) The pituitary is a small, bean-shaped gland...
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Regulation of the TRIP BR1 proto oncoprotein a potential therapeutic target for human cutaneous and intracavitory proliferative lesions
Ngày tải lên :
12/09/2015, 08:19
... especially Prof Bay Boon Huat, Ms Stacy Tan, Ms Geetha Sreedhara Warrier and Ms Malika Raguraman for their kind helps during the last four years in NUS Without the assistance from them and others that ... to include cutaneous and intracavitary superficial mucosal lesions such as vulvar intraepithelial neoplasia, cervical dysplasia or carcinoma in situ, oral cancer and nasopharyngeal bladder cavity ... ovarian carcinoma (Tang et al, 2005) RBT1 mRNA expression is significantly higher in human breast adenocarcinoma cell lines MCF7 and ZR-75, human osteosarcoma cell line SaOS-2 and human non-small...
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Regulation of sla2p by activity in endocytosis and actin organization by the ark1 prk1 family of kinases
Ngày tải lên :
14/09/2015, 08:50
... NPFs in yeast and their domain structures The actin binding domains are highlighted in red (WH2 is Pan1p and Las17p, ADFH in Abp1p and TH2 in Myo3p and Myo5p) and the Arp2/3 binding acidic domain ... Growth and localization of Sla2p C-terminal truncation mutants Effect of Sla2p C-terminal truncation on endocytosis and actin organization Localization and dynamics of Ark1p in Sla2p mutants Interaction ... molecules as well as NPFs These molecules comprise of an Nterminal motor domain, a lipid-binding TH1 domain, an F-actin binding TH2 domain, an SH3 domain and an Arp2/3 binding acidic motif (Moseley and...
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Reactive oxygen species mediated regulation of the na+ h+ exchanger, NHE 1 gene expression a new mechanism for tumor cells resistance to apoptotic cell death
Ngày tải lên :
16/09/2015, 08:31
... with each other in a complex and intricate manner, one group of proteins favoring apoptosis and the other opposing it Thus, the decision to die is a matter of balance amongst anti- and pro-apoptotic ... membrane, carbonic anhydrase activity, and last but not the least transport of acid/base equivalents across cell membrane via specialized transporters In fact many cells face a constant acid load ... NHE1 during ischemia and reperfusion results in increased [Na+] inside the cell that leads to increased intracellular [Ca++] through the Na+/Ca+ exchanger (NCE) and ultimately cell damage and cell...
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Tài liệu Báo cáo khoa học: Regulation of the members of the mammalian heat shock factor family doc
Ngày tải lên :
18/02/2014, 04:20
... more than three repeats and it has been shown that HSF trimers bind to DNA in a cooperative manner, and that the number of trimers bound is reflected in the transactivation capacity [22–24] Although ... steps in the pathway from DNA to RNA to protein, such as control of transcription and mRNA stability, and the relative rate of protein synthesis and degradation Moreover, the mechanisms by which ... the C-terminal activator domain in yeast heat shock factor: independent control of transient and sustained transcriptional activity EMBO J 12, 5007–5018 35 Nakai A, Tanabe M, Kawazoe Y, Inazawa...
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Tài liệu REGULATION OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL doc
Ngày tải lên :
19/02/2014, 14:20
... capital manager These rules also require the manager to have the necessary organisational and administrative arrangements in place to ensure a proper handling of conflicts of interest EN EN Article ... managed by the same venture capital fund manager or the investors in that other qualifying venture capital fund EN Venture capital fund managers shall maintain and operate effective organisational ... capital fund manager is reasonably assured, in light of the nature of the commitment or investment envisaged, that the investor is capable of making his own investment decisions and understanding...
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Tài liệu Báo cáo khoa học: Regulation of the actin–myosin interaction by titin doc
Ngày tải lên :
19/02/2014, 16:20
... thin filaments On the other hand, the facts that the recombinant fragments may have interacted with myosin or the coverslips during the motility assay, and that in some cases they induced actin ... attacked by V8 protease [58] Outside the I-band region, it is likely that the interactions of titin with myosin (in the A- band region) and actin or a- actinin (in Z-disk and its periphery) are strong ... actin and the Troponin I subunit (Fig 4, lanes c and d) Finally, the absence of bands above T800 in all experiments argue against a cross-linking reaction of T800 to actin N-terminal segment Discussion...
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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
Ngày tải lên :
22/02/2014, 07:20
... extracellular region of human and rat CB1 receptor The CB2 antibody was raised against a sequence between the N-terminal and the first transmembrane domain of the protein of the human and rat CB2 ... AEA, PalEtn and 2AG, respectively Analysis of cannabinoid receptors and fatty acid amide hydrolase To determine the presence of the cannabinoid receptors (CB1 and CB2) and of the fatty acid amide ... platelets [34] FAAH, a serine hydrolase and a member of the amidase family, is an integral membrane protein that is responsible for the inactivation of anandamide and, to some extent, 2-AG [35,36]...
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Báo cáo khoa học: Calmodulin-mediated regulation of the epidermal growth factor receptor doc
Ngày tải lên :
06/03/2014, 09:22
... active in the absence of ligands [52] This suggests that membrane integrity could be a prerequisite for maintaining the tyrosine kinase of the EGFR in an auto -inhibited state in the absence of ... operation of this allosteric activation mechanism [85], and also exerts an allosteric control of ligand binding [86] This is a result of the interaction of the distal segment of the JM, particularly the ... segment of the tyrosine kinase domain both electrostatically bind to the negativelycharged inner leaflet of the plasma membrane [54] This maintains the receptor in an auto -inhibited conformation and, ...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
Ngày tải lên :
07/03/2014, 03:20
... N-terminal transit peptide This indicates that: (a) the general Toc/Tic pathway is involved in calcium regulation and (b) a CaM or CaM-like protein is the most likely mediator of this regulation In an ... also display some similar features For example, tissue analysis in Arabidopsis thaliana indicated that both proteins can be detected throughout the plant and that expression does not appear to be ... domain was shown to enhance the ATPase activity of the chaperone Hsp93 The motor activity of this AAA+ ATPase probably accounts for most of the ATP requirement of the import reaction, exerting the...
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REGULATION OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL on the Cohesion Fund and repealing Council Regulation (EC) No 1084/2006 pot
Ngày tải lên :
07/03/2014, 16:20
... quality and interoperable railway systems; (e) enhancing institutional capacity and an efficient public administration by strengthening of institutional capacity and the efficiency of public administrations ... measures Land rehabilitation hectares Total surface area of rehabilitated land Soil sealing hectares Change in land sealed due to development Nature and biodiversity hectares Surface area of habitats ... modification, including the total or partial cancellation, of assistance approved by the Commission on the basis of Regulation (EC) No 1084/2006 or any other legislation applying to that assistance on 31...
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Báo cáo khoa học: Characterization of a cathepsin L-associated protein in Artemia and its relationship to the FAS-I family of cell adhesion proteins pot
Ngày tải lên :
07/03/2014, 16:20
... CLAP_1:AGGACCTTTTATTAGACATTTCAAATATATAATAAACGTTATTTTAAAATTAGAAAAATTGAAAGACAAGCTAATGAAAGCTTATTGCCGATTGGAAAGT CLAP_2:AGGACCTTTTATTAGACATTTCAAATATATAATAAACGTTATTTTAAAATTAGAAAAATTGAAAGACAAGCTAATGAAAGCTTATTGCCGATTGGAAAGT ... CLAP_2:ATTAGTGCTATAGTTTGGGAAATATTTAGTCCTTGTTTTGTGTGATCTTATAAGATAATATTTGTAGTTTGTGCTTTTATATAATTTAGCTCATTGGATT 1730 * 1888 CLAP_1:AAGATCTTCTGAATGTGATTATATGCGGCTGTGTTTTCTAATAGATTTCTAGATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA CLAP_2:AAGATCTTCTGAATGTGATTATATGCGGCTGTGTTTTCTAATAGATTTCTAGATACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA (A) 48 ... CLAP_1:GACATGAAACTGAGAACTCTCCTCACAAAGAGGGACTTGAGGATTAATTTGTATGACAATGGGCAGACAATTCTTGCCGGTGGGAAACGTATAAATGGAT CLAP_2:GACATGAAACTGAGAACTCTCCTCACAAAGAGGGACTTGAGGATTAATTTGTATGACAATGGGCAGACAATTCTTGCCGGTGGGAAACGTATAAATGGAT D...
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Báo cáo Y học: Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii pot
Ngày tải lên :
08/03/2014, 22:20
... between aerobically grown and anaerobically adapted cultures Another cDNA fragment (No 7) indicated similarity to the 5¢ region of the Fe-hydrogenase from bacteria Analysis of the hydA cDNA and genomic ... sequencing A cDNA library was constructed using the Stratagene ZAP Express cDNA synthesis Kit (Stratagene, La Jolla, CA, USA) with lg mRNA of anaerobically adapted cells of C reinhardtii Double-stranded ... targeting domain is probably cleaved by a stromal peptidase at the conserved cleavage motive Val-Ala-Cys-Ala (Fig 2) In addition to the detection of the protein using antibodies raised against the Fe-hydrogenase,...
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