properties of starch from high amylose mutants
The Estimation of Mechanical Properties of Polymers from Molecular Structure ppt
... obtained from Fedors (see Ref 22) with the exception of the value of SO2 where a value of 26,000 was used Internal data of The Dow Chemical Co The data were obtained under conditions of ASTM D-638 ... advantages: Properties can be calculated from only four basic molecular properties These are the molecular weight, van der Waals volume, length of the repeat unit, and the Tgof the polymer Since these properties ... N,N’(p,p’-oxydiphenylene)pyromellitimide] (PI) 1337 ESTIMATION OF MECHANICAL PROPERTIES OF POLYMERS Table I11 Molar Volume Calculated from the Data from Roe and Simha' ~ PS PC PPO POMS PaMS PI CHDMT 373...
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Báo cáo y học: "miRTRAP, a computational method for the systematic identification of miRNAs from high throughput sequencing data" pptx
... products Nearly half of the expanded collection of miR genes (172 out of 380) identified in this study produce moRs from at least one side of the hairpin Indeed, the presence of moRs lends support ... consider pertain to the structure of the hairpin and positions of reads The distance between a miR and moR on the same arm of the hairpin, the offset of the 5' positions of products that overlap at ... proceeds (305 out of 380 in late embryo library) Only 249 out of 380 of the miRs exhibit expression in the adult The low adult expression rate likely results from the unequal contribution of different...
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... affinity with chain 3381 Enzymatic properties of chitinase A from Vibrio carchariae length of chitooligomers implies that the binding site must be composed of an array of subsites, probably six GlcNAc ... isolation of the gene encoding chitinase A and functional expression of the recombinant enzyme in an Escherichia coli system In the present study, the hydro- Enzymatic properties of chitinase A from ... chromatographic peaks of the respective molecules FEBS Journal 272 (2005) 3376–3386 ª 2005 FEBS 3377 Enzymatic properties of chitinase A from Vibrio carchariae Fig Stereochemistry of chitin hydrolysis...
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Báo cáo y học: "Physiological properties of astroglial cell lines derived from mice with high (SAMP8) and low (SAMR1, ICR) levels of endogenous retrovirus" ppsx
... Frequency of sequences in DNA of high, low and non-virus-yielding mouse strains Proc Natl Acad Sci USA 1974, 71:3555-3559 Meeker HC, Carp RI: Titers of murine leukemia virus are higher in brains of ... Establishment of astroglial cell lines from SAMR1, SAMP8 and ICR mice Primary astrocyte cells were cultured from day neonates from SAMR1, SAMP8, and ICR mice [17] Cells were obtained from neonates ... A P value of less than 0.05 was considered significant Results Analysis of expression of the MuLV gene and protein in the brains of ICR, SAMR1 and SAMP8 mice The expression levels of the MuLV...
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Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
... comparison of ecdysteroid receptors A B Fig CLUSTALW amino acid alignment of N-terminal (A ⁄ B) domains of LdEcR and DmEcR (A) Alignment of EcRA from the two species (B) Alignment of DmEcRB1, ... (A ⁄ B) domains of EcR are also divergent in the two insect species [31] (Fig 1), although all of the isoforms from both species share almost complete identity over a stretch of 35–37 amino acids ... Effects of selected agonists on EcR–USP transcriptional activity in the two species In an initial series of experiments, the basal and ligand-induced properties of the three D melanogaster isoforms...
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Tài liệu Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus ppt
... obtained from Novagen Expression of Pt-gdh under the control of araB promoter was induced for h at 30 °C by the addition of 0.2% arabinose when the A600 of the growing culture reached 0.5 The cells from ... torridus of 60 °C, GdhA displayed 88% of its maximal activity The pH optimum of the pure enzyme was determined to be pH 6.5, but at the physiological pH of 4.6 found in the cytoplasm of Picrophilus ... range of ZnCl2 concentrations tested, i.e from 0.05 to mm The influence of Zn2+ on the pH stability of GdhA is most evident after incubation (1 h, 55 °C) at pH 3.5, where, in the presence of the...
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Tài liệu Báo cáo khoa học: The kinetic properties of various R258 mutants of deacetoxycephalosporin C synthase doc
... of penicillin G or ampicillin at a single defined concentration of the 2-oxoacid cosubstrate (2 mM) Results and discussion Purification and characterization of mutants Wild-type DAOCS and all mutants ... The effect of mutations appears to be similar regardless of whether the prime substrate was penicillin G or ampicillin Utilization of 2-oxoglutarate by the R258 mutants The level of 2-OG conversion ... combination of anion-exchange and gel filtration chromatographies The DNA sequences of the R258 mutants and the ESI-MS analyses showed that only Ó FEBS 2003 Co-substrate selectivity of DAOCS (Eur...
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Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx
... poreforming peptide of 45 amino acid residues from the venom of the South African scorpion Parabuthus schlechteri [4] Here we describe the isolation of new pore-forming peptides from the venom of Opistophtalmus ... isolated from scorpion venom A larger scale comparison of sequences of parabutoporin and the opistoporins with cysteine-free peptides isolated from venom of other arthropods showed that the highest ... 50% of growth of the tested organisms at a concentration of lM at most (see Table 3) Opistoporin is the most active, inhibiting 50% of growth of N crassa and F culmorum at a concentration of 0.8...
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Tài liệu Báo cáo Y học: Electrochemical, FT-IR and UV/VIS spectroscopic properties of the caa3 oxidase from T. thermophilus docx
... FT-IR spectroscopic properties of the caa3oxidase from T thermophilus in the presence and absence of cyanide, and compare the observed properties to previous reports on members of the heme copper ... deviations of approximately nm between the signals of horse heart cytochrome c and the heme c in the caa3-oxidase from Fig Oxidized-minus-reduced UV/VIS difference spectra of the caa3oxidase from T ... structure from infrared absorbance spectra and the deconvolution of the amide-I region A particular problem of the assignment in the difference spectra is the superposition of signals from different...
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Tài liệu Báo cáo Y học: Kinetic properties of bifunctional 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase from spinach leaves pdf
... ratio of 6PF2K activity to Fru-2,6-P2ase activity of the smaller form of the bifunctional enzyme was far lower than that of the larger form of the enzyme [11] This is reminiscent of the enzyme from ... concentration of each of these metabolites and of Pi, as discussed previously [1] In conclusion, the kinetic properties of the recombinant enzyme are in agreement with those of the enzyme isolated from ... blots of total RNA from spinach leaves, the same probe hybridized to a single band of % 2500 bp, corresponding to the length of the isolated cDNA (Fig 2A) Expression of the coding region of 6PF2K/Fru-2,6-P2ase...
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Báo cáo khoa học: Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family pdf
... results of a BLAST homology search indicate that the product of this ORF is highly homologous to avoredoxins from other bacteria, especially that of D vulgaris (Hildenborough), with an identity of ... of the logarithms of the concentration ratios for oxidized and reduced forms of the mediator versus that of recombinant avoredoxin Reduction titration of avoredoxin by NAD(P)H The reduction of ... the proteins of known function, the M acetivorans avoredoxin exhibited the lowest rmsd (1.5 A) and the highest Z score (25.5), as expected from the highest sequence identity (30%) of the known...
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Báo cáo khoa học: Engineering and characterization of human manganese superoxide dismutase mutants with high activity and low product inhibition potx
... restoration of MnSOD activity in transformed cancer cells (via transfection of MnSOD cDNA) results in a slowing of tumor growth in mice, as well as alteration of the transformed phenotype of cancer ... presence of an inactive form of the enzyme that can interconvert to an active form, which manifests as an extended region of zero-order decay of superoxide following an initial burst of activity ... and Fig Catalysis of the decay of superoxide by the single mutants of Table all followed Michaelis kinetics The single mutant C140S hMnSOD had a kcat ⁄ Km value identical to that of wild-type hMnSOD,...
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Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
... similar to that of the family 19 chitinase from barley, the best studied of the plant chitinases [9,12,13,27,28] and essentially identical to that of the catalytic domain of ChiC from S griseus ... mg of ChiG per liter of culture) Tests with several substrates showed that removal of the His tag by tobacco etch virus NIa protease (TEV-protease) did not affect the catalytic properties of ... hypothesis is not confirmed by the present analysis of enzymatic properties of ChiG, or by our analysis of the ChiG and ChiC structures Despite the deletion of the B-loop, the two putative catalytic residues...
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Báo cáo khóa học: Localization, purification and properties of a tetrathionate hydrolase from Acidithiobacillus caldus pot
... hydrolase of A caldus (Eur J Biochem 271) 279 The biochemical properties of tetrathionate hydrolase of A caldus were similar to those of other acidithiobacilli (Table 4) The specific activity of tetrathionate ... production of p-nitrophenol was measured spectrophotometrically at 405 nm One unit of the acid phosphatase activity was defined as the amount of protein which catalysed the formation of lmol of p-nitrophenol ... SD) from two independent experiments Table Purification of tetrathionate hydrolase from A caldus One unit (U) is defined as the amount of tetrathionate hydrolase required for the conversion of lmol...
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Báo cáo khoa học: A new clan of CBM families based on bioinformatics of starch-binding domains from families CBM20 and CBM21 potx
... features of a real SBD [66] although it appears away from the other CBM20 modules of actinomycetes [25] With regard to the large cluster of SBDs from Bacillus CGTases, the positions of the modules from ... glucoamylases (four of them were from patents), one GH77 4-a-glucanotransferase, one genethonin-1 (from rat), five unknown proteins of animal origin (four from insect and one from fish), two carbohydrate ... ribbon diagram of CBM20 module The X-ray structure of SBD from Bacillus circulans strain 251 CGTase (PDB code: 1CDG [33]) The side chains of the aromatic residues involved in the starch- binding...
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Báo cáo khoa học: Purification and properties of a new S-adenosyl-Lmethionine:flavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot
... plot of 1/v vs 1/[s] Enzyme concentration was lM while the substrate was used at concentrations ranging from 0.15 to lM Fig PAGE of the purified flavonoid 4¢-OMT from carnation The position of molecular ... base of the amount of its kaempferide methylated derivative (tR 4.68 min) formed in the course of time Fig 10 Lineweaver–Burk plot of F 4¢-OMT activity for kaempferol at different concentrations of ... Shatz, G & Grisebach, H (1982) Enzymic synthesis ¨ of lignin precursors Purification and properties of 4-coumarate: CoA ligase from cambial sap of spruce (Picea abies L.) Eur J Biochem 123, 583–586...
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Báo cáo Y học: Spectroscopic characterization and ligand-binding properties of chlorite dismutase from the chlorate respiring bacterial strain GR-1 ppt
... E/D ratios of the high- spin signals were calculated from the effective g values by numerical diagonalization of the energy matrix for S ¼ 5/2 [7] Determination of the midpoint potential of the iron ... enzyme from GR-1 [4] However, the existence of a second high- spin species, which we have designated the ÔbroadÕ signal, has not been reported previously The nature of the multiplicity of these high- spin ... evidence from the spectroscopic and ligand-binding studies of chlorite dismutase suggest binding of chlorite to the five-co-ordinate high- spin ferric form of the enzyme as the first step of the catalytic...
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Báo cáo Y học: Properties of group I allergens from grass pollen and their relation to cathepsin B, a member of the C1 family of cysteine proteinases pdf
... fragmentation of the b-expansin at the end of the growth phase was also shown, possibly protecting the growing wall from rupturing Concurrent with this, exogenous application of large amounts of expansin ... another report [38] caused bursting of root hairs, underlining the importance of effective down-regulation of expansin activity Mutagenesis of His104 in the highly conserved HFD motif stabilizes ... wall extension [17] A similarity of the C-terminal region of expansins to CBDs of bacterial cellulases was further suggested on the basis of conserved spacing of Trp residues [40] As shown in...
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Báo cáo khóa học: Functional properties of the protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus A member of a novel protein family related to protein disulfide-isomerase doc
... mixture of 10 mL taken from each of the five reaction mixtures The method consists of adding various iodoacetamide and iodoacetate ratios to portions of the protein to generate a complete spectrum of ... Construction of E coli Pf PDO and mutants Pf PDO(C35S), Pf PDO(C146S) and the double mutant (C35S/C146S) Isolation of chromosomal DNA from P furiosus was performed as described by Barker [15] From the ... investigate the role of the putative redox sites of PfPDO, three mutants were constructed (C35S, C146S, and C35S/C146S) by mutagenizing the most exposed cysteines of each of the redox sites: specifically,...
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