promoter dependent transcription by s pombe rna polymerase iii of preassembled immobilized trna transcription com
Báo cáo y học: "Atypical clinical presentation of a subset of patients with anti-RNA polymerase III - nonscleroderma cases associated with dominant RNA polymerase I reactivity and nucleolar staining" potx
... dilutions C) Anti-RNAP III levels by ELISA Sera from 21 cases with anti-RNAP I /III were tested by ELISA Six cases of atypical presentation (Table 1) and 15 cases of typical presentation of SSc are shown ... phenomenon; SLE: systemic lupus erythematosus; SS: Sjögren s syndrome; SSc: systemic sclerosis: scleroderma; Topo I: topoisomerase I; UFCAD: University of Florida Center for Autoimmune Diseases Acknowledgements ... similar to systemic sclerosis sine scleroderma, in particular with detection of antiRNAP III Similar cases of scleroderma renal crisis with minimal or no features of SSc, some with antiRNAP III...
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Báo cáo khoa học: De novo RNA synthesis by a recombinant classical swine fever virus RNA-dependent RNA polymerase pot
... of RNA synthesis [36] Previous work has shown that crude extracts of recombinant CSFV NS5B expressed in insect cells could produce two RNA products using D -RNA (an mRNA of the liver-specific transcription ... RNA synthesis using (–)IRES as template To optimize conditions for de novo RNA synthesis by the NS5BD24 protein, the 3¢-end of minus-strand RNA (–)IRES, was used as template and newly synthesized ... 3¢-UTRfor2, complementary to the synthesized plus- and minus-strand RNAs, were used for RT-catalyzed cDNA synthesis, followed by PCR amplification of cDNAs Six microlitres of dissolved RNA was subjected...
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Báo cáo sinh học: " Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps" pptx
... respiratory syncytial virus (RSV) [8] by reverse genetics These systems are based on cytoplasmic transcription of viral cDNA by coexpression of phage T7 RNA polymerase Recovery of infectious viruses was ... Cloning of RSV-F expression plasmids and RSV-F expression studies were performed by NT DS analysed expression of VSV-G and synthesised required plasmids SK supervised and participated in VSV-G experimental ... demonstrate striking differences in the requirements for expression of genes of cytoplasmic RNA viruses by DNA expression plasmids The use of codon optimised expression plasmids allowed exclusion...
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Báo cáo hóa học: " Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps" ppt
... respiratory syncytial virus (RSV) [8] by reverse genetics These systems are based on cytoplasmic transcription of viral cDNA by coexpression of phage T7 RNA polymerase Recovery of infectious viruses was ... Cloning of RSV-F expression plasmids and RSV-F expression studies were performed by NT DS analysed expression of VSV-G and synthesised required plasmids SK supervised and participated in VSV-G experimental ... demonstrate striking differences in the requirements for expression of genes of cytoplasmic RNA viruses by DNA expression plasmids The use of codon optimised expression plasmids allowed exclusion...
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Báo cáo sinh học: " A bridge to transcription by RNA polymerase" pot
... omitted) Recombinant RNAPs may be expressed, purified, assembled and characterized in batches of 96 RNAP variants over 1-2 days, making large-scale analysis of systematically designed RNAP substitution ... complexes designed for crystallographic studies are unavoidably compromised by alterations made to prevent phosphodiester bond formation In some structural studies of eukaryotic RNAPs, as with those ... complexes in critical aspects A second limitation of crystal structures is that they only provide snapshots of stable conformations, failing to fully reveal the dynamics involved in catalysis...
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Báo cáo khóa học: Three cyclin-dependent kinases preferentially phosphorylate different parts of the C-terminal domain of the large subunit of RNA polymerase II potx
... thus stressing the specificity of CDK7/CycH/MAT1 and CDK8/CycC for S5 of the YSPTSPS consensus and the preference of CDK9/CycT1 for S2 None of the substrates was phosphorylated in the absence of ... can suppress the activities of these kinases Materials and methods Expression vectors The baculoviruses for the expression of CDK7, MAT1, CycC and His6-CDK9/CycT1 were as described previously ... decrease of the phosphorylation of GST-CTD(27–52) We tested this possibility by performing kinase assays with a GST-CTD(42–52) substrate In Fig 5, we show that all three kinases poorly phosphorylated...
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Báo cáo sinh học: " Open Access Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" docx
... ribosomal RNA (rRNA) genes are transcribed by RNA pol I into a large 4 5S pre-rRNA which is subsequently processed into mature 1 8S, 5. 8S and 2 8S rRNAs Although the mature rRNA sequences are conserved ... Comparison of sequences flanking RNA pol I promoter tranComparison of sequences flanking RNA pol I promoter transcription initiation sites Sequences adjacent to known RNA pol I promoter transcription ... BbsI sites, with the pol I promoter driving expression of influenza vRNA sequences and the CMV pol II promoter directing synthesis of viral mRNA Generation of FluMist® strains from eight plasmids...
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Báo cáo hóa học: " Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells" pdf
... ribosomal RNA (rRNA) genes are transcribed by RNA pol I into a large 4 5S pre-rRNA which is subsequently processed into mature 1 8S, 5. 8S and 2 8S rRNAs Although the mature rRNA sequences are conserved ... Comparison of sequences flanking RNA pol I promoter tranComparison of sequences flanking RNA pol I promoter transcription initiation sites Sequences adjacent to known RNA pol I promoter transcription ... BbsI sites, with the pol I promoter driving expression of influenza vRNA sequences and the CMV pol II promoter directing synthesis of viral mRNA Generation of FluMist® strains from eight plasmids...
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Báo cáo hóa học: " The use of RNA-dependent RNA polymerase for the taxonomic assignment of Picorna-like viruses (order Picornavirales) infecting Apis mellifera L. populations" pptx
... of CBPV (which remains unassigned), the honeybee viruses analysed in this study have been assigned or tentatively assigned (these will be discussed as assigned viruses for the purpose of this ... of using the RdRp as a marker for studying viruses infecting honeybees Results Analysis of RdRp conserved domains across the order Picornavirales The recently defined order Picornavirales has ... comparison of the consensus amino acid sequence for each group supports the current classification of these viruses together within this order and suggests that their RdRp share similar properties...
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Báo cáo y học: " Ubiquitous RNA-dependent RNA polymerase and gene silencing" pptx
... the levels of siRNAs for these trans osons p was also found RNAi knockdown of Elp1 specifically reduced the levels of antisense RNAs for the affected transposons, whereas depletion of Dicer-2 ... in somatic tissues One proposed role of these small RNAs is as a defense against transposable elements The terminal structure of piRNAs is not like that produced by Dicer [19] and so they are ... one aspect of its broader role in gene expression Nevertheless, the antiviral functions of small RNAs act post-transcriptionally and the relationship of these to transcriptional silencing is still...
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Báo cáo y học: "RNA polymerase mapping during stress responses reveals widespread nonproductive transcription in yeast" docx
... example), consistent with the discovery that most of the expression changes in response to a given stressor correspond to a shared environmental stress response [18] Dramatic gains or losses of PolII ... non-TATA promoters Alternatively, we favor a model based on trailing polymerases; stress genes in yeast exhibit ‘bursts’ of polymerases rather than the more evenly spaced polymerases seen at growth/housekeeping ... in stress (see, for example, altered SUT expression in stress in [36]) As genome-wide datasets that identified CUTs and SUTs in budding yeast were not derived under stress conditions, these putative...
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Báo cáo y học: "Tetra-O-methyl nordihydroguaiaretic acid (Terameprocol) inhibits the NF-B-dependent transcription of TNF-a and MCP-1/CCL2 genes by preventing RelA from binding its cognate sites on DNA" ppsx
... concentration was 0.1% in all assays This concentration of DMSO was tested in all assays and did not affect the results LPS from Salmonella Minnesota R595 was purchased from LIST Biological Laboratories, ... and antisense (5’-GAGGCTCAGCCCCCTGTT-3’) Statistical Analysis All graphs and statistical analyses were produced using Prism software (GraphPad Software Inc., La Jolla CA) Results TMP acts early ... RANTES/CCL5 was included since it s promoter does contain NF-B binding sites, although previous studies showed that its expression was not blocked by TMP As shown in Oyegunwa et al Journal of Inflammation...
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Báo cáo khoa học: " Avian reovirus L2 genome segment sequences and predicted structure/function of the encoded RNA-dependent RNA polymerase protein" docx
... putative RNA polymerase to polymerases of positive-strand RNA viruses Nucleic Acids Res 1989, 17:5394 Bruenn JA: Relationships Among the Positive Strand and Double-Strand Rna Viruses As Viewed ... Through Their RnaDependent Rna- Polymerases Nucl Acids Res 1991, 19:217-226 Bruenn JA: A structural and primary sequence comparison of the viral RNA -dependent RNA polymerases Nucl Acids Res 2003, 31:1821-1829 ... identity values > 75%, suggesting these polymerase regions (ARV residues G516LRNQV QRRPRTIMP530, H542TLS/CADYINYHMNLSTTSGSAV563, and T677TTFPSGSTATSTEHTANNSTM698, that correspond to MRV residues G516LRNQVQRRPRSIMP530,...
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Báo cáo y học: "Cell cycle G2/M arrest through an S phase-dependent mechanism by HIV-1 viral protein R." pps
... generally leads to S phase arrest, but not G2 arrest In another study, by using siRNA, a special isoform of PP2A was shown to play an essential role in the G2 arrest induced by Vpr in human cells Unlike ... adenoviral transduction by flow cytometric analysis (A) Expression of endogenous or siRNA-resistant Chk1 constructs from indicated cell lines was confirmed by Western blot analysis using anti-Chk1 ... mutagenesis of the siRNA-resistant Chk1 -S3 45 phosphorylation-site was carried out by the using same procedure as described above with specific nucleotide mutant built in the primers In this case,...
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Nhp6p and med3p regulate gene expression by controlling the local subunit composition of RNA polymerase II
... ribosomal RNA (rRNA), which is processed into 2 8S, 5. 8S and 1 8S rRNA RNA polymerase II catalyzes the transcription of DNA to synthesize the precursors of mRNA and four of the five small nuclear RNAs ... RNAs that take part in RNA splicing RNA polymerase III is needed for the synthesis of transfer RNA (tRNA) and other small nuclear RNAs (including the small 5S rRNA) (Lewin, 2004) RNA polymerase ... polymerase II Transcription in eukaryotic cells is divided into three classes Each class is transcribed by a different RNA polymerase RNA polymerase I, functions in the transcription of precursor...
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MECHANISTIC CHARACTERISATION OF DENGUE VIRUS RNA DEPENDENT RNA POLYMERASE NON NUCLEOSIDE INHIBITOR BINDING POCKET THROUGH IN VITRO BIOCHEMICAL ASSAYS AND REVERSE GENETICS ANALYSES
... coordinators of this programme most especially Prof Marcel Tanner of Swiss TPH and Prof Markus Wenk of National University of Singapore (NUS) and also to our two ladies; Ms Christine Mensch and Ms Susie ... 1.10.4 NS3 It is the second largest protein with a size of ~70kD NS3 is known to possess several enzymatic properties basically required in RNA synthesis and polyprotein processing NS3 is also found ... dengue viruses has led to the association between specific genotypes (within serotypes) and the presentation of more or less severe disease It has been suggested that the immune status and possibly...
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Discovery of safe anti dengue virus drugs from libraries of FDA approved drugs and plants through screening against viral RNA dependent RNA polymerase activity
... polyprotein This is then co- and posttranslationally processed by a combination of both viral and host proteases (Figure 1.4) The NS2B3 serine protease is responsible for NS2A/NS2B, NS2B/NS3, NS3/NS4A and ... synthesis systems In this study, DENV-2 NS5 protein synthesized using the wheat germ cell-free protein synthesis system will be presented as an alternative to other present protein synthesis methods ... (Halstead, 1970) Halstead and his colleagues were one of the first proponents of this hypothesis after early studies in the 195 0s that suggested that DHF/DSS occurs 15–80 times more commonly in secondary...
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the book of css3 - a developer's guide to the future of web design - by peter gasston
... BOOK OF CSS3 THE BOOK OF CSS3 A Developer 's Guide to the Future of Web Design by Peter Gasston San Francisco THE BOOK OF CSS3 Copyright © 2011 by Peter Gasston All rights reserved No part of this ... understand how and when you can use CSS3 and why it has such varied implementation across different browsers A Brief History of CSS3 The version of CSS in current use is CSS2.1, a revision of the ... included in this book across the different versions of browsers, and the second is a list of online resources, useful tools, and interesting demonstrations A website accompanies this book at http://www.thebookofcss3 .com/ ;...
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Tài liệu Teacher''''s Guide: THE IMMORTAL LIFE OF HENRIETTA LACKS by Rebecca Skloot pdf
... implications for local communities resulting from the presence of these industries One of the important issues raised by Skloot s book is the ethics of journalism What constitutes ethical journalism? Compare ... to test his hypothesis about HeLa Who were the test subjects in Southam s first study? Were they informed about the research and its risks? What was the result of Southam s first research study? ... Based on these results, did his hypothesis appear to be correct? Where did Southam find test subjects for his second research study? Based on the results of the second study, what two things...
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