... that probably resultedin a compact conformation that was resistant to cleavage.CD analysis and oligomerizationThe far-UV CD spectrum of ASP3c at neutral pH (Fig. 5A) displayed apositive peak ... that brominated fatty acid and ASA bothassociated with W81 in the same ligand binding site.The ligand binding activity of ASP3c was furtherinvestigated using displacement of ASA, a fatty acid ... purification of recombinant ASP3c. (A) SDS/PAGE analysis of recombinant ASP3c secreted by Pichiapastoris. Lane 1 shows standards (Low range and Polypeptide kits,Bio-Rad, France) and lanes 2–5 are...
... separated by centrifugation at 25 000 g for 20 min into a supernatant and an organellar pellet fraction. Equal amounts of each fraction were loaded onto an SDS gel and subjected towestern blot analysis. ... conditionsFor all plasmid amplifications and isolations Escherichiacoli strain DH 5a was used (Invitrogen, Carlsbad, CA,USA). The yeast wild-type strain BY4742 was used. Thestrain BY4742pex5D was obtained ... Media for the culti-vation of yeast and bacterial strains were preparedas described elsewhere [23,24]. N. crassa strain FGSC#987(St. Lawrence 74-OR23- 1A, mat A) was obtained from theFungal...
... ataxia telangiectasia mutated (ATM) ⁄ataxia telangiectasia and RAD53 related (ATR)kinases at the site of DNA damage may decrease thekinase ⁄ phosphatase ratio and allow the phosphatase todephosphorylate ... withcH2AX as opposed to a transient interaction with Rad53p.AbbreviationsATM, ataxia telangiectasia mutated; ATR, ataxia telangiectasia and RAD53 related; 4NQO, 4-nitroquinoline 1-oxide; FACS, ... to DNA damage byactivating a signal transduction pathway that leads tophosphorylation of Rad9p by Mec1p. Rad9p phos-phorylation allows recruitment of Rad53p to Mec1–Rad9, facilitating Rad53p...
... pro-tein that associates with nuclear matrix. DNA Cell Biol17, 849–858.24 Lee JY, Nakane Y, Koshikawa N, Nakayama K,Hayashi M & Takenaga K (2000) Characterization of a zinc finger protein ZAN75: ... Thanumalayan S, Muralikrishna B,Rangaraj N, Karande AA & Parnaik VK (1999) Colo-calization of intranuclear lamin foci with RNA splicingfactors. J Cell Sci 112 , 4651–4661.11 Gueth-Hallonet ... respectively. A3 con-tains 1 lgofHis6-ISP36 purified by Ni-agarose beads. A1 A3 are stained with Coomassie Brilliant Blue. A4 A6 are the same as A1 A3 except that the samples were blotted onto a nitrocellulose...
... severalauthors, see Asakawa 1, Agarwal and O’Regan 2, O’Regan 3, Habets and Zanolin 4,Xu and Ma 5,Yang6, and the references therein. The main tools in 1–6 are the method of lower and ... Principles in Mathematical Physics, Geometry,and Economics: Qualitative Analysis of Nonlinear Equations and Unilateral Problems, Encyclopedia of Mathematics and Its Applications, no. 136, Cambridge ... 1994.5 X. Xu and J. Ma, A note on singular nonlinear boundary value problems,” Journal of MathematicalAnalysis and Applications, vol. 293, no. 1, pp. 108–124, 2004.6 X. Yang, Positive solutions...
... pagesdoi:10.1155/2010/878131Research ArticleExistence and Multiplicity of Positive Solutions ofa Boundary-Value Problem forSixth-Order ODE with Three ParametersLiyuan Zhang and Yukun AnNanjing University of Aeronautics ... solutions of fourth-order boundary value problems with two parameters,” Journal of Mathematical Analysis and Applications, vol. 281, no. 2, pp. 477–484, 2003.8 S. Fan, “The new root formula and ... has a triple root if A B 0,2 Equation 2.1 has a real root and two mutually conjugate imaginary roots if ΔB2−4AC > 0, 4 Boundary Value Problems3 Equation 2.1 has three real...
... S. G. Hristova and D. D. Ba˘ınov, “Existence of periodic solutions of nonlinear systems of differentialequations with impulse effect,” Journal of Mathematical Analysis and Applications, vol. ... theorems are well known cone theoretic fixed point theorems. SeeLakshmikantham 8 for proofs and details.Theorem 2.6. Let X be a Banach space and K a cone in X. Assume that Ω1and Ω2are bounded ... toLemma 3.5 and the proof is done.Lemma 3.7. Assume D1 ∼ D6. Then every nonempty chain in A has a unique supremum in A. Proof. Let C be a chain in A. Without loss of generality, we may...
... in a mutant thatlacks NirF; this too is not trivial as the DnirF straindoes not accumulate readily detectable amounts of anintermediate of d1synthesis.Experimental proceduresDNA manipulationsDNA ... understood. Anal-ysis of insertional mutagenesis and complementationwork in Pseudomonas aeruginosa, Pseudomonas fluores-cens, Paracoccus denitrificans and Pseudomonas stutzerihave shown that a set of ... 23 Chang CK (1994) Heme d1and other heme cofactorsfrom bacteria. Ciba Found Symp 180, 228–238.24 Van Spanning RJ, Wancell CW, De Boer T, HazelaarMJ, Anazawa H, Harms N, Oltmann LF &...
... CREB-bindingdomain of CBP [31]. The KIX domain of CBP is a structural platform that is capable of binding severaldifferent families of transcriptional activators [30], andevidence indicates that the ... coordinate function of the H3 K4 methyltransferase MLL1 and the H4 K16acetyltransferase MOF. Cell 121, 873–885.17 Yokoyama A, Wang Z, Wysocka J, Sanyal M, AufieroDJ, Kitabayashi I, Herr W & ... The Authors Journal compilation ê 2010 FEBS 1841 of cell fate. Leukemias associated with loss -of- functionor gain -of- function variants of MLL1 are prime exam-ples of the importance of maintaining...