polymerase chain reaction rt pcr
Báo cáo y học: "Invasive and noninvasive methods for studying pulmonary function in mice" ppsx
... and inertance as a function of frequency represents the inertive properties of the gases in the airways The majority of I resides in the central airways bypassed by the tracheal cannula Inertance ... proper insertion of the cannula and to avoid measuring artifacts http://respiratory-research.com/content/8/1/63 The tracheostomy tube can then be attached to a four-way connector, where two ports of ... is inserted into the proximal trachea This enables use of this method in spontaneously breathing as well as in mechanically ventilated mice Orotracheal intubation further offers the opportunity...
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Báo cáo y học: "Pharmacokinetics and lung delivery of PDDS-aerosolized amikacin (NKTR-061) in intubated and mechanically ventilated patients with nosocomial pneumonia" ppsx
... nervous system; FiO2 = fraction of inspired oxygen; IQR = interquartile range; MV = mechanical ventilation; PaO2 = partial pressure of arterial oxygen; VAP = ventilator-associated pneumonia; renal ... 1, 3, 6, 9, 12, 13 and 24 after starting the first aerosol Results are expressed as 3, 6, 9, 12, 13 and 24 after starting the first aerosol medians (interquartile range) Black arrows indicate ... isolates from patients with pneumonia hospitalized in intensive care units Commissioned report North Liberty: The JONES group/JMI Laboratories; 2004 Goldstein I, Wallet F, Nicolas-Robin A, Ferrari...
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Báo cáo khoa học: " Erythropoietin response in critically ill mechanically ventilated patients: a prospective observational study" pot
... oxygen delivery (cardiac output and arterial oxygen saturation) and the overall impact on outcome continues to be evaluated Hebert and coworkers [18] reported the impact of a liberal (threshold ... patients has been reported Further study of therapies directed at treating anemia of critical illness and determining its potential impact on mechanical ventilation outcomes and mortality is warranted ... Acknowledgements Financial support provided by Ortho-Biotech Products, LP through a Cooperative Research and Development Agreement with the Henry M Jackson Foundation The opinions or assertions contained herein...
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Optimal security design and dynamic capital structure in a countinous time agency model DEMARZO & SANNIKOV
... increases only when Wt = W , H is a martingale Because G is bounded, H is a martingale until time τ, so that τ ⎡τ ⎤ G(W0) = H0 =E[Hτ ] = E ⎢ ∫ e − rt g (Wt )dt − k ∫ e − rt dI t + e − rτ L ⎥ ⎢0 ⎥ ⎣ ⎦ ... benefit to the agent of reporting or consuming cash is constant over time, and further, because private savings grow at rate ρ < γ, there is no incentive to delay reporting or consumption In fact, ... equity, it is important that the agent does not receive part of the liquidation payoff Thus, we define inside equity as identical to equity, but with the provision that it is worthless in the event...
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Reproductive senescence and dynamic oviposition behaviour in insects potx
... Without focusing on a particular system, it is most helpful to begin with the simple characterization that clutch size decreases as egg complement decreases Empirical support for this relationship ... evolution of dynamic behaviour (Mangel and Ludwig, 1992; Minkenberg et al., 1992) It is therefore important to consider how dynamic behaviour itself aects egg limitation Empirical evidence that host ... the parasitoid Aphytis lingnanensis (Rosenheim and Rosen, 1991) and a number of ¯y species (Courtney et al., 1990; Fitt, 1990) A convenient description for the dependence of clutch size, c, on...
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Báo cáo hóa học: " Research Article Distributed and Collaborative Node Mobility Management for Dynamic Coverage Improvement in Hybrid Sensor Networks" doc
... exposure, we may convert the problem into a shortest path problem in a time expansion-directed graph by assigning vertices and weights For a given time t = nTr , the vertices of the graph represent ... at the center of a static cell in the grid, there are certain uncovered portions of the corresponding cell Note that this uncovered portion is maximized when a static node is located very close ... nonboundary cell, and the vertices are connected only with the valid candidate cells An illustration of vertex and edge assignments for a Time nTr (9, (n + 1)Tr ) (n + 1)Tr Figure 7: Vertex and edge assignment...
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Báo cáo y học: "Kinematic and dynamic gait compensations in a rat model of lumbar radiculopathy and the effects of tumor necrosis factor-alpha antagonis" ppsx
... Setton1,2 Department of Biomedical Engineering, Duke University, 136 Hudson Hall, Box 90281, Durham, NC, USA Department of Orthopaedic Surgery, Duke University Medical Center, Orthopaedic Clinics, ... Rat Knee Arthritis and Effects of an IL1 Antagonist J Orthop Res 2011, 29:694-703 Allen KD, Adams SB, Setton LA: Evaluating intra-articular drug delivery for the treatment of osteoarthritis in ... hip osteoarthritis [40] Patients with lumbar radiculopathy have also been found to use reduced walking velocities, shorter stride lengths, and increased periods of double limb support [41] The...
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Dynamic performance improvement in boost and buck boost derived power electronic converters
... buck-boost converter (d) Tri-state buck-boost converter (e) Classical flyback converter (f) Tri-state flyback converter (g) Classical full-bridge transformer-isolated boost converter (h) Full-bridge ... converter (i) Classical push-pull isolated boost converter (i) Pushpull isolated tri-state boost converter 95 An existing converter with possible tri-state operation- Cascadebuck-boost converter ... broad classification is as below Dc-dc converters Ac-dc converters Dc-ac converters Ac-ac converters Chapter Introduction In the past, dc-dc converters, popularly known as ‘choppers’ were broadly...
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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt
... was omitted from cDNA synthesis reactions for each sample and RT controls were subjected to qRT -PCR No products were observed in RT reactions using primers for HPRT and mature CB1 mRNA Small amounts ... hypoxanthine ribosyl transferase (HPRT AS: 5¢-CACA GGACTAGAACACCTGC-3¢) The CB1-specific sense primer used in qRT -PCR reactions corresponded to nucleotides +433 to +454 (RT sense 5¢-TCCTTGTAG CAGAGAGCCAGCC-3¢) ... copies of PCR products derived from mature and primary CB1 mRNA and HPRT mRNA were prepared qRT -PCR was performed following the manufacturer’s instructions for LightCycler DNA FastStart SYBRGreen...
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báo cáo hóa học: " The effect of consequent exposure of stress and dermal application of low doses of chlorpyrifos on the expression of glial fibrillary acidic protein in the hippocampus of adult mice" pptx
... calculated Serum corticosterone assay The corticosterone enzyme immunoassay (EIA) kit from Cayman Chemicals (No 10005590) was used This assay used a corticosterone tracer which was a corticosteronecholinesterase ... enzymatic reaction measured in a fluorescence microplate reader at an absorbance of 405 nm was proportional to the amount of corticosteronetracer bound to the well The amount of free corticosterone ... with mouse monoclonal anti-rabbit IgG Corticosterone in the serum sample and the corticosterone tracer provided in the kit compete for limited numbers of corticosteronespecific rabbit anti-serum...
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báo cáo hóa học:" Effects of GnRH agonist treatment on steroidogenesis and folliculogenesis in the ovary of cyclic mice" pot
... treatment of hormone dependent reproductive disorders including infertility, endometriosis, polycystic ovary syndrome (PCOS), precocious puberty, and uterine fibroids etc, whereas others have widely adopted ... on expression of caspase-3 and poly ADP-ribose polymerase (PARP) proteins (Figure 4) Changes in ovarian expression of caspase-3 and poly ADP-ribose polymerase (PARP) proteins are used as marker ... rat and human [22-24] It has been reported Singh and Krishna Journal of Ovarian Research 2010, 3:26 http://www.ovarianresearch.com/content/3/1/26 that short-term GnRH-Ag treatment impairs the...
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Báo cáo hóa học: " Sleep quality in mechanically ventilated patients: comparison between NAVA and PSV modes" doc
... improves this mechanical distortion, whereas PSV worsens this distortion by a tidal volume oscillation (overshoot) during sleep, with a constant patient effort Patient comfort is not only directly ... support must be superior to cmH2O, particularly in COPD if Page of the intention is to compensate work of breathing imposed by the endotracheal tube, ventilator circuit, and patient effort to ... circuit, and patient effort to trigger the demand valve during pressure support [29] A low-pressure support only allows for partial relieve of imposed work of breathing without modifying the work necessary...
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Báo cáo y học: "The influence of the NOD Nss1/Idd5 loci on sialadenitis and gene expression in salivary glands of congenic mice" docx
... versus B10.Q.Nss1/Idd5 B10.Q.Nss1 versus B10.Q.Nss1/Idd5 Array FC QPCR FC QPCR P value Array FC QPCR FC QPCR P value Array FC QPCR FC QPCR P value 2.4 0.03 2.7 4.3 0.01 1.3 1.8 0.25 Egf -3 -5.9 0.04 ... 20× mix) containing two unlabelled PCR primers and one FAM™ dye-labelled TaqMan® MGB probe each The 10 assays used for QPCR validation Available online http://arthritis-research.com/content/9/5/R99 ... selected for QPCR and displays the fold change from the microarray and QPCR as a mean of all individuals in the respective groups All gene expression quantifications obtained in the QPCR were in...
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Antinociceptive effects of morphine and naloxone in mu-opioid receptor knockout mice transfected with the MORS196A gene ppt
... 1Department of Psychiatry, College of Medicine and Hospital, National Cheng Kung University, Tainan, Taiwan, 2Department of Neurosurgery, Tri-Service General Hospital, Taipei, Taiwan, 3Department ... RBL All authors read and approved the final manuscript Acknowledgements This study was supported in parts by grants from the National Science Council (NSC-97-2320-B-016-005-MY3), Taiwan, Republic ... endogenous DOR will inhibit the development of tolerance to that opioid The present data further support our previous findings in ICR mice [3] and suggest that, if the mutant opioid receptor can...
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Báo cáo y học: "Regional characterization of energy metabolism in the brain of normal and MPTP-intoxicated mice using new markers of glucose and phosphate transport" doc
... the heart, the liver or kidneys In addition, these new ligands could help a better understanding of the role of their cognate transporters It is also important to note that these transporters ... sodium-dependent phosphate cotransporter PiT-1 Genesis 2009, 47:858-863 29 Ebert BL, Firth JD, Ratcliffe PJ: Hypoxia and mitochondrial inhibitors regulate expression of glucose transporter-1 via distinct Cis-acting ... has been recently reported to be critical for cell proliferation, a property apparently not shared by PiT2 [13] Several gamma and deltaretroviruses use nutrient transporters as receptors for...
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Báo cáo y học: "Response of the mouse lung transcriptome to welding fume: effects of stainless and mild steel fumes on lung gene expression in A/J and C57BL/6J mice" pot
... confirmed by real time RT- PCR supporting the expression value chosen for IPA Similar corresponding validation was found for LCN2 and MMP12 (Figure 6) Of note, the real time RT- PCR fold induction ... strain Confirmation of microarray gene expression by RT- qPCR Validation of gene expression for CFB, LCN2, MMP12 and SPP1 was done by RT- qPCR from week A/J GMAMS and -SS lung samples (Figure 8) ... were vortexed then sonicated for minute using a Sonifier 450 Cell Disruptor (Branson Ultrasonics, Danbury, CT) Prior to dosing, the samples were vortexed then sonicated for 15 seconds and vortexed...
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Báo cáo y học: "An intranasal selective antisense oligonucleotide impairs lung cyclooxygenase-2 production and improves inflammation, but worsens airway function, in house dust mite sensitive mice" ppsx
... 0.13* 0.365 ± 0.06 a mRNA expression was analyzed by conventional reverse transcriptase -polymerase chain reaction and the expression ratios compared with GAPDH were determined by densitometry ... the manuscript RT participated in the experimental design, planned and performed all of the experiments, and helped in the writing of the manuscript AH, AM, MS, LP, and JRF participated in sample ... In ovalbumin (OVA)-sensitized mice, the results of the blockade of COX-2 activity provide partial support for a protective role of the enzyme The number of inflammatory cells in the airway remains...
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Báo cáo y học: " Different effects of deep inspirations on central and peripheral airways in healthy and allergen-challenged mice" ppt
... oscillation technique) and the single compartment model to characterize the effect of a DI The constant phase model has the capacity to partition the respiratory properties into central and peripheral ... participated in the study design, coordination and helped to draft the manuscript All authors read and approved the final manuscript Acknowledgements This work was supported by the Swedish Heart-Lung ... OVA and http://respiratory-research.com/content/9/1/23 PBS challenged mice also received a shorter s perturbation of forced oscillation (Prime 4), followed by the injection Mice were given one of...
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Báo cáo y học: " Equipment review: Pulmonary uptake and modes of administration of inhaled nitric oxide in mechanically-ventilated patients" ppt
... during pressure support ventilation, intermittent mandatory ventilation, airway pressure release ventilation and pressure-controlled ventilation [3] Its use in pressure-support ventilation, characterized ... concentrations divided by the inspired concentration) is inversely proportional to the alveolar dead space and directly proportional to the volume of normally aerated pulmonary parenchyma in ARDS ... concentration Published with permission [10] the fact that only the perfused part of the ventilated lung parenchyma takes part in the pulmonary uptake of NO [13] It follows that the fluctuation of...
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Báo cáo y học: "Enteral feeding in the critically ill: comparison between the supine and prone positions A prospective crossover study in mechanically ventilated patients" pdf
... for pneumonia, for septic shock, for congestive heart failure and patient for each of the following: pancreatitis, serotonin syndrome and aortic surgery The indication for the prone position ... position (P = 0.59) reports Consecutive mechanically ventilated intensive care patients who were turned to the prone position over a 1-year period were included Enteral feeding started within 24 hours ... Evaluation (APACHE) II scores and associated mortality prediction were calculated within 24 hours of admission The prone position was indicated for an arterial oxygen tension (PaO2)/fractional inspired...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008