poly i c induced inflammatory cell influx is attenuated in tlr3 ko mice
Báo cáo y học: " Lymphocyte apoptosis in murine Pneumocystis pneumonia Xin Shi, Nicole J LeCapitaine, Xiaowen L Rudner, Sanbao " potx
... 5'-AAACTTACACAAGGAGGGTGTTTG-3', 5'-AATGCCTTCTCCATACCAGACG-3', 5'-TTACCGCGAGGCTGAAGACCACCC-3'; mSurvivin, 5'-ATCGCCACCTTCAAGAACTGG-3', 5'TCAGGCTCGTTCTCGGTAGG-3', 5'-ATGAAGCCAGCCT CCGCCATTCGC-3'; ... Health Sciences Center Pneumocystis inoculation Pneumocystis for inoculation was prepared as described earlier using lung homogenates from chronically infected Scid mice [24] In brief, Scid mice chronically ... fluorochromeconjugated Abs specific for murine CD3 (BD), CD4 (Invitrogen) and CD8 (eBioscience), respectively Isotype control antibody staining was used to assist in gating It is difficult to conclusively...
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Báo cáo khoa học: "Density of CD4(+) and CD8(+) T lymphocytes in biopsy samples can be a predictor of pathological response to chemoradiotherapy (CRT) for rectal cancer" ppt
... staining using affinity purified mouse monoclonal antibodies against CD4 (1F6, mIgG1) and CD8 (4B11, mIgG1) (Novocastra, CA) The specificities of these mAbs in immunohistochemistry on paraffin ... were clearly stained in the cell membrane of interstitial infiltrates In most cases, CD4(+) or CD8(+) T cells were evenly distributed in the whole tissue sections, while many cells clustered in ... Hodgkin lymphoma [20,21], which is contrary to the general findings in other tumors [16,22,23] Since anal carcinoma is usually associated with human papilloma virus, specific viral proteins processed...
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Báo cáo hóa học: " Decreased level of recent thymic emigrants in CD4+ and CD8+T cells from CML patients" pdf
... SL, Kolitz J, Schulman P, Lichtman SM, Buchbinder A, Vinciguerra VP, Chiorazzi N, Gregersen PK: Clonal expansion within the CD4+CD57+ and CD8+CD57+ T cell subsets in chronic lymphocytic leukemia ... X, Chen S, Yang L, Chen S, Li Y: Evolution of T -cell clonality in a patient with Ph-negative acute lymphocytic leukemia occurring after interferon and imatinib therapy for Ph-positive chronic ... ± 13.76% in PBMCs from CML patients The sjTRECs levels in PBMCs, CD3+, CD4+ and CD8+ T cells from patients with CML are shown in Figure In comparison with the sjTRECs in healthy individuals (3.76...
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Báo cáo y học: "Heterogeneity of CD4+ and CD8+ memory T cells in localized and generalized Wegener’s granulomatosis." potx
... gradients, cytokine release and cytotoxic activity of distinct T cell populations Detection of the inducible inflammatory chemokine receptors CCR5 and CCR3 on CD45RA+ T cells suggests that CD45RA+ ... differentiation into tissue-specific memory T cells [12,29] Thus, CD4+CD45RA+ and CD8+CD45RA+ T cells expressing the inducible inflammatory chemokine receptors CCR5 or CCR3 as well as CD62L might constitute ... CD45 staining, then analyzed for expression of CD45RO and CCR5 or CCR3 CD8–APC, CD45RO–PE (PE = phycoerythrin), CD45RA–FITC (FITC = fluorescein isothiocyanate), CD45–PerCP (PerCP = peridinin chlorophyll...
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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx
... values in a population at risk for human immunodeficiency virus infection and diagnostic and prognostic application to infected children Pediatr Infect Dis J 1992, 11(8):639-644 Publish with Bio ... Downregulation of cell surface molecules during noncytopathic infection of T cells with human immunodeficiency virus J Virol 1987, 61(12):37413748 Crise B and Rose JK Human immunodeficiency virus type ... the competitor's upon co-amplification Staining with ethidium bromide can be quantitative provided that digital imaging is employed [9] The standard curve plots the log cDNA/competitor band intensity...
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Báo cáo y học: "Genome-wide analysis of primary CD4+ and CD8+ T cell transcriptomes shows evidence for a network of enriched pathways associated with HIV disease" pot
... T cells, reports mainly focused on T cell lines in vitro, except for one study reporting resting CD4+ T cells in viremic versus aviremic HIV+ individuals [16] The limitation of in vitro studies ... amino acids, articulating with the TCA cycle by furnishing substrates Interestingly, this comprehensive and unambiguous expression signature in ex vivo patient-derived T cells is consistent with ... distinct transcriptional profiles in both CD4 + and CD8+ T cells are established early in HIV infection by comparing between early infection, chronic progressive infection, and non-progression...
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Báo cáo y học: " Antibody microarray analysis of cell surface antigens on CD4+ and CD8+ T cells from HIV+ individuals correlates with disease stages" ppt
... individual variability in antigen expression significantly increased expression For HIV-specific CD8+ cells, diminished proliferative capacity results in a memory T cell population with reduced capacity ... Borleffs JC, Boucher CA, de Boer RJ, Miedema F, Hamann D: T -cell division in human immunodeficiency virus (HIV)-1 infection is mainly due to immune activation: a longitudinal analysis in patients ... report of the involvement of intercellular adhesion molecule-1 in syncytia formation and virus infectivity and the increase in its expression on lymphocytes in HIV infection [40] Interestingly, the...
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Báo cáo y học: "Isolated receptor binding domains of HTLV-1 and HTLV-2 envelopes bind Glut-1 on activated CD4+ and CD8+ T cells" pot
... Rathmell JC, Vander Heiden MG, Harris MH, Frauwirth KA, Thompson CB: In the absence of extrinsic signals, nutrient utilization by lymphocytes is insufficient to maintain either cell size or viability ... mAb1418 antibody was selected as being "specific" to Glut-1 According to the manufacturer's indications, this antibody was generated by immunization of mice with a murine cell line transfected with ... and MC participated in the design and validation of antibodies and receptor binding domain constructs as well as binding studies; NM helped to conceive the studies, participated in their design...
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Báo cáo y học: " Interaction between human lung fibroblasts and T-lymphocytes prevents activation of CD4+ cells" pdf
... crucial in the pathogenesis of a number of lung diseases Specifically, an influx of activated T-cells characterizes hypersensitivity pneumonitis and the blockade of T -cell costimulation inhibits ... preservation of IB by interleukin-10 and interleukin-13 J Clin Invest 1997, 100:2443-2448 Boilard E, Surette ME: Anti-CD3 and concanavalin A -induced human T cell proliferation is associated with an increased ... T-lymphocytes and cytokines in sarcoidosis Curr Opin Pulm Med 2002, 8:435-40 Israel-Assayag E, Fournier M, Cormier Y: Blockade of T cell costimulation by CTLA4-Ig inhibits lung inflammation in murine hypersensitivity...
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Xác định sơ bộ giá trị phần trăm, tuyệt đối của tiêu chuẩn quần thể Lympho (T CD3, T CD4, T CD8, B, NK) ở nhóm người bình thường tại thành phố Hồ Chí Minh bằng máy Fascalibur docx
... premixed monoclonal antibodies:CD3 (FITC)/ CD8 (PE)/ CD45 (PerCP)/ CD4 (APC); CD3 (FITC)/ CD16-56 (PE)/ CD45 (PerCP)/ CD19 (APC) After stain, whole blood is passed lyses procedures, wash and final ... đ c phân tách sau máy FASC - Kháng thể BD: + CD3 (FITC)/ CD8 (PE) / CD45 (PerCP) / CD4 (APC) + CD3 (FITC) / CD16,56 (PE) / CD45 (PerCP) / CD19 (APC) - Dung dịch ly gi i hồng c u - Máy FASCalibur ... ng i Iran, Châu Âu, TCD3, TCD4, CD19 thấp V i kh c biệt khiến ng i ta suy nghĩ nh vai trò đáp ứng miễn dịch tự nhiên (thông qua NK) quần thể này? Sự cao tỷ lệ NK c giúp ích cho c thể vi c giúp...
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Báo cáo khoa học: Identification of CD4 and transferrin receptor antibodies by CXCR4 antibody-guided Pathfinder selection pot
... Pathfinder selection, respectively, to isolate scFvs against CXCR4 because of our interest in developing neutralizing antibodies against CXCR4 for potential use in inhibition of HIV-1 infection Pathfinder ... T -cell line), CEMX174 (hybrid human B -cell- T -cell line) and HeLa (human cervical carcinoma) All of the above cell lines were maintained according to the supplier’s recommendations Primary peripheral ... also contribute to the steric hindrance In addition, it is unlikely that the size and genetic complexity of the nonimmune library used in this study limited our ability to isolate CXCR4 scFvs...
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Báo cáo hóa học: "Quantification of newly produced B and T lymphocytes in untreated chronic lymphocytic leukemia patients" ppt
... allophycocyanin-H7 anti-CD4), BioLegend (phycoerythrin antiCD25 and peridin-clorophyll protein-Cy5.5 anti-CCR7), eBioscience (phycoerythrin-Cy7 anti-CD127), and Miltenyi Biotech (allophycocyanin ... amplification of coding joints *Abbreviations: IQR, Interquartile range; KRECs, kappa-deleting recombination excision circles; TRECs, T -cell receptor excision circles supported by the presence in both ... of B -cell divisions in CLL patients While the decreased Ig synthesis in CLL has been previously ascribed to the release of inhibitory cytokines upon cell- cell contact between normal and malignant...
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Báo cáo y học: "Impact of cytokines and T lymphocytes upon osteoclast differentiation and function" pot
... supplied This lineage switch between osteoclasts and dendritic cells has been described in vitro, where cytokine delivery can be precisely defined The situation in vivo is less clear, and the local ... http://arthritis-research.com/content/9/2/103 blockade of GM-CSF production is sufficient to override the inhibitory action of IL-18 In contrast, IL-12 predominantly increases the production of IFN-γ, ... importance of IL-17 and IL-23 in rheumatoid arthritis and other inflammatory diseases has been highlighted in studies using knockout mice IL-17 or IL-23 deficient mice were resistant to Page of (page...
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Báo cáo khoa học:" Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pptx
... mouse cyclin T1 (CycT1), which is an important Figure HIV-1 virus stocks produced in cotton rat cells infect human cells HIV-1 virus stocks produced in cotton rat cells infect human cells Kinetic ... microbicide to prevent viral infection PRO 2000 gel has been shown to inhibit vaginal simian/ human immunodeficiency virus infection in macaques[27], HSV-2 infection in mice[ 28], gonorrhea in ... peripheral blood mononuclear cells (PBMC) Virus inoculation induced a distinct and characteristic HIV-1 antibody response that in some animals We hypothesized that the lack of specific HIV-1 receptors...
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Báo cáo khoa học: " Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pdf
... mouse cyclin T1 (CycT1), which is an important Figure HIV-1 virus stocks produced in cotton rat cells infect human cells HIV-1 virus stocks produced in cotton rat cells infect human cells Kinetic ... microbicide to prevent viral infection PRO 2000 gel has been shown to inhibit vaginal simian/ human immunodeficiency virus infection in macaques[27], HSV-2 infection in mice[ 28], gonorrhea in ... peripheral blood mononuclear cells (PBMC) Virus inoculation induced a distinct and characteristic HIV-1 antibody response that in some animals We hypothesized that the lack of specific HIV-1 receptors...
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Báo cáo sinh học: " Streamlined design of a self-inactivating feline immunodeficiency virus vector for transducing ex vivo dendritic cells and T lymphocytes" ppsx
... Matteucci D, Pistello M, Mazzetti P, Giannecchini S, Isola P, Merico A, Zaccaro L, Rizzuti A, Bendinelli M: AIDS vaccination studies using feline immunodeficiency virus as a model: immunisation with ... mouse models, efficiency at transducing the murine cell line NIH-3T3 was a major guiding criterion in its design as well as in optimizing transduction protocol However, in its original format LA34 ... mostly inactive (selfinactivating [SIN] vectors) Also, these vectors are produced using multiple constructs encoding different components to minimize the risk of generating replicationcompetent viruses...
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Báo cáo khoa học: " Early changes of CD4-positive lymphocytes and NK cells in patients with severe Gram-negative sepsis" ppt
... their modification after triggering by lipopolysaccharide are shown in Figure Discussion The identification of early changes taking place in the clinical setting of a septic host is of prime importance ... 15:148-151 Calandra T, Cohen J, International Sepsis Forum Definition of Infection in the ICU Consensus Conference: The international sepsis forum consensus conference on definitions of infection in ... reach statistical significance compared with controls Our study is the first statistically confirming an early increase of NK cells in severe sepsis Animal studies of experimental infections by...
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Báo cáo y học: "Determinants in HIV-1 Nef for enhancement of virus replication and depletion of CD4+ T lymphocytes in human lymphoid tissue ex vivo" doc
... NAKC signalosome as two distinct protein interaction sites involved in this process Individual mutation of both motifs significantly impaired Nef-mediated CD4+ T lymphocyte depletion, indicating ... lymphoid tissue: correlation of CD4+ T cell depletion and virus syncytium-inducing/non-syncytium-inducing phenotype in histocultures inoculated with laboratory strains and patient isolates of HIV ... [17] Similarly, cell death could be triggered by cell- associated Env via direct contacts with neighboring cells As a consequence of both mechanisms, the number of infected cells is one determining...
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Báo cáo y học: " Effect of chloroquine on reducing HIV-1 replication in vitro and the DC-SIGN mediated transfer of virus to CD4+ T-lymphocytes" pdf
... statistically significant Abbreviations Ab, antibody; CQ, chloroquine; DC, dendritic cell; DCSIGN, DC-pecific ICAM3 grabbing non-intergrin; ECD, extra-cellular domain; HCQ, hydroxychloroquine; R5, CCR5 ... lowering rates of transmission, given the relative inefficiency of infection [35,36] The effect of CQ on DC-SIGN binding in vivo remains to be determined, but if the DC-SIGN binding is indeed ... of HIV-1 with dendritic cell- specific intercellular adhesion molecule-3-grabbing nonintegrin-expressing cells is influenced by gp120 envelope modifications associated with disease progression...
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Báo cáo y học: " Decrease of CD4-lymphocytes and apoptosis of CD14-monocytes are characteristic alterations in sepsis caused by ventilator-associated pneumonia: results from an observational study" ppt
... following combinations were applied: antiCD3(FITC)/CD4(PE), anti-CD3(FITC)/CD8(PE), antiCD3(FITC)/CD(16+56)(PE), anti-CD19(FITC), annexinV(FITC)/CD4(PE)/PI (PC5), and annexin-V(FITC)/antiCD8(PE)/PI(PC5) ... The ACCP/SCCM Consensus Conference Committee, American College of Chest Physicians/Society of Critical Care Medicine: Definitions for sepsis and organ failure and guidelines for the use of innovative ... intraabdominal infection was made in patients with temperature above 38 C or below 36 C, leukocytosis (WBC >12,000 cells/μl) and radiological findings consistent with an intraabdominal infection [15]...
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