physiological roles of parathyroid hormone related protein
Báo cáo y học: " Expression of parathyroid hormone-related protein during immortalization of human peripheral blood mononuclear cells by HTLV-1: Implications for transformation" pps
Ngày tải lên :
13/08/2014, 05:20
... interleukin-1 and parathyroid hormonerelated protein production on adult T-cell leukemia cells in vitro Blood 1995, 86:2257-2267 Dunbar ME, Wysolmerski JJ, Broadus AE: Parathyroid hormonerelated protein: ... ED: Parathyroid hormonerelated protein: hormone and cytokine J Endocrinol 1997, 154(Suppl):S23-S37 Philbrick WM, Wysolmerski JJ, Galbraith S, Holt E, Orloff JJ, Yang KH, et al.: Defining the roles ... interleukin-1, tumor necrosis factor β, parathyroid hormone- related protein (PTHrP), macrophage inflammatory protein- 1α (MIP-1α) and receptor activator of nuclear factor-κB ligand (RANKL) that...
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Báo cáo khoa học: Flexible nets The roles of intrinsic disorder in protein interaction networks potx
Ngày tải lên :
07/03/2014, 21:20
... analysis of the S cerevisiae protein protein interaction network revealed that although proteins with five or fewer links constitute about 93% of the total number of proteins, only about 21% of them ... regions of intrinsic disorder One example of an ordered hub, calmodulin, makes use of a flexible hinge to facilitate binding diversity Each of these classes and their roles in protein protein ... of existing protein protein interaction data can lead to useful information Comparison of protein protein interaction networks across species has significant potential for the study of molecular...
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Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot
Ngày tải lên :
16/03/2014, 06:20
... domain sequences of each protein The chimeric protein composed of the N-terminal domain of hoPL and of the C-terminal domain of hoPLRP2 was named NcC2 Conversely, the other chimeric protein, bearing ... presence of colipase) (500–700 UÆmg)1 for N2 proteins and 6000–8000 UÆmg)1 for Nc proteins) In the presence of bile salts, we observed that the behavior of chimeric proteins is also related to ... presence of NaTDC (data not shown) In the case of NcC2, no inhibition of the lipase activity was observed, as 100% of the lipase activity still remained even at a molar excess of 45 The effect of NcC2...
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Báo cáo y học: " Effect of HIV-1-related protein expression on cardiac and skeletal muscles from transgenic rats" docx
Ngày tải lên :
10/08/2014, 05:21
... myocardial derangements suggesting a predominant role of oxidative stress in HIV-1 -related cardiomyopathies [8] However, the influence of HIV-1 -related protein- induced oxidative stress on specific redox-sensitive ... effect of HIV1 -related protein expression on the glutathione (GSH) anti-oxidant system in heart and plantaris muscles In heart tissues, no effects of the transgene were evident on the levels of GSH ... inductions of catabolic factors by HIV-1 -related proteins represent significant clinical alterations in the evolution of HIV-1 myopathies that are responsible, at least in part, for the establishment of...
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Roles of tuberous sclerosis complex proteins in autophagy and cell death
Ngày tải lên :
10/09/2015, 09:30
... objective of this study is to investigate the roles of TSC proteins in cell death by focusing on the involvement of autophagy and JNK signaling pathway, with the following aims: 1) elucidation of the ... PI3K -related protein kinase PTEN-induced putative kinase protein kinase Polymerase I protein phosphatase peroxisome proliferator-activated receptor gamma proline-rich Akt substrate of 40 kD protein ... and tensin homolog protein tyrosine phosphatases polyvinylidene difluoride quantitative PCR Ras -related GTP-binding protein 7A Ras -related GTPase regulatory associated protein of mTOR Retinoblastoma1-inducible...
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Physiological roles of CIDEs CIDEA deficient mice exhibit lean phenotype and are obesity resistant 6
Ngày tải lên :
17/09/2015, 17:19
... et al., 1997) The uncoupling activity of UCP1 and other uncoupling proteins has been studied extensively in yeast in which the expression of the proteins of interest can be tightly controlled ... identification of the mutant gene underlying the obese phenotype of the ob/ob mouse was made by Zhang et al using positional cloning, and had led to the characterisation of the hormone leptin ... Chapter The original objective of this work was to investigate the physiological role of Cidea, a molecule presumably related to apoptosis Cidea had been shown originally...
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Physiological roles of CIDEs CIDEA deficient mice exhibit lean phenotype and are obesity resistant 5
Ngày tải lên :
17/09/2015, 17:19
... that the physiological roles of the CIDE family members could be related to the regulation of fat metabolism BAT is present in almost all mammals and is essential for the maintenance of body temperature ... deletion of the RII β subunit of protein kinase A, in which the mRNA and protein levels of UCP1 are drastically up-regulated (Cummings et al., 1996) Histologically, brown adipocytes of wild type ... of Cidea-null mice was lower in both basal and isoproterenol-treated tissues compared to that of wild-type mice (Figure 30A) As the complete lipolysis of mol of triacylglycerides yields mol of...
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Physiological roles of CIDEs CIDEA deficient mice exhibit lean phenotype and are obesity resistant 4
Ngày tải lên :
17/09/2015, 17:20
... ES cells of this strain The coat color of 129 strains are agouti (light brown), and the use of blastocysts from C57BL/6J mice, the coat color of which is black, makes the identification of chimeras ... encompassing part of the first intron and the second exon Deletion of this region will result in the disruption of the reading frame and the 67 Chapter generation of a severely truncated Cidea protein ... duplication of the homologous region and an insertion of non-homologous sequences, whereas replacement vector targeting results in the insertion of non-homologous sequences in the middle of the homologous...
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Physiological roles of CIDEs CIDEA deficient mice exhibit lean phenotype and are obesity resistant 3
Ngày tải lên :
17/09/2015, 17:20
... lysates, containing 0.5-1 mg of proteins, were incubated with 1-2 µg of the appropriate antibodies overnight at 4°C with rotation, followed by incubation with protein G PLUS /protein A-agarose (Oncogene ... 500 µl of RIPA buffer and twice with 800 µl of PBS Beads were boiled in 30 µl of 2X protein sample buffer and resolved by SDS-PAGE 2.2.19 Western blot analysis After SDS-PAGE, the resolved proteins ... to wash off phages for hours at room temperature The phage suspension was collected, mixed with 30 µl of chloroform and kept at °C 50 µl of the phage suspension were used to infect ml of host...
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Physiological roles of CIDEs CIDEA deficient mice exhibit lean phenotype and are obesity resistant 2
Ngày tải lên :
17/09/2015, 17:20
... mechanism of regulation of DFF are summarized in Figure Human DFF40 is a basic protein with a pI of 9.3 and is composed of 345 Chapter amino acids, while DFF45 is an acidic protein with a pI of 4.5 ... lipoproteins (HDL), which transport endogenous cholesterol from the tissues to the liver The protein components of lipoproteins are known as apolipoproteins or just apoproteins At least nine apoproteins ... of CIDE family proteins gives us a hint that their physiological role might be more related to regulation of lipid metabolism rather than apoptosis The aim of this project is to resolve the physiological...
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Physiological roles of CIDEs CIDEA deficient mice exhibit lean phenotype and are obesity resistant
Ngày tải lên :
17/09/2015, 17:20
... patterns of the CIDE family members indicate that their in vivo roles could also be related to the regulation of metabolism other than apoptosis In order to resolve the physiological functions of ix ... disruption of the Cideb Gene 63 Figure 12A Genomic DNA sequence of the 5’ region of Cideb gene 64 Figure 12B Genomic DNA sequence of the 3’ region of Cideb gene 65 Figure 13 Inactivation of ... fragmentation, which implies that the mechanism of CIDE function is different from that of DFF45 The project aims at investigating the in vivo physiological roles of CIDEs It was shown that both Cidea...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 1
Ngày tải lên :
12/09/2015, 08:19
... Architecture of mitotic microtubule cytoskeleton 22 1.2.4.2.2 Functions of mitotic microtubule cytoskeleton 23 1.2.5 Self-organization of fission yeast microtubules 1.3 Mia1p as a TACC -related protein ... knock out mutants and epitope tagging of genes 38 2.3.1 Construction of knock out mutants 38 2.3.2 Construction of epitope tagging of genes 39 2.3.3 Generation of cells overexpressing Mia1p 39 2.4 ... Transformation of E.coli by electroporation 36 2.2.3 LiAc transformation of S pombe 36 2.2.4 Mia1p/Microtubule association experiments 37 2.2.5 Extraction of S pombe genomic DNA 37 2.3 Construction of knock...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 2
Ngày tải lên :
12/09/2015, 08:19
... Recently, a novel family of centrosomal proteins, the transforming acidic coiled-coil -related (TACC) proteins, has been identified in a wide variety of organisms These closely related proteins are enriched ... that at least two proteins are involved in this process One of them is the minus-end-directed protein Klp2p 1.2.3.1 Minus-end-directed motor protein Klp2p Klp2p is a kinesin of the KAR3 subfamily ... 2007), thus the plus end of a newly nucleated microtubule serves as a cargo of Klp2p to be delivered to the minus end of mother microtubule In the absence of Klp2p, sliding of newly nucleated microtubules...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 3
Ngày tải lên :
12/09/2015, 08:19
... DNA was stored at -20oC 2.3 Construction of knock out mutants and epitope tagging of genes 2.3.1 Construction of knock out mutants To create null mutant of S pombe strains, gene coding regions ... determination of the genomic DNA 38 2.3.2 Construction of epitope tagging of genes All chromosomally tagged genes were created through homologous recombination Green fluorescent protein (GFP) ... resuspend in 100μl of LiAc/TE [make 10 times dilution from 10X LiAc (1M LiAc, ph 7.5) and 10X TE (0.1M Tris-HCl, 0.01M EDTA, ph 7.5) solutions] 36 Add 2μl of carrier DNA and 2-10μl of plasmid DNA...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 4
Ngày tải lên :
12/09/2015, 08:19
... Microtubule-associated protein Mia1p binds microtubules and is a component of S pombe MTOCs (A) Shown is the maximum projection of the z-stack obtained by epifluorescence imaging of interphase cells ... Plot of SPB position over time in wild type cell expressing α-tubulin-GFP and Sid2p-GFP Some extreme positions of the SPB (indicated as 1-4) are shown on the right of the graph (2) Plot of SPB ... found that in the absence of Mia1p-interacting protein of the MAP215 family, Alp14p, microtubules remained attached to the SPBs in the time-lapse sequence analysis of α-tubulin-GFP Sid2p-GFP...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 5
Ngày tải lên :
12/09/2015, 08:19
... represent the sites of attachment of microtubule bundles to the NE One of the more obvious physiological functions for these structures could be ensuring the proper centering of nuclei (Tran et ... Mia1p and Alp14p Mia1p belongs to a family of centrosomal proteins, the transforming acidic coiled-coil -related (TACC) proteins characterized by the presence of a C-terminal TACC domain (Gergely, ... contributions of these two proteins in organizing interphase microtubule arrays 4.5.3 Lack of Mia1p and Alp14p result in failure of iMTOCs establishment Another striking aspect of Mia1p function...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 6
Ngày tải lên :
12/09/2015, 08:19
... (1998) The TOGp protein is a new human microtubule-associated protein homologous to the Xenopus XMAP215 J Cell Sci 111 ( Pt 10), 1371-1383 15 Cullen, C.F., and Ohkura, H (2001) Msps protein is localized ... both centriolar and pericentriolar material proteins Dev Cell 7, 815-829 19 Demeter, J., Morphew, M., and Sazer, S (1995) A mutation in the RCC1 -related protein pim1 results in nuclear envelope ... Dynamics of interphase microtubules in Schizosaccharomyces pombe Curr Biol 10, 766-775 23 Drummond, D.R., and Hagan, I.M (1998) Mutations in the bimC box of Cut7 indicate divergence of regulation...
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Tài liệu Báo cáo khoa học: Angiopoietin-like proteins: emerging targets for treatment of obesity and related metabolic diseases pptx
Ngày tải lên :
14/02/2014, 22:20
... ANGPTL2 causes inflammation of adipose tissue in obesity and related insulin resistance [15] Here, we focus on the roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases, ... NG & Koh GY (1999) Molecular cloning, expression, and characterization of angiopoietin -related protein Angiopoietin -related protein induces endothelial cell sprouting J Biol Chem 274, 26523–26528 ... etc) Induction Fig Schematic diagram showing the roles of ANGPTL2 and ANGPTL6 ⁄ AGF in obesity and related metabolic diseases The expression of ANGPTL2 and ANGPTL6 ⁄ AGF is induced in obese conditions...
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Tài liệu Báo cáo khoa học: Autolytic activity of human calpain 7 is enhanced by ESCRT-III-related protein IST1 through MIT–MIM interaction pptx
Ngày tải lên :
18/02/2014, 04:20
... domain-containing proteins have been shown to bind endosomal sorting complex required for transport (ESCRT)-III proteins and their related proteins [21–23] Fig Schematic representations of calpain and ... further Estimation of cleavage sites in mGFP–calpain Direct interaction between recombinant calpain 7MIT and GST–IST1 proteins The use of cleared lysates of HEK293T cells for all of the experiments ... pefabloc (serine protease inhibitor) Effects of ESCRT -related proteins on autolysis of mGFP–calpain in vivo Next, we examined whether IST1 affects the generation of 30 K in vivo mGFP–calpain was coexpressed...
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Tài liệu Báo cáo khoa học: Identification of membrane-bound serine proteinase matriptase as processing enzyme of insulin-like growth factor binding protein-related protein-1 (IGFBP-rP1/angiomodulin/mac25) doc
Ngày tải lên :
19/02/2014, 07:20
... high level of proteinase(s) responsible for the processing of IGFBP-rP1 To determine the class of the IGFBP-rP1-cleaving proteinase, OVISE cells were cultured in the presence of various proteinase ... #88 Assay of IGFBP-rP1-cleaving activity after SDS/PAGE separation of proteinases IGFBP-rP1-cleaving proteinases present in the membrane fraction of OVISE cells and conditioned medium of DLD-1 ... the cleaved form (25 kDa) of IGFBP-rP1 The lower panel shows protein staining patterns of conditioned media Bars indicate the molecular size in kDa Analysis of serine proteinases secreted by various...
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