phenotypic and functional analyses of cd34neg hematopoietic precursos from mobilized peripheral blood
Báo cáo khoa học: "Phenotypic and functional analysis of bovine γδ lymphocytes" pps
... Determination of the antigenic phenotype and frequency of subsets of WC1+ and WC1- γδ T cells in peripheral blood and lymphoid tissues: i) Flow cytometric analysis: Analysis of the tissue distribution of ... expression of WC1N3 and WC1-N4 isoforms and expression of families of the γδ TCR that express determinants TCR1-N6, TCR1N7, or TCR1-N6 and -N7 Only a subset of WC1- γδ T cells expressed a form of TCR1 ... pattern of expression of TCR1N6+ and TCR1-N7+ cells also suggested expansion of the WC1+ population of γδ T cells, in the course of evolution, included selective usage of a subset of TCR1 Vγ, Jγ, and...
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Báo cáo y học: "Phenotypic and functional characterisation of CD4+ memory T cells homing to the joints in juvenile idiopathic arthritis" pps
... expression of CCR7, CCR5 and CXCR3 on SF and peripheral blood (PB) memory CD4+ T cells from patients with JIA, chemoR257 PB and SF mononuclear cells (MNC) were isolated from heparinised blood and SF ... http://arthritis-research.com/content/7/2/R256 Table Clinical and laboratory features of patients with juvenile idiopathic arthritis at the time of phenotypic and functional studies of peripheral blood and synovial fluid lymphocytes ... taxis of the latter cells to the ligands of these receptors, and the distribution of cells positive for CCR7, CCR5 and CXCR3 in the inflamed synovium Methods Patients Immunophenotypic and functional...
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Báo cáo y học: "Phenotypic and functional characterization of switch memory B cells from patients with oligoarticular juvenile idiopathic arthritis" pps
... Flow cytometry of B-cell subsets in synovial fluid (SF) and peripheral blood (PB) from juvenile idiopathic arthritis (JIA) patients Mononuclear cells patients (MNCs) from SF and PB of 25 JIA patients, ... expanded in the peripheral blood from SLE patients, and this expansion correlated with high disease activity and high titers of disease-specific autoantibodies [18] In contrast, the frequency of ... of JIA by upregulating the expression of co-stimulatory molecules such as CD80 and CD86 and presenting antigens to T cells [9] With this background, we here address the immunophenotypic and functional...
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báo cáo khoa học: " Molecular and functional analyses of COPT/Ctrtype copper transporter-like gene family in rice" pdf
... BamHI/EcoRI sites of p413GPD(+His) vector and the cDNAs of COPT4 and COPT7 were subcloned into the SpeI/EcoRI sites of p413GPD(+His) separately [39] The cDNA of S cerevisiae Ctr1 was amplified from yeast ... expression of rice COPT1 and COPT5 was influenced by Cu; COPT1 and COPT5 were induced by Cu deficiency and suppressed by overdose of Cu in both shoot and root tissues [29] The expression of other ... expression of COPTs (Figure 8a) Mn deficiency induced COPT1 in root and COPT3 and COPT7 in shoot, and slightly suppressed COPT2 and COPT4 in root Zn deficiency induced COPT1, COPT5, and COPT7 and slightly...
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Báo cáo y học: "Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus" pot
... higher levels of DC-SIGN, CD123, and CD40 but lower levels of CD11c and HLA-DR than did controls (d, e) Immature and mature mDCs from normal controls and patients with SLE SLE immature and mature ... BDCA-2 and CD123, whereas immature and mature mDCs expressed no or low levels of these molecules (b, c) Immature FLDCs and mature FLDCs from normal controls and patients with SLE SLE immature and ... controls They were aged from 23~60 (mean, 45 ± 11) years Page of 12 Generation of DCs from BM cultures and analysis of control BMDCs Previous reports showed that the administration of FL to mouse BMCs...
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Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx
... fully active alpha isoform and other variants, we conducted a series of cotransfection experiments and compared ligand-induced accumulation of cAMP (Fig 3) Although the level of cAMP accumulated ... differential expression of isoforms of CRFR1 to precisely couple selectively activated phenotypic targets, we performed molecular characterization of newly described CRFR1 isoforms First, we tested ... boxes, insertion of a cryptic exon (B) The structure of constructs used for functional assays (C) The structure of constructs used for Western blotting In general the majority of our isoforms were...
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Báo cáo y học: " Aberrant splicing of the hRasGRP4 transcript and decreased levels of this signaling protein in the peripheral blood mononuclear cells in a subset of patients with rheumatoid arthritis" pdf
... acquisition of the data and draft of the manuscript HT helped collection and acquisition of the data and draft of the manuscript TA and TK was involved in the interpretation and design of the study, and ... expression of hRasGRP4 in the peripheral blood evaluated by immunoblotting A Immunoblotting of peripheral blood mononuclear cells from patients with RA and healthy controls Lysates were prepared from peripheral ... Committee of Hokkaido University Graduate School of Medicine, and informed consent was obtained from each subject Cell separation PBMCs were collected from ~10 ml of the peripheral blood drawn from...
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Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt
... studies of the structure and binding sites of enzymes Inhibitor binding to ribonucleases J Mol Biol 45, 491511 33 Ui N (1971) Isoelectric points and conformation of proteins I Effect of urea ... Fc| RhFo, where Fo and Fc are the observed and calculated structure factor amplitudes of reection h, respectively Rfree is equal to Rcryst for a randomly selected 5.0% subset of reections not used ... structural interactions of a nucleic acid with a protein Conclusions The functional and structural studies presented herein demonstrate the attributes of N-acylsulfonamidyl and sulfonimidyl groups...
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báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
... previously described [17] Culture of peripheral blood monocytes and CD34+ progenitors Monocytes were generated from normal volonteers' monocytes after elutriation of peripheral blood according to the french ... (CD123) and the CD4 [18] Moreover, as a hallmark of pDC, these cells are BDCA2 and BDCA4 (type II C lectin)-positive and CD11c- and CD1a-negative Trypan blue exclusion and cell counting of LV and ... Optimization of gene transfer into primitive human hematopoietic cells of granulocyte-colony stimulating factor -mobilized peripheral blood using low-dose cytokines and comparison of a gibbon ape...
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Tài liệu Physical health and functional ability of an elderly, population in Sri, Lanka doc
... of physical performance for assessing the functioning of upper and lower extremities were carried out, using standard procedures These included: semi tandem stand, full tandem stand, rising from ... 84% in Indonesia and 62% in Thailand (10) It was D N Fernando and R de A Seneviratna 14 Table Self-assessed "health status" by some psychosocial factors and indicators of use of health services ... American Journal of Public Health 1981; 71(11): 1211-1216 D N Fernando and R de A Seneviratna Fries J F Aging, natural death and the compression of morbidity Tlie New England Journal of Medicine 1980;...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... hood domain is mainly built up by residues from the C-terminus and consists of a two-stranded anti-parallel b-sheet composed of b2 and b9 and an a-helix from the C-terminus (Fig 1A) The two subunits ... elucidation of the structure of PRTFDC1 and the definition of its ligand binding specificity from a large metabolome library provides an initial start point for defining the molecular function of PRTFDC1 ... side chain of Lys173 interacts with both O6 and N7 (Fig 1C) The 2¢OH of the ribose is hydrogen bonded to the main chain and the side chain of Asp142 (Fig 1C) Both hydroxyl groups of the ribose...
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Tài liệu Báo cáo khoa học: Interleukin-1-inducible MCPIP protein has structural and functional properties of RNase and participates in degradation of IL-1b mRNA doc
... clarify the dynamics and features of this protein Role of MCPIP in regulation of the endogenous IL-1b transcript level After identification of a PIN domain in the MCPIP by use of bioinformatics, ... overexpression and 25% for siRNA), the overall conclusion from the experiment with fibroblasts is that MCPIP regulates the amount of IL-1b mRNA Involvement of PIN domain of MCPIP in the stability of IL-1b ... lanes 2,3 and 4, lower band) The levels of mutant and wild-type MCPIP mRNAs were higher when these vectors were co-transfected with a vector overexpressing IL-1b (Fig 5, lanes and 4, upper bands)...
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Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt
... Asp70, and one from the backbone N of Gly63, and the phosphate group forming hydrogen bonds from O1 to Arg55, from O2 to the backbone N and the side chain of Thr138, and from O3 to the backbone N of ... bonds from N3 to the backbone O of Phe133, and from O4 to the backbone N and the side chain of Thr131, with the ribose moiety anchored by two hydrogen bonds, one from 2¢-OH to the side chain of ... D and E + F), (b) a few hydrogen bonds, and a hydrophobic interaction between A + C, B + E and D + F, and (c) electrostatic forces in the central channel of the hexamer between B, C and F, and...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... plausibly involved in DNA recognition and binding and is positioned left of the active site [14] Rep245 is both capable of de novo synthesis of DNA primers and of elongating them Long DNA extension ... concentration of Rep245 prepared in 10 mm sodium phosphate (pH 7.4) was lm CD spectra were recorded at 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal ... nature of the acid residues within the active site of Rep245 The carboxylate triad of Rep245 including the D101, D103 and D166 motif is similar to the triads of X family DNA polymerases and terminal...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... Kirschbaum B & Loffler M (1996) Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified ... mutation of deoxyribonucleoside kinase L Egeblad-Welin et al Mutants of substrate-binding residues: Y70 and Q81 Y70W showed an increase in Km values for both dThd and dCyd, of approximately 200- and ... value for dThd and dCyd, of approximately 200- and 100-fold, respectively The kcat values were only decreased approximately twofold for both substrates Phosphorylation of nucleosides and nucleoside...
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Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf
... describes the cloning and functional characterization of an AK gene from a genomic library of L donovani as well as its expression and molecular characterization Materials and methods Materials ... after induction of the culture with IPTG, which matched the molecular mass predicted from the amino acid sequence of AK2 protein The purification and functional folding of L donovani AK from the E ... growth curve of promastigotes Functional characterization of recombinant AK The recombinant protein purified from the inclusion bodies and refolded in the presence of dithiothreitol, MgSO4 and KCl...
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Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt
... consists of a large, rapid and transient response that is typical of Ca2+-dependent Cl– channels activated after stimulation of PLC and the subsequent InsP3-dependent mobilization of Ca2+ from intracellular ... value and n represents the number of oocytes tested for each condition TRHRs and its similarity to most of the regions of the mouse, rat and fish TRHR2 indicate that xTRHR2 is a member of the ... Diagram of the xTRHR cDNA, PCR primers and PCR products (A) Amplification of the middle region of the xTRHR cDNA by nested PCR The relative positions of the degenerated primers TRHR1, TRHR2, TRHR3, and...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx
... of hydrogen, modification of bonds, potentials of forcefield, and fixation of heavy atom, backbone, and Ca, were performed for molecular mechanics calculations and then the energy minimization of ... possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat approach of LPL [21,34] support ... domain of apoCII interacts with the interface of the N- and C-terminal domains of LPL and part of the lid surface [32] Because the functional importance of LPL’s C-terminal domain is increasingly...
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Báo cáo khoa học: Structural and functional aspects of unique type IV secretory components in the Helicobacter pylori cag-pathogenicity island potx
... a-helices and a final b-strand, F, which protrudes from the core of the monomer and runs anti-parallel to the same strand of a second monomer, allowing for the formation of the dimer The surface of interaction ... glutamate and aspartate residues confined to the portion of the molecule involving a-helix A, the nearby loop, the first and last turns of helices E and F, and the C-terminus helices I and J In ... structure of only three of these unique components (CagZ, CagS and CagD) is now available, along with Caga ATPase The lack of a clear picture of the biological function and organization of some...
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Báo cáo khoa học: Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27 ppt
... FEBS 3267 Analyses of novel Thermus competence proteins J Rumszauer et al N-terminal domain of PilQ, and the colocalization of PilQ with PilW in inner and outer membranes and the results from the ... to the C-termini of other members of the secretion family, such as PilQ of Myxococcus xanthus [28], ExeD of Aeromonas salmonicida [29], PilQ of P aeruginosa [25,30], and PilQ of N gonorrhoeae ... PilA4 and PilQ are individually essential for transformation and piliation [7,8] In contrast to PilQ and PilA4, an individual role of PilM, PilN, PilO and PilW in natural transformation and piliation...
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