... Nagai S, Miki H, Ito I, Izumi T: Cytotoxic effects of cigarette smoke extract on an alveolar type II cell-derived cell line Am J Physiol Lung Cell Mol Physiol 2001, 281(2):L509-516 Rahman I, Mulier...
... through the whole course of the project iii TABLE OF CONTENTS PREFACE Page i ACKNOWLEDGEMENTS ii- iii TABLE OF CONTENTS iv-vi SUMMARY vii-ix LIST OF FIGURES x-xiii CHAPTER INTRODUCTION 1.1 Background ... lines (- - -) are those for (i) PLA, a common tissueengineering polymer (ii) artery-derived acellularized matrix; and (iii) matrigel [Disher, 2005] An interplay of physical and biochemical signals ... lines (- - -) are those for (i) PLA, a common tissue-engineering polymer (ii) artery-derived acellularized matrix; and (iii) matrigel [Discher, 2005] 2.4 Effect of substrate stiffness on cell growth...
... monocytes were selected from PBMC by magnetic cell sorting using respectively the NK isolation kit II, the CD4 T cell isolation kit and CD14 microbeads (Miltenyi Biotec, Auburn, CA, USA) according ... and monocytes Flow cytometry and cell surface staining Flow cytometry was performed using a LSR -II cytometer, BD biosciences, San Jose, CA, USA Data treatment and analysis were performed using ... Progression of cellular mean fluorescence intensity was analysed using modelling software (SAAMII, v1.2, University of Washington) Statistical analysis Statistical analyses were carried out using...
... foetal body weight was decreased [18], minor skeleton variation with the exception of short rib XIII in rats [19] Figure SCE in the control group The black arrow indicated SCE in human chromosome...
... hypotheses: (I) these drugs affect osteogenic cell proliferation, (II) their effects on cell proliferation are patient-specific, and (III) these drugs enhance or reduce each other's effects when combined ... number in the culture wells was assessed colorimetrically using the Roche Cell proliferation Kit II (Roche, Mannheim, Germany) This method is as sensitive as radioactive assay with a significantly ... combination drug therapy on Osteo- Busti AJ, Hooper JS, Amaya CJ, Kazi S: Effects of perioperative antiinflammatory and immunomodulating therapy on surgical wound healing Pharmacotherapy 2005, 25(11):1566-91...
... directed to chicken collagen type II (CII) by enzyme-linked immunosorbent assay as described earlier [31] For evaluation of delayed-type hypersensitivity (DTH) reactivity, CII/complete Freund’s adjuvant ... rheumatoid arthritis, depends on both CII-specific T cells and antibody responses against CII [43,44] Both of these specific immune responses against CII remained unaffected upon transfer of ... was also reflected in cellular and humoral autoimmune responses, DTH and total anti-CII IgG were analyzed Anti-CII IgG titers were similar between MSC-treated and PBS-treated mice (Figure 4c) In...
... mice with hypoxia-induced pulmonary hypertension Biochem Biophys Res Commun 2006, 345(4):1565-72 Ii M, Hoshiga M, Fukui R, Negoro N, Nakakoji T, Nishiguchi F, Kohbayashi E, Ishihara T, Hanafusa...
... allergic symptoms in early childhood Clin Exp Allergy 1999, 29:1042-1048 Pesonen M, Kallio MJ, Siimes MA, Elg P, Bjorksten F, Ranki A: Cord serum immunoglobulin E as a risk factor for allergic...
... The third exception are several tRNA genes, demonstrating that Myc can also stimulate polymerase III-dependent transcription (Gomez-Roman et al Fig Genetic targets of Myc in cell proliferation ... effect is direct and not mediated by activation of protein-coding genes that regulate polymerase III function; however, the mechanism of activation has not been clarified Myc proteins also act as ... highly conserved domain in the amino-terminus of Myc proteins (MycboxII), and Myc controls acetylation of its target genes in a MycboxII-dependent manner Mutations in this domain impair both activation...
... p27KIP1DPSD-AMC substrate was not increased over the basal level The effect of the topoisomerase II inhibitor, etoposide, was then analysed in order to compare two quite distinct routes to apoptosis...
... Briefly, a 354-base pair fragment amplified from the human genomic c-myc gene [20] was inserted into pCRII-TOPO (Invitrogen Ltd, Paisley, UK) Equal counts of 32P-labeled RNA ( · 106 c.p.m.) were hybridized ... RNA was also hybridized with unlabeled antisense GAPDH mRNA synthesized from a SacI-linearized pCRII-TOPO vector, containing a 788-base pair fragment amplified from the human genomic GAPDH gene [20] ... dependent signaling pathways J Biol Chem 276, 2015–2022 Shigematsu, S., Yamauchi, K., Nakajima, K., Iijima, S., Aizawa, T & Hashizume, K.D (1999) Glucose and insulin stimulate migration and tubular...
... recombination (A) A, A II, Bg, BglII; BH, BamHI; E, EcoRI; Sm, SmaI; Ss, Sse8387I (B) Southern blot analysis using the probes described in (A) Left panel: digested with A II and detected by probe ... sequences are described in Table S1 cDNA templates for probes were synthesized by SuperScriptII ⁄ III (Invitrogen) according to the manufacturer’s instructions Cell culture ES cells were cultured ... relevant primers (Table S1) The resulting cDNA fragment was digested with HindIII and SlaI, and then subcloned into pBluescript II (Stratagene, La Jolla, CA, USA) and sequenced For the PTB expression...
... Government of India to D.P The authors thank Sophisticated Analytical Instrument Facility (SAIF), IIT Bombay for use of the electron microscopy facility and Renu Mohan and K Rathinasamy for critical...
... containing a polycarbonate filter with lm pores Serumfree DMEM with chemoattractant [1 lM angiotensin II (AngII)] was added to the lower compartment while inhibitors were added to the upper compartment ... had been stimulated with either AngII or PGE2 It was observed that both cytosolic and microsomal arg-mART activity were increased transiently following AngII (1 lM) stimulation (Fig 3A), with ... 270) 105 Fig Activation of mono-ADP-ribosyltransferase by angiotensin II and prostaglandin E2 Quiescent SMC were treated with AngII (1 lM) or PGE2 (1 lM) over 120 and subcellular fractions prepared...
... coli Biochem Biophys Acta 287, 282–291 Funatsu, G & Wittmann, H.G (1972) Ribosomal proteins XXXIII Location of amino acid replacements in protein S12 isolated from Escherichia coli mutants resistant ... mutation J Mol Biol 39, 95–112 Funatsu, G., Nierhaus, K & Wittmann, H.G (1972) Ribosomal proteins XXXVII Determination of allele types and amino acid exchanges in protein S12 of three streptomycin-resistant ... 1162–1164 39 Spirin, A.S (1991) Cell-free protein synthesis bioreactor In Frontiers in Bioprocessing II (Todd, P., Sikdar, S.K & Beer, M., eds), pp 31–43 American Chemical Society, Washington, DC 40...