... taken by inviting the best thoughts of some ofthecountry' s best minds on the subject of "The Morals of Trade." What better platform for the expression of such ideas than that furnished bythe ... none the less, that there clearly belongs to this great calling a series of duties of a public nature, some of them imposed bythe laws ofthe land, and others inherent in the very nature ofthe ... before to the whirl of industry and the rush and excitement ofthe market-place This, of all ages, is the age ofthe business man The heroic times when warfare was the chief concern of nations,...
... that the meaning ofthe word is The main goal ofthe study is to investigate the semantic and not determined by itself but byits relations with other linguistic and pragmatic properties ofthe ... situations, by which the polysemy ofthe adjective is displayed semantic features ofthe adjective Warm through analyzing its grammatical structures It should be noted that the variety ofthe meaning of ... shows their and Heidi Weber [28, p 254] say that semantic features are the relationship to one another.” smallest units of meaning in a word The meaning of a word may be 2.2.2 The Notions and the...
... binding affinity by 100-fold and the lifespan ofthe complex by 10-fold, as judged by koff The physiological chaperone cycle probably consists ofthe rapid binding ofthe N-terminus ofthe partner, ... hypothesize that the protonated state ofthe chaperone initiates this cycle, whereas the deprotonated state occurs upon completion ofthe maturation process ofthe partner The nature ofthe signal ... enthalpy, independent ofthe buffer used in the experiment, and another term representing the contribution ofthe proton ionization ofthe buffer, which is multiplied bythe number of protons that...
... in part by a subcontract fromTheOf ce of Biomass Program, within the DOE Of ce of Energy Efficiency and Renewable Energy Tom Desmet holds a fellowship ofthe Institute for the Promotion of Innovation ... composed of at least four subsites () ⁄ + 2) The preferential release of GCNP fromthe chromogenic substrates excludes the use of direct spectrophotometric assays The strong contribution of all ... completely block the active site cleft of Cel74A at subsite + 2, as its end-products of xyloglucan hydrolysis have a backbone degree of polymerization of four instead of two Furthermore, its preference...
... account by normalizing the FT-IR difference spectra on the difference signal ofthe sample in the UV/VIS at 602 nm Redox titrations The redox-dependent absorbance changes ofthe caa3oxidase from T thermophilus ... c, with contributions fromthe formyl groups and fromthe geranyl side chain expected from heme a and a3 In addition to the signals ofthe hemes, the reorganization ofthe polypeptide backbone ... which show deviations to the centrally discussed oxidases The study of these aberrant systems is important to understand the principles of these enzymes The cytochrome c oxidase from T thermophilus...
... independent ofits phosphorylation status (Fig 7C) The specificity ofthe SenR–up-hbpS2 interaction was further corroborated by competition using constant amounts of SenR and increasing amounts of unlabeled ... using constant amounts of SenR and increasing amounts of unlabeled up-hbpS verified the specificity ofthe SenR–up-hbpS1 interaction (Fig 5C, third box from left) Taken together, these data revealed ... together, these data confirm the specificity ofthe SenR-binding sites and corroborate the assumption that phosphorylation of SenR by SenSP alters its DNA-binding characteristics Discussion The...
... efficient nucleophilic attack ofthe substrate ester bond bythe serine alcoholate The shape ofthe titration curve ofthe catalytic histidine shows an apparent pKa value ofthe essential histidine ... presence of Arg213 along with the proximity ofthe charge relay Asp260 within the active site could be responsible for the modification ofthe catalytic properties ofthe catalytic histidine The strength ... as estimated by Electrospray mass spectrometry is due to the presence, at the N-terminus, ofthe His6-tag and the additional 21 residues fromthe expression plasmid The annotation of Rv1399c as...
... bind to all ofthe tRNA binding sites on the ribosome (i.e A, P and E sites) [13,14] As expected, the addition of SF to the system resulted in pppGpp synthesis (Fig 2) The speed of synthesis, calculated ... template, the activity ofthe enzyme is similar to that in the absence of tRNA This is not surprising because the template is needed to position tRNAs on the ribosome [26] The activity ofthe poly(U)-dependent ... P-sites ofthe 30S and 50S subunits and the tRNAPhe bound in the A-site ofthe 30S and 50S subunit [28] Moreover, a tRNAMet was present in the 50S f E-site The E-site bound tRNA did not affect the...
... locations ofthe a helical secondary structures derived from these data are indicated by bars at the bottom Fig Alignment of c subunits from E coli and P modestum and location ofthe a helical ... is due to the interaction ofthe monomer with the artificial environment lacking the stabilizing protein/protein contacts within the ring The undecameric c ring ofthe ATP synthase from Ilyobacter ... determination by cryo-transmission electron microscopy [20] All c subunits ofthe ring show the hairpin-like folding and the structure of all monomeric units appears to be the same Hence, the structural...
... 3–6 mgÆL)1 of culture From these data, it was concluded that the full-length form of GluV8 could be recovered quantitatively bythe suppression of self-degradation, either bythe use ofthe GluSE ... processed by thermolysin, its overall structure does not appear to be altered fromthe active form Therefore, to elucidate the mechanism of suppression ofthe protease activity and the alteration in the ... for the proteolytic entity This is further indicated bythe finding that the last four residues ofthe propeptide of GluSE, which are completely different from those of GluV8, are sufficient for the...
... also shown that the oxidation of geraniol is one ofthe key steps in the production of neral [12] The low amountofthe enzyme may be due to its specific role in alarm pheromone biosynthesis Citral, ... However, the subunit structure of Car lactis GeDH was quite different from that of other ADHs As far as we know, there is only one other example of a monomeric ADH with activity, isolated fromthe ... sequences of Car lactis GeDH and other ADHs by minimum evolution criterion The scale ofthe distances is shown under the tree The tree is separated into three groups (group 1, ADHs from animals...
... such bythe presence of stromules [20], indicated bythe arrows in the higher magnification images The scale bar is 50 lm in the images on the left, and 10 lm in the higher magnification images from ... three constructs From these experiments we can conclude that the presence ofthe GFP as passenger protein, rather than the mature SSU protein, did not affect the ability ofthe SSU transit peptide ... (GFP) from Aequorea victoria [15], and the phosphorylation site was mutated After transformation ofthe construct into plant cells by particle bombardment, the targeting ofthe GFP bythe transit...
... during the structure solution ofthe native enzyme Family contains both CBHs and endoglucanases The structure of CBHs is distinguished from that of endoglucanases bythe presence of loops of polypeptide ... which runs the length ofthe concave sheet, into which the cellulose substrate can be accommodated The b-sandwich represents the characteristic fold of GH7 and is also the fold ofthe legume-lectin ... hold the b-sandwich in position With the exception of four a-helices and two pairs of short b-strands, the rest ofthe protein consists almost entirely of loops connecting the b-strands The loops...
... determined by comparing the retention times of FAMEs (fatty acid methyl esters) from glycolipids to the standards in GC-MS analysis The methyl esters were prepared by treatment ofthe glycolipids ... (m/z 1096) (Fig 1B) The lack of adequate detection of N-acyl lipids was due to the relative stability ofthe amide bond under methanolysis conditions The significant amountof hydroxy fatty acids ... negative peaks in the HSQC experiment Table NMR data ofthe tetraglycosyl glycerol derived fromthe major glycolipid from Meiothermus taiwanensis ATCC BAA-400 In p.p.m fromthe HSQC spectrum...
... bacteria, the 14 subunits ofthe complex I are grouped into three modules: seven subunits form the membrane subcomplex, four subunits form the connecting subcomplex and the last three subunits form the ... et al ofthe ndh genes is much higher in BS chloroplasts, and elevated amounts ofthe Ndh complex have been found in these plastids [18] The function ofthe Ndh complex is still a matter of debate ... mass and the number of subunits ofthe Ndh complex obtained by BN ⁄ PAGE (at least 14 subunits), colorless native PAGE (CN ⁄ PAGE; in which separation ofthe protein complexes is based on their...
... contribution ofthe e subunit to the stability ofthe rotor in these cases is not yet clear Does the interaction ofthe c ring with the c and e subunits have anything to with the regulation of enzyme ... changes in the HSQC spectrum ofthe cÂe complex by mutating the cE204 residue to Gln Inset in (B) indicates the changes in the selected areas ofthe HSQC spectrum ofthe cÂe complex imposed bythe addition ... to empty chip), allowing the elimination ofthe latter by baseline correction (see below) As a result ofthe location ofthe His tag on the very top ofthe c subunit, the immobilized cÂe complexes...
... share ofthe euro in the bond portfolios of large international fund managers, on the one hand, and the rising share ofthe euro in the stock of international bonds, on the other Looking ahead, the ... ofthe roadshow to the execution ofthe final sale, through a bank syndicate, for about one-fifth ofthe issues Banks from Switzerland had a 15% market share Throughout the period, the bulk of ... importantly, in the course ofthe past few years, the ECB has put in the limelight three major traits that characterise how the international role ofthe euro has unfolded so far The first is that the euro’s...
... Identification of a mutation in the SF21 carB allele Replacement ofthe wild-type carB allele by carB36 The carotenoid pattern ofthe mutant SF21, i.e the accumulation of c-carotene and the subsequent deviation ... [33] The carotenoid pathway of N crassa coincides with that of F fujikuroi in the synthesis ofthe same end-product, the apocarotenoid neurosporaxanthin, but both fungi differ in the order ofthe ... sequence in the genome of a carS strain derived from T5 The recombination shown occurs at the opposite side fromthe one that produced the plasmid integration, leaving the mutated carB allele in the...
... mutation of surface residues located far fromthe heme moiety Thus, the thermodynamic driving force for the reaction of cyt c6 with PS I was essentially unaltered bythe introduction ofthe mutations ... inspection ofthe data revealed interesting deviations fromthe general trend A reversal ofthe charge effect ofthe mutation K8D in the mutant D72K ⁄ K8D did not lead to recovery of wild-type ... neutralization ofthe effect ofthe mutation D72K on the protein’s global charge was not sufficient to fully reverse the negative effect on the stability of cyt c6 The apparent stability ofthe mutants...
... died in the heart ofthe bravest, when at the close of day on the tenth of January, twenty-five days fromthe date of leaving Donner Lake, they saw an Indian village at the edge of a thicket they ... AND I TAKEN FROMTHE LAKE CABINS BYTHE THIRD RELIEF NO FOOD TO LEAVE CROSSING THE SNOW REMNANT OFTHE SECOND RELIEF OVERTAKEN OUT OFTHE SNOW INCIDENTS OFTHE JOURNEY JOHNSON'S RANCH THE SINCLAIR ... stretched the summer range ofthe antelope, deer, elk, and buffalo The effort to keep out ofthe way ofthe Pawnees, and the desire to catch sight ofthe big game, urged us on at a good rate of speed,...