... Regulation of the rat sarcoplasmic reti-culum calcium release by calcium. J Muscle Res CellMotil 21, 131–138.20 Lai FA & Meissner G (1990) Structure of the calciumrelease channel ofskeletalmuscle ... presence of 18 nm ryanodineand the amount of ryanodine bound was plottedagainst the concentration of the gadolinium (Fig. 7).Fitting of data points with the Hill equation revealed aKdvalue of ... CoronadoR (1988) Ryanodine receptor ofskeletalmuscle is a gapjunction-type channel. Science 242, 99–102.13 Sitsapesan R & Williams AJ (1995) The gating of thesheep skeletal sarcoplasmic reticulum...
... standardization of the loading. (D) Weights of injected TA muscles (n = 13) were compared to those of control samples. No significant difference wasobserved. (E) Histological analyses of muscles. Frozen ... obtain denervation of the muscleof the lower limb,the dorsal skin of the thigh of 2-month-old male129SvPasIco mice (n = 6 for each time point) was cut,and the posterior muscles were split ... waschecked at the time ofmuscle excision by visualization of abnormal gait of the limb and by verifying the disconti-nuity of the sciatic nerve at the thigh. The TA, EDL andsoleus muscles were sampled...
... domain of TnC [33]. The crystal structure of cTn [20] showed that residues R117 to L128 of TnI (inchicken fast skeletal numbers) are in close contact withthe N domain of TnC in the presence of ... terminus of TnI has a tubular-like conformation that involves theglobular N domain of TnC. NMR studies have provi-ded additional information on the structure of skeletal TnC(1–91) in the presence of ... structure of TnI and TnC that can explainhow they interact during muscle contraction. Figure 6shows a schematic representation of the structural dis-position of TnC and TnI in the light of recent...
... release of ADPdepolymerised actindenaturedactinrandom aggregationafter release of pressure[1][2][3]Fig. 12. Schematic interpretation of thebehaviour of F-actin in the presence of free ... 250 MPa.However, the increase of intensity of ¯uorescence of eATPbuffer itself was much smaller than that of eATP bo und toG-actin. Therefore, the increase of ¯uorescence seems to b edue ... thecentre of spectral mass of intrinsic ¯uorescence spectrum of G-actinwithATP(0.5mgámL)1, p H 7.5) in a pressurerange from 0.1 MPa to 400 MPa at a ®xed temperature of 20 °C. The transition of the...
... the mass of the testing end of myometer; amaxis the maximal amplitude of oscillation, and ∆l is thedepth of the displacement of the testing end.Measurements for determination ofmuscle tone ... characteri-zation of the skeletalmuscle may provide new insightsinto muscle function and can help to diagnose the stage of pathological processes of muscles.The testing end (mass 20 grams) of the computerizedmyotonometry ... during research (see subdivision of workers into 3 categories according to MYO data) areindicative of several features of HFF and LFF.Features of HFF:- Force of muscles is restored quickly after...
... viscosity of t he extract as a result of D NA, 2 lLofDNase I (200 units) was added per 100 lL of buffer [30].Following filtration through two layers of miracloth a nd theaddition of four volumes of ... usedwere of analytical grade and purchased from SigmaChemical Company.Preparation of total muscle extractsFor the comparative gel electrophoretic analysis of normalvs. dystrophic skeletalmuscle ... analysis of Fig. 3 and the 1D i mmunoblotting of Fig. 4.As the full-length Dp427 isoform of dystrophin does notenter the second dimension of conventional 2D gels, theexpression level of a-dystroglycan...
... 4. Immunoblot of CK isoforms of different post-operativehuman tissues. (A) Immunoblot of MCK (a) and sMitCK (b) of nor-mal muscle (N) and fibrosarcoma (T) tissue. (B) Immunoblot of BCK(a) and ... Expression of mRNA of MCK and sMitCK isoforms of crea-tine kinase and b-actin in normal muscle (NM) and 3MC inducedsarcoma tissue (ST) of mouse.1.00.80.60.40.2Relative expression of mRNA0.0NMCK ... that because of the lowabundance of MCK and sMitCK isoenzymes insarcoma, a much higher amount of protein had to beapplied compared with normal muscle. Detection of BCK and uMitCK isoforms in...
... partner ofskeletalmuscle titin-N2A [18]. An analysis of CARP expression in the subcellular compartmentsobtained from the muscleof the mice injected withpYFP-CARP-CFP-HIS confirms the presence of ... althoughthe over-expression of CARP in muscle cells results inNF-jB p65 inhibition, it does not affect the expression of MURF1, which is one of the main mediators of NF-jB-dependent muscle atrophy [51]. ... thecontrol of the NF-jB pathway because calpain 3deficiency is associated with an altered distribution of both NF-jB and of its regulator IjBa [8], as well aswith blockade of the induction of specific...
... of this paper was to investigate thepathophysiological roles of muscle- specific miRNAs inDM1, the most frequent autosomal dominant myopathyin adults. We therefore profiled the expression of ... pathohistological features of each DM1 specimens used in this study (sex, muscle, lysosomal activity and muscle pathohistological aspects). Muscle biopsies from VL of 1 female and 3 males have ... al., Overexpression of microRNA-206 in the skeletalmuscle from myotonic dystrophy type 1 patients Journal of Trans-lational Medicine 2010, 8:48Gambardella et al. Journal of Translational Medicine...
... support the postulated sensing activity of hSGLT3 [33] in skeletal muscle. However, the increased expression of hSGLT3 in skeletalmuscle we found after 16 weeks of resistance exercise without a ... predictor of hSGLT3 transcript levels, explaining 68% of its variability (P=0.01). Our data show that hSGLT3, but not GLTU4, expression was enhanced in skeletalmuscle after 16 weeks of resistance ... important factor. Exercise, the major physiological activator ofmuscle glucose transport, regulates the expression of GLUT4 in skeletal muscle [3, 4], and induces its translocation from the intracellular...
... into Pathologyof Ocular Zoonoses Hans E. Schaefer Institute ofPathology - University of Freiburg im Breisgau (Germany) Published: 2009.03.19 Zoonosis: what does it mean? The meaning of the ... consequence of cystic larval growth and inflammatory reaction due to eventual necrosis of parasites. - Analogous intraocular lesions may be caused by coenuriasis, the larval stage of tape worms of ... Organization produced an official definition of Zoonosis as: “diseases transmit-ted in a natural way from vertebrate animals to hu-mans”. Currently, three subtypes of Zoonoses may be distinguished:...
... TL,Catalona WJ. Long-term followup of patients treatedwith 1 or 2, 6-week courses of intravesical bacillusCalmette-Guerin: analysis of possible predictors of response free of tumor. J Urol 1990;144(3):652-7.Cordon-Cardo ... butmost recurrences of non -muscle- invasive bladdercancer (especially of initially diagnosed grade 1,stage Ta disease) are not muscle invasive and car-ry relatively little risk of metastasis or ... intravesical thiotepa in themanagement of non-invasive carcinoma of the blad-der. J Urol 1981;125(3):307-12. Koss LG. Tumors of the urinary bladder. In Atlas of tumor pathology, second series, fascicle...
... Fundamental review of the trading book instruments that form part of a revised trading book. The Committee intends to consider the timing and scope of further work on the capitalisation of interest ... Bank of New York Mr Karl Reitz Federal Deposit Insurance Corporation, Washington, DC Mr Roger Tufts Office of the Comptroller of the Currency, Washington, DC 24 Fundamental review of ... two broad sets of factors: (i) the characteristics of the market itself in times of stress; and (ii) the characteristics of banks’ portfolios relative to the market (eg size of exposures relative...
... may also occur in nonmuscle Tmisoforms due to replacement of the N-terminal muscle exon 1a by nonmuscle exon 1b.The noncooperative unfolding of a significant part of the nonmuscle Tm molecule ... to isoform type. In mam-malian cells, alternative splicing produces a variety of muscle and nonmuscle isoforms from four differ-ent genes [1]. Muscle cells express two major iso-forms of Tm ... to investigate thermal unfolding of recombinant fibroblast isoforms of a-tropomyosin (Tm) in comparison with that of smooth muscle Tm.These two nonmuscle Tm isoforms 5a and 5b differ internally...
... states of regulated actin are important determinants of the regu-lation of striated muscle contraction. The distribution of these states determines the ATPase activity, whereasthe rates of transitions ... troponin T,troponin I and troponin C, which bind along actin fila-ments ofskeletal and cardiac muscles. Activation of striated muscle contraction occurs when Ca2+binds totroponin C, or in a Ca2+-independent ... reliable meth-ods of determining the state of actin in real time. Thismanuscript explores, in detail, a well-known method of monitoring the state of regulated actin.The fraction of actin in the...