pathology changes of human nasal epithelial stem or progenitor cells hnespcs from in vitro and air liquid interface ali culture system in nasal polyp np
Study of human nasal epithelial stem or progenitor cell growth and differentiation in an in vitro system
... (hNESPCs) from in vitro and air- liquid interface (ALI) culture system in nasal polyp (NP) …………………………………………………………………………… 107 5.1 Pathology change of hNESPCs in vitro culture system in NP 107 5.1.1 Results ... to distinguish between the normal and abnormal mechanisms underlying epithelial repair and remodeling Therefore, culturing and comparing human nasal epithelial stem cells from healthy and diseased ... properties of epithelial stem or progenitor cells in a chemical-defined medium Meanwhile, these cells successfully differentiated into functional cells (e.g., ciliated and goblet cells) in air- liquid interface...
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Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc
... use for this purpose is limited Transcriptome in hepatic induction of AT-MSCs by donor shortage and the difficulties involved in adequate propagation and long-term maintenance of normal human ... ethics, cost and time Considerable emphasis has therefore been placed on the development of in vitro test systems Although primary human hepatocytes are the best source of cells for such systems, their ... Expression of liver-selective transcription factors, such as HNFs, CCAAT ⁄ enhancer-binding proteins and GATA-binding proteins, is essential for the induction of liver development and its progression...
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báo cáo hóa học:" BRCA1/2 genetic background-based therapeutic tailoring of human ovarian cancer: hope or reality?" ppt
... therapeutic strategies in this specific setting Clinical findings Preliminary clinical evidence appears in line with preclinical in vitro findings and indicates that prospective clinical trials must ... regulatory factor in the molecular events leading to the development of cancer or inflammatory disease In vitro and in vivo studies support the use of PARP inhibitors not only as chemo and radiosensitizing ... joining (NHEJ) and single-strand non-homologous end-joining annealing (SSA), which are error-prone DNA repair pathways Deficiency in DSB repair plays a crucial role in the chemo sensitivity profile...
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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps
... signaling; and WNT signaling There is a substantial body of work linking Wnt signaling with stemness and malignant behavior (reviewed in [36]) With respect to prostate cancer, Wnt signaling has ... present in the stem cell population TJP1 (ZO-1) and TJP2 (ZO-2) proteins were also expressed by the majority of progenitor and stem cells from tumor cell cultures (Figure 4c,d), whereas only a minority ... Distinctive stem cell and tumor signatures are found in human prostate cancers containing a minimum Gleason score pathology Distinctive stem cell and tumor signatures are found in human prostate...
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Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx
... mainly the initial folding and stability of the tetramer, and similarly lowered levels of tetramer in human cells would account for the observed elevation of indicator acyl-carnitine levels From ... to the purified protein (assuming no major change in the extinction coefficient of the bound cofactor in the case of the mutants) [15] The peak absorbance ratios showed first of all that the wild-type ... responsible for the inactivity of the R256T mutant protein Thermostability of the purified mutant proteins The effect of temperature on MCAD stability was directly investigated by incubating aliquots of...
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Báo cáo y học: " Inhibition of constitutively active Jak-Stat pathway suppresses cell growth of human T-cell leukemia virus type 1-infected T-cell lines and primary adult T-cell leukemia cells" potx
... tyrosine kinases, including Lck, Lyn, Btk, Syk and Src [16,17] Systemic administration of AG490 in SCID mice with disseminated human leukemic cells dependent on Jak2 for survival resulted in tumor ... phosphorylation of Jak1, Jak2 and Jak3 was observed in MT-2 and HUT-102 cells, and treatment of these cells with increasing concentrations of AG490 resulted in significant inhibition of phosphorylation ... HUT-102 cells In contrast, p53 protein was almost undetectable in ED40515(-) cells and remained unchanged in AG490-treated cells p21 was induced in MT-2 and HUT-102 cells and remained undetectable in...
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Báo cáo y học: "nalysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET" pptx
... four or more overlapping reads in OCT4, and three or more overlapping reads in the case of NANOG Gene exons and introns are represented by pink boxes and solid horizontal lines, respectively For ... bacterial cloning and sequencing steps, as well as mapping issues, introduce scope for error We feel that a combination of more stringent mapping criteria and the inherent noise in the sequencing procedure ... to culture these cells Finally, different binding results may be obtained due to differences in ES cell strains Therefore, with the availability of binding information from different cell strains...
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The impact of human resource management practices on employee's job satisfaction in Vector Aviation Co., Ltd
... train staffs Second type of training in Vector is oversea training 29 courses Those are organized by airlines where Vector acts its distributor If airline has any training program for distributors, ... collecting and recording job information, checking the job information for accuracy, writing job description based on the information, using the information to determine what skills, abilities, and ... teaching or training programs to meet the new jobs demand of aviation services In addition to that, air logistics industry is also facing the severe shortage of well-trained workforce Dealing with...
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ASSESSMENT OF THE BENEFITS OF EXTENDING THE TROPICAL RAINFALL MEASURING MISSION A PERSPECTIVE FROM THE RESEARCH AND OPERATIONS COMMUNITIES doc
... especially for monitoring and forecasting the tracks and intensity of tropical cyclones and the intensity of rainfall they yield Continuation of the TMI data stream would enable modelers and forecasters ... centers in Australia and New Zealand routinely incorporate TMI data in their tropical cyclone forecasting Indeed, at the 2002 International Workshop on Tropical Cyclones in Cairns, Australia, presenters ... timing for reaching controlled reentry fuel threshold of 138 kg and a fiscal year 2004 Program Operating Plan guideline aCost of maintaining staffing of flight control bCost of analysis, training,...
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Báo cáo lâm nghiệp: "Discrimination of coated carbide tools wear by the features extracted from parallel force and noise level" potx
... Pattern recognition of cutting sound from woodworking tools and its application to the in- process monitoring of wear, in: Proceedings of the 11th International Wood Machining Seminar, 1993, pp 147–156 ... down into the corner of the cutting edge in the speed of 0.05 mm/rev When the cutting tools finished one pass of cutting (cutting from bottom face to top face of the disk with cutting length of ... investigated For this discussion, relation between parallel force and cutting speed for the final cutting length is provided in Figure The increase in the parallel force with an increase in cutting speed...
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Báo cáo y học: " Early detection of Varicella-Zoster Virus (VZV)-specific T-cells before seroconversion in primary varicella infection: case report" potx
... as for example in cases of atypical or subclinical varicella or in the absence of detectable VZV DNA in plasma Consent Written informed consent was obtained from the patient for publication of ... knowledge, no systematic comparison between the onset kinetics of seroconversion and occurrence of VZV-specific T -cells within patients at early stages of varicella infection has been performed until ... report and any accompanying images A copy of the written consent is available for review by the Editor -in- Chief of this journal Additional file 1: Protocol for the detection of VZV-specific T -cells: ...
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Báo cáo y học: " Identification of super-infected Aedes triseriatus mosquitoes collected as eggs from the field and partial characterization of the infecting La Crosse viruses" pot
... transported to the insectaries at the Arthropod-borne and Infectious Diseases Laboratory (AIDL) at Colorado State University (CSU), Fort Collins, CO and maintained in the insectary until hatching and ... following program: 94°C for min, 37 cycles of [94°C for min., 57°C for and 72°C for 2.5 min] followed by a final extension at 72°C for Sequencing PCR products were separated by electrophoresis in ... infection or mating advantages resulting from such infection These two factors may help maintain stable LACV prevalence from year to year in the vector population Conclusions Processing of individual...
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Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps
... PBS-buffered formalin solution as well as deep-frozen in liquid nitrogen and stored at -70°C Immunofluorescence staining of tissue specimens The formalin-fixed tissue was paraffin-embedded and cut ... sites of traumatic, degenerative, or ischemic injury and thus promote repair or the formation of new vessels [13,45-52] Whereas in blood the mature endothelial cells may originate from sloughing off ... lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) BS-I (green) – Orange reveals staining for red and green) F(a): Staining of EPC in culture with DAPI (blue) and FITC-labeled lectin from...
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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... Isolation of lineage negative progenitor cells For the purified progenitor cell transfusion experiments, lineage negative progenitor cells (Lin- PCs) were separated from whole BMCs using a mouse progenitor ... frequency of QD-positive cells was measured The numbers of donor cells in circulation and BM were calculated as follows: Number of QD-labeled donor cells in circulation = Fraction of donor cells in ... patients These findings suggest that progenitor cells acquire the capability to remain in the circulation during systemic inflammation for subsequent sequestration into target organs upon demand The...
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Báo cáo y học: " A comparative study of a theoretical neural net model with MEG data from epileptic patients and normal individuals" docx
... rectangle) for positioning of the SQUID [14-16] The MEG was recorded from each temporal lobe at each of the 32 matrix points of the scalp for 32 s and was band-pass filtered with cut-off frequencies of ... (assuming that the ratio of excitatory and inhibitory synapses is the same in both situations so that the inhibitory inputs may be disregarded for the moment) Then it is clear that firing in neurons ... the number of elements in the ith interval of the normalized MEG histogram Ti: is the number of elements in the ith interval of the normal distribution with the same mean value and standard deviation...
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Báo cáo y học: " Effect of neutrophil elastase and its inhibitor EPI-hNE4 on transepithelial sodium transport across normal and cystic fibrosis human nasal epithelial cells" pot
... serine proteases such as epithelial CAPs Indeed, inhibition of endogenous CAPs with aprotinin induced a sustained decrease in Isc in both normal and CF HNEC, supporting this hypothesis Interestingly, ... Procedures Primary cultures of human nasal epithelial cells (HNEC) Nasal polyps (NP) were obtained from non CF (n = 9) or CF (ΔF508/ΔF508, n = 4) patients requiring surgery for their nasal polyposis as ... of penicillin, 100 mg/ml of streptomycin, 2.5 μg/ml of amphotericin B and 100 mg/ml of gentamicin) and transported to the laboratory for cell isolation Briefly, NP samples were rinsed in phosphate-buffered...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... salvage of hESC culture during infrequent occurrences of incubator break-down and power failure, either in small-scale culture for research or large-scale culture within bioreactors Conflicts of interest ... sides with parafilm (for minimal evaporation), and placed in either a 4oC refrigerator, or back into the incubator reset at 25oC An atmosphere of 5% CO2 was maintained only for the physiological ... cannot be said of human embryos, which are much less robust and hardy compared to mouse embryos Hence laboratories involved in handling human embryos in clinical assisted reproduction often have...
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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
... an internal standard for determination of the void volume, V0, in the individual experiments Prior to injection, hTK1 was diluted to lgÆmL)1 and incubated with mM of the indicated nucleotides or ... structural conditions for ATP binding to human TK1 In the first crystal structure of TK1-type enzymes of human and mycoplasmic origin [14], the feedback inhibitor dTTP was bound in the substrate pocket, ... low or high catalytic efficiency Therefore, the various incubated hTK1 forms from Figs and were analyzed for their kinetic behavior with dThd, and the results are presented in Figs and Only in...
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Tài liệu Báo cáo khoa học: Transcriptional upregulation of inflammatory cytokines in human intestinal epithelial cells following Vibrio cholerae infection pptx
... of cytokines upon V cholerae infection in intestinal epithelial cells Reports have shown the induction of IL-8 in intestinal epithelial cells upon V cholerae infection [11–13] The induction of ... upregulation of many proinflammatory mediators, such as cytokines [14] However, little is known about the role of V cholerae in initiating and sustaining the innate in ammatory response in intestinal epithelial ... does indeed induce a range of cytokine expression in intestinal epithelial cells upon infection Comparison of the status of induction of cytokines by quantitative realtime RT-PCR in Int407 and...
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