parameters scattering simulation models and commercial thin film solar cell simulator asa
Effects of anti malaria drug artesunate in the treatment of rhinovirus induced asthma exacerbation
... Th2 cells (IL-4, 9, 13, and 25), Th3 or T regulatory cytokines (IL-10 and TGF-β) and TH17 cells (IL-17A and 17F) Other cytokines include proinflammatory cytokines (IL-1β, 6, and 11, TNF-α, and ... inflammatory and immune response are regulated by a number of cytokines and chemokines, which are secreted from structural and inflammatory cells, such as the eosinophils, bronchial epithelial cells and ... allergens in asthma, which include T cells, eosinophils, mast cells and neutrophils (FIG.1-2) The airway epithelial cells are located at the interface between the host and the environment; therefore,...
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Báo cáo y học: "Differential clinical efficacy of anti-CD4 monoclonal antibodies in rat adjuvant arthritis is paralleled by differential influence on κ α NF-κB binding activity and TNF-α secretion of T cell" pot
... differential contribution of the CD4+ and CD8+ T -cell subpopulation and suggests that interactions between CD4+ and CD8+ T cells [S9] are critical for T -cell activation and the effects of anti-CD4 mAbs ... were kept under standard conditions, two per cage, with food and water ad libitum and a 12 h/12 h light/dark cycle The mouse anti-rat-CD4 mAbs W3/25, OX35, and RIB5/2 (IgG1, IgG2a, and IgG2a, respectively) ... Lab, 20 àg/1 ì 107 cells) for hour at 37C After washing, the cells (1 ì 106 per ml and per well) were seeded in 24-well plates previously coated with R73 and harvested after 24 and 48 hours Cytokine...
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Báo cáo khoa học: Akt-dependent phosphorylation negatively regulates the transcriptional activity of dHAND by inhibiting the DNA binding activity pdf
... indicate the positions of dHAND mutants (B) Coimmunoprecipitation assay of dHAND and Akt AU5-dHAND was immunoprecipitated from the cell lysate of 293T cells expressing AU5-dHAND and HA-Akt These samples ... GST-dHAND-(1–102) efficiently, indicating that dHAND may bind to Akt via its bHLH domain To detect the interaction between dHAND and Akt in vivo, we coexpressed AU5-dHAND and HA-Akt in 293T cells and ... Effects of Akt overexpression on dHAND phosphorylation Cell lysates were prepared from 293T cells expressing AU5-dHAND and HA-Akt, and the phosphorylation status of dHAND was analyzed by immunoblotting...
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Báo cáo khoa học: Photosynthetic acclimation: Structural reorganisation of light harvesting antenna – role of redox-dependent phosphorylation of major and minor chlorophyll a/b binding proteins pot
... miniseries) The precise mechanisms and molecular components of state transitions appear to differ between the aquatic unicellular green alga Chlamydomonas reinhardtii and land plants In particular, the ... wild-type (D, E) and CP29-less (F) C reinhardtii cells placed in State (D) and State (E, F) Modelling is based on higher plant coordinates 1QZV.pdb for the higher plant LHCI–PSI [54] and 1RWT.pdb ... PsaG (purple), PsaI (orange), PsaL (cyan) and PsaH (white, arrowed in d and f) Chlorophylls are shown in yellow and for clarity were excluded from LHCI and CP29 subunits The additional density...
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Báo cáo khoa học: The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97 doc
... (residues 14–194) and yeast Ufd1 (residues 1–210) were constructed by a standard PCR protocol, and subcloned into pGEX-4T3 (Amersham Bioscience) The cells were grown to a D600 of 0.5, and heterologous ... with CLUSTALX [60] and manually edited The secondary structure elements are shown at the top, with thick line segments for the a-helices (a1–a4) and thin line segments for b-strands (b1–b14) The ... hydrophobic residues and basic residues of PEX1-ND are indicated by closed circles and closed squares, respectively (see text) The conserved residues and class-specific residues of PEX1-ND and VCPND are...
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Báo cáo khoa học: CpG methylation of the CENP-B box reduces human CENP-B binding pptx
... (lanes and 6), 0.5 lM (lanes and 7), lM (lanes and 8), lM (lanes and 9), and lM (lanes and 10) (C) Graphic representation of the complex formation rates shown in panel B Unmethylated (d) and methylated ... CENP-B(1–129) and the CENP-B box DNA (A) Schematic presentation of the complexreconstitution assay CENP-B(1–129) and the CENP-B box DNA strands are shown in blue and red, respectively CENP-B(1–129) and ... purified CENP-B(1–129) protein and the CENP-B box DNA strands (1 lm for each strand) were mixed in the presence of m urea, 500 mm NaCl, mm dithiothreitol and 0.1 mgÆmL)1 BSA, and the volume of the reaction...
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Báo cáo khoa học: A sucrose binding protein homologue from soybean exhibits GTP-binding activity that functions independently of sucrose transport activity pptx
... leaves, the companion cells of mature phloem and the cells of cotyledons undergoing differentiation [9,10] In the cotyledon, expression of the SBP gene is temporally regulated and accumulation of ... elements in fully expanded leaves, shoots and roots [11,12] and in V faba developing seeds, SBP was colocalized with the H+/sucrose symporter in the plasma membrane of transfer cells [13] A SBP homologue ... soybean cells Total protein from electroporated cells (1) and control cells (2) was extracted days postelectroporation, fractionated by SDS/PAGE and immunoblotted with an anti-S64 serum Lane is...
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Báo cáo khoa học: Dynamics, stability and iron-binding activity of frataxin clinical mutants pdf
... [14] Residues in the loop between strands b4 and b5 (Thr149, Asn151 and Lys152), and at the end of strand b6 (Val174), have larger than average T1 ⁄ T2 ratios and shorter T2 (see supplementary Figs ... gel bands (n = 3) restricted to particular regions of the protein: fast degradation is observed at cleavage sites within helix a1 (R97), at strand b5 (R165) and on the loop between strands b5 and ... were drawn using the protein databank file 1EKG turn formed by G128, S129 and G130 between strands b1 and b2 and both / and w are positive Its mutation into a valine must disturb the turn conformation,...
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Báo cáo khoa học: Replacement of helix 1¢ enhances the lipid binding activity of apoE3 N-terminal domain pot
... reducing (lanes and 2) or nonreducing (lanes and 4) conditions and was stained with Coomassie Blue Lanes and 3, WT apoE3-NT; lanes and 4, HT apoE3-NT the presence of monomers and dimers was obtained ... surfaces [6] In this scheme the helix bundle opens via helix and moving away from helix and 3, with the segments connecting helix and and helix and serving as ‘hinges’ (Fig 9B) It has been suggested ... and different amounts of receptor binding competitor for h at °C The medium was removed, and the cells were washed five times with chilled NaCl ⁄ Pi-FAFA and two times with chilled NaCl ⁄ Pi Cells...
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Báo cáo y học: "The utility of pathway selective estrogen receptor ligands that inhibit nuclear factor-κB transcriptional activity in models of rheumatoid arthrits" pps
... with standard operating procedures and were provided with food and water ad libitum All experiments were approved and performed in accordance with the Wyeth Animal Care and Use Committee standards ... (ERα and ERβ) Both receptors are widely distributed throughout numerous organs [18] and are present in T cells, monocytes, dendritic cells, synovial macrophages, articular chondrocytes, and proliferating ... Applications, Inc The cells were cultured in synoviocyte growth medium (Cell Applications, Inc.) and seeded at × 105 cells per well in a 12-well dish After overnight culture, the cells were pretreated...
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Báo cáo y học: " Inhibitor of IκB kinase activity, BAY 11-7082, interferes with interferon regulatory factor 7 nuclear translocation and type I interferon production by plasmacytoid dendritic cells" ppsx
... 96-well plates at × 104 cells (2 × 105 cells for PBMCs) in the final 200 ml of medium per well for 24 h Lupus PBMCs and serum, and preparation of necrotic cell supernatants PBMCs and sera were obtained ... and wrote the paper TI planned, designed and wrote the paper SN, RA, TK, and SF contributed to the experimental planning and design HA, YK, MO, NM, and KS performed the experiments of human cells ... stimulation, cells were fixed and permeabilized using the FIX and PERM kit (Invitrogen, Carlsbad, CA, USA) and then stained with FITC-labeled anti-IFN-α2 mAb (Chromaprobe, Maryland Heights, MO,...
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r.b.''s activity book 1
... puzzles, activities and brain-teasers They’re fun to – and they have important information for you to discover and share with your family, too Meet R.B He’s learning all about the things he can to ... eating and activity good for them – but it helps them the everyday things they love to and them better! I Spy Kitchen Safety at Home! Keeping food safe at home is easy! See how many safe and ... piece and places it in the space marked START The youngest child gets to flip a coin to begin the game If the coin lands ‘heads’, move spaces; if it lands ‘tails’, move spaces If the player lands...
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r.b.''''s activity book 2
... puzzles, activities and brain-teasers They’re fun to – and they have important information for you to discover and share with your family, too Meet R.B He’s learning all about the things he can to ... right, and again before crossing T Never run into the between cars 11 Make sure to obey traffic signals C and guards G Wear reflective clothing at and ... Find the words that show good and bad things about bullies and how to avoid them Use the pictures to get more “anti-bully” ideas Words are down, across, or horizontal and are all spelled forward...
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r.b.''s activity book 4
... puzzles, activities and brain-teasers They’re fun to – and they have important information for you to discover and share with your family, too Meet R.B He’s learning all about the things he can to ... fruits, vegetables and yogurt! R.B says, “Be Sure To Brush ” • Top • Bottom • Inside • Outside And don’t forget your tongue! Have FUN! “Share Your Smile” Seek -and- Find Circle the 13 things hidden ... to keep your smile healthy and bright • Wash your hands in the right way and at the right times • Make safe choices every day has learned all about staying healthy and safe (insert name) This...
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Báo cáo Y học: Barley a-amylase Met53 situated at the high-affinity subsite )2 belongs to a substrate binding motif in the bfia loop 2 of the catalytic (b/a)8-barrel and is critical for activity and substrate specificity pot
... acids (D179AMY2, E204AMY2, and D289AMY2 and D206TAA, E230TAA, and D297TAA) The invariant Y51AMY2 and Y82TAA are at subsite )1 as are H92AMY2 and H122TAA; M52AMY2 (M53AMY1) and W83TAA are at subsite ... highest afnity of the 10 subsites in AMY1 and AMY2 [44] Subsites )6 and +1 were almost as strong, while )5, )4, +2, and +4 contributed intermediate, and )3 and + very weak binding energy The catalytic ... reminiscent of the contact between ligand and the equivalent Trp and Gln in other a-amylase structures [7,8,14,1725,27] It is noticeable that subsite )2 of AMY1 and AMY2 had the highest binding afnity...
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Tài liệu Báo cáo khoa học: Secondary substrate binding strongly affects activity and binding affinity of Bacillus subtilis and Aspergillus niger GH11 xylanases docx
... Non-hydrolytic disruption of crystalline structure of cellulose by cellulose binding domain and linker sequence of cellobiohydrolase I from Penicillium janthinellum Acta Biochim Biophys Sin 33, 13–18 ... both XBS and XAN involved in substrate interaction were selected based on the crystal structures of XBS and XAN soaked with xylotetraose and xylopentaose, respectively [12] (Fig 1), and were ... = 9.76 · 10)10 M and AzU = 50.8 · 10)10 M and for the wild-type XAN, X6U = 1.02 · 10)10 M, XyU = 7.72 · 10)10 M and AzU = 9.65 · 10)10 M for the activity on X6, Xylazyme AX and Azo-wheat AX,...
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Tài liệu Báo cáo khoa học: The chitinolytic system of Lactococcus lactis ssp. lactis comprises a nonprocessive chitinase and a chitin-binding protein that promotes the degradation of a- and b-chitin doc
... concentrations Sequence analysis and modelling of LlChi18A and LlCBP33A Cloning and purication of LlChi18A and LlCBP33A The gene fragments coding for the mature proteins of LlChi18A and LlCBP33A were successfully ... activity on cellulosic materials Eur J Biochem 269, 42024211 Cosgrove DJ & Tanada T (2007) Use of gr2 proteins to modify cellulosic materials and to enhance enzymatic and chemical modication of cellulose ... either dedicated to cellulose degradation [43,44] or that can be exploited for this purpose (expansins [45]; see also [46]) exist Materials and methods Bacterial strains and plasmids and cultivation...
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Tài liệu Báo cáo khoa học: Regulation of cathepsin B activity by 2A2 monoclonal antibody docx
... [16] or directly degrade the extracellular matrix (ECM) proteins However, ECM degradation depends on the activity of both extracellular and intracellular proteases, and cathepsin B plays an active ... intracellularly and pericellularly (white arrow) Addition of the 2A2 mAb to the assay medium reduced the degradation of DQ-collagen IV (B) Degradation products are visible intracellularly and ... programmed cell death [12,13] Increased levels of cathepsin B protein and activity are found in tumour tissues and have been suggested as prognostic markers in patients with breast, lung, colon and...
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Tài liệu Báo cáo khoa học: Calcium-binding to lens bB2- and bA3-crystallins suggests that all b-crystallins are calcium-binding proteins pptx
... proteins and induce luminescence peaks at 492 nm and 547 nm via energy transfer from Trp and Tyr residues [32] Terbium binds to bB2- and bA3-crystallins and induces luminescence peaks at 492 and 547 ... calorimetry, analytical gel filtration and dynamic light scattering of the apo and holo forms of bA3- and bB2-crystallins There was no significant change in the stability and hydrodynamic radius of the ... b-crystallins are calcium-binding proteins M K Jobby and Y Sharma Table Binding constants and the enthalpy change of calciumand terbium-binding to bB2- and bA3-crystallins K, dissociation constant (M);...
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Tài liệu Báo cáo khoa học: Dual P2Y12 receptor signaling in thrombin-stimulated platelets – involvement of phosphoinositide 3-kinase b but not c isoform in Ca2+ mobilization and procoagulant activity pdf
... buffers and (ant)agonists Measurement of cytosolic cAMP and InsP3 Intracellular levels of cAMP and InsP3 in resting and activated platelets were measured as described previously [27] Basal concentrations ... relies on both phospholipase C and PI3-K activity PI3-Kb and not PI3-Kc mediates the P2Y12 effect on thrombin-evoked Ca2+ responses In man and mouse, the PI3-Kb (p110b) and PI3-Kc (p110c) isoforms ... al tirofiban from Merck, Sharp and Dohme (Haarlem, the Netherlands); Akt inhibitor and LY294002 from Calbiochem (La Jolla, CA, USA) ET-18-OCH3, manoalide, U73122 and U73343 came from Biomol (Plymouth...
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