... expression of flaA, flaB, lafA1 and lafA2 106 Fig IV.7 Comparative extracellular proteome analysis of AH-1S, ΔserA, ΔmepA and ΔserAΔmepA mutants 107 Fig IV.8 Comparative extracellular proteome analysis ... include the genus Aeromonas in the new family Aeromonodaceae based on 16S rRNA cataloguing, 5S rRNA sequencing and RNA-DNA hybridization data (MacDonell and Colwell, 1985; MacDonell et al., 1986) Two ... 1979) I.2.2 A hydrophila infections in humans Five Aeromonas species have been established as human pathogens: A hydrophila, A caviae, A veronii, A jandaei and A schubertii (Janda and Abbott, 1998)...
... hindrance, whereas the canonical loop conformation (BMP-2-like conformation) could form similar noncovalent interactions with the type I receptor (Fig 2) Although the noncanonical loop conformation ... association rates (kon · 104ÆM)1Æs)1) of the BMP-2–BMPR-IA and BMPR-IB : interaction In contrast, association, and especially dissociation, for interaction of BMPs with ActR-I and ActR-II are ... prechordal mesoderm Cell 90, 257–269 Tomizawa K, Matsui H, Kondo E, Miyamoto K, Tokuda M, Itano T, Nagahata S, Akagi T & Hatase O (1995) Developmental alteration and neuron-specific expression of bone...
... biochemical characterizations of the L9.2 mutants were performed, including hydrolysis at different mono- and divalent cation concentrations The corresponding 5¢-truncation constructs were analyzed at ... concentrations (5, 10 and 50 mM) and mM KCl A surprising observation was that the presence of 200 mM K+ (standard conditions) during the reaction has a negative effect on 3¢-SS hydrolysis Cleavage ... 3289–3302 25 Ikawa, Y., Naito, D., Aono, N., Shiraishi, H & Inoue, T (1999) Trans-activation of the Tetrahymena group I intron ribozyme via a non-native RNA–RNA interaction Nucleic Acids Res 27,...
... and 5’-GGA TCC TAG AAG GAG CTG GA-3’ for CCL7; 5’-CAG TCC ATG AGA AGG AGT CCA-3’ and 5’-AGA TCC TGC ACA GGA CTG TG-3’ for CCL8; 5’-AGG GCA TGG GTT TTA TTA TAT ATA TAT-3’ and 5’-TTT AAA AAT AAC ... GCA TGT GTA TAA AG-3’ and 5’-ATA GGT ATC CTG AAT AAA TGA GAA C-3’ for CXCL7; 5’-CAT GCT GGT GAG CCA AGC AGT TTG AA-3’ and 5’-CAC TTC TGT GGG GTG TTG GGG ACA AG-3’ for CXCL9; 5’- CGA TGC CTA AAT ... Ramana Davuluri for statistical data analyses This work is supported by National Institute of Health grants CA74294, and CA10815, by a grant from Corixa Corporation, by Intramural National Cancer...
... and 5’-GGA TCC TAG AAG GAG CTG GA-3’ for CCL7; 5’-CAG TCC ATG AGA AGG AGT CCA-3’ and 5’-AGA TCC TGC ACA GGA CTG TG-3’ for CCL8; 5’-AGG GCA TGG GTT TTA TTA TAT ATA TAT-3’ and 5’-TTT AAA AAT AAC ... GCA TGT GTA TAA AG-3’ and 5’-ATA GGT ATC CTG AAT AAA TGA GAA C-3’ for CXCL7; 5’-CAT GCT GGT GAG CCA AGC AGT TTG AA-3’ and 5’-CAC TTC TGT GGG GTG TTG GGG ACA AG-3’ for CXCL9; 5’- CGA TGC CTA AAT ... Ramana Davuluri for statistical data analyses This work is supported by National Institute of Health grants CA74294, and CA10815, by a grant from Corixa Corporation, by Intramural National Cancer...
... untreated animals at the same stage of infection were used as controls (chronic untreated) Among the HAART treated animals, one (#20929) had undetectable PVL at the initiation of therapy (day 0), ... viral load as well as SIV-RNA, total SIV-DNA and 2LTR SIV-DNA were compared in placebo and HAART-treated macaques using a nonparametric Mann-Whitney test Differences in 3TC and IDV concentration ... tissues were assessed by the same test The correlation between 3TC concentration and residual viral replication/load in tissue, were evaluated using a nonparametric Spearman correlation test Acknowledgements...
... suggests that some extrachromosomal plasmid DNA was maintained for at least months Southern blot analysis was done with liver and muscle (data not shown) samples at 30 days after immunization due ... tissue distribution of naked plasmid DNA-Hsp65 vaccine and RNA expression by months following intramuscular administration in mice Additionally, we investigated whether the plasmid DNA replicates ... DNA vaccination Nature 1999, 400:269-271 Bonato VL, Lima VM, Tascon RE, et al.: Identification and characterization of protective T cells in HSP65 DNA-vaccinated and Mycobacterium tuberculosis-infected...
... notion that uPA secretionisa characteristic feature of activated macrophages [25] Additionally, secretion of proteoglycans in monocytes and macrophages increases when the cells are activated ... the action of uPA, can cleave matrix proteins such as fibronectin, laminin and aggrecan, and also activate matrix- and membrane associated MMPs, fibroblast growth factor and transforming growth factor ... extracellular uPA was caused by post-translational mechanisms However, we not know at which level uPA isdependenton proteoglycans One possibility is that uPA isdependenton proteoglycans after...
... pedestal formation Cell Microbiol 3, 745–751 Vlademir VC, Takahashi A, Yanagihara I, Akeda Y, Imura K, Kodama T, Kono G, Sato Y, Iida T & Honda T (2002) Cortactin is necessary for F-actin accumulation ... intermediate structure In an attempt to understand how pH and urea concentration affect the conformations of EspA, we constructed an EspA pseudo phase diagram with urea concentration as a function ... pH -Dependent EspA conformational change molecules, which assemble to form flagella filaments [20] The EspA-containing filamentous apparatus may form a conduit for translocation of bacterial proteins...
... 700 amino acid position B C1 a d a d C2 linker a d a d a d a d a d a MARV NP V Y QL AA KL RR LA L RE L AA I IV 315GVNVGEQYQQLREAAHDAEVKLQRRHEHQEIQAIAEDDEERKILEQFHLQKTEITHSQTLAVLSQKREKLARLAAEIENNIVEDQG400 ... Coomassie blue staining (to visualize the bacterially expressed proteins) and quantification of radioactive signals using BioImage analyzer BAS-1000 and the software TINA version 2.0, and Basreader ... of the plasmids was confirmed by automated DNA sequencing Detailed descriptions of cloning strategies are available on request Glutathione S tranferase (GST) pull-down assay pGex-NP and pGex-VP35...
... 700 amino acid position B C1 a d a d C2 linker a d a d a d a d a d a MARV NP V Y QL AA KL RR LA L RE L AA I IV 315GVNVGEQYQQLREAAHDAEVKLQRRHEHQEIQAIAEDDEERKILEQFHLQKTEITHSQTLAVLSQKREKLARLAAEIENNIVEDQG400 ... Coomassie blue staining (to visualize the bacterially expressed proteins) and quantification of radioactive signals using BioImage analyzer BAS-1000 and the software TINA version 2.0, and Basreader ... of the plasmids was confirmed by automated DNA sequencing Detailed descriptions of cloning strategies are available on request Glutathione S tranferase (GST) pull-down assay pGex-NP and pGex-VP35...
... values of r for any cell line, particularly for larger single fractions If this isa characteristic of all radioresistant glioblastoma lines, it not only suggests a rationale for large-fraction ... this variation based on genotype and fraction-size has a range of approximately 14, a remarkable difference for protocols that deliver the same total dose The schema at the 1) We have established ... xenografted human gliomas after fractionated radiotherapy Strahlenther Onkol 1989, 165(7):516-7 10 Rofstad EK: Fractionation sensitivity (alpha/beta ratio) of human melanoma xenografts Radiother Oncol...
... Kamelija, Turato Graziella, Ligabue Guido, Ciaccia Adalberto, Saetta Marina, Papi Alberto: Differences in airway inflammation in patients with fixed airflow obstruction due to asthma or chronic ... Johnson Malcolm, Vignola Anotonio M.: Asthma: from bronchoconstriction to airways inflammation and remodeling American Journal of Respiratory and Critical Care Medicine 2000, 161:1720-1745 Trautmann ... function techniques that are capable of assessing airway and parenchymal mechanics separately Total serum IgE was significantly elevated following systemic sensitisation but was not increased by aerosol...
... assembly of A3 G multimers, A3 G-Gag interactions, and virion incorporation of A3 G Conclusion We have developed a robust BiFC assay to analyze intracellular A3 G -A3 G, A3 G-Gag, and A3 G-RNA interactions ... RNA, head-to-head and headto-tail dimers may also form A3 G multimerization isdependenton RNA binding Our results showed that A3 G multimerization was dependenton an intact CD1 while mutations ... polyclonal antibody (Sigma), or a rabbit anti-c-Myc monoclonal antibody (Sigma) at a 1:5,000 dilution, followed by a HRP-labeled goat anti-rabbit secondary antibody (Sigma) at a 1:10,000 dilution...
... cross-react with A1 , A2 B, or A3 AR subtypes A2 B AR antibody is an affinity-purified rabbit polyclonal antibody raised against a region that corresponds to the second extracellular domain of A2 B AR ... Wellesley, MA, USA) To evaluate A2 B AR mediated cAMP accumulation, the reaction was carried out in the presence of selective A2 A antagonist SCH58261 at a concentration (100 nmol/l) able to block A2 A receptors ... reduction (by more than 50%) in NECA potency was observed, without any effect on agonist efficacy hypoxia, ischaemia and inflammation can stimulate adenosine production, A2 AR regulation has been...
... m) aa amino acid A/ E Attaching and effacing ATP adenosine triphosphate AU arbitrary unit AUC analytical ultracentrifugation BLAST Basic Local Alignment Search Tool BME β mercaptoethanol CCD charge ... (V.parahaemolyticus and V cholerae) YscN (ATPase), LcrH1 and-2 and SycE (chaperones), LcrE (structural “lid”) Environmental isolate, humans Aquatic isolate, humans Human pathogen Vibrio parahaemolyticus, ... 1993), Salmonella (Pang et al., 1995), E coli (Donnenberg and Kapper, 1992), Pseudomonas and various plant pathogens like Erwinia, Pseudomonas, Ralstonia, and Xanthomonas species (Bonas, 1994) A brief...
... cell adhesion, migration and intracellular signalling It was shown that purified CagL alone can directly trigger intracellular signalling pathways upon contact with mammalian cells and can even complement ... elongation by activating the Rap1 fi B-Raf fi Erk signalling cascade and by direct dephosphorylation and inactivation of focal adhesion kinase, FAK [7] Further studies have indicated that the CagAPY-induced ... phosphorylate injected CagA CagA can then modulate various signalling cascades associated with cell polarity, cell proliferation, actin-cytoskeletal rearrangements, cell elongation, disruption of...
... T, Shibagaki Y, Danbara H & Okada N (2010) Functional characterization of SsaE, a novel chaperone protein of the type III secretionsystem encoded by Salmonella pathogenicity island J Bacteriol ... Structural and biochemical characterization of SrcA, a multi-cargo type III secretion chaperone in Salmonella required for pathogenic association with a host PloS Pathog 6, e1000751 42 Miki T, Shibagaki ... Shigella and Salmonella uptake by macrophages in the early steps of invasion, and have the ability to induce membrane extensions (filopodia and lamellipodia) on macrophages [55,63] Specifically, IpaC...
... gene-pathogenicity island (cagPAI), a 40 kbp DNA fragment that is transmitted horizontally; see the accompanying review by Tegtmeyer et al [14 ] CagA is considered as a paradigm for bacterial carcinogenesis ... HP0525 ATPase activity and CagA transfer, a hypothesis supported by ATPase assays and in vivo observation of complex formation It is therefore likely that HP1451 acts as a negative regulator for ... action, epithelial cells will have some of their major functions disturbed, such as cell–cell adhesion, signalling, adherence and proliferation [17] CagA translocation and 1214 phosphorylation...