on the quot primary sequence quot of peptides and proteins
hplc of peptides and proteins, methods and protocols
... obtain the desired separation The optimization of high-resolution separations of peptides and proteins involves the separation of sample components through manipulation of both retention times and ... [X]s and [X]m refer to the concentrations of the solute in the stationary and mobile phases, respectively, and Vs and Vm are the corresponding volumes of the stationary and mobile phases Since the ... bind and exchange positively charged ions (cations) (see Fig 1B) In solution, proteins and other biomolecules are ionized, and the extent of ionization is dependent on the pH of the solution For...
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Tài liệu Báo cáo Y học: The RuvABC resolvasome Quantitative analysis of RuvA and RuvC assembly on junction DNA doc
... is the maximum response of RuvA binding, Rjunc is the response of the binding of the biotinylated Holliday junction, Mr RuvA is the molecular mass of RuvA and Mr junc is the molecular mass of the ... face of a Holliday junction, the effect of the addition of RuvC to the RuvA–Holliday junction complex was investigated Analysis of the interaction of RuvA and RuvC with the Holliday junction was ... that at low concentrations of RuvA (0.0026 and 0.026 nM) only very small amounts of protein bind to the Holliday junction even after 2.5 h of incubation Only after the concentration of RuvA is...
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Tài liệu Báo cáo Y học: Mutations in the docking site for cytochrome c on the Paracoccus heme aa3 oxidase Electron entry and kinetic phases of the reaction pptx
... the contribution of additional charge(s) located on the two further subunits of the native oxidase In focussing on the main interaction domain on subunit II, we introduced additional mutations ... shielded by increasing the ionic strength of the medium Under turnover conditions, an optimal salt concentration results from a compromise of the association and the dissociation rates for cytochrome ... for each donor concentration, and the average was fitted to the sum of two exponentials The observed rate constant from the fast phase of this double-exponential decay was plotted against the cytochrome...
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Báo cáo khoa học: Retrocyclin RC-101 overcomes cationic mutations on the heptad repeat 2 region of HIV-1 gp41 ppt
... that binds HR1 upon 6HB formation The strong affinity of RC-101 for HR2 prevents the interaction of HR1 and HR2, formation of the 6HB, and subsequent fusion of the host and viral membranes (Fig 4C), ... complex in the same region Examination of a helical representation of HR2 (Fig 4B) shows that the backbone of RC-101 covers the ‘a’ and ‘d’ heptamers, and the long flexible side chains of RC-101 ... found in gp120 and one each in the HR1 and HR2 regions of gp41, and all three mutations converted a polar or anionic residue to a cationic residue [14] In addition, the cationic mutation in HR2 ablated...
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Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
... respect to the role of HSP70 in the inhibition of the release of Smac and apoptosis GAPDH Ratio of HSP70 to GAPDH 14 # 12 # 10 * The role of the ATP-binding domain of HSP70 in the prevention of the ... domain of HSP70 is essential for the inhibition of H2O2-induced activation of caspases-9 and -3 and apoptosis (A) The effects of HSP70 and its deletion mutant proteins on the activation of caspases-9 ... cytotoxicity The protective effects of HSP70 were correlated with the level of HSP70 expression because the clone with higher HSP70 expression demonstrated a more significant reduction of the apoptotic...
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Báo cáo khoa học: Separation and characterization of caveolae subclasses in the plasma membrane of primary adipocytes; segregation of specific proteins and functions docx
... inhibition of SR-BI [17] Discussion Herein we have identified three caveolin-containing membrane fractions by their composition and cell surface accessibility, thus demonstrating the segregation of proteins ... membranes None of the three caveolae classes, which each represent about one-third of the caveolin, can for quantitative reasons therefore be explained as contamination by other membrane fractions containing ... involvement of SR-B1 in this uptake was indicated by the inhibition of the uptake by the SR-BI inhibitor BLT-1 Conclusions In conclusion, we demonstrate three subclasses of caveolae with compositions...
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Báo cáo khoa học: The ‘pair of sugar tongs’ site on the non-catalytic domain C of barley a-amylase participates in substrate binding and activity potx
... is the rst demonstration of a function for a C domain from the large GH clan-H The work contributes to the unraveling of the molecular basis of multivalent enzymepolysaccharide interactions One ... with the 5062 reduced DMA of the AMY1 Y380A sugar tongs mutant, these effects may stem from allosteric regulation The mutational analysis of the sugar tongs in barley AMY1 explored the role of ... to the active site cleft and the sugar tongs [21] The three rings dened at the Trp278Trp279 site corresponded to acarbose with the reducing-end glucose cleaved off and with the same orientation,...
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Báo cáo khoa học: Salicylic acid and the hypersensitive response initiate distinct signal transduction pathways in tobacco that converge on the as-1-like element of the PR-1a promoter pot
... PR-1a promoter, insertion of the as-1m4 mutation drastically reduced reporter gene expression from the strong )1533 bp promoter region (Fig 2B) On the other hand, induction of GUS activity by SA ... included necrotic tissue from the lesions, whereas leaf material of zone was collected in a region 7–14 mm away from the center of the lesions Due to the expansion of local lesions in nahG expressing ... mm Proteins were extracted from the samples and analyzed for the expression of PR-1 proteins Significant amounts of PR-1 proteins were detected in the samples from the narrow zone around lesions...
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THE EXPOSURE OF YOUTH TO UNWANTED SEXUAL MATERIAL ON THE INTERNET: A National Survey of Risk, Impact, and Prevention pdf
... supportive of different contentions about harm, depending on the emphasis one chooses to give them On one hand, it is clear from their reactions that the majority of youth regard their personal unwanted ... an average of 24% of the health information and 91% of the pornography Although such studies show possible weaknesses in the software approach, they not address the question of how the programs ... harmful On the other hand, there are findings from the study that bolster concern about harm: the ones showing that one quarter of those exposed said they were very or extremely upset and one fifth...
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báo cáo hóa học: " Effects of the physiological parameters on the signal-to-noise ratio of single myoelectric channel" doc
... a contraction [18-20] Some modelling work on motoneuron firing patterns suggested that the range of the firing rate of the motoneuron during a steady contraction is to 50 pps [21] On the other ... as the nonlinear processor, and the channel output is the convolution of the motor unit action potential (MUAP), m(t), with an innervation process u(t), which is the output of the motoneuron ... temporal and spatial summation of motor unit action potentials The results of their study showed that the SNR nonlinearly increased with the increment of the contraction level, and its theoretic...
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Báo cáo hóa học: " A note on the complete convergence for sequences of pairwise NQD random variables" potx
... When these are compared with the corresponding results of independent random variable sequences, there still remains much to be desired The concept of complete convergence of a sequence of random ... for the strong stability of Jamison’s weighted sums But most of their results were achieved under the identically distributed condition and some results were obtained even under the condition of ... Baum and Katz complete convergence theorem for pairwise NQD random variable sequences still holds true under the stronger condition The result generalizes and extends the corresponding result of...
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Báo cáo hóa học: " A note on the complete convergence for arrays of dependent random variables" docx
... P(|Xni | > δ) an n∈A i=1 The last inequality follows by J ≥ and the definition of A’ Hence, condition (ii) of Theorem 2.4 holds Finally, it remains to show that condition (i) of Theorem 2.4 holds That ... i=1 The rest of the proof is same as that of Theorem 1.6 except using Theorem 2.6 instead of Theorem 2.4 and is omitted ■ Acknowledgments The author would like to thank the referees for the helpful ... i=1 Hence, the result follows by conditions (i) and (ii) ■ Remark 2.7 As noted in the Introduction, Chen et al [9] have proved Theorem 2.6 under an additional condition (see also Theorem 1.5)...
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Báo cáo hóa học: " Research Article On the Existence Result for System of Generalized Strong Vector Quasiequilibrium Problems Somyot Plubtieng and Kanokwan Sitthithakerngkiet" doc
... Show that the set of solutions of SGSVQEP is closed Let { xα , uα : α ∈ I} be a net in the set of solutions of SGSVQEP such that xα , uα → x∗ , u∗ By definition of the set of solutions of SGSVQEP, ... important to study the existence of strong solution and properties of the strong solution set In general, the ideal solutions not exist Very recently, the generalized strong vector quasiequilibrium ... generalized monotone bifunctions,” Journal of Optimization Theory and Applications, vol 92, no 3, pp 527–542, 1997 Fixed Point Theory and Applications G Debreu, “A social equilibrium existence theorem,”...
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Báo cáo hóa học: " Experimental investigation on the bi-directional growing mechanism of the foils laminate approach in AAO fabrication" pptx
... appreciate one of reviewers’ comments that the bi-directional growth results from the non-uniform anodization along the sample surface Fast anodization of selected areas results in the formation of leakage ... the rate of alumina production on the metal side, the thickness of the barrier layer remains constant It can be further inferred from the experimental results that the anodization process along ... photolithographic patterned on one of the aluminum foils (Fig 6) serves as the gasket The other aluminum foil is electrolytically polished to assure the flatness of the contact surface Since the JSR is an elastic...
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Báo cáo hóa học: " Bounds on the Outage-Constrained Capacity Region of Space-Division Multiple-Access Radio Systems" docx
... (h2 ), and C12 (H) are the same as those in (8); they jointly define the multiple-access capacity region under the fading condition H As a result of the definition, the rate pairs in B1 (p) and B2 ... and B2 (p) only satisfy the constraint on the individual rates R1 and R2 , respectively; the rate pairs in Ba (p) only satisfy the constraint on the sum rate R1 + R2 We can now prove the following: ... series of planes rather than straight lines, and is therefore in the shape of a polyhedra instead of a polygon as in the two-user case Each plane will correspond to an outer bound on the capacity of...
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Báo cáo toán học: "On the Excluded Minors for Matroids of Branch-Width Three" ppsx
... T being the other component of T − e, the sets (T ), (T ) form a partition of (T ).) The width of the branch-decomposition (T, τ ) is the maximum of the widths of the edges of T , and the branch-width ... section we focus on matroid connectivity, and on branch-decompositions We mostly follow the definitions and concepts from [6] Let M be a matroid on the ground set E = E(M) The connectivity function ... from graph-theoretical and computational points of view This attention followed the pioneer work of Robertson and Seymour on the Graph Minor Project, and results of various researchers concerning...
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Báo cáo toán học: "On the Doubly Refined Enumeration of Alternating Sign Matrices and Totally Symmetric Self-Complementary Plane Partitions Tiago Fonseca" ppsx
... equality In conclusion, all these variations ((A.3) and (A.4)) are truly the same, and we can concentrate on only one version A.2 The conjecture in terms of TSSCPPs Mills, Robbins and Rumsey conjectured ... then shows that the contribution to the partition function 1/2 of any configuration is of the form zi times a polynomial of zi of degree at 1/2 most n − Summing over all configurations and removing ... satisfy the extra-step rules we can only build two type of islands, one made of r double vertical steps and s double diagonal steps, and the other type made of t vertical-diagonal steps and u diagonal-vertical...
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Báo cáo khoa học: "Comparative study on the aflatoxin B1 degradation ability of rumen fluid from Holstein steers and Korean native goats" pot
... Dilutions were performed in sterilized distilled water for preparation of the working solution, the concentration of which was further diluted in order to be within the detection range of the kit ... ragwort containing PA demonstrated vastly different quantities of plant material required to manifest clinical symptoms The consumption at the rate of more than 200% of the body weight of sheep and ... degradation ability of steer and goat conditions for a control Experiment B: Effect of rumen fluid collection time on aflatoxin degradation The effect on AFB1 degradation of rumen fluid collected at different...
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Báo cáo y học: "Early and mid term mortality after coronary artery bypass grafting in women depends on the surgical protocol: retrospective analysis of 3441 on- and off-pump coronary artery bypass grafting procedures" doc
... the preferred revascularization technique among female patients Authors’ contributions B.24.1 SE, EK, GJ, BR, PL have made substantial contributions to conception and design, or acquisition of ... M, et al: Estimation of contribution of changes in coronary care to improving survival, event rates, and coronary heart disease mortality across the WHO MONICA Project populations Lancet 2000, ... longer intensive care stay and therefore, a higher risk of pulmonary infection [14] Page of In conclusion, female gender seems to be a significant risk factor in many multivariate analyses Therefore,...
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Báo cáo y học: "An MRI study on the relations between muscle atrophy, shoulder function and glenohumeral deformity in shoulders of children with obstetric brachial plexus injury" ppt
... result of long standing lack of sufficient passive stretch[8] If correct this hypothesis would suggest that preserving passive range of motion and prevention of internal rotation contracture of the ... in the first period of life, the rate of deformation reducing in later years This suggests that prevention of deformities should focus on the first year of life Conclusion Another advantage of ... to: the pattern of atrophy, and the relation between muscle atrophy and both passive and active shoulder function Pattern of atrophy No consistent pattern of atrophy was found: the extent of atrophy...
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