mg 2 f determination from magnesium green fluorescence in the extramitochondrial volume of isolated mitochondria
Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... positions and of the EF-hand motif significantly compromised the metal-binding capability of the engineered protein, indicating that the metal binding of CD2.STIM1.EF is through the EF-hand motif from ... between the two termini of the inserted Ca2+-binding sites in the model structure of the EF-hand of STIM1 is within ˚ 15 A Accordingly, a total of six glycine linkers is sufficient to enable the grafted ... ability of the grafted EF-hand of STIM1 (B) Modelled structure of the engineered protein with the grafted EF-hand Ca2+-binding motif (magenta) from STIM1 W 32 and Y76 in the ˚ host protein are...
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Báo cáo khoa học: The signature amidase from Sulfolobus solfataricus belongs to the CX3C subgroup of enzymes cleaving both amides and nitriles Ser195 and Cys145 are predicted to be the active site nucleophiles pot
... One of its amino ˚ groups is about 2. 6 A from the Oc atom of Ser171 ˚ and 2. 5 A from the thiol group of Cys145 4 720 Even taking into account all of the limitations of molecular models of proteins ... analysis of the activity of the mutant SsAH-K96R on aliphatic amides and nitriles strengthens this hypothesis Another line of evidence comes from the results of the inhibition studies shown in Fig ... Ser171 and 2. 5 A from the amino group of Lys96 (Fig 4) This putative alternative triad is located in a different plane from the active site FEBS Journal 27 2 (20 05) 4716–4 724 ª 20 05 FEBS CX3C signature...
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Báo cáo khoa học: The role of the Fe-S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii potx
... is the purification of an AnfA variant reported by Austin et al [22 ] In their study, the N-terminal domain of AnfA was truncated to prevent the intrinsic aggregation of the intact form during ... holo-form is the other major difference, although this was hardly observed in the apo-form Regarding the effect of AMP-PNP on the proteolysis of VnfA, a similar effect of the nucleotide binding was ... oxygen, the vanishment of the Fe-S cluster from purified VnfA could be a result of the disassembly of the cluster during aerobic purification Fe-S clusters in FEBS Journal 27 7 (20 10) 817–8 32 ª 20 10 The...
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Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot
... protein composed of the N-terminal domain of hoPL and of the C-terminal domain of hoPLRP2 was named NcC2 Conversely, the other chimeric protein, bearing the N-terminal domain of hoPLRP2 and the ... used for N2C2 and N2Cc In conclusion, the accessibility of the active site was better in the protein bearing the N2 domain than in the protein bearing the Nc domain Thus, the accessibility of the ... Nevertheless, the mechanism of activation of PLRP2, which involves the C2 domain, is different from that of PL, which involves the Cc domain and colipase The C-terminal domain alone is involved in the affinity...
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Báo cáo khoa học: Identification and characterization of important residues in the catalytic mechanism of CMP-Neu5Ac synthetase from Neisseria meningitidis potx
... different Neu5Ac concentrations in the presence or absence of 0 .2 mm dithiothreitol Effect of Mg2 + concentration The effect of an increase in the concentration of Mg2 + was determined by measuring ... Arg165 Arg2 02 Lys1 42 Asn175 Asn2 12 Gln203 Gln166 Phe176 Arg165 Arg2 02 Lys1 42 Asn175 Asp245 Asn2 12 Asp211 Asp209 Asp245 Asp247 Asp211 Asp209 Asp247 Fig Cross-eye stereo-view of the residues of interest ... concentration of mm Because mutation of metal-binding residues would be expected to reduce the affinity of the enzyme for Mg2 +, we decided to investigate the effect of increasing the Mg2 + concentration...
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Báo cáo khoa học: Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis pdf
... Phorbol- 12- myristate-13-acetateinduced protein BCL -2- binding component-3 proteins inhibits the antiapoptotic activity of Bcl -2 by initiating the release of cytochrome c from mitochondria into the cytosol and increasing the ... sequestering Beclin away from hVps34; and (b) by reducing the affinity of UVRAG for Beclin and stabilizing the Beclin dimer (Fig 2) Rapid induction of autophagy regardless of Bcl -2 and Bcl-xL expression ... similarities to the pore-forming domains of the bacterial toxins colicins and diphtheria toxin [21 ,71] Moreover, Bcl -2 and Bcl-xL were shown to be capable of forming ion-conductive channels in synthetic...
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Báo cáo khoa học: Determination of the metal ion dependence and substrate specificity of a hydratase involved in the degradation pathway of biphenyl/chlorobiphenyl pot
... order of effectiveness: Mg2 +, Mn2+, Co2+, Zn2+ and Ca2+ This differs from the metal activation profile reported for MhpD, which is 1.6-fold more active with Mn2+ than with Mg2 + [7] In addition, the ... activity of the purified enzyme in the presence of saturating amounts of Mg2 + is 533 U mg) 1 The relative specific activity of BphH was tested with different divalent cations (Fig 3) CuCl2 and Fig pH ... Journal 27 2 (20 05) 966–974 ª 20 05 FEBS Characterization of BphH measured the dissociation constants of Mg2 + and Mn2+ and the in uence of each metal cofactor on the kinetic parameters for HPDA hydration...
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Báo cáo Y học: Barley a-amylase Met53 situated at the high-affinity subsite )2 belongs to a substrate binding motif in the bfia loop 2 of the catalytic (b/a)8-barrel and is critical for activity and substrate specificity pot
... Other binding residues (W9AMY2, H92AMY2, T94AMY2, A95AMY2, Y130AMY2, A145AMY2, F1 80AMY2, K182AMY2, W206AMY2, S208AMY2, Y211AMY2, H288AMY2, Q294AMY2, M296AMY2 and Q35TAA, H 122 TAA, R204TAA, K209TAA, ... [14,18,19 ,22 ,24 ,27 ] Glucosyl O6 at subsite )2 in enzyme/oligosaccharide complexes was hydrogen bonded to NE1 of the indole ring of Trp [7,17 ,20 ,21 ,23 ,25 ] or to NE2 of the carboxamide group in Gln ... residue in catalysis SD of Met52AMY2 (Met53AMY1) in a computed AMY2/malto decaose complex was 3.4 A from O6 of glucose at subsite )2 [45] reminiscent of the enzyme/substrate interaction for the corresponding...
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báo cáo sinh học:" National trends in the United States of America physician assistant workforce from 1980 to 2007" ppt
... equivalents) in the USA [24 -30] The IPUMS data for the 1980, 1990, and 20 00 are from the 'long form' samples of the U.S decennial Census in those years The IPUMS data for 20 05 and 20 07 are from the annual ... 1980 1990 20 00 20 05 20 07 20 24 0 (69.5) 13 6 62 (57.8) 21 990 (38.6) 30 21 8 (36.8) 36 923 (37.8) 35-44 5 020 (17 .2) 6985 (29 .6) 17 663 (31.0) 23 20 5 (28 .3) 29 3 02( 29.9) 45-54 21 60 (7.4) 20 28 (8.6) ... supported training of health professions in medicine and dentistry [6-8] The first period, from 1963 to 1975, appeared to lead the emergence of the PA profession Title VII support in the Washington...
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Energy Storage in the Emerging Era of Smart Grids Part 2 pot
... 5) C5 66 F (22 F × 3) C6 22 F C7 44 F (22 F × 2) C8 66 F (22 F × 3) Table Capacitance of each capacitor A photograph of a 20 0 W prototype is shown in Fig 11(a) N-channel MOSFETs with low ... shown in Table Multiple ceramic capacitors were connected in parallel to satisfy the required capacitance Capacitor Capacitance C1 22 F C2 44 F (22 F × 2) C3 44 F (22 F × 2) C4 110 F (22 F ... System 33 The discharging characteristics of an SC when using the discharger shown in Fig are illustrated in Fig 10 At the beginning of the discharging process of the SC, S0 is turned on and the SC...
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báo cáo hóa học: " Selective COX-2 inhibition prevents progressive dopamine neuron degeneration in a rat model of Parkinson''''s disease" potx
... effect F1 , 32 = 7, p = 0.01) This effect of selective COX -2 inhibition was more pronounced in the striatum at the 12- day time point after the toxin injection than at 21 days after 6-OHDA (Fig 2A,2B,2C) ... examined the effect of celecoxib on the DA system We analyzed the striatal volume of TH+ fibers (Fig 1B,1C,1D) and quantified the number of TH+ cell bodies in the SN (Fig 1E) At 12 days there ... adverse effects of conventional NSAIDs, which inhibit both isoforms of COX (constitutive and inducible) COX -2 is induced by proinflammatory stimuli and cytokines [25 ] Inhibition of the inducible form...
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Báo cáo hóa học: " Contribution of cysteine residues in the extracellular domain of the F protein of human respiratory syncytial virus to its function" docx
... residues 21 2, 3 82, and 422 did not disrupt folding sufficiently to affect processing of F0 to F1 and F2 Mutation of residues 69 and 322 dramatically reduced the levels of total protein immunoprecipitated ... 3 82- 393 37-439 313-343 416- 422 358-367 F2 -F1 monomer 69 -21 2 Homotrimer HR1 HR2 Figure Computer model of the HRSV F protein Computer model of the HRSV F protein The molecular structure of HRSV F ... 136/137 37 69 HR2 TM CT 20 7 21 2 **** **** *** 313/ 322 /333/343 4 82 416/ 422 /439 520 * 548 525 574 550 358/367/3 82/ 393 Figure Diagram 1of the HRSV F protein Diagram of the HRSV F protein A linear representation...
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Charade of the Debt Crisis From Buffoonery to Tragedy in the Debt Folly and Euro Farceby pptx
... reversed in the banking industry The same is true of the reasons for saving the biggest clods from their self-caused wounds In the wake of the financial flap, the deadbeats balked at fulfilling their ... down the drain Instead, the main reason for the jitters of the investing public lay in the lack of certainty concerning the bond market A second, and related, factor stemmed from the swirl of deceit ... crisis of 20 08 For one thing, each of the sovereign states opted to prop up the bloated banks caught up in the housing bubble After the orgy of mindless lending during the run-up to the financial flap,...
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Báo cáo sinh học: " From bending DNA to diabetes: the curious case of HMGA1" pps
... state, insulin action dominates, with RBP opposing insulin signaling in skeletal muscle (b) In states of Hmga1 deficiency, both downregulation of insulin receptor expression, promoting insulin resistance, ... Germane to this is the finding that the generalized IR of humans with INSR mutations is biochemically distinct both from the IR of patients with other monogenic forms of severe IR and from ‘common’ ... between the species The earlier failure of insulin secretion in mice than humans suggests a failure of glucose-stimulated insulin secretion in individual pancreatic β cells and/or failure of proliferation...
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Báo cáo khoa học: "Immunohistochemical Localization of Bcl-2 in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis" docx
... ether anesthesia, and the spinal cords were removed and frozen in a deep freezer (-70 C) for protein analysis Pieces of the spinal cords were processed for paraffin embedding after fixation in ... Laboratories Inc, San Francisco, CA) according to the manufacturer’ instructions s In brief, 5-m-thick sections of paraffin-embedded spinal cords were deparaffinized and treated with 0.3% H2O2 in methyl ... expression of Bcl -2 in EAE by western blot might come from the less immunoreactivity of Bcl -2 in host cells, or the amount of Bcl -2 expression in normal spinal cords overwhelm those of both host...
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Báo cáo khoa học: "Increased phosphorylation of c-Jun NH (2)-terminal protein kinase in the sciatic nerves of Lewis rats with experimental autoimmune neuritis" ppt
... deziniffarap-ed erew star detceffa-NAE dna lortnoc morf sevren citaics fo )mµ 5( snoitces eussit niffaraP yrtsimehcotsihonummI tnacifingis deredisnoc erew 50.0 naht ssel seulav htiw secnereffid ... lacofnoc resal 005VF na htiw denimaxe erew snemiceps deniats -ecnecseroulfonummi elbuod ehT SBP ot denruter dna ,O2H dellitsid ni niaga ylfeirb deppid ,nim 02 rof )0.5 Hp ,4HNOOC3HC Mm 05( reffub ... eht hcihw retfa ,nim 02 rof mpr 000 ,21 ta degufirtnec dna sebutorcim ot derrefsnart erew setanegomoh ehT rezinegomoh a ni sekorts 02 htiw )nitpepuel lm/gµ 01 ,ninitorpa lm/gµ 01 ,FSMP Mm ,4OV3aN...
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báo cáo khoa học: " Whole-Organ analysis of calcium behaviour in the developing pistil of olive (Olea europaea L.) as a tool for the determination of key events in sexual plant " ppsx
... to follow the dynamic of free calcium ions in the olive pistils, the fluorescent indicator Fluo-3 AM was injected directly into the inflorescences To confirm the presence of the incorporated Fluo-3 ... this stage, the main task of the flower bud is to complete the growth and maturation of anthers and the pistil Consequently, the intensity of the signalling events in the stigma of the flower bud ... http://www.biomedcentral.com/1471 -22 29/11/150 Page of 12 Figure Subcellular localization of Ca2+ in the stigmatic surface of developing olive pistils (A) Stigmatic surface of the pistil enclosed in a green flower bud...
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Báo cáo y học: " Ethnic differences in the adaptation rate of HIV gp120 from a vaccine trial" ppsx
... selection pressure either because of genetic differences in the magnitude, specificity, or potency of the natural immune response, or because of differences in factors affecting virus replication, ... whether levels of selection were significantly different between vaccinated and placebo individuals in different races, we analyzed clones per individual from 345 infected North Americans from the ... have been pointed out by the rgp 120 HIV Vaccine Study Group [3] as one of the potential factors to explain vaccine efficiency differences between white and non-white volunteers in the VAX004 trial...
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Báo cáo y học: " Modulation of the virus-receptor interaction by mutations in the V5 loop of feline immunodeficiency virus (FIV) following in vivo escape from neutralising antibody" ppsx
... at the other extreme, B25 42 and PPR are capable of infecting via an interaction with CRD1 alone [27 ,37] Preliminary analyses of the modes of CD134 interaction of diverse strains of FIV have indicated ... (p.m.) from a cat (A613) infected for 322 weeks with the 414 molecular clone of FIVGL8[41] A613 was one of a group of specific pathogenfree cats infected with the 414 clone Following infection, the ... Upregulation of surface feline CXCR4 expression following ectopic expression of CCR5: implications for studies of the cell tropism of feline immunodeficiency virus J Virol 20 02, 76: 924 2- 925 2 36 Willett...
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Báo cáo y học: " The role of the humoral immune response in the molecular evolution of the envelope C2, V3 and C3 regions in chronically HIV-2 infected patients" pdf
... PTHCC19 20 03 PTHCC20 1998 PTHSM2 20 02 PTHSM3 PTHSM6 PTHSM7 PTHSM9 1993 20 01 1996 1996 PTHSM10 20 01 20 03 20 05 20 03 20 03 20 03 20 04 20 03 20 05 20 03 20 05 20 03 20 04 20 05 20 03 20 03 20 04 20 03 20 04 20 05 20 03 ... na 3 42 265 21 2
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