memory cognitive and psychologicalfunctions of sleep update from electroencephalographic and neuroimaging studies
Subjective memory complaints and cognitive performance in a sample of healthy elderly pot
... caregivers of demented patients followed at the Behavioral and Cognitive Neurology Unit of the Faculty of Medicine of the Federal University of Minas Gerais, in Belo Horizonte (MG), Brazil, and also ... greater risk of future cognitive decline.2 Indeed, the diagnosis of mild cognitive impairment (MCI), which entails an increased likelihood of conversion to dementia, demands the existence of SMCs, ... difference in the performance of elderly subjects with and without SMCs on a series of brief cognitive tests assessing attention, episodic and semantic memory, and executive functions Only a...
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Báo cáo y học: "What determines the evolution of early undifferentiated arthritis and rheumatoid arthritis? An update from the Norfolk Arthritis Register" ppt
... with radiographs of their hands and feet taken within 12 months of symptom onset to be higher than the predictive value of RF with respect to both the development and progression of erosions [21] ... there was no evidence of any overall increased risk of cancer in the early years of IP and RA in NOAR, there was a definite increased risk of developing non-Hodgkin’s lymphoma (standardised incidence ... genes that affect the severity of the disease and genes that affect the handling of the drug Then there are psychosocial factors such as adherence to and expectations of therapy outcome Finally,...
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Characteristics of hydrogen production from food waste and waste activated sludge
... was supplemented with 200 mg of KH2PO4, 14 mg of MgCl2•4H2O, mg of Na2MoO4•4H2O, mg of CaCl2•2H2O, 2.5 mg of MnCl2•6H2O, and 10 mg of FeCl2•4H2O, which was modified from Lay et al (1999) NaHCO3 ... produce moles of hydrogen with mole of n-butyrate or moles of hydrogen with mole of acetate from mole of hexose In most cases using soluble defined substrates, hydrogen production yield and major ... M1-0203-00-0063 from the National Research Laboratory Program of the Korean Ministry of Science and Technology REFERENCES APHA, AWWA, WEF, 1998 Standard Methods for the examination of Water and Wastewater,...
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Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf
... sm The points from N-terminal and C-terminal residues (Arg1–Leu7 and Ala60–Gln67), together with those from Arg58 and Gln59 in the C-terminal end of helix III, are located apart from the major ... of a hinge point at Arg53 that exhibits conspicuous minima of J(0) and J(xN) and a significant maximum of J(0.87xH), and that emerges not only within helix III, but also over a large portion of ... S1 Bar graph of R1, R2 and {1H}–15N NOE values along the sequence of the TTF1 HD at 11.7 T and 286 K 448 Fig S2 Plot of J(0.87xN)–J(0) correlation from 15N relaxation measurements of the TTF-1...
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Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx
... s)1, Km of 0.148 ± 0.028 mm and kcat ⁄ Km of 5.2 · 104 m)1Æs)1 toward shikimate, and a kcat of 7.1 ± 0.7 s)1, Km of 0.182 ± 0.027 mm and kcat ⁄ Km of 3.9 · 104 m)1Æs)1 toward NADP Different from ... profile of HpSDH It is found that the pH optimum of 4686 Fig Temperature profile of HpSDH enzyme activity Fig pH profile of HpSDH enzyme activity HpSDH is between 8.0 and 9.0, and the pH optimum of ... b-sheet, b-turn, and random coil in HpSDH are, respectively, 16.6, 49.2, 1.5, and 32.6% processed by jasco secondary structure estimation software The percentage for random coil of HpSDH is similar...
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Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx
... sequences of VsEndA (Fig 1) The theoretical pI was 9.61 for VsEndA and 8.62 for VcEndA Expression and purification From L Escherichia coli culture, a total of 24 and 50 mg pure recombinant VsEndA and ... Endonuclease I from V salmonicida and V cholerae Here we report the cloning, expression and purification of the endonuclease I enzymes from the psychrophile V salmonicida (VsEndA) and the mesophile ... characterization of the enzymes, we carried out an analysis of the NaCl requirements and pH optimum of VsEndA and VcEndA The optimum NaCl concentrations for activity were found to be 425 mm for VsEndA and...
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... by the patterns of the product formation, the release of dimers, trimers and tetramers from the hexamer was considered to result from direct action of the enzymes On the other hand, the pentamer ... (Fig 4A) In the case of the E315Q mutant, an additional faint band was also seen at an Mr of 43 000 This band appeared as a degradation product during freezing and thawing of the protein that ... species of hydrolytic products, varying from GlcNAc to (GlcNAc)6 After adjusting the concentration of the enzymes to yield similar activity, the hydrolytic activities of the wild-type protein and of...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx
... and pufferfish (torafugu and spotted green pufferfish) IL-10 sequences Pufferfish and carp IL-10 genes, clustered together and distant from IL-20 and IL24, as recently determined from analysis of ... 5¢-ACTACCTCATGAAGATCCTG-3¢ and reverse: 5¢-TTGCTGACCACATCTGCTG-3¢) served as a control for the quantity and quality of cDNA Semiquantitative analysis of RT–PCR products Suspensions of Carp head kidney and liver ... (AAG41401), IL-26 (NP_060872) and interferon-a (P01579); from torafugu IL-10 (CAD62446); from spotted green pufferfish IL-10 (CAD 67773), IL-20 (AY294557) and IL-24 (AY294560); and from carp IL-10 (AB110780)...
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Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc
... mapping On the basis of the assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (kint) in the productive complex is independent of the length of the substrate, Km and Vmax were used ... pET24d from Novagen (Madison, WI, USA) PGA was obtained from ICN (Zoetermeer, the Netherlands) Saturated oligoGalpA (DP 28) and unsaturated oligoGalpA (DP 37) were prepared and puried from polygalacturonase ... values A-1 and kint were derived from a plot of exp(An+1 RT) against (1 kcat)n: expAnỵ1 =RTị ẳ ẵkint =kcat ịn expA1 =RTị in which A-1 and kint are determined from the vertical and horizontal...
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Tài liệu Báo cáo khoa học: "Extraction and Approximation of Numerical Attributes from the Web" pdf
... Usage of all this knowledge allows us to select the correct value of 1.695m and reject other values Thus we can increase the precision of value extraction by finding and analyzing an entire group of ... the acquisition of numerical attributes from the Web and attempt to deal with ambiguity and noise of the retrieved attribute values (Aramaki et al., 2007) utilize a small set of patterns to extract ... the textual context of these values A significant body of recent research deals with extraction of various data from web tables and lists (e.g., (Cafarella et al., 2008; Crestan and Pantel, 2010))...
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Tài liệu Báo cáo khoa học: Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt
... appropriate amount of enzyme at 30 °C The reducing sugar liberated by hydrolysis of CMC was determined by the method of Nelson and Somogyi [25] One unit of cellulase was defined as the amount of enzyme ... Construction of the cDNA library and cloning of cellulase cDNA was achieved as follows: Total RNA was extracted from g of abalone hepatopancreas by the ganidinium thiocyanate-phenol method [30] and mRNA ... reverse primers R1 and R2 were synthesized on the basis of sequences of LP5 and LP6, respectively (Table 2) PCR amplification of HdEG66-cDNA cDNAs encoding the N-terminal region of the HdEG66 were...
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Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt
... fluorescence intensity as function of time of doublelabeled liposomes made from PS (A) or from PC (B) after the addition of MfGl-II or LPS Re at t = 50 s and subsequent addition of LBP (0.2 mM) at t = 100 ... behavior of the acyl chains was derived from the peak position of the symmetric stretching vibration of the methylene groups ms (CH2), which lies around 2850 cm)1 in the gel and between 2852 cm)1 and ... of unlabeled molecules into the double-labeled liposomes leads to probe dilution and with that to a decrease in the efficiency of FRET: the emission intensity of the donor increases and that of...
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Tài liệu Báo cáo khoa học: Dimer asymmetry and the catalytic cycle of alkaline phosphatase from Escherichia coli doc
... phosphate-binding and calorimetric studies suggested positive cooperativity of Zn2+ binding to the M1 sites of the dimeric APase [5] NMR studies indicate that metal ion migration from the M1 site of an inactive ... enzyme Influence of Zn2+ on the kinetic parameters and the deviations from Michaelis–Menten kinetics A vast amount of data indicates that the subunits of the homodimeric APase from E coli often not ... curve-fitting procedure and describing the affinity of the subunits for the substrate (KS1 and KS2) and for Pi (KI1 and KI2), presented in Table and Table 2, respectively, are independent of the Zn2+ ion...
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Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of flavonol synthase from Citrus unshiu pptx
... evolved from a common ancestral gene, and the essence of most of the conserved amino acids has been further substantiated by site-directed mutagenesis of FHT [7,9] and by documentation of ligand ... initially from Ruta species Flavonols originate from flavanones, i.e (2S)-naringenin, by the consecutive action of FHT and FLS (Fig 1), and both of these enzymes use molecular oxygen for catalysis and ... functionally proved and the lability of substrate and product rule out any cocrystallization The setting of a helices and b-strands causes very similar CD spectra for this class of enzymes [27–29],...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... absence of nucleotides (Table 1) The rate of CTP synthesis was dramatically inuenced by the presence of GTP, and 20 and 41-fold increases in kcat were obtained with GTP concentrations of 0.1 mM and ... basis of the kinetic constants in Table 1, and those obtained for the glutaminase reaction in the presence of 0.1 mM each of UTP and ATP-cS (data not shown) Apparently, the value of Ka, kcat,1 and ... activation of the glutaminase activity in the presence of 0.1 mM (open circles) or mM (closed circles) each of UTP and ATP-cS (C) GTP activation of CTP synthesis at 0.1 mM each of UTP and ATP L...
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Tài liệu Determining the Cost of Producing Ethanol from Corn Starch and Lignocellulosic Feedstocks pot
... Cost of Producing Ethanol from Corn Starch and Lignocellulosic Feedstocks A Joint Study Sponsored by: U.S Department of Agriculture and U.S Department of Energy Andrew McAloon, Frank Taylor, and ... Available for a processing fee to U.S Department of Energy and its contractors, in paper, from: U.S Department of Energy Office of Scientific and Technical Information P.O Box 62 Oak Ridge, TN ... Office of Energy Policy and New Uses Oak Ridge National Laboratory U.S Department of Agriculture iv I Introduction The U.S Department of Energy (DOE) is promoting the development of ethanol from...
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Tài liệu Interactions Between Workers and the Technology of Production: Evidence from Professional Baseball doc
... 1613-1634 [3] Edin, Per-Anders, Peter Fredriksson, and Olof Åslund, “Ethnic Enclaves and the Economic Success of Immigrants — Evidence from a Natural Experiment,” Quarterly Journal of Economics, CXVIII ... the University of Bonn and offers a stimulating research environment through its research networks, research support, and visitors and doctoral programs IZA engages in (i) original and internationally ... research in all fields of labor economics, (ii) development of policy concepts, and (iii) dissemination of research results and concepts to the interested public IZA Discussion Papers often represent...
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Cognitive and Physiologic Correlates of Subclinical Structural Brain Disease in Elderly Healthy Control Subjects docx
... among increasing age and increasing volume of SSBD (a), increasing volumes of SSBD and poor cognitive performance (b), reduced connectivity and cognitive impairment (c), and increasing disconnection ... RELATIONSHIP OF SSBD AND FUNCTIONAL CONNECTIVITY, AND OF CONNECTIVITY WITH COGNITION Increasing PVH volumes were associated with significantly lower values of coherence in prerolandic and postrolandic ... between the genu and splenium of the corpus callosum, drawn where that distance was smallest The use of the MRX software package has been investigated by Sandor and colleagues,40 and Guttmann et...
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Báo cáo khoa học: Crystal structures of the apo form of b-fructofuranosidase from Bifidobacterium longum and its complex with fructose pot
... structure of B longum b-fructofuranosidase domains involve contacts of IV and V blades and b-strands (1–6) from the internal b-sheet of the b-sandwich domain The last four amino acids of the native ... hydrodynamic radius ˚ ˚ of 16.78 A, average radius of 34.02 A and ratio of the shortest to the longest axis of 0.628 Kinetic parameters of b-fructofuranosidase The activity of b-fructofuranosidase was ... expression and purification of recombinant b-fructofuranosidase The high-resolution structure of b-fructofuranosidase from B longum is the first one for an enzyme of the GH32 family from nonsporulating...
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Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot
... far- and near-UV CD spectra of Tma-RNase HI, Tma-CD and Tma-ND were measured at 20 °C and pH 9.0, and comparisons are shown in Fig The farand near-UV CD spectra of Tma-CD are similar to those of ... that removal of the HBD does not significantly affect the structure of the RNase H domain of Tma-RNase HI The far- and near-UV CD spectra of Tma-ND were significantly different from those of Tma-RNase ... the presence of mm MgCl2 or MnCl2 and compared with those of Tma-RNase HI (Table 1) The Vmax values of Tma-CD determined in the presence of mm MgCl2 and MnCl2 were 410-fold lower and 1.6-fold...
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