loop mediated isothermal amplification of dna

... diagnosis of Human Herpesvirus infection by a novel DNA amplification method, loop- mediated isothermal amplification J Clin Microbiol 2004, 42:140-145 Iwamoto T, Sonobe T, Hayashi K: Loop- mediated isothermal ... Notomi T: Loopmediated isothermal amplification reaction using a nondenatured template Clin Chem 2001, 47:1742-1743 Mori Y, Nagamine K, Tomita N, Notomi T: Detection of loopmediated isothermal amplification ... BIP, F3, KHV DNA showing the effect of for on amplification of Agarose3 by LAMP assay at 65°Ctime durations of 10–60B3, Agarose gel showing the effect of time on amplification of KHV DNA by LAMP...

Ngày tải lên: 19/06/2014, 08:20

... diagnosis of Human Herpesvirus infection by a novel DNA amplification method, loop- mediated isothermal amplification J Clin Microbiol 2004, 42:140-145 Iwamoto T, Sonobe T, Hayashi K: Loop- mediated isothermal ... Notomi T: Loopmediated isothermal amplification reaction using a nondenatured template Clin Chem 2001, 47:1742-1743 Mori Y, Nagamine K, Tomita N, Notomi T: Detection of loopmediated isothermal amplification ... BIP, F3, KHV DNA showing the effect of for on amplification of Agarose3 by LAMP assay at 65°Ctime durations of 10–60B3, Agarose gel showing the effect of time on amplification of KHV DNA by LAMP...

Ngày tải lên: 20/06/2014, 04:20

... transcription loop- mediated isothermal amplification (RT-LAMP) primer sets Primer F3 B3 FIP BIP LF LB Type Forward outer Backward outer Forward inner (F1c + F2) Backward inner(B1c + B2) Loop Forward Loop ... Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T: Loop- mediated isothermal amplification of DNA Nucleic Acids Res 2000, 28:63 Yang JinLong, Yang Rui, Cheng AnChun, Wang ... detection of Goose Parvovirus in the field by loop- mediated isothermal amplification Virol J 2010, 7:14 Ke GM, Cheng HL, Ke LY, Ji WT, Chulu JL, Liao MH, Chang TJ, Liu HJ: Development of a quantitative...

Ngày tải lên: 21/06/2014, 19:20

... N, Hase T: Loop- mediated isothermal amplification of DNA Nucleic Acids Research 2000, 28(12):e63 Dukes JP, King DP, Alexandersen S: Novel reverse transcription loopmediated isothermal amplification ... new method of nucleic acid amplication loop- mediated isothermal amplication of DNA Virologica Sinica 2008, 23(3):167-172 Fang Xue-en, wei Xiong, Li Jian, Chen Qin: Loop- mediated isothermal amplication ... 13 14 Page of loop- mediated isothermal amplification method Journal of Virological Methods 2007, 141:173-180 Rodríguez E, Betancourt A, Barrera M, Lee C, Yoo y D: Rapid detection of swine transmissible...

Ngày tải lên: 12/08/2014, 01:21

... N, Hase T: Loop- mediated isothermal amplification of DNA Nucleic Acids Res 2000, 28:63 Mori Y, Nagamine K, Tomita N, Notomi T: Detection of loop- mediated isothermal amplification reaction by ... T: Loop- mediated isothermal amplification reaction using a nondenatured template Clin Chem 2001, 47:1742-1743 Nagamine K, Hase T, Notomi T: Accelerated reaction by loop- mediated isothermal amplification ... by loop- mediated isothermal amplification J Virol Methods 2004, 115:59-65 Savan R, Igarashi A, Matsuoka S, Sakai M: Sensitive and rapid detection of edwardsiellosis in fish by a loop- mediated isothermal...

Ngày tải lên: 12/08/2014, 04:21

... triển phương pháp khuếch đại DNA điều kiện đẳng nhiệt (Loop mediated isothermal amplification – LAMP) với độ đặc hiệu, hiệu cao [72] Khác với phương pháp PCR, mạch đôi DNA không cần biến tính trình ... theo chu kỳ DNA hình tạ nhanh chóng chuyển sang dạng thân - vòng (stem - loop DNA) trình tự làm mồi tổng hợp DNA (bước 7) Sau đó, cấu trúc thân vòng làm khuôn cho bước khuếch đại DNA theo chu ... 14 1.4.2.3 Chức .15 1.4.2.4 Tiềm gen cpn60 .16 1.5 Phương pháp Loop mediated isothermal amplification (LAMP) 17 1.5.1 Sơ lược số phương pháp chẩn đoán tác nhân gây bệnh...

Ngày tải lên: 27/09/2014, 20:41

... Kb Kilo base Mb Mega base LAMP Loop- mediated isothermal Khuếch đại đẳng nhiệt có tạo amplification thành vòng OD Optical Density PCR Polymerase chain reaction rDNA Ribosomal Deoxyribonucleic ... pháp vi sinh hóa sinh 28 1.4.2 Phương pháp sinh học phân tử 29 1.5 KỸ THUẬT LAMP (Loop- mediated isothermal amplifications) 30 1.5.1 Giới thiệu kỹ thuật LAMP 32 1.5.2 Nguyên lý thiết kế mồi cho ... Phản ứng cắt DNA plasmid enzym hạn chế 56 2.2.2.7 Ghép nối đoạn DNA 57 2.2.2.8 Biến nạp DNA plasmid 58 2.2.2.9 Chọn lọc thể biến nạp mang plasmid tái tổ hợp 59 2.2.2.10 Tinh sản phẩm DNA plasmid...

Ngày tải lên: 11/07/2015, 01:15

... with organic load viii LIST OF FIGURES Figure Schematic diagram of loop- mediated isothermal amplification (LAMP) Figure Principle of loop mediated isothermal DNA amplification (LAMP) reaction ... Schematic diagram of loop- mediated isothermal amplification (LAMP) reactions (Adapted from Notomi et al., 2000) 16 2.3.3 Loop- mediated isothermal amplification with bioluminescence After DNA amplification, ... baseline threshold (Corless et al., 2000) 2.3.2 Loop- mediated isothermal amplification Loop- mediated isothermal amplification (LAMP) was developed by a group of scientists from Tokyo, Japan (Notomi et...

Ngày tải lên: 30/09/2015, 10:11

... Method of Nucleic Acid Amplification -Loop- mediated Isothermal Amplification of DNA - Motoki Goto, Eiichi Honda, Atsuo Ogura, Akio Nomoto, and Ken-Ichi Hanaki, 2009; Colorimetric detection of loopmediated ... Inoue, Loop- Mediated Isothermal Amplification of DNA (LAMP) General Introduction - Norihiro Tomita, Yasuyoshi Mori, Hidetoshi Kanda & Tsugunori Notomi, 2008; Loop- mediated isothermal amplification ... Kouichi Morita, 2006; development and evaluation of reverse transcription Loop mediated isothermal amplification assay for rapid and Real-time detection of japanese encephalitis virus - Xue-en FANG,...

Ngày tải lên: 13/10/2014, 20:25

... (2000) Loop- mediated isothermal amplification of DNA Nucleic acids Res 15: E63 25 Panangala VS, Shoemaker CA, Van Santan VL, Kleisius PH (2005), Analysis of 16S-23S intergenic spacer regions of the ... enteric septicemia of catfish, International J Systemc Bacteriol, 31:396-400 19 Iwamoto T, Sonobe T, Hayashi K (2003) Loop- mediated isothermal amplification for direct detection of Mycobacterium ... 281289 34 Yeh HY, Shoemarker CA, Klesius PH (2005), Evaluation of a loopmediated isothermal amplification method for rapid detection of channel catfish Ictalurus punstatus important bacterial pathogen...

Ngày tải lên: 25/11/2014, 21:42

... Page of 11 Figure Immunofluorescence 24 h after NP -mediated transfection pIRES-hrGFP-HMGB1 transfection with NP -mediated protocols PlanoAuNP (a, b, c), PLAL-AuNP Size (d, e, f), and MA Lipofection ... μL of each sized AuNPs were incubated for 24 h at room temperature with μg of pIRES-hrGFPII-eIL-12 or pIREShrGFPII-rHMGB1 at a total volume of 95 μL of ddH2O For transfection μL aliquots of FHD ... transfection: (MA Lipofection & MATra-A): 4.1) MA Lipofection: μL of FHD (Roche, Mannheim, Germany) were added to μg of pIRES-hrGFPII-eIL-12 or pIRES-hrGFPII-rHMGB1 to a total volume of 97 μL ddH2O...

Ngày tải lên: 11/08/2014, 08:20

... TABLE OF CONTENTS Acknowledgements i Summary ii Table of Contents iv List of Tables ix List of Diagrams ix List of Figures x Abbreviations xiv Chapter 1: 1.1 INTRODUCTION Historical Overview of DNA ... maintenance of the MDM2/p53 negative feedback regulatory loop 26 1.4.4 DNMT1 and DNA replication Earlier work on DNA methylation identified the recruitment of DNMTs to sites of active DNA synthesis ... slides along the DNA duplex during DNA replication (Krishna et al., 1994) The binding of PCNA to DNA apparently facilitates the recruitment of DNMT1 and methylation of hemi-methylated DNA in S phase...

Ngày tải lên: 14/09/2015, 14:13

... fragmentation, which is one of the most typical characteristics of apoptosis Regulators of DNA fragmentation One of the terminal processes of apoptosis is DNA degradation During apoptosis, DNA breakage usually ... at chromatin loop domains (50–300 kb) to generate high relative molecular mass DNA fragments; the second is cleavage of loose parts of internucleosomal DNA (in approximate multiples of 180 bp, ... tail Phosphorylation of H2AX on Ser139 is a key event in the repair of DNA damage and the induction of DNA degradation leading to cell death; therefore, the phosphorylated form of H2AX (cH2AX) is...

Ngày tải lên: 14/02/2014, 22:20

... blockade of DNA gyrase-induced supercoiling activity The observation that the antimicrobial effects of H4(86–100), HNr and the compound 3, like those of the quinolone class of antibiotics acting on DNA ... electrophoretic mobility of standard supercoiled pBR322 DNA (SS sample from Topogen) The migration of the small amount of relaxed DNA contained within this sample was also not affected by any one of these agents ... 3E,F) Time dependence of the bactericidal activity of H4-(86–100) and ciprofloxacin In order to evaluate the rapidity of action of histone H4 peptides and compare it with that of the quinolone antibiotic...

Ngày tải lên: 18/02/2014, 14:20

... Table Purification step of C cinereus DNA polymerase a One unit (1 U) of DNA polymerase was defined as the amount needs to catalyze the incorporation of pmol of [3H]-d TTP into a DNA polymer in 30 Protein ... of p140 and p48 from meiotic cells The expression profile of each subunit of DNA polymerase a-primase has been shown in mammalian somatic cells [13] and yeast [33,34] The transcripts of both DNA ... despite the lack of bulk DNA synthesis, we observed significant levels of expression of p140 and p48 in both the leptotene and zygotene stages (Fig 2) In both cases, the highest level of expression...

Ngày tải lên: 20/02/2014, 11:20

... methylation of one DNA domain whereas without the cooperation with Dnmt1, Dnmt3a would have to rebind to the DNA after each turnover to achieve complete methylation of one domain of the DNA It should ... for maintenance of the methylation at certain DNA sequences A role of Fig Stimulation of Dnmt1 and Dnmt3a by fully methylated CpG sites In this experiment the rates of methylation of the 310mer ... novo methylation of DNA in the absence of Dnmt1 Whereas the maintenance methylation is not an issue of our work, we present a mechanistic basis for the functional cooperation of Dnmt3a and Dnmt1...

Ngày tải lên: 21/02/2014, 01:21

... G-tetrad core (up–down–up–down) (E–G) Three types of loops (colored red): (E) diagonal loop, (F) edgewise loop and (G) double-chain-reversal loop Arrows indicate the strand orientations, from ... 2E); (b) edgewise loop (also called lateral loop) connecting two adjacent antiparallel strands (Fig 2F); and (c) double-chain-reversal loop (also called propeller loop or side loop) connecting ... the absence of looping constraints There are four medium-size grooves in such a structure For the d(TTAGGG) sequence, high concentrations of K+ and ⁄ or DNA favor the 3¢-end stacking of two such...

Ngày tải lên: 06/03/2014, 09:22

... selective excitation of Trp residues, or at 320 nm for excitation of essDNA For analysis of the quenching of Trp fluorescence, Mhr1– DNA complexes were formed by addition of 74-mer oligoDNA to 0.5 lm ... of Mg2+ Circular ssDNA of / X174 (10 lM) or circular dsDNA of pUC119 (5 lM) was incubated with the Mhr1 mutants (0.2, 0.4, 0.6, 0.8 and 1.0 lM for ssDNA; 0.1, 0.2, 0.3, 0.4 and 0.6 lM for dsDNA) ... Mg2+-dependent DNA- binding activity of Mhr1 Circular ssDNA of / X174 (5 lM) or circular dsDNA of pUC119 (10 lM) was incubated with 0.125, 0.25, 0.5 and lM Mhr1 in T buffer in the presence of 10 mM...

Ngày tải lên: 06/03/2014, 09:22

... characterizations of the mutant forms of the Tus protein, many aspects of the mechanism of replication fork arrest at Tus-Ter complexes have been determined This and a brief description of the current state of ... melting of the DNA catalyzed by DnaB and the flipping of C6 that causes strong affinity of Tus for Ter The helicase approaching the permissive face fails to induce high-affinity binding of Tus ... on DNA (double- or single-stranded) It is known that when encountering a linear DNA with a 5’ tail and 3’ blunt end, DnaB enters DNA with both strands passing through the central channel of DnaB...

Ngày tải lên: 06/03/2014, 23:20

... whatever DNA polymerases are used Expansion of DNA repeat sequences Selective binding of DDI-1A and DDI-1B to bulge DNA Because formation of the bulge structure might be important in DNA slippage ... conformational transitions of DNA directly and to eliminate drug interference, the CD spectra of native DNA and altered DNA, after subtracting the spectrum for the drug alone from that of the complex, are ... conformation because of an overall bending of the DNA backbone [33,35] Both DDI-1A and DDI-1B exhibited binding behaviors obviously different to that of the bulge DNA host The addition of DDI-1A and...

Ngày tải lên: 07/03/2014, 06:20