local nanomechanical motion of the cell wall of saccharomyces cerevisiae
Báo cáo Y học: A polymer with a backbone of 3-deoxy-D-glycero -D-galacto -non-2ulopyranosonic acid, a teichuronic acid, and a b-glucosylated ribitol teichoic acid in the cell wall of plant pathogenic Streptomyces sp. VKM Ac-2124 pdf
Ngày tải lên :
17/03/2014, 10:20
... percentage of the teichoic acid ( 10 % mass of the cell wall) was calculated from the content of the teichoic acidlinked phosphorus (0.8%) and taking into account the structure of the polymer (the ... %, and 10 % of the mass of the cell wall, respectively The three polymers altogether constitute 50 % of the mass of dry cell wall Thus, the present study shows that the Kdn-containing polymer, ... proves the position of the acetamido groups at C-2 and C-3 of this sugar The signal of the H-5 of this sugar appeared as a doublet, which suggests the absence of protons at H-6 In addition, the...
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Báo cáo khoa học: Inhibition of the D-alanine:D-alanyl carrier protein ligase from Bacillus subtilis increases the bacterium’s susceptibility to antibiotics that target the cell wall potx
Ngày tải lên :
30/03/2014, 16:20
... alanylated, which reduces or eliminates the negative surface charge of the bacterial membrane Reductions in the d-alanyl content of the cell wall influences directly the autolysis mechanism [2,10,11] ... 3) The Km value of DltA for d-Ala was subsequently determined by varying the d-Ala concentration between and 1000 lm The resulting Km of 13.62 ± 4.18 lm (Fig 3) is well in the range of the Km of ... if the inhibitor was able to penetrate the cell wall to reach its target DltA, we investigated changes in the growth rates of the Gram positive wild type strains B subtilis JH642 and the the...
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báo cáo khoa học: " Inorganic polyphosphate occurs in the cell wall of Chlamydomonas reinhardtii and accumulates during cytokinesis" docx
Ngày tải lên :
12/08/2014, 05:20
... be especially important during the delicate state of cytokinesis, when the protective cell wall of the mother cell is degraded and the cell wall of the daughter cells has not yet fully formed ... usually contained more mother cells at the end of cytokinesis Therefore, we tested whether the poly P content in the cell wall fluctuates during the cell cycle In a culture of asynchronously growing ... all of these criteria and is therefore considered to provide proof for the presence of poly P in the cell wall of this alga This conclusion was further confirmed by the complete absence of any...
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Tài liệu Báo cáo khoa học: Integral membrane proteins in the mitochondrial outer membrane of Saccharomyces cerevisiae docx
Ngày tải lên :
19/02/2014, 07:20
... related proteins from other fungi, or even in the fission yeast Schizosaccharomyces pombe Both the lack of obvious orthologs in other fungi and the abundance of Om14 in the outer membrane would ... identified in the Triton X-114 detergent phase from the mitochondrial outer membrane of Saccharomyces cerevisiae The details of mass spectrometry and N-terminal sequencing are provided in the Experimental ... regulation of the cytosolic NADH metabolism in the yeast Saccharomyces cerevisiae Mol Cell Biochem 256– 257, 73–81 33 Esser K, Jan PS, Pratje E & Michaelis G (2004) The mitochondrial IMP peptidase of...
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Tài liệu Báo cáo Y học: Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium pptx
Ngày tải lên :
22/02/2014, 04:20
... absent from the lane corresponding to the mls1D mutant (arrow; Fig 1A), thereby con®rming the speci®city of the antibody Application of the antibody to thin sections of wild-type cells grown ... acid medium [33], the issue of the partitioning of the glyoxylate cycle in cells grown under fatty acid-medium conditions has hitherto remained open We showed here that one of the key glyoxylate-cycle ... position [21] The signi®cance of this similarity is currently being addressed Demonstration of the cytosolic location of Mls1p in wild-type cells grown on ethanol completes the picture of the extra-peroxisomal...
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Báo cáo Y học: Expression of the V-ATPase proteolipid subunit of Acetabularia acetabulum in a VMA3-deficient strain of Saccharomyces cerevisiae and study of its complementation pdf
Ngày tải lên :
08/03/2014, 23:20
... for the subunits A and B of V-ATPase The intracellular localization of these isoforms and the combination of the isoforms is yet to be determined We are now trying to express fusion proteins of ... three isoforms of the subunit a of mouse enzyme [22,23], four isoforms of the proteolipid subunit in Caenorhabditis elegans [24] and two isoforms of the subunit A in human osteoclastoma [25] Isoforms ... although the difference was slight The respective gene was introduced into the S cerevisiae strain YN45 (VMA3 coding for one of the proteolipid subunits of V-ATPase is deleted) to examine whether the...
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Báo cáo khoa học: Mutational analysis of functional domains in Mrs2p, the mitochondrial Mg2+ channel protein of Saccharomyces cerevisiae ppt
Ngày tải lên :
23/03/2014, 10:21
... Three of them (CRB-3–5) are in the vicinity of two putative coiled-coil domains, which suggests that they may participate together with the coiled-coil domains in folding of the N-terminal half of ... domains may position them in a way that they can contribute to the formation of higher order structures of the Mrs2p part on the inner side of the membrane, which may contribute to the proposed opening ... feature of Mrs2p is the existence of two transmembrane-domains and a short connecting sequence of about 7–8 amino acids This is supposed to be the only part of the protein located in the intermembrane...
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Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx
Ngày tải lên :
23/03/2014, 11:20
... activity (Fig 1B) These rapid effects of d-glucose suggest action at the membrane level, possibly during the transport of glucose into the cell We therefore studied the effect of 2-deoxy-d-glucose, ... during the in vitro procedure, the amount of HAT in membranes from glucose-treated cells would be the same as that from glucose-untreated cells The possibility that the presence of glucose during the ... K+-ATPase in the plasma membrane of mammalian cells To confirm the interaction of acetylated tubulin with H+-ATPase and the presence of the H+-ATPase–acetylated tubulin complex in membranes of glucose-starved...
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Báo cáo khoa học: The N-acetylglutamate synthase/N-acetylglutamate kinase metabolon of Saccharomyces cerevisiae allows co-ordinated feedback regulation of the first two steps in arginine biosynthesis potx
Ngày tải lên :
23/03/2014, 21:20
... function of the yeast NAGS/NAGK metabolon We analyse (a) the role of the reductase in the activity and protein level of the synthase, (b) the role of the ASD of the kinase in its interaction with the ... reflecting inhibition of NAGK in the NAGS/NAGK complex The presence of NAGS had also the effect of rendering hyperbolic the inhibition of the kinase by arginine, whereas in absence of NAGS the inhibition ... high copy-number of ARG2 [32] Another remarkable result, concerning the regulation of the first enzymes of the arginine pathway, has been reported by the team of R L Weiss A series of ornithine-overproducing...
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Báo cáo Y học: Molecular characterization of MRG19 of Saccharomyces cerevisiae Implication in the regulation of galactose and nonfermentable carbon source utilization pdf
Ngày tải lên :
31/03/2014, 08:20
... that the energy status of the cell is an important determinant of GAL gene induction [15,25–27] This suggests that the availability of metabolic energy is a rate-limiting step in the synthesis of ... density Both of these promoters are under the control of the Adr1p transcriptional activator [44] b-galactosidase activity was the same in wild-type and the MRG19 disruptant regardless of the cell density ... repression of the SUC2 gene in Saccharomyces cerevisiae FEBS Lett 434, 71–76 Reinhardt, A & Hubbard, T (1998) Using neural networks for the prediction of the subcellular location of proteins...
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Báo cáo lâm nghiệp: "Relationships between the intra-ring wood density assessed by X-ray densitometry and optical anatomical measurements in conifers. Consequences for the cell wall apparent density determination" pps
Ngày tải lên :
08/08/2014, 01:22
... that the thicker S2 layer and the higher cellulose content of the LW cell wall account for the difference observed Stamm [37] confirms the increase of the cellulose content of the cell wall from ... length of (ring width/20); T = mean tangential size of the cell = width of the window of measurement / number of cells on the tangential width of the window of measurement; x = mean cell wall width, ... profiles according to the method used for the determination of the cell wall proportion (Fig 6) The effects of the treatments of the wood sample (embedding, slicing, drying…) and of the image of...
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Báo cáo y học: "What properties characterize the hub proteins of the protein-protein interaction network of Saccharomyces cerevisiae" pot
Ngày tải lên :
14/08/2014, 16:21
... be targeted The hubs of the PPIN of S cerevisiae have been shown to evolve slowly, which may be because larger portions of the lengths of these proteins are directly involved in their interactions ... hubs for the topology of the network [14] Further, while there is no substantial difference between the proportion of essential proteins among the party and date hubs, perturbation of the latter ... We used the computationally verified core data set [16] from the database of interacting proteins (DIP) [17] to build a representation of the PPIN of S cerevisiae The data set consists of 2,640...
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Identification and characterization of a candida albicans alpha 1,2 mannosyltransferase CaMNN5 that suppresses the iron dependent growth defect of saccharomyces cerevisiae aft1 delta mutant
Ngày tải lên :
16/09/2015, 08:30
... which the expression of CaMNN5 enhances the growth of S cerevisiae cells under iron-limiting conditions Also, the mechanisms underlying the phenotypes of Camnn5Δ are discussed Unlike in S cerevisiae, ... (Ramanan and Wang, 2000) 1.3.1 Adhesins The first step in the infection process is the adhesion of C albicans cells to epithelial and endothelial cells of the host, which is indispensable for colonization, ... glycosylation in S cerevisiae led to the isolation of a number of S cerevisiae mnn mutants, some of which show defects in glycosylation of secreted proteins and abnormal cell wall biosynthesis and assembly...
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Tài liệu Báo cáo khoa học: Mammalian Gup1, a homolog of Saccharomyces cerevisiae glycerol uptake/transporter 1, acts as a negative regulator for N-terminal palmitoylation of Sonic hedgehog doc
Ngày tải lên :
18/02/2014, 16:20
... These unique lipid modifications greatly reduce the diffusibility of Shh-Np and tether it to the cellular membrane However, they are necessary to regulate the movement of the protein to form the ... As the intensity of the fluorescence of these proteins was almost the same, the level of expression of these proteins was presumed to be simi322 lar To confirm this, we probed western blots of ... affecting the amount of Shh-N (Fig 1A–D, lane 5) 320 To examine in greater detail whether the effect of the expression of Skn on the 5E1 epitope of Shh-Np under denaturing conditions is a result of...
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Báo cáo khoa học: Energetic and metabolic transient response of Saccharomyces cerevisiae to benzoic acid docx
Ngày tải lên :
07/03/2014, 04:20
... ð12Þ time of the off-gas measurement, for which the contribution of the dilution in the fermentor headspace, the length of tubing connecting the fermentor with the off-gas analyzer and the response ... measured, the feasibility of this hypothesis could be studied based on data from the literature Indeed it has been reported that the cellular contents of these intracellular compounds of the same ... diameter is the diameter of the cell if the cell is assumed to be spherical b Roundness measures the shape of the object, it is defined as (perimeter2 · 1000) ⁄ (4 · p · area) The roundness of a circle...
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Báo cáo khoa học: Mutants of Saccharomyces cerevisiae deficient in acyl-CoA synthetases secrete fatty acids due to interrupted fatty acid recycling docx
Ngày tải lên :
07/03/2014, 05:20
... for the pool of bound fatty acids Therefore, the occurrence of 18:2 in the pool of free fatty acids would argue for lipid turnover as the source of fatty acid accumulation The expression of the ... state of the cells To gain further insight into the correlation of growth phase of the culture and the direction of fatty acid transport, we investigated the possibility of manipulating the transport ... contrast, the amount of free fatty acids in the medium of the cultures fed with raffinose increased by 100% within 30 h after adding the raffinose The supply of raffinose allowed the cells, on the other...
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Báo cáo khoa học: Lipins from plants are phosphatidate phosphatases that restore lipid synthesis in apah1Dmutant strain of Saccharomyces cerevisiae ppt
Ngày tải lên :
15/03/2014, 00:20
... recruited to the nucleus when the cells are cultivated in the presence of oleic acid A preliminary account of some of the results reported in the present study has been presented at the 19th International ... presumably as a result of the decreased ability of cells to utilize fatty acids for TAG synthesis [16] The PAH1 enzyme was found in soluble and membrane fractions of the cell and its association ... on the enzyme activity and resulted in the loss of up to 75% and 54% of the PAP activity of native enzyme, respectively (Fig 4) Interestingly, other mutations in the corresponding position of...
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Báo cáo khoa học: Schemes of flux control in a model of Saccharomyces cerevisiae glycolysis docx
Ngày tải lên :
17/03/2014, 11:20
... of possible (although inexact) fits to the data, accounts for the effect of simultaneously varying the kinetics of the other steps, and is also expected to be a better qualitative measure of the ... together, and form a different distribution to the experimentally determined values (B) Score explains over 99% of the total variance, so the gulf between the results of the original model and the ... providing a population of candidate fits that, if the fitness landscape of the model resembles that of the experimental system, may itself be considered to describe the population of the experimental...
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Báo cáo khoa học: ISC1-encoded inositol phosphosphingolipid phospholipase C is involved in Na+/Li+ halotolerance of Saccharomyces cerevisiae pptx
Ngày tải lên :
31/03/2014, 09:20
... Li+ ion extrusion from the cell We therefore investigated whether the loss of halotolerance in isc1D cells was due to the failure of ENA1 induction in the mutant For this, the ENA1-lacZ promoter–reporter ... proportion of isc1D cells had dropped to 2% of the viable cells, which compares to > 80% isc1D cells surviving in the absence of NaCl under otherwise identical conditions (Fig 2A) On solid media, the ... induction of the ENA1/PMR2 gene (designated as ENA1 in the following) encoding an enzyme of the P-type ATPase family This cation-extrusion pump promotes the efflux of Na+ and Li+ from the cell ENA1...
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Prediction of Saccharomyces cerevisiae replication origins pdf
Ngày tải lên :
09/08/2014, 20:20
... the about 2,000 candidates These histograms essentially describe the characteristics of the populations of known origins and of the set of candidates that passed the cutoffs described above The ... scaling the known histograms by a factor of 0.1 and subtracting them from the prediction histograms; the difference was then renormalized The value of 0.1 was chosen as an estimate of the proportion ... sensitivity of Oriscan predictions, where specificity is the fraction of predictions that match, and sensitivity is the fraction of the evaluation set that was predicted The specificity of the strongest...
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