load tax and hbz mrna expression anti htlv 1 antibody titers and neopterin concentration in cerebrospinal fluid
Retrovirology Research BioMed Central Open Access A novel HIV-1 restriction factor that is potx
... clone #1 10 clone #2 10 10 00 10 10 0 CEM.NKR 10 10 10 10 10 10 10 10 10 0 10 10 10 10 0 10 10 10 10 10 10 10 10 clone #3 10 clone #4 10 clone #5 10 0 .1 10 12 14 16 Days postinfection 10 10 B 10 10 000 ... 10 000 10 10 10 10 10 CEM-T4 10 10 10 10 10 0 10 10 clone #6 #8 10 0 10 0 CEM.NKR 10 00 10 10 10 clone #7 10 10 10 10 clone #8 10 #5 #7 10 #4 #1 10 10 10 #6 #3 #2 10 10 10 10 10 10 0 10 10 10 10 0 10 ... #1 clone #2 10 00 10 00 10 0 10 0 10 0 10 10 10 1 Actin 10 00 1 C 10 00 A3DE mRNA 0 .1 0 .1 10 12 14 0 .1 16 10 12 14 16 10 12 14 16 10 0 10 000 10 000 10 000 clone #3 clone #4 clone #5 10 00 10 00 10 00 10 0 10 0...
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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
... cell FEBS Journal 277 (2 010 ) 16 84 16 94 ª 2 010 The Authors Journal compilation ª 2 010 FEBS J Xiong et al 10 11 12 13 14 15 16 17 18 19 20 21 22 proliferation and adhesion-independent growth Cancer ... MDA-MB-2 31 cells were maintained in 10 % fetal bovine serum-supplemented L -15 (Gibco, Grand Island, NY, USA); SK-BR-3 cells were maintained in 10 % fetal bovine serum-supplemented RPMI -16 40 (Hyclone); ... the binding site of miR-22, forming pGL3m–ESR1–3¢UTR–MUT The ERa-expressing vector (pcDNA3 .1 ESR1) was created by cloning the ESR1 coding sequence into the EcoRI and NheI sites of pcDNA3 .1 (Invitrogen)...
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báo cáo hóa học:" Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF" ppt
... pM (matching probability) THO -1 D5S592 D10S526 vWA 31 D22S 417 FES/FPS 15 4 17 8 16 6–206 18 2–266 12 7 16 7 17 2– 213 206–234 0.77 0.83 0.84 0. 81 0.85 0.67 0.086; out of 12 0.0 51; out of 20 0. 017 ; out ... 2007, 11 7 :12 19 -12 22 Attinger CE, Janis JE, Steinberg J, Schwartz J, Al-Attar A, Couch K: Clinical approach to wounds: debridement and wound bed preparation including the use of dressings and wound-healing ... Definitions and guidelines for assessment of wounds and evaluation of healing Wound Repair Regen 19 94, 2 :16 5 -17 0 Brem H, Tomic-Canic M: Cellular and molecular basis of wound healing in diabetes J Clin Invest...
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Báo cáo sinh học: "Curcumin reduces expression of Bcl-2, leading to apoptosis in daunorubicin-insensitive CD34+ acute myeloid leukemia cell lines and primary sorted CD34+ acute myeloid leukemia cells" pptx
... Daunorubicin(0.2 g/ml) 0.09 1. 11 0 .11 0. 61 6.76 10 .58 4 .12 22.63 97.55 1. 25 97.90 1. 38 76. 31 6.35 60.82 12 .43 11 .86 5.73 9 .19 9.74 42.06 12 .62 86.35 81. 28 1. 13 1. 60 45.76 2.44 0.74 0.29 18 .77 Kasumi -1 ... http://www.translational-medicine.com/content/9 /1/ 71 A Page of 15 KG1a Kasumi -1 U937 96. 71 99.72 M1 0 .18 M1 M1 Cell viability(%;MTT assay) B *** *** *** Daunorubicin( g/ml) C Daunorubicin( g/ml) 0.4 0.8 1. 6 1. 65 10 .15 ... competing interests Received: November 2 010 Accepted: 19 May 2 011 Published: 19 May 2 011 Rao et al Journal of Translational Medicine 2 011 , 9: 71 http://www.translational-medicine.com/content/9 /1/ 71...
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báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... and was assisted in these experiments by JAB and JAAG MM and CR helped in designing appropriate Muc16 primers JC assisted in obtaining and maintaining murine ovarian tumor cells MK assisted in ... and intracellular Muc16 in the four MOVCAR cell lines was determined by flow cytometry using the 618 F antibody The mean fluorescence intensity for the binding of 618 F to the cell surface and intracellular ... line (B) MOVCAR -10 cells were fixed and expression of intracellular Muc16 was detected by using the 618 F antibody (grey line) Dark line shows isotype control (C) Expression of cell surface and...
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Báo cáo y học: " Inhibition of constitutively active Jak-Stat pathway suppresses cell growth of human T-cell leukemia virus type 1-infected T-cell lines and primary adult T-cell leukemia cells" potx
... HTLV- 1infected T-cell lines and primary ATL cells [12 ,14 ] Expression of Stat5 mRNA is induced by HTLV- 1 Tax using JPX9 cells [32] Using this cell line, we showed that Tax induced neither the expression ... Signaling Technology (Beverly, MA) The anti- phospho-Jak1 (Tyr 10 22/Tyr 10 23), antiphospho-Jak2 (Tyr 10 07/Tyr 10 08), anti- phospho-Jak3 (Try980), anti- cyclin D2, anti- Pim -1, anti- survivin and anti- c-IAP2 ... HTLV- 1- negative [HTLV- 1 (-)] and three HTLV- 1- infected [HTLV- 1 (+)] T-cell lines The blots were probed with anti- phospho-Stat3, anti- Stat3, antiphospho-Stat5, anti- Stat5 and anti -Tax Amounts of actin are shown...
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Evaluation of thymoquinone for cytotoxic activity against human breast cancer cell lines and tumor xenograft in nude mice
... Fostira, 2 013 ) In addition to BRCA1/2, TP53 and PTEN, there are other high-penetrant genes such as STK 11 (serine/threonine kinase 11 ) and CDH1 (cadherin 1) Mutation of STK 11 gene increased the ... signal-regulated kinases 1/ 2 (ERK1/2), c-Jun amino (N)-terminal kinases 1/ 2/3 (JNK1/2/3) and p38 isoforms (α, β, γ and δ) Usually, ERK1/2 is involved in cell proliferation and differentiation, while JNK and ... 38 2 .13 Gene silencing with siRNA 39 2 .14 Breast tumor xenograft mouse model 39 2 .15 Hematoxylin and Eosin (H&E) staining 40 2 .16 TUNEL staining 41 2 .17 Ki67 immunohistochemistry...
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Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
... recombinant full-length Cdt1 and geminin (HisT7-geminin and His-geminin, amount of recombinant protein run in each lane in ng is indicated) and immunoblotted with anti- Cdt1 and anti- geminin Ig ... inhibiting its MCM loading function [12 ,15 ,16 ] During the cell cycle, geminin is expressed in S and G2 phases, when licensing should be inhibited and when the Cdt1 protein is undetectable [13 ,16 ] ... and geminin mRNAs are down-regulated in G0 During the cell cycle, Cdt1 and geminin mRNA levels are mostly stable and protein levels are primary controlled by Fig Expression of Cdt1 and geminin proteins...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
... 10 4 10 1 10 2 FL1-H 10 3 10 4 60 40 Counts 20 80 10 0 60 40 20 0 10 0 10 2 FL1-H 10 0 Counts 60 40 20 Counts Lung 10 1 80 10 0 10 0 80 10 0 10 0 60 80 10 0 20 0 Counts 10 2 FL1-H Muscle 10 1 30 60 90 12 0 15 0 18 0 ... could indicate that mtDNA-depleted cells need less Homeostasis of ROS in q0 cells (Eur J Biochem 2 71) 3653 Ó FEBS 2004 ρ+ 10 3 10 4 10 1 10 2 FL1-H 10 3 10 4 10 1 10 2 10 3 10 4 10 3 10 4 10 1 10 2 FL1-H 10 3 10 4 ... medium containing 4.5 gÆL )1 glucose, 11 0 mgÆL )1 pyruvate, supplemented with 10 % (v/v) fetal bovine serum, 10 0 unitsÆmL )1 penicillin, and 0 .1 mgÆmL )1 streptomycin, at 37 °C in a humidified atmosphere...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
... HLA-DR CD 71 CD 71 CD 71 CD 71 12.6 2.3 31. 2 16 .3 13 .6 14 .1 2 .1 1.9 + MbCD + MbCD + MbCD + MbCD ± ± ± ± ± ± ± ± 1. 9 1. 5 0.9 1. 1 2.2 2.6 0.8 1. 1 In order to see whether localization of CD25 in DRM depends ... containing 73% (w/v) sucrose and lL of protease inhibitor cocktail (1. 5 mgÆmL )1 aprotinin, 1. 5 mgÆmL )1 leupeptin, 1. 5 mgÆmL )1 pepstatin, 70 mM benzamidin, 14 mM diisopropyl fluorophosphate and ... virus I (HTLV- I) [24] MT -1 is also an adult T cell leukemia cell line associated with HTLV- 1 and is deficient in the signaling IL-2Rb and c subunits [25] All cell lines were cultured in RPMI -16 40...
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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx
... 1. 15 .1. 1), glutathione peroxidase (GPX, EC 1. 11. 1.9) and catalase (EC 1. 11. 1.6), and low molecular weight antioxidants, such as vitamins and various biological dyes, can reduce ROS in vivo [16 19 ] ... H2O2 and lipid hydroperoxides [10 12 ] In addition, ROS derived from leukocytes induce excessive in ammation, leading to cell and tissue damage [13 ,14 ] In extreme instances, such as endotoxin shock, ... show antioxidant, antinociceptive, neuroprotective and anti- in ammatory properties in different experimental models [30–35] Ebselen inhibits leukocyte in ltration and activation, leading to elimination...
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Báo cáo khoa học: Fast set-up of doxycycline-inducible protein expression in human cell lines with a single plasmid based on Epstein– Barr virus replication and the simple tetracycline repressor ppt
... fluorescence intensity was 19 5 in the off-state and 18 60 in 786 Doxycycline – Relative background transcription (%) 0. 014 0. 014 0.0 011 0.0052 0.045 0.0 017 0.068 0.0077 0.0 31 0. 012 1. 4 1. 6 0.096 0.33 1. 9 ... X -10 0 in mmolÆL )1 Tris-HCl pH 7.4, and radioactivity was determined by liquid scintillation counting Uptake of ergothioneine (10 lmolÆL )1) was determined by LC-ESI-MS ⁄ MS Cells were assayed and ... Biol 21, 414 9– 416 1 Yates JL, Warren N & Sugden B (19 85) Stable replication of plasmids derived from Epstein–Barr virus in various mammalian cells Nature 313 , 812 – 815 Lupton S & Levine AJ (19 85)...
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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf
... 445:8 51- 857 Niehr et al Journal of Translational Medicine 2 011 , 9:76 http://www.translational-medicine.com/content/9 /1/ 76 10 11 12 13 14 15 16 17 18 19 20 21 Flaherty KT, Puzanov I, Kim KB, Ribas A, McArthur ... combined in constant ratios to each other Among the 11 BRAFV600E mutant cell lines, in cell lines the combination was antagonistic and in cell lines it was synergistic (Figure 1b) In two cell lines ... described [19 ] Primary antibodies included pCRAF (Ser289/296/ 3 01) , CRAF, pMEK1/2 (Ser 217 /2 21) , MEK1/2, pERK1/2 (Thr202/Tyr204, Thr185/Tyr187), ERK1/2, pLKB1 (Ser428), LKB1, pAMPKa (Thr172), AMPKa, pACC...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
... SK-NEP -1 0.00 1. 82 HT 10 80 19 .37 5.92 SA-4 12 .53 1. 05 ** SW 19 61 64.55 3.04 HS 919 3. 01 5. 31 KATO-III 7.65 2 .16 SW 80 -5.00 3.93 -56.35 1. 45 ** SW 732 ** DLD -1 69 .19 65.59 1. 29 3 .19 HS 913 T Lung ... 0.83 11 .53 Thyroid (1/ 2) Brain (2/9) Breast (0/9) ** NU-04 69. 41 4.02 SKBR3 -0.09 13 .53 ** U 118 MG SW 598 51. 96 14 .29 1. 48 4 .14 MDA-MB-468 GI -10 1 -3.46 -6. 41 0.25 0.92 A172 2.26 4.97 SV-BR -1 -8.00 ... inhibitors of ARG -1 (nor-NOHA), iNOS (L-NMMA), NOX2 (apocynin), VEGF (neutralizing antibody Avastin), or TGFb1 (SB4 315 42 or neutralizing antibody 1D 11) In these studies no one inhibitor was found to completely...
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Báo cáo sinh học: " High Chromosome Number in hematological cancer cell lines is a Negative Predictor of Response to the inhibition of Aurora B and C by GSK1070916" potx
... Medicine 2 011 , 9 :11 0 http://www.translational-medicine.com/content/9 /1/ 110 cancer targets for therapeutics and multiple Aurora kinase inhibitors are currently being studied in early phase I and ... GSK1070 916 in malignancies such as NHL A number of Aurora kinase inhibitors are already in clinical or preclinical development including GSK1070 916 , VX-680, AZD 115 2, PHA-739358, AT9283 and CYC 116 [24-28] ... Spiess PE, Steinberg JR, Wang Z, Katz RL, et al: Quantitation of Aurora kinase A gene copy number in urine sediments and bladder cancer detection J Natl Cancer Inst 2008, 10 0 :14 01- 1 411 11 Staff S,...
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Báo cáo hóa học: " Quantitative profiling of housekeeping and Epstein-Barr virus gene transcription in Burkitt lymphoma cell lines using an oligonucleotide microarray" docx
... 0. 01 0 .12 0. 51 1.46 2.03 1. 26 2.08 1. 92 2.08 1. 00 0.25 0.20 0.00 0.32 0. 01 0.09 0. 01 0 .18 0.49 0 .14 0.00 0.25 0. 01 0.05 0.00 0 .18 0.75 1. 04 1. 35 1. 18 1. 35 1. 35 1. 35 1. 29 0 .10 0 .15 0.04 0 .11 0.04 ... 2.45 0.62 0. 01 0.67 0.62 0. 01 0.76 0.76 0.77 0. 01 0.05 0.28 0.27 0.02 0. 31 0. 31 0. 31 0. 01 0 .10 1. 08 1. 61 1.43 1. 64 1. 51 1.62 0.59 0.05 0.05 0 .12 0 .13 0 .13 0.07 0 .12 0.04 0.54 0.04 0.08 0.09 0.08 ... CS 11 21 416 82.4 60.0 CS 70 16 76 903 82.4 60.0 CS Hs .11 8786 70 19 29 15 4 78.8 51. 4 CS 384D8-2.2 Hs.356523 71 22 81 334 80.6 52.2 CS Hs .11 8786 70 19 29 15 4 78.8 51. 4 CS PSMD2 Hs.74 619 70 2828 12 2...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến dòng điện stato i1 fi p2 sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25