... meanings, grammar, and pragmatic or local context, receive support as separately definable knowledge within studies of aphasia There is a vast l i t e r a t u r e concerning what aspects of language ... neurolinguistic l i t e r a t u r e demonstrates that the grammar can be affected in isolation from other aspects of language function (Cf Studies of agrammatic and Broca's aphasia as described in Goodenough, ... syntactic category aspect of each meaning of a phonetic word is also a part of the grammatical representation where i t makes associations with other syntactic categories The associations...
... Honda T, Sasakawa C, Ogasawara N, Yasunaga T, Kuhara S, Shiba T, Hattori M, Shinagawa H: Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory ... data, and wrote the manuscript Additional data files The following additional data are available with the online version of this paper Additional data file is a figure showing the overall mean ... revealed by whole genome PCR scanning Proc Natl Acad Sci USA 2002, 99:17043-17048 Ogura Y, Ooka T, Asadulghani , Terajima J, Nougayrède JP, Kurokawa K, Tashiro K, Tobe T, Nakayama K, Kuhara S,...
... course data with a two-exponential association model kobs(rapid) and kobs(slow) are the observed association rate constants for the rapid and slow association phases, respectively The values for ... plateau)*exp() KX) + plateau or the two-phase exponential decay equation Y = plateau + SpanFast*exp() KFastX) + SpanSlow*exp() KSlowX) The data were also analyzed by plotting dissociation data ... internalization and whether the rate constants of association and dissociation for [125I]TCPCSK9 at 37 °C are similar to values obtained at °C Our preliminary data indicate that HepG2 cells are able...
... using BSA asa standard Data analysis Data are expressed as mean ± SEM from three or more separate experiments with triplicate samples One-way or two-way analysis of variance (anova) was used ... supported by grants from the Diabetes Australia Research Trust and the Australian Research Council B E Brown and I Rashid gratefully acknowledge receipt of Australian Postgraduate Awards administered ... cell medium samples were collected, and the cells were washed and lysed in water Cell viability was determined by assaying lactate dehydrogenase release, and cellular cholesterol and cholesteryl...
... capsaicin and their combination on the damage caused to liver by iron overloading measured in terms of lipid peroxidation and elevation of plasma alanine aminotransferase (AlAT), aspartate aminotransferase ... be assessed by leakage of enzymes such as alanine aminotransferase (AlAT), aspartate aminotransferase (AsAT) and lactate dehydrogenase into blood [52,53] Higher activities of all these three enzymes ... described by Yagi [27], using 1,1,3,3-tetraethoxypropane as reference Serum enzymes Plasma-nonspecific enzymes, aspartate aminotransaminase (AsAT, EC.2.6.1.1) and alanine aminotransaminase (AlAT, EC.2.6.1.2),...
... classical shape characterized by a lag time followed by a linear increase until a maximum is reached Then they slowly decay [45] When separately added, 10 lM urate decreases the lag-time whereas ... Polyphenolic flavanols as scavengers of aqueous phase radicals andas chain-breaking antioxidants Arch Biochem Biophys 332, 339–346 19 Tikkanen, M., Wahala, K., Ojala, S., Vihma, V & Adlercreutz, ... investigation appears sensible as it was recently demonstrated [36] that, at low concentration, urate may behave asa pro-oxidant Moreover, in an earlier report it was suggested that flavonoids and urate could...
... acylglycerols, assuming 1-random, 2-random and 3-random distribution [27] The molecular associations give the exact pairs of fatty acids in individual diacylglycerol and the exact triplets of fatty acids ... were prepared after partial deacylation of plasma VLDL triacylglycerols and they were separated and analysed by HPLC/MS as described in Methods Results are expressed as mean ± SD for five rats per ... hydrolysis of 20:4 containing triacylglycerol and diacylglycerol was slower in rat postheparin plasma when hepatic lipase was inhibited [36] Hepatic lipase is capable of hydrolysing fatty acids from the...
... preparations were rst incubated with Cu2+ and then urate was added after LPO started As already shown in Fig 1, urate at high concentration added before Cu2+ behaves as an antioxidant In contrast, ... increasing or decreasing phases, because different LDL preparations we used to get data at least in triplicates As to the MDA formation, Fig 2A and C fully conrm the pro-oxidant activity of low ... Free Radic Biol Med 22, 411421 Nyssonen, K., Porkkala-Sarataho, E., Kaikkonen, J & Salonen, J.T (1997) Ascorbate and urate are the strongest determinants of plasma antioxidative capacity and serum...
... phase, and plasma lipoprotein phospholipase A2 activity as defined from univariate analysis after adjustment for age and female gender Page of (page number not for citation purposes) Available ... Correlation between HDL-associated lipoprotein- associated phospholipase A2 (HDL-Lp-PLA2) activity and autoantibody titers against oxLDLD in early rheumatoid arthritis patients at baseline Page ... Lp-PLA2 activities and elevated autoantibody titers against mildly oxLDL The low plasma Lp-PLA2 activity and the increased titers against oxLDLD are independently associated with ERA, suggesting an...
... microglial cells indicated that the endolytic degradation of A was dramatically enhanced by apoE3 rather than apoE4 [69] Moreover, apoE4 potentiated A -induced lysosomal leakage and apoptosis, leading ... β-amyloid BLAST Basic Local Alignment Search Tool BSA bovine serum albumin C83 C-terminal fragment 83 C99 C-terminal fragment 99 CIP Calf Intestinal Alkaline Phosphatase CK1 casein kinase CNS central ... intracellular domain The image was taken from Ref [38] 1.2.2 Late-onset AD Over 99% of AD cases occur late in life (>65 years) and are referred as late-onset AD (LOAD) Genome-wide association...
... which are Chlamydia pneumoniae, lowdensitylipoproteinand macrophage; and not due to Chlamydia pneumoniae alone In the fourth model, lowdensitylipoprotein was added to the macrophages and allowed ... the bacteria Chlamydia pneumoniae, is added to the macrophages first and allowed to interact with the macrophages for 72 hours; and then after which lowdensitylipoprotein is added and allowed ... interact with the macrophages for 24 hours; and then after which the bacteria Chlamydia pneumoniae, is added, and allowed to interact with both the macrophages andlowdensitylipoprotein for another...
... of an antioxidant molecule, AH, either the initiating peroxyl radical and ⁄ or the propagating lipid peroxyl radical can be trapped anda • • new antioxidant radical, A , produced If the A is a ... diseases, such as rheumatoid arthritis, ischaemia–reperfusion injury to heart muscle and Alzheimer’s disease [42] Protein carbonyl formation is a biomarker of protein oxidation and has some advantages ... 0.5% agarose gels and stained with Sudan Black B The REM was defined as the ratio of migrating distance of oxidized LDL to that of native LDL Statistical analysis Results are expressed as mean ±...
... acetic acid, pH 2.6, and 0.1 m NaCl The remaining radioactivity was dened as internalized ligand Internalized and cell surface-associated ligand were expressed asa percentage of the total radioactivity ... the same way as uPAPAI-1 except that an anti-uPA mAb2-coupled Sepharose column was used instead of an anti-uPA mAb6coupled Sepharose column To prepare nonradioactive wild-type and mutant uPA PAI-1 ... model was poor As appears from Fig 5, the rapid rst phase of the association was followed by a second, slower phase, anda corresponding rapid dissociation phase, amounting to 20% of total binding,...
... 5′-TTTggatccACTGATGAGACAGAGGAGCACTGT-3′ and 5′-TTTgtcgacATCAACAGCTTGGATATATTCCTCATC-3′ for cDNAF6 (N11826–12374), 5′-TTTgaattcCGAAAATATAATCTCTCATCT-3′ and 5′-TTTgtcgacCTGTTTGCAAAGGTTGGGCACTGA-3′ ... 5′-TTTgtcgacAAAAATGAGATAGGGTTCGATGTTA-3′ for cDNAF2 (N9294–9713), 5′-TTTgaattcAACAGTAACATCGAACCCTATCTC-3′ and 5′-TTTgtcgacATTGTTGGTACCACAGGGATTGC-3′ for cDNAF3 (N9684–10523), 5′-TTTggatccAATCCCTGTGGTACCAACAATGGT-3′ ... 5′-TTTggatccAATCCCTGTGGTACCAACAATGGT-3′ and 5′-TTTgtcgacACAGCCTTGCTCATCACTGTTGTC-3′ for cDNAF4 (N10494–11180), 5′-TTTgaattcGAATTCAGCTGCAAAACAAATTAC-3′ and 5′-TTTgtcgacCTCTGTCTCATCAGTTCCATCTCC-3′ for cDNAF5 (N11157–11855),...
... 5’-CGGGATCCAAGAATCAAAGAGGGAACTGAA-3’ (5’-end) and 5’-CCGCTCGAGACCTGAAGAGTTTGCAGCTCT-3’ (3’-end), which introduced BamHI and XhoI restriction sites (underlined) The BamHI/XhoI-fragment was then ... investigations of values in multifactorial design were examined by means of two-way analysis of variance (two-way repeated-measures ANOVA) A value of P < 0.05 was considered statistically significant ... Western blot, and RT-PCR analysis SW and CT carried out intravital microscopy ML and CL conceived of the study, analyzed data, and drafted the manuscript DP, MM, and VC made substantial contributions...
... experimentally obtained relaxation rates R1 (longitudinal or spin–lattice relaxation rate) and R2 (transverse or spin–spin relaxation rate) and NOE values for the amide 15N nuclei measured at 278 K, and ... ª 2007 The Authors Journal compilation ª 2007 FEBS 4233 NMR structureanddynamics of eRF1 middle domain E V Ivanova et al saturation was applied asa relaxation delay for NOE enhancement in ... relaxation data Protein Sci 9, 1210–1216 71 Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W & Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database...
... excision repair J Biol Chem 278, 7476–7485 Masutani C, Araki M, Sugasawa K, van der Spek PJ, Yamada A, Uchida A, Maekawa T, Bootsma D, Hoeijmakers JH & Hanaoka F (1997) Identification and char- FEBS ... Solvent-accessible surface areas of XPCB–hHR2 3A and B The solvent-accessible surface areas and their deviations for each residue are shown for two XPCB–hHR2 3A structures; Waters et al [13] (A1 ), Kamionka and Feigon ... the ATPase domain of Hsp70-like Stch FEBS Lett 467, 348–355 Araki M, Masutani C, Takemura M, Uchida A, Sugasawa K, Kondoh J, Ohkuma Y & Hanaoka F (2001) Centrosome protein centrin ⁄ caltractin...
... b-1,4-galactosyltransferase was from Calbiochem b-galactosidase (bovine brain) and b-N-acetylglucosaminidase (Jack Bean) were from Sigma Metabolic radiolabelling of M25 Zaire P falciparum parasites Asexual blood stage ... immunoprecipitated 19 kDa protein was detectable only asa single 19-kDa band The only strong [3H]GlcN-labelled band in the total extract matching the Western blotted material was a 17-kDa band The majority ... P04933) were aligned according to [23] and designated as G (Ghana), P (Png MAD20), U (Uganda), T (Thai) and W (Wellcome) Alpha- and b-GlcNAc site predictions, made using methods based on neural networks,...
... CGCGCTAGCATGATCAAGGACTGTGGG CAGCCC and CGCGAATTCTCACACTGGCAAGC ACCGAGGAATCT; CCP1CCP2: CGCGCTAGCATGATCATCAAGTGCC CCCAGCCC and CGCGAATTCTCACACTGGCAA GCACCGAGGAATCT; CUB2CCP1: CGCGCTAGCATGACTCAGGCTGAG ... CGCGCTAGCATGACTCAGGCTGAG TGCAGCAGC and CGCGAATTCTCAGTCCTTGA TCTTGCATCTGGG; CUB2CCP1CCP2: CGCGCTAGCATGACTCAGGCT GAGTGCAGCAGC and CGCGAATTCTCACACT GGCAAGCACCGAGGAATCT The PCR products were digested with NheI and EcoRI ... (R2) relaxation, as well as {1H}-15N NOE measurements, were made at 11.7 T These relaxation data were acquired and analyzed for both CCPsingle modules at pH 4.0–4.5 and pH 7.0 at 300K (Table S1)...
... NMR structureand backbone dynamics of MC1 F Paquet et al HTa, a member of the HU family [5,6] Sac7d and Cren7 have a small b-barrel with or without an amphiphilic C-terminal a- helix, and HUs have ... 15N-relaxation parameters (R1, R2, and NOE) for MC1 NMR relaxation experiments were measured at 299 K on a Varian 500 MHz (NOE), Varian INOVA 600 MHz (NOE, R1 and R2) and Varian INOVA 800 MHz (R1 and ... that have enabled us to assign all side chains and to introduce dihedral angle restraints (u and w angles) Residual 5134 dipolar couplings (RDCs) were also measured in a partially aligned sample...