life as long as that of the animal
Cell Cycle Regulation in Mammalian Germ Cells
... called the M-phase-promoting factor (MPF) (Choi et al 1991; Masui and Markert 1971) The build up of cyclin B and its degradation at the end of the cell cycle is a hallmark of MPF activity guiding the ... degradation by a phosphatase KAP The Cdc25 family of phosphatases are activators of Cdk complexes by removal of the phosphates from the ATP-binding site of Cdk1, but their function may differ ... mutants, the number of foci increase, consistent with the proposed role of RAD51 for binding along the DSB and performing the DNA strand invasion search during homologous recombination Due to the...
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scientific american - 1996 10 - hell's cells
... international the fisheries Although the Ministry of waters off the southern coast One ma- Food Processing Industries has issued jor concern of the NFF is that the licens- the licenses, the Commerce ... But whether the increase was the result exclusively of radiation from Chornobyl or of other agents as well is still a matter of discussion In any event, until the end of 1995, a total of 682 ... evidence that the canted grain of the inner core was indeed swiveling around relative to the rest of the earth Their idea was to examine seismic recordings of waves that traveled through the inner...
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báo cáo hóa học:" Aurora kinase inhibitors synergize with paclitaxel to induce apoptosis in ovarian cancer cells" pot
... treatment, the Caspase- Glo™ 3/7 reagent was added in a 1:1 volume ratio to each well of the 96 well luminometer plate Immediately following the addition of the reagent, the contents of the wells ... treated with increasing doses of VE-465 demonstrate dosedependent increase in apoptosis The caspase activity was blocked by the pan-caspase inhibitor Z-VAD with VE-465 bypass the spindle checkpoint ... death were only slightly increased when treated with VE-465 in the presence of paclitaxel (Fig 4C and 4D) Caspase 3/7 assays of PTX10 cells confirmed that there was no statistically significant...
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báo cáo hóa học:" KSP inhibitor ARRY-520 as a substitute for Paclitaxel in Type I ovarian cancer cells" potx
... increase in the activity of caspases- 8, 9, and was observed in a time-dependent manner (Fig 2a), with a corresponding decrease in the levels of XIAP (Fig 2b) Moreover, we saw the appearance of the p30 ... showed that ARRY-520 is able to induce caspase-2 activation in a timedependent manner similar to that observed with the other caspases-9, -8, and -3 (Fig 2b) Previous studies showed that caspase-2 ... represents non-treated controls (a) Activity of capase-3, -8, and -9 was measured using Caspase-Glo assay, and (b) effect on XIAP, Caspase-2, and Bid was determined using Western blot analysis Results...
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báo cáo hóa học:" ST6Gal-I expression in ovarian cancer cells promotes an invasive phenotype by altering integrin glycosylation and function" pptx
... altering the function of the β1 integrin receptor Discussion Peritoneal metastasis of epithelial ovarian carcinoma is the primary means of metastatic spread, although a small minority of tumors ... through the Matrigel to the underside of the filter were quantified As shown in Fig 5B, the ST6 cells were more invasive than either P (p < 0.05), or EV (p < 0.05) No difference was observed in the ... not predictive of outcome [16,17] The role of ST6Gal-I in ovarian carcinoma has not been as clearly defined as its effect in some other tumors, namely colon and breast Nonetheless, there are recent...
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báo cáo hóa học:" Sensitization of ovarian cancer cells to cisplatin by genistein: the role of NF-kappaB" doc
... effects on breast [13], pancreas [27,29], and melanoma cells [30], associated with the inhibition in the translocation of p65 subunit of NFκB in the nucleus, which is otherwise increased by the cisplatin ... inactivation of NF-κB signaling, resulting in the inhibition of cell proliferation and induction of apoptosis (Figure 5) Our hypothesis is also supported by the evidence of down-regulation of the important ... sensitize even the drug-resistant cell line and cause inhibition of cell viability Further, to assess whether the loss of overall cell viability could also be due to the induction of apoptotic...
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báo cáo hóa học:" Trastuzumab Sensitizes Ovarian Cancer Cells to EGFR-targeted Therapeutics" docx
... inhibitor [35] Based on these results, we and others have proposed that comprehensive analysis of expression of all four HER family members, and their isoforms, as well as key components of their signaling ... proliferation of all four tested cell lines, whereas gefitinib inhibited the proliferation of A1847 and SKOV-3 cells, and cetuximab inhibited the proliferation of OVCAR-7 and SKOV-3 Furthermore, subsets of ... Even in the well studied case of breast cancer, Paik et al., have shown that patients with tumors expressing even low levels of HER2 may gain benefit from trastuzumab therapy [33] Together, these...
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báo cáo hóa học:" Curcumin induces chemo/radio-sensitization in ovarian cancer cells and curcumin nanoparticles inhibit ovarian cancer cell growth" pot
... level of full-length caspase and was decreased, suggesting cleavage and activation of the caspase pathway; however, cleaved products of caspase or were not detectable (data not shown) Furthermore, ... medium was used as a control The anti-proliferative effect of these drugs was determined at days with a MTS based colorimetric assay (CellTiter 96 AQeous One Solution Cell Proliferation Assay, ... by cisplatin increased the percent of Annexin V positive cells and increased the amount of cleaved caspase and PARP, as compared to cisplatin or curcumin alone, indicating that curcumin pre-treatment...
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báo cáo hóa học:" Anti-proliferative effect of LXR agonist T0901317 in ovarian carcinoma cells" pptx
... 72 hours of treatment, the LXR agonist T0901317 decreased the percentage of cells in S phase and increased the percentage of cells in the G0/G1 phase, indicating a cell cycle arrest at the G1-S ... activation of caspase was confirmed by Western Blot analysis LXR agonist treatment enhances caspase activation, resulting in increased caspase precursor cleavage rate and decreased caspase precursor ... cells by measurement of caspase -3 and -7 activity via flow cytometric analysis Figure 4A shows the percentage of cells in early apoptosis, as assessed by Vybrant FAM Caspase 3-and Assay Kit and...
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báo cáo hóa học:" MUC4 stabilizes HER2 expression and maintains the cancer stem cell population in ovarian cancer cells" ppt
... of MUC4 in altered growth behavioral properties of the tumor cell [8] The ectopic expressed MUC4 gene has all the basic elements of the MUC4 gene but its tandem repeat region is only 10% of the ... the breast cancer (MCF7) cell line [11] The fact that the vast majority of MCF7 stem cell enriched SP cells express MUC1 suggests that epithelial cancer stem cells would also be the targets of ... cancer is the fourth leading cause of cancer deaths among all women and has the highest mortality among the gynecologic cancers It is one of the most challenging of all cancers to fight as it often...
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báo cáo hóa học:" Gene Expression and Pathway Analysis of Ovarian Cancer Cells Selected for Resistance to Cisplatin, Paclitaxel, or Doxorubicin" docx
... Because of the specific proteasome genes found altered, as well as the presence of cell cycle genes differentially expressed (such as CDK7), it is likely that the proteasome pathway changes affect the ... mediate the development of drug resistance and include 1) changes in the regulation or repair of the primary target of the drug (DNA, microtubule, etc), 2) drug retention (increased influx or decreased ... compared the drug resistance mechanisms associated with the development of resistance to different drugs 10 We found that the gene expression changes associated with the development of drug resistance...
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Báo cáo sinh học: "TBP2 is a general transcription factor specialized for female germ cells" doc
... possibility would be that the amino-terminal domain of TBP2 could determine the association of TBP2 with a special set of TAFs and/or other oocyte-specific factors that, in turn, would confer the oocyte-specific ... remains: why is there a distinct requirement for either of the two TBP paralogs in oocytes and embryos? The high level of divergence of the amino termini between TBP2 and TBP may hold the key to this ... address the function of TBP2, in their recent BMC Biology paper Akhtar and Veenstra [5] have investigated the role of TBP2 in oocyte transcription and compared it with that of TBP They show that...
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Báo cáo y học: "On the number of founding germ cells in humans" ppt
... here and defined in the model For the sake of clarity, the age coordinate is expanded artificially during the embryonic phase The proliferative phase initiates at the time of germline-soma separation ... first half of fetal development and then begin a progressive decline that extends throughout the reproductive years The initial phase initiates with the separation of the germline from the soma, ... collected, a fuller discussion of the modeling issues is deferred here ently of one another In addition, the model depends crucially on the concept that the cessation of proliferation and entry into...
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Báo cáo y học: "Enigma variations: control of sexual fate in nematode germ cells" docx
... maintain the ability to produce sperm The FEM proteins are therefore active in the germlines of both sexes in C briggsae But where in the developmental pathway? And how? If the role of the fem ... in life And although tra-2 mutants normally produce only sperm, the addition of a fem mutation caused these animals to switch to oogenesis later in life, suggesting that in hermaphrodites too the ... suggested that the entire sex-determination pathway might be the same in these nematodes, so Nayak et al [22] used the C briggsae genome sequence to search for the other factors And this is where the...
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Interactions between early germ cells and inner germarial sheath cells in drosphila ovary
... enter the nuleus of GSCs The entry induces activation of target genes, such as dad, and in the meantime repression of bam in GSCs 17 Figure 1.3 The canonical model of JAK-STAT signaling Binding of ... activation of the receptor-associated JAK JAK then phosphorylates itself and the receptor to generate docking sites for the SH2 domains of STAT STAT are normally present in the cytoplasm as inactive ... believed that the GSC niche together with the escort cells, produces extrinsic factors to regulate the behaviors of the stem cells and their progenies 1.2 GSC Niche Activities in Drosophila Ovary The...
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Tài liệu Báo cáo khoa học: Expression and secretion of interleukin-1b, tumour necrosis factor-a and interleukin-10 by hypoxia- and serum-deprivation-stimulated mesenchymal stem cells Implications for their paracrine roles ppt
... and that processing of pro-IL-1b into mature IL-1b requires activating cleavage of pro-caspase [23], the cleavage of both pro-IL-1b and pro-caspase was examined in hypoxia ⁄ SD-stimulated MSCs As ... signal, such as the endotoxin LPS, promotes the synthesis and cytoplasmic accumulation of pro-IL-1b A second signal, in the form of exogenous ATP, triggers caspase 1-mediated processing of pro-IL1b ... work demonstrates that hypoxia ⁄ SD increases the transcription but not the secretion of IL-1b and TNF-a, suggesting that the roles of these factors in the paracrine effects of MSCs are negligible...
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Báo cáo Y học: A new siglec family member, siglec-10, is expressed in cells of the immune system and has signaling properties similar to CD33 docx
... the Kinase assay results The kinase assays indicate that the cytoplasmic domain of the siglec protein can be phosphorylated by representatives of at least three of four major families of kinases: ... was cloned into pd19 by digesting the fragment with HindIII and Bgl II and the plasmid with HindIII and Bam HI The fragment was ligated into the plasmid and the integrity of the insertion was ... necessary for the recruitment of SHP-1 and partial recruitment of SHP-2 The q FEBS 2001 recruitment of SHPs suggests that the function of siglec-10 is similar to that of other siglecs such as siglec-3/CD33...
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Báo cáo hóa học: "Inhibiting adenoid cystic carcinoma cells growth and metastasis by blocking the expression of ADAM 10 using RNA interference" pdf
... limited The purpose of this study was to analyze the relationship between the gene silencing of ADAM 10 and the invasive and metastatic potentials as well as the proliferation capability of adenoid ... IV of the basement membrane and is relevant to tumor metastasis [8] In another study, it was shown that the cleavage of CD44 catalyzed by ADAM 10 contributed to the migration and invasion of ... conditions All animal experiments were carried out according to the standards of animal care as outlined in the Guide for the Care and Use of Experimental Animals of the Medical College of Shanghai...
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Solar Cells Dye Sensitized Devices Part 10 potx
... proportional to the lifetime of the photoelectron in the DSSCs, and the lifetime of the electron is inversely proportional to the rate of recombination.[40] The HNO3-HF treatment of the Ti substrates ... structure with the nano-crystalline TiO2 layer The almost same electrochemical impedance of the W with the Ti was also reported Under the assumption that most of the oxide layer is WO3, the conduction ... Furthermore, the size of the 2nd semicircle (low frequency range) was also largely decreased The 2nd semicircle is related to the recombination of electrons with I3.[14] Under the assumption that...
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Solar Cells New Aspects and Solutions Part 10 pptx
... In the same study, although the light emitting intensity in the ITO/GaN LED was enhanced compared with that of a Ni/Au/GaN LED under the same current density, the lifetime of the device was significantly ... on the frequency of measurement Figure shows the capacitancefrequency (C–f) characteristics of the samples The characteristics were measured under zero-bias conditions As seen in the graph, the ... extrapolated fit-line of the plots Figure 4(b) shows the plot of 1/CD2 as a function of the applied voltage The frequencies used for the capacitance measurements were 100 Hz and KHz for the PANI/epi.-GaN...
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