isolation of stem cells from normal and tumor breast tissues

Fibroblast-like synovial cells from normal and inflamed knee joints differently affect the expression of pain-related receptors in sensory neurones: a co-culture study ppsx

... supernatants of cultured DRG neurones, cultured FLS cells and co-cultures of DRG neurones and FLS cells (from normal and acutely or chronically inflamed joints) were analysed for the production of prostaglandin ... Co-culture system As a first step we characterized the morphology of FLS cells and DRG neurones in the co-culture system The FLS cells formed a flat layer composed mainly of triangular NSE-negative cells ... neurones Morphology of cultured fibroblast-like synovial (FLS) cells and co-cultured dorsal root ganglion (DRG) neurones (a) Mono-culture of FLS cells (b) Co-culture of FLS cells and DRG neurones...

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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt

Danh mục: Báo cáo khoa học

... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...

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Báo cáo y học: "Hypoxic conditions increase hypoxia-inducible transcription factor 2α and enhance chondrogenesis in stem cells from the infrapatellar fat pad of osteoarthritis patients" pps

Danh mục: Báo cáo khoa học

... CD44, CD90 and CD105 (markers for mesenchymal stem cells) , and for CD29 (β1 integrin) The cells stained poorly for LNGFR and STRO-1 (markers on freshly isolated bone marrow stem cells) and sparsely ... Results Isolation, culture and cell surface epitope characterisation of IPFP cells The cells isolated from the IPFP proliferated in culture and reached confluence by day 14 The yield of cells by ... HIF1α is one of the major regulators of hypoxic response in most cells and tissues [44], where it is frequently associated with angiogenesis and the formation of new blood vessels Targets of its molecular...

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Báo cáo y học: "Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer" ppsx

Danh mục: Báo cáo khoa học

... 0.030 and P = 0.029) primary tumor cells Thirty of them (39.49%) have showed higher level of TS in primary tumors than in normal tissues To study the significance of the differential expression of ... as: Liu et al.: Significance of differential expression of thymidylate synthase in normal and primary tumor tissues from patients with colorectal cancer Journal of Hematology & Oncology 2011 ... expression of TS in primary tumors, clinicopathological features, including age, gender, stage, histological grade, tumor site, and the number of lymph nodes examined, were analyzed and compared The results...

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Connecting brain tumor stem cells with their primary tumor and exploring glycogen synthase kinase 3ß regulation of cell fate

Danh mục: Tổng hợp

... similar identification of these cells in clinical specimens has followed the studies involving first, leukemic stem cells2 0 and now several other tumor systems21-24; namely, tumor- initiating capacity ... tumor suppressor” or tumor promoter”59 In certain types of tumors, GSK3β may function as a tumor suppressor”, but enhances the growth and development of yet other kinds of tumors Reports have ... glands promotes mammary tumorigenesis, mediated by dysregulation of the Wnt/β-catenin pathway63 On the other hand, studies have also provided evidence of GSK3β as a promoter of tumorigenesis and...

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báo cáo hóa học:" Stem cells from umbilical cord blood do have myogenic potential, with and without differentiation induction in vitro" pdf

Danh mục: Hóa học - Dầu khí

... the dark After that, cells were washed in PBS 1× and cultured protected from light Stained stem cells were used in co-cultures of DMD muscle cells and normal CD34+ stem cells from umbilical cord ... Co-cultures of CD34+ stem cells and DMD myoblasts showed positive dystrophin when compared with the normal myoblast culture (figure 4) This result suggests that the fusion of stem cells and muscle cells ... 15 days of co-cultures with CD34+ stem cells and DMB affected patient muscle cells (data not shown) In order to confirm if there was any expression of dystrophin from the CD34+ stem cells, we...

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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt

Danh mục: Hóa học - Dầu khí

... progenitor cells (MAPCs), are the stem cells Page of 15 forming bone marrow [33] Their role is maintenance and turnover of blood cells and immune system The high rate of regeneration of the liver, ... Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cells of a patient through ... unrelated to embryonic stem cells Adult stem cells are undifferentiated cells occurring in tissues and organs of adult individuals which are able to convert into differentiated cells of the tissue where...

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Báo cáo sinh học: " Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pot

Danh mục: Hóa học - Dầu khí

... Hematopoietic stem cells (HSCs) contain specific markers on their surface such as CD34 and CD133 allowing for the isolation of relatively pure populations of these cells for in vitro study of the cellular ... study Isolation and culture of hematopoietic stem cells (HSCs) Bone marrow samples (BM) from Hb E/b-thalassemic patients and healthy donors were collected for HSCs isolation Briefly, mononuclear cells ... because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and...

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o cáo hóa học:" Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pptx

Danh mục: Hóa học - Dầu khí

... Hematopoietic stem cells (HSCs) contain specific markers on their surface such as CD34 and CD133 allowing for the isolation of relatively pure populations of these cells for in vitro study of the cellular ... study Isolation and culture of hematopoietic stem cells (HSCs) Bone marrow samples (BM) from Hb E/b-thalassemic patients and healthy donors were collected for HSCs isolation Briefly, mononuclear cells ... because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and...

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Báo cáo y học: "Flow cytometric assessment of the signaling status of human B lymphocytes from normal and autoimmune individuals" doc

Danh mục: Báo cáo khoa học

... percentage of the peripheral blood cells, has been challenging because of the large number of cells needed for biochemical assessment of signaling status and the relatively poor efficiency of transfection ... synthesis of IκB proteins Following engagement of CD40 on Ramos B cells with rCD154, the phosphorylation of IκBα and the degradation of IκBα, -β and -ε were detected by Western Blotting (Fig 2c) and ... (a) and (b) Insets of histograms of fluorescence intensity of the channel that detects the fluorochrome conjugated secondary antibody are shown in (a) and (b) The percentage of positive cells and...

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Báo cáo y học: "Polarized subsets of human T-helper cells induce distinct patterns of chemokine production by normal and systemic sclerosis dermal fibroblasts" doc

Danh mục: Báo cáo khoa học

... capacity of T cells to elicit the production of chemokines by dermal fibroblasts and, in particular, the capacity of polarized T cells (Th1 and Th2) to modulate fibroblast production of chemokines ... presence of resting T cells (Figure 1a) Dose-dependent increases in IL-8, MCP-1 and IP-10 were observed in the presence of activated T cells However, Th1 cells were less potent inducers of IL-8 and ... produced by Th1 cells produced IL-8 and high levels of MCP-1 and IP-10, whereas those exposed to cytokines produced by Th2 cells produced high levels of IL-8 and MCP-1 but marginal amounts of IP-10...

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Báo cáo y học: "Adipose-derived mesenchymal stem cells from the sand rat: transforming growth factor beta and 3D co-culture with human disc cells stimulate proteoglycan and collagen type I rich extracellular matrix" ppsx

Danh mục: Báo cáo khoa học

... concentration and annulus cells adiposederived mesenchymal stem of AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and ... localisation of the multipotent mesenchymal stem cell markers CD105 and CD29 and negative localisation of CD45 and CD34 [23] For cluster of differentiation (CD) immunohistochemical assessment of stem ... mesenchymal stem cells (AD-MSC) offer some advantages as an attractive, readily available adult stem cell because of the ease of harvest and their abundance [11,12] AD-MSC are capable of differentiating...

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Báo cáo y học: " Over-expression of HO-1 on mesenchymal stem cells promotes angiogenesis and improves myocardial function in infarcted myocardium" pdf

Danh mục: Báo cáo khoa học

... tissue of HO-1-MSCs group rather than in the NullMSCs and PBS group Effects of HO-1-MSCs transplantation on angiogenesis Immunofluorescent staining for a-smooth muscle actin and quantification of ... Under most circumstance, the treatment of MI by using MSCs showed poor survival of transplanted cells In addition to the quick loss of cells within 24 h of transplantation caused by cell leakage ... expression of FGF2, VEGF and actin were developed by the ECL method and relative protein levels were quantified by scanning densitometry and the relative gray value of protein = protein of interest/internal...

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Báo cáo khoa học: "Isolation of Aspergillus spp. from the respiratory tract in critically ill patients: risk factors, clinical presentation and outcome" potx

Danh mục: Báo cáo khoa học

... stay before isolation of Aspergillus spp was 32.1 ± 21.4 days Previous use of fluconazole was recorded in eight of the 36 patients (22.2%) with isolation of Aspergillus spp., and in 41 of the 105 ... comparison of categorical variables A comparison of risk factors for the isolation of Aspergillus spp between groups of patients with Aspergillus spp., patients with Candida spp infection, and noncolonized, ... presence of risk factors in the host, isolation of an Aspergillus sp and suggestive clinical or radiological findings; 'possible' aspergillosis requires the presence of risk factors in the host and isolation...

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Báo cáo y học: "Comparative transcriptome analysis of embryonic and adult stem cells with extended and limited differentiation capacity" pps

Danh mục: Báo cáo khoa học

... [1,2] Other adult stem cells include neural stem cells (NSCs) [3], and the mesenchymal stem cells (MSCs) that give rise to osteoblasts, chondrocytes, adipocytes, and skeletal and smooth muscle ... transcriptomes of one clone of rat MAPCs (rMAPC-1) expressing high levels of Oct4 mRNA and protein and one clone of rat cells that expressed 1,000-fold lower levels of Oct4 mRNA and differentiated ... developmental path to neural fate by global expression profiling of mouse embryonic stem cells and adult neural stem/ progenitor cells Stem Cells 2006, 24:889-895 NIA array analysis [http://lgsun.grc.nia.nih.gov/ANOVA/...

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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages

Danh mục: Tổng hợp

... events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of these cells has led to the development of several experimental approaches ... Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes ... Pancreas and diabetes The pancreas consists of Acinar cells which perform its exocrine functions and clusters of cells called Islets of Langerhans which perform its endocrine functions Acinar cells...

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Identifying key signatures of high productivity from transcriptome and proteome profiles of CHO cells

Danh mục: Tổng hợp

... from these cells (Anderson and Krummen, 2002; Chu and Robinson, 2001) To meet market demands, the scale of bio-therapeutic production is usually very large, often in tens of thousands of litres, ... selective media (a) Cells under normal illumination (b) Fluorescent cells 43 Figure 4.3: FACS profile of CHO/dhfr- and CHO-dhfr-GFP after weeks in selective media A shift of profile towards ... the long-term expression of the recombinant gene, the stability of cells was monitored using their FACS profiles and two stable clones producing high and low levels of DHFR-GFP fusion protein...

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Báo cáo y học: "ISOLATION OF CHLAMYDIA PNEUMONIAE FROM SERUM SAMPLES OF THE PATIENTS WITH ACUTE CORONARY SYNDROME"

Danh mục: Y học thưởng thức

... of Termostar Taq DNA polymerase (Syntol, Moscow, RF); and 5pmol of both forward and reverse primers and 3,5 pmol probe The real-time PCR run was 10 at 95°C, and 50 repeats of 20 sec at 95°C and ... lack of standardized criteria for laboratory diagnostics of chronic C pneumoniae infection as well as contradictory information about distribution of the pathogen throughout of the tissues of human ... µl of DNA was added to reaction mixture containing 1x PCR buffer (Silex, Moscow, RF) containing 10 mM Tris-HCl, pH 8,3 , 2.5 mM MgCl2, 15 pmol of each primer, 200 µM of each of dNTPs and U of...

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Báo cáo y học: "Application of Small Angle X-ray Scattering (SAXS) for Differentiation between Normal and Cancerous Breast Tissue"

Danh mục: Y học thưởng thức

... 0.33, 0.36, 0.46 and 0.4 nm for carcinoma, fibrocystic changes, adipose and fibroglandular tissues respectively Comparison of the measured signatures between the fresh and frozen tissues is shown ... student’s t-test, of the change of peak position between different tissues It can be seen that peak position, is a reliable way to differentiate between normal and tumor breast tissues Furthermore, it ... between fibrocystic changes and carcinoma using this parameter The difference between adipose and tumors is further highlighted and even though the fibroglandular and fibrocystic changes peaks...

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