isolation of human brain endothelial cells and characterization of lipid raft associated proteins by mass spectroscopy
báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf
... significance of VCAM-1 and CD11b studies p values < 0.05 were considered significant Results Anandamide inhibits VCAM-1 induced by TMEV in brain endothelial cells by CB1 receptors The endothelial blood brain ... studies and participated in the interpretation of data and revising manuscript draft FC, FL and FD participated in the design of the study, interpretation of data and revision of manuscript draft ... Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors Journal of...
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báo cáo hóa học: " Human brain endothelial cells endeavor to immunoregulate CD8 T cells via PD-1 ligand expression in multiple sclerosis" pdf
... expressed by activated immune cells [16] such as T cells, B cells, macrophages, dendritic cells and microglia [17], as well as by non-immune cells such as endothelial and epithelial cells [18,19], and ... (cytokines and lytic enzyme) of human CD8 T cells Human brain endothelial cells in MS lesions not express PD-L1, while PD-L2 is down-regulated To assess whether endothelial cells in the CNS of MS ... HBECs to reduce the migration and activation of human T cells Our analysis of post-mortem human brain tissues underlines that PD-L2 is expressed by all brain endothelial cells under normal physiological...
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Báo cáo khoa học: " Profile of time-dependent VEGF upregulation in human pulmonary endothelial cells, HPMEC-ST1.6R infected with DENV-1, -2, -3, and -4 viruses" pps
... vascular permeability and maintaining vascular permeability [11] Endothelial cells infected by another viral agent, Hepatitis C virus [16], and epithelial cells infected by human rhinovirus [17] ... Kaposi's sarcoma -associated herpesvirus induces sustained levels of vascular endothelial growth factors A and C early during in vitro infection of human microvascular dermal endothelial cells: biological ... DENV-infected cells showed a higher level of VEGF when compared to mock-infected cells (Table and Fig 2) An interesting display of profile consistent across all four DENV serotypes was observed, whereby...
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Báo cáo y học: " Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells" pdf
... differences in the profile of cytokines induced The efficiency of infection of the cells by the apical route was 70-100% and basolateral route was 30-50% As previously reported by us, there was a ... epithelial cells infected by influenza H1N1 and H5N1 viruses The concentrations of the IP-10, RANTES and IFN β were measured by ELISA in apical and basolateral culture supernatants of type I-like ... number and cell characteristics of the normal human lung Am Rev Respir Dis 1982, 125(6):740-745 Thompson CI, Barclay WS, Zambon MC, Pickles RJ: Infection of human airway epithelium by human and...
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Experimentation, modeling and control of calcium dynamics in human vascular endothelial cells
... of cells lining the inner wall of blood vessel and works as a barrier separating the blood flow and vascular muscle cells As continuously exposed to the flowing blood, vascular endothelial cells ... 1.2.2 Views of Engineers By far we have a clear picture of one typical signaling pathway in human VECs It starts from shear stress generated by blood flow and ends with the production of one significant ... human vascular endothelial cells (VECs), the dynamic behavior of intracellular calcium, i.e., its temporal/spatial variation, will directly affect cell proliferation, synthesis and secretion of...
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Investigation of relative expression level of SLC4 bicarbonate transporter family in mouse and human corneal endothelial cells
... Mouse corneal endothelial cells culture 24 2.5 Human corneal endothelial cells culture .25 2.6 RNA isolation (from corneal endothelium and cultured cells of MCECs and HCECs) 26 ... obtain another batch of cells at passage and 2.5 Human corneal endothelial cells culture The HCECs were kindly provided by Dr Gary Peh Swee Lim of Ocular Tissue Engineering and Stem Cell group ... passage and passage were used for subsequent RNA isolation 2.6 RNA isolation (from corneal endothelium and cultured cells of MCECs and HCECs) The Descemet’s membranes with corneal endothelial cells...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... di- and tri) methylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; H3K36me3, trimethylation of lysine 36 of ... concomitantly modified by the trimethylation of lysine of histone H3 (H3K4me3), a modification generally associated with transcriptional initiation, and trimethylation of lysine 27 of histone H3 (H3K27me3), ... quality and differentiation potential of individual lines [91,95] The second source of variability is a natural consequence of the genetic variation among human cells or embryos from which iPSCs and...
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Báo cáo y học: "Apremilast, a novel PDE4 inhibitor, inhibits spontaneous production of tumour necrosis factor-alpha from human rheumatoid synovial cells and ameliorates experimental arthritis" docx
... concentration (IC50) of 74 nM and inhibits TNFα production from lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMC) and whole blood by 7.7 nM and 11 nM, respectively ... FMcC drafted the manuscript and analysed and interpreted the data AP acquired the data from LPS-stimulated monocytes and RA synovial cells, and contributed to drafting of the manuscript MA carried ... different forms of established experimental arthritis Disease severity was evaluated throughout, followed by histological assessment of the extent of joint inflammation and erosion at the end of the treatment...
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Báo cáo y học: " Differential effects of cigarette smoke on oxidative stress and proinflammatory cytokine release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells" potx
... evidence of apoptosis in human lung cancer cells (H1299) Figure Cigarette smoke extract caused necrosis with no or little evidence of apoptosis in human lung cancer cells (H1299) Human lung cancer cells ... stress and proinflammatory cytokine release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells Respir Res 2006, 7:132 Publish with Bio Med Central and ... little evidence of apoptosis in primary human small airway epithelial cells Cigarette Cigarette smoke extract caused necrosis with no or little evidence of apoptosis in primary human small airway...
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Báo cáo y học: " Differential effects of cigarette smoke on oxidative stress and proinflammatory cytokine release in primary human airway epithelial cells and in a variety of transformed alveolar epithelial cells" pps
... from either human or rodents To test our hypothesis, we used a panel of human and rodent alveolar epithelial cell lines, such as human lung cancer cells (H1299), human lung epithelial cells (H441), ... variety of alveolar epithelial cells and in primary human small airFigure way epithelial cells Cigarette smoke extract differentially caused cytotoxicity in a variety of alveolar epithelial cells and ... cells and in primary human small airway epithelial cells A Various alveolar epithelial cells such as human lung cancer cells (H1299), human adenocarcinoma cells (A549), human lung epithelial...
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Báo cáo y học: "Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules" ppt
... http://www.retrovirology.com/content/5/1/86 Figure Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 expression in A549 cells cocultured with MT-2 cells Secretion of IL-8 and CCL20 and Induction of cell surface ICAM-1 ... cytoplasm of epithelial cells (A and D), and infiltrated lymphocytes (B and E) and macrophages (C and F) HTLV-I can be transmitted into lung epithelial cells from infected T cells and the integrated ... investigated the production of IL-8 and CCL20 by A549 cells cocultured with MT-2 or CCRF-CEM cells A549 cells cocultured with MMC-treated MT-2 cells released considerable amounts of IL-8 and CCL20 (Figure...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... specification of the neuroectoderm (Muñoz-Sanjuán and Brivanlou, 2002) Bmp are secreted from roof plate cells in the dorsal neural tube and induce formation of neural crest stem cells and dorsal ... the study of SHH signaling in hESC offers to augment the current understanding of the role of SHH in humans, that is based largely on the mouse model Figure 2.6 Human embryonic stem cells (hESC) ... state of ESC and the loss of their expression leads to ESC differentiation NANOG, named after the mythical Celtic land of Tir nan Og, is expressed in the inner cell mass of the early embryo and...
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Atomic force microscope imaging of human red blood cells and theri mechanical properties
... illustration of the method of operation of an AFM The position of the cantilever of the AFM is controlled by three piezo-ceramic controllers, which place the tip of the AFM in the x and y directions and ... modulus of points A, B, C, D, and E of a swollen RBC in PBS solution 59 Figure 28 Cross section profile of a fresh RBC in PBS solution 60 Figure 29 Force curves of A, B, C, D, and E of a fresh ... deformation, and shape of cells, leading to RBC pathophysiology It is important to have a basic understanding of the anatomy of the RBC membrane skeleton for a clear perception of how these elements and...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... studies of LIN28 19 20 Objective of the current study Page II Materials and methods 22 2.1 Culture of human ES cell line 22 2.1.1 Preparation of feeder cells 22 2.1.2 Maintenance of human ES cells ... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... assessment of the differentiation status of human ES cells (Bhattacharya et al., 2005) Analysis of expression profile of undifferentiated, partially differentiated and differentiated human ES cells...
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Báo cáo Y học: Group IID heparin-binding secretory phospholipase A2 is expressed in human colon carcinoma cells and human mast cells and up-regulated in mouse inflammatory tissues doc
... portions of mouse and human sPLA2-IIAs and rat and human sPLA2-Vs serve as functional heparin-binding sites [7,8,34,36], we replaced some of these cationic residues in mouse sPLA2-IID with neutral and/ or ... KE2 and KE2RS2 Two and four basic amino acids are replaced by glutamic acid or serine in KE2 and KE2RS2, respectively (B) Expression of the wild-type (WT) and two mutants of mIID in HEK293 cells, ... (spleen and thymus) and digestion (small and large intestines) of both human and mouse [21,22], which types of cell express this sPLA2 isozyme remains obscure We therefore surveyed the expression of...
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Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf
... prepared the human modified synthetic oviductal fluid (SOF) with amino acids (hmSOFaa) by supplementing mSOFaa with human serum Establishment and characterization of human cloned ES cells Having ... Gokhale PJ, Andrews PW Culture and characterization of human embryonic stem cells Stem Cells Dev 2004, 13, 325-336 18 Drukker M, Benvenisty N The immunogenicity of human embryonic stem-derived cells ... components and to culture hES cells on feeder-free conditions or human feeder cells for safe transplantation of human ES cells The use of feeder-free systems, such as Matrigel or other components of...
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Báo cáo y học: "HIV-1 Capsid Assembly Inhibitor (CAI) Peptide: Structural Preferences and Delivery into Human Embryonic Lung Cells and Lymphocyte" pptx
... N-terminus of p55 is myristoylated triggering its association to the cytoplasmic side of cell membranes [7] and the release of the budding of viral particles from the surface of infected cells After ... a set of smaller proteins and spacer peptides (SP) encoded by the viral pol gene during the process of viral maturation The proteins are termed: matrix p17 (MA), nucleocapsid p9 (NC), p4 and capsid ... through membranes and understand better their pharmacological behaviour For delivery of the HIV-1 CAI into human cells a bi-modular peptide was developed and constructed consisting of a transport...
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Báo cáo y học: " C-reactive protein does not opsonize early apoptotic human neutrophils, but binds only membrane-permeable late apoptotic cells and has no effect on their phagocytosis by macrophages" pot
... transfected and non-transfected cells There have been several reports of Ca2+-dependent opsonisation of apoptotic cells by pentraxins [9,11,21] The lack of CRP binding to classical early apoptotic human ... single bands of approximately 23 kD in SDS-PAGE (b) Western blot of CRP and known amounts of human IgG on a 9% acrylamide gel under nonreducing conditions The blot was probed with anti -human IgG-HRP ... using indirect immunofluorescence and flow cytometry confirmed expression of FcγRIIA by the transfected cells, but no expression of FcγRI or FcγRIII was detected Control Jurkat cells transfected...
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Báo cáo y học: " Endothelial cells and pulmonary arterial hypertension: apoptosis, proliferation, interaction and transdifferentiation" ppt
... (SM-MHC) in non-SMC lineage cells [74] SU5416 expanded the number of CD34 and/ or c-kit positive cells and caused transdifferentiation of CD34+ cells, but not CD34- cells In conclusion, this data ... conceived of the report, contributed to its design and conception and drafted the manuscript KT drafted the manuscript and contributed to its design and conception NV contributed to its design and drafted ... muscle-like cells Endothelial cell death induced by VEGF receptor blockade and subsequent selection of progenitor-like cells leads to transdifferentiation to smooth muscle-like cells and neuronal...
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Báo cáo sinh học: " Improved gene delivery to human saphenous vein cells and tissue using a peptide-modified adenoviral vector Lorraine M Work1, Paul N Reynolds2 and Andrew H " pptx
... Vaccines and Therapy 2004, 2:14 http://www.gvt-journal.com/content/2/1/14 Figure Transduction of saphenous vein cells and intact tissue Transduction of saphenous vein cells and intact tissue Ad-CTL and ... vectors transduce both endothelial and smooth muscle cells during graft Page of (page number not for citation purposes) Genetic Vaccines and Therapy 2004, 2:14 gene delivery [9], and here show increased ... therapy of human vascular bypass grafts with E2F decoy: the PREVENT single-centre, randomised, controlled trial The Lancet 1999, 354:1493-1498 Baker AH, Mehta D, George SJ, Angelini GD: Prevention of...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008