interaction of electrons with atoms

... value of the amplitude of the states of O FI ¼ 0, with respect to the initial state jC I i at t ¼ 0, increases with increasing t, while the amplitudes of the states of O FI 6¼ 0 oscillate without substantial ... V dN dE ¼ V hh 1 2cp  3 ðn 2 r n g Þ! 2 dO ð2:59Þ Interaction of Electrons and Photons 31 Copyright © 2004 Marcel Dekker, Inc. 2 Interaction of Electrons and Photons This chapter provides the basis ... existence of eigenstates of infinitively large negative n. Since such eigenstates are not Interaction of Electrons and Photons 21 Copyright © 2004 Marcel Dekker, Inc. The Hamiltonian for the energy of...

Ngày tải lên: 23/10/2013, 20:15

... spectra of a recombinant decorin purified in the absence of chaotropic agents, with the exception of the wavelength of the minimum (215–216 instead of 218 nm) and very different to the spectrum of ... 2002 Interaction of decorin with collagen peptides (Eur. J. Biochem. 269) 1431 with respect to the unmodified ones is not related to the percentage of Lys/Hyl side chains that did not react with ... these data demonstrate the essential role of the positive charge of collagen Lys/Hyl residues for interaction with decorin. DISCUSSION The binding of decorin with fibrillar collagens has been extensively...

Ngày tải lên: 21/02/2014, 15:20

... for the interaction of frog annexin A1 and annexin A2 with dicalcin. Consistently, we observed that the 35 kDa forms of annexin A1 and annexin A2 found in the fraction of the binding proteins of dicalcin (Fig. ... presence of dicalcin and all of the annexins (E) was compared with the calcu- lated sum of each of the initial rates obtained in (B)–(D) (thick dot- ted lines). T. Uebi et al. Role of dicalcin ... 4869 Comprehensive interaction of dicalcin with annexins in frog olfactory and respiratory cilia Tatsuya Uebi 1 , Naofumi Miwa 1,2, * and Satoru Kawamura 1,2 1 Department of Biology, Graduate School of Science,...

Ngày tải lên: 07/03/2014, 05:20

Ngày tải lên: 16/03/2014, 23:20

Ngày tải lên: 23/03/2014, 03:20

Ngày tải lên: 23/03/2014, 04:21

Ngày tải lên: 31/03/2014, 21:21

... into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp Vasthi ... spite of the rel- atively low agmatinase activity of the Asn149Asp vari- ant, it is clear that the interactions of arginase II with l-arginine and agmatine are greatly altered by replace- ment of ... blue staining of purified enzymes. Metal-free species of purified enzymes were obtained by incubation for 1 h at 25 °C with 25 mm EDTA and 3 m V. Lo ´ pez et al. Interaction of arginase II with substrate...

Ngày tải lên: 20/02/2014, 02:21

... intensity of the band of unhydrolyzed actin was plotted against the time of incubation. Characterization of actin preparation quality Fluorescence parameters were used for estimation of nativity of ... aggregation of partially folded actin. To obtain more detailed information on the interaction of HSP25 with partially unfolded actin, we analyzed the effect of different quantities of HSP25 on ... parameter A of actin was close to 2.4. Increase of the time of heating at 43 °C leading to decrease of parameter A up to 2.2–2.3 was accompanied by further decrease of the initial rate of polymerization....

Ngày tải lên: 20/02/2014, 23:20

... basal levels of resonance units (GlnK free surface) within seconds. To test the effects of various molecules on the interaction of TnrA with GlnK, 40 nm GlnK (trimer) Fig. 1. Coimmunoprecipitation of TnrA, ... equilibrated with 10 column volumes (10 · 0.2 mL) of buffer B, with subsequent washing four times with five volumes of the same buffer. Proteins were eluted with buffer E (buffer B supplemented with 250 ... (SPR) of the GlnK–TnrA interaction As a next step, the interaction of TnrA with GlnK was investigated in vitro by BIAcore SPR detection. For this analysis, a Strep-tag II-tagged variant of GlnK (GlnK-ST)...

Ngày tải lên: 06/03/2014, 00:21

... Recognit. 16, 91–101. Ó FEBS 2004 Interaction of human TCR with superantigen SSA (Eur. J. Biochem. 271) 4083 Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen ... Vb5.2 and V b1withK d of 9–11 l M with a fast k ass and a moderately fast k diss . In spite of the reported stimu- lation of V b2.1 bearing T-cells by SSA, we o bserved no measurable interaction. Keywords: ... Consequently, the molecular studies of the interaction of SAgs with their s pecifics ligands will not only advance understanding of the physiological mechanisms of these molecules, but may lead to...

Ngày tải lên: 07/03/2014, 16:20

... report on the interaction of an N-terminal (E2(7–26)) and an internal (E2(279–298)) synthetic peptide sequence of the E2 structural pro- tein of HGV ⁄ GBV-C with phospholipid membranes of different ... short hydrophobic segments of viral envelope glyco- proteins with a very low content of hydrophilic amino acids, the presence of acidic residues in the fusion pep- tides of some low-pH-activated ... strongly interacted with PtdCho ⁄ PtdSer (65 ⁄ 35) vesicles, as the blue shift of Trp was 11 nm. To study the contribution of electrostatic inter- actions to the binding of both peptides with negatively charged...

Ngày tải lên: 07/03/2014, 17:20

... study, we measured the interaction of stefin B with various combinations of phospholipid monolayers and bilayers. Interaction of stefin B in the prefibrillar aggregated state with model lipid membranes ... channel hypothesis of AD and neurodegeneration in general, is not incompatible with other key elements of toxicity, as, for example, the deregulation of Ca 2+ homeostasis and generation of reactive ... membranes, initiating a cascade of detrimental events for the cell. Interaction of granular aggregates and globular oligo- mers of an amyloidogenic protein, human stefin B, with model lipid mem- branes...

Ngày tải lên: 07/03/2014, 17:20

... predicted protein is composed of 306 amino acids, with a calculated molecular mass of 34.3 kDa and a pI of 5.72. Similar searches with the deduced amino-acid sequence of f ull-length Calliphora AFP, ... according to the following scheme: 3 · 10 min with 4 · NaCl/Cit; 2 · 10minwith2· NaCl/Cit; 1 0 min with 0.1 · NaCl/Cit; 10 min with 0.05 · NaCl/Cit; 5 min with NaCl/Tris. After incubation for 30 m ... encoded a protein with 93% identity in the deduced amino-acid sequence with the A FP of S. peregrina (GenBank accession number BAA99282). Because of the high sequence identity with the Sarcophaga...

Ngày tải lên: 08/03/2014, 22:20

... ) * k on;1 k off;1 AB ð2Þ AB þ B ) * k on;2 k off;2 AB 2 ð3Þ where d½B=dt ¼À2k on;1 ½A½Bþk off;1 ½ABÀk on;2 ½AB[B] þ 2k off;2 ½AB 2 ð4Þ d½AB=dt ¼ 2k on;1 ½A½BÀk off;1 ½AB À k on;2 ½AB[B] þ 2k off;2 ½AB 2 ð5Þ d½AB 2 =dt ... that mutation of Ca 2+ ligating amino acids in the N-terminal part of the first EGF-like domain of factor X influences the amidolytic activity of the intact protein [36]. However, direct interactions ... vicinity of the membrane. We found that the isolated factor X Gla domain exhibits low affinity binding to PtdSer-containing membranes with a K d of 4.6 l M . This agrees well with the value of 2.4...

Ngày tải lên: 08/03/2014, 23:20

... behavior of trehalase depending on the nature of the activation stress (Fig. 5B). This shift might argue against the participation of a unique set of interactions involving association of Ntp1p ... identification of strains C3 and C69, with one copy of Ntp1p–Ha6H expressed from the genomic ntp1 + promoter, was verified by Southern blot analysis and immunoblot of whole-cell extracts with anti- Ha ... the regulation of Ntp1p activity under stress relies on the existence of some kind of interaction between Ntp1p and Tps1p, or other elements, that may be lost under the conditions of the in vitro...

Ngày tải lên: 08/03/2014, 23:20

... sequence similarity with MPs of other genera, but showed 32% identity with the MP of South- ern bean mosaic virus within the Sobemovirus genus. With a view to understanding the mechanism of cell-to-cell ... similarity with MPs of viruses from other gen- era. Within sobemoviruses, the sequence of the SeMV MP was closest to that of the SBMV-Ark MP (32% sequence identity), and the identity with MPs of other sobemoviruses ... in Fig. 8A,B). These results show that the interaction of A B Fig. 7. Mapping of the SeMV MP domain responsible for interaction of MP with NV. (A) Purification of GST–MP deletion mutants. GST–MP deletion...

Ngày tải lên: 15/03/2014, 00:20

... dissociation was observed with the A 8 B 8 isoform of GAPDH lead- ing to the A 2 B 2 isoform with a higher NADPH- dependent activity [39]. By analogy with the CTE of the isoform of GAPDH [15,37], we ... date, mapping of the interaction site of CP12 had not studied but our results give some clues with regard to the binding of CP12 to GAPDH. The impact of CP12 binding on the activity of GAPDH is ... dissociation results in the release of PRK as a dimer, whereas GAPDH remains strongly associated with CP12 and forms a subcomplex of one tetramer of GAPDH with one monomer of CP12 [2,10]. However, in...

Ngày tải lên: 16/03/2014, 13:20

... were washed twice with buffer C and twice with buffer C with- out NP40. The elution was made with three volumes of buffer C without NP40 adjusted to 1.5 m NaCl, incubating for 10 min with gentle stirring. ... nuclear-enriched fraction of proteins. The effi- ciency of labelling of the single oligomers being not homogenous, the ratios of the different binding signals within each lane instead of their absolute ... the amount of recovered protein owing to the presence of a high molar excess of BSA remaining in the recovery buffer. Therefore, a fixed aliquot of the protein was incubated with 1 ng of the indicated...

Ngày tải lên: 16/03/2014, 13:20

Ngày tải lên: 16/03/2014, 14:20