inflammation expression of pro inflammatory cytokines formation of leukotriene b4 and tumor promotion in mouse skin by boswellia serrata extracts
Báo cáo y học: "The proinflammatory cytokines IL-1β and TNF-α induce the expression of Synoviolin, an E3 ubiquitin ligase, in mouse synovial fibroblasts via the Erk1/2-ETS1 pathway" ppt
... upregulation of SYN expression is involved in the development of CIA in mice Induction of SYN expression by IL-1β and TNF-α in mouse synovial fibroblasts The production of proinflammatory cytokines by ... first examined the Page of 10 (page number not for citation purposes) Figure Induction of Synoviolin (SYN) expression by proinflammatory cytokines expression by proinflammatory cytokines (a) Mouse ... was induced by intradermal injection of DBA1 mice with 50 μl of emulsion containing 100 μg of collagen in CFA On day 21, the mice were boosted by intradermal injection with 100 μg of collagen in...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick lines, + saponin) cells cultured without and with iron as indicated (D) Perls’ staining of cytospins of ... caused by iron loading has an impact in protein integrity, as indicated by an increase in protein-bound acrolein adducts at the cell surface, which point to acrolein-adducts as a reliable marker of ... in iron-loaded HepG2 cells Determination of phosphatidylserine expression and cell death Phosphatidylserine expression on the outer part of the plasma membrane was examined by Annexin V binding...
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báo cáo hóa học: " Pharmacological inhibition of Akt and downstream pathways modulates the expression of COX-2 and mPGES-1 in activated microglia" potx
... increases the production of prostaglandins It has been shown that mTOR inhibitors reduced NOS activity and iNOS expression induced by hypoxia and a mixture of cytokines in BV-2 cells and primary ... by pharmacological inhibition of these pathways in regard to the expression of cyclooxygenase (COX)-2 and microsomal prostaglandin E synthase-1 (mPGES-1) and to the production of prostaglandin ... phosphatidylinositol 3-kinase, mammalian target of rapamycin, glycogen synthase kinase-3, Akt, prostaglandins FINDINGS Inflammation has been recognized not only as a mere bystander in neurodegenerative...
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Báo cáo hóa học: "Expression of 14-3-3 protein isoforms in mouse oocytes, eggs and ovarian follicular development" pdf
... switching The proteins bind to a diverse set of target proteins and alter cellular function by binding to and causing conformational changes in target proteins or modifying target protein interactions ... of proteins Seven mammalian isoforms of 14-3-3 are known (β, γ, ε, ζ, η, τ and σ) These proteins associate with many intracellular proteins involved in a variety of cellular processes including ... (using standard Western blotting procedures), each testing for one of the seven isoforms in protein extracts Oocytes and eggs were rinsed in MEM, counted, and transferred to Tris-buffered saline...
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Báo cáo y học: "Expression of 5-lipoxygenase and 15-lipoxygenase in rheumatoid arthritis synovium and effects of intraarticular glucocorticoids" docx
... through inhibition of 5-LO enzyme and reduced formation of its proinflammatory products Together with previous studies incriminating LTB4 as a potent mediator of joint inflammation and destruction in ... fer proinflammatory actions by increasing vascular permeability in vitro [19], enhancing expression of monocyte chemotactic protein-1 and TNFα in vascular smooth muscle cells via activation of ... pathway could provide additional benefits in the treatment of RA, by reducing the formation of LTB4 and, by this means, decreasing the chemotaxis of inflammatory cells Few studies have investigated...
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Báo cáo khoa học:" Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pptx
... only PBMC (VCRT Kinetic of the expression of p24gag in supernatants of cotton rat cell clones after HIV-1 infection Figure Kinetic of the expression of p24gag in supernatants of cotton rat cell ... supernatant of infected cells, [2] detection of episomal viral cDNA in infected cells, [3] demonstration of viral DNA integration into the genome of the cells, and [4] production of infectious ... levels of hCD4 expression in the CCRT K4 clone, most likely due to the silencing effect of chromatin on the integrated promoter, we enhanced the expression of the receptors by incubating the...
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Báo cáo khoa học: " Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection" pdf
... only PBMC (VCRT Kinetic of the expression of p24gag in supernatants of cotton rat cell clones after HIV-1 infection Figure Kinetic of the expression of p24gag in supernatants of cotton rat cell ... supernatant of infected cells, [2] detection of episomal viral cDNA in infected cells, [3] demonstration of viral DNA integration into the genome of the cells, and [4] production of infectious ... levels of hCD4 expression in the CCRT K4 clone, most likely due to the silencing effect of chromatin on the integrated promoter, we enhanced the expression of the receptors by incubating the...
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Báo cáo sinh học: " Effects of recombinant adenovirus-mediated expression of IL-2 and IL-12 in human B lymphoma cells on co-cultured PBMC" pot
... especially against low immunogenic tumors [4] Interleukin-2 (IL-2) and interleukin-12 (IL-12) are cytokines that elicit strong antitumor effects by stimulating immune cells, including T cells and natural ... donors in comparison with recombinant cytokines as controls Our data indicates that adenoviral expressed cytokines were equally effective compared with recombinant cytokines in enhancing the proliferation ... of incubation, 50 ng/ml of an antibody against CD3, 100 U/ml interleukin-1 (IL-1), and 300 U/ml interleukin-2 (IL-2) (PromoCell, Heidelberg, Germany) were added Cells were incubated at 37°C in...
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Báo cáo y học: " Effects of pro-inflammatory cytokines on expression of kynurenine pathway enzymes in human dermal fibroblasts" pdf
... mechanism and as a means of modulating the immune response [1,21] The enzyme indoleamine 2,3dioxygenase (IDO1) is the first and rate-limiting step of this pathway and is highly induced by the pro- inflammatory ... 20-090 Lublin, Poland 4Department of Toxicology, Institute of Agricultural Medicine, Lublin, Jaczewskiego 2, 20-950 Lublin, Poland 5Department of Physiology and Pharmacology, Karolinska Institutet, ... transcripts encoding enzymes in the kynurenine pathway in human skin- derived fibroblasts from individuals Transcripts encoding the following enzymes were investigated; Indoleamine 2,3-dioxygenase...
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Báo cáo y học: "Effects of pro-inflammatory cytokines on expression of kynurenine pathway enzymes in human dermal fibroblasts" pot
... mechanism and as a means of modulating the immune response [1,21] The enzyme indoleamine 2,3dioxygenase (IDO1) is the first and rate-limiting step of this pathway and is highly induced by the pro- inflammatory ... 20-090 Lublin, Poland 4Department of Toxicology, Institute of Agricultural Medicine, Lublin, Jaczewskiego 2, 20-950 Lublin, Poland 5Department of Physiology and Pharmacology, Karolinska Institutet, ... transcripts encoding enzymes in the kynurenine pathway in human skin- derived fibroblasts from individuals Transcripts encoding the following enzymes were investigated; Indoleamine 2,3-dioxygenase...
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Báo cáo y học: "Polyphenol-rich pomegranate fruit extract (POMx) suppresses PMACI-induced expression of pro-inflammatory cytokines by inhibiting the activation of" ppt
... contain potent inflammatory mediators, including histamine, heparin, proteinases, leukotrienes, and multifunctional cytokines, and their potential contributions to processes of inflammation and ... expression of pro- inflammatory cytokines To determine whether the inhibition of IL-6 and IL-8 expression was mediated by inhibition of MAPK, we examined the effect of POMx on the activation of MAPKs in ... connective tissue, and mucous glands, resulting in the recruitment of activated immune and inflammatory cells to the site of inflammatory lesion, thereby amplifying and sustaining the inflammatory...
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báo cáo hóa học: " Increased levels of HMGB1 and pro-inflammatory cytokines in children with febrile seizures" pot
... multifunctional anti -inflammatory cytokine produced by monocytes, macrophages, lymphocytes, as well as microglia and inhibits the production of proinflammatory cytokines, including IL-1, IL-6, IL-8, and TNF-a ... (20080918/06-2008-74/76) Informed consent was obtained from each child’s parents Cytokine measurement Levels of pro- inflammatory cytokines including HMGB1, IL-1b, IL-6, interferon (IFN)-b, TNF-a, and anti -inflammatory ... significant elevation of HMGB1 in the serum of febrile seizure patients Moreover, serum levels of other pro- inflammatory cytokines, including IL-1b, IL-6, and the antiinflammatory cytokine IL-10 were...
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Proteomics analysis of pro inflammatory cytokine stimulated human lung fibroblasts and bronchial epithelial cells
... fibronectin, laminin, proteoglycans and glycoproteins Their inhibitors, named tissue-specific inhibitors of metalloproteinases (TIMPs) are another family of proteins which modulate MMPs activity in ... shown in airway remodeling by promoting the production of collagen, fibronectin and hyaluronan (Hetzel et al., 2005; Wilkinson et al., 2004), and expressing MMP-1, MMP-3 and MMP-9, and tenascin by ... downstream-regulated gene-1 (NDRG-1), serine protease inhibitor, clade B, member (SERPINB1), cytoskeleton protein keratin10, tropomyosin isoform and γ-actin Interestingly, apolipoprotein L2 (apoL2) was found...
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Báo cáo khoa học: Pulchellin, a highly toxic type 2 ribosome-inactivating protein from Abrus pulchellus Cloning, heterologous expression of A-chain and structural studies ppt
... potent plant toxin, similar to abrin and ricin Cloning of the coding gene from pulchellin A-chain will greatly facilitate the understanding of the protein structure and function, and lay a solid ... and Trp198 in abrin-a and abrin-c, and Tyr115, Tyr158, Glu212, Arg215 and Trp246 in ricin) and another ve conserved residues (Asn72, Arg124, Gln160, Glu195 and Asn196 in abrin-a and abrin-c and ... Cloning and characterization of a curcin gene encoding a ribosome inactivating protein from Jatropha curcas DNA Seq 14, 311317 33 Lord JM (1985) Precursors of ricin and Ricinus communis agglutinin...
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt
... lL of the enzyme/inhibitor mixture to 180 lL of substrate (4NPGlc), incubating for 30 at 37 °C, and measuring the release of 4NP Protein assays and protein sequencing Total protein in crude and ... following gradients were used: incubations containing arbutin or salicin as substrate; 100% C initial, increasing D to 20% (10 min), 50% (15 min), 100% (5 min), held at 100% (5 min); cyanodin glycosides; ... B/95% A initial (5 min), increasing B to 20% (10 min), 90% (10 min), held at 90% (5 min); iso¯avonoid, mandelonitrile and dihydrochalcone glycosides, 17% A/83% B initial (1 min), increasing B to...
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Báo cáo khoa học: Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme pdf
... against buffer A Characterization of the recombinant laccase Protein analysis Protein concentration was determined according to Lowry et al [23] with bovine serum albumin as standard Protein ... performed using antibodies raised against the P cinnabarinus laccase The Western blot analysis showed a unique band corresponding to the 70-kDa protein demonstrating that this protein is the recombinant ... 0.5% of the molecular mass of the native laccase and and 10% for the recombinant laccase In the heterologous production of the P cinnabarinus laccase in P pastoris [14] or the T villosa laccase in...
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Báo cáo khoa học: Thyroid hormone induces the expression of 4-1BB and activation of caspases in a thyroid hormone receptor-dependent manner pptx
... cells in 6-cm diameter dishes were incubated in DME containing 2% FBS that had been dialyzed against saline and 40 lCi of a mixture of [35S]methionine and [35S]cysteine (1000 Ci mmol)1; pro- mix ... that T3 induces apoptosis, which is mediated by 4-1BB, and subsequent activation of caspases in the tissues and organs in the body Impairment of cell proliferation by T3 was more profound in FRTL ... late responses We also examined the expression of TRAF1 and TRAF2 because they interact and regulate the function of the 4-1BB proteins to induce NF-jB [18] In the absence of T3, neither HeLa nor...
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Báo cáo khoa học: Increased sensitivity of glycogen synthesis to phosphorylase-a and impaired expression of the glycogen-targeting protein R6 in hepatocytes from insulin-resistant Zucker fa ⁄ fa rats pptx
... translocation of these proteins [26–28] The expression of GL and PTG, but not R6, in rat liver in vivo is insulin-dependent It declines during insulin deficiency and is restored by insulin treatment [22,40] ... [7,11,20] GL is one of four glycogen-targeting proteins expressed in liver [21–25] These proteins have binding sites for protein phosphatase-1 (PP1) and for glycogen, and they differ in their relative ... previous findings [38] R6 protein was increased by 75% after combined culture with leptin and insulin (Fig 8B) Discussion The Zucker fa ⁄ fa rat is widely used as a model for insulin resistance and type...
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Báo cáo khoa học: Cloning of a rat gene encoding the histo-blood group A enzyme Tissue expression of the gene and of the A and B antigens potx
... determined using the TMHMM, TMAP and TOPPRED2 programs Determination of the exon/ intron boundaries were obtained by analysis of the rat genomic sequences available in the NCBI database The programs ... distribution of the Abo mRNA and of the A and B antigens in the BDIX rat Transcripts were detected by RT-PCR from total RNA extracts of various rat tissues using specific primers An indication of the intensity ... preparation Protein concentration was determined using bicinchoninic acid The reaction mixture contained: 50 lg protein extracts, 30 mM MnCl2, mM ATP, 10 mM NaN3, mM 2¢ fucosyllactose and 20 lM...
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Báo cáo sinh học: " Reduced expression of Jak-1 and Tyk-2 proteins leads to interferon resistance in Hepatitis C virus " docx
... reduced interferon signaling in these replicon cells, expression of JakStat proteins of interferon was examined Interferon signaling occurs through a series of protein-protein interactions that initiate ... colonies in a medium containing G-418, sustained viral RNA replication and protein expression All nine replicon cell lines were cultured in a medium containing mg/ml of G-418 in the presence of cyclosporineA ... obtained with 17-1, 17-2 and 17-3 (Fig 6B) and 24-1, 24-2, 24-3 (Fig 6C) The receptor binding studies were also confirmed by examining expression of interferon receptors using protein lysates by...
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