indirect bystander activation of inkt cells by gram negative lps positive bacteria
Báo cáo khoa học: Sustained activation of ERK1/2 by NGF induces microRNA-221 and 222 in PC12 cells pdf
... variety of important biological responses, the significance of ERK signaling in terms of regulating miRNA expression is of great interest To study the role of the ERK1 ⁄ pathway in the regulation of ... our study showed that the sustained activation of ERK1 ⁄ by NGF, but not the transient activation of ERK, could effectively induce miR221 and 222 in PC12 cells Finally we identified the BH3-only ... NGF induces expression of the microRNAs miR-221 and 222 in PC12 cells (A) NGF-induced differentiation of PC12 cells (B) Schematic representation of the genomic structure of miR-221 and 222 and...
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Báo cáo Y học: Growth inhibition of mammalian cells by eosinophil cationic protein pptx
... 10 lM Effect of ECP on A431 and K562 cells At lM ECP, the growth of K562 cells was completely suppressed whereas growth inhibition of A431 cells was % 50% (Fig 2) As ECP is a member of the supergene ... growth inhibition of K562 cells in Fig was shown after days of treatment with ECP, which was Fig Suppression of cell growth in the presence of ECP Left, the percentages of viable cells under various ... demonstrate that ECP impairs the growth of cells Cellular localization of ECP Fig Growth-inhibitory eect of ECP on K562 cells (A) Cells were seeded at 25 000 cells per 35-mm dish Simultaneously,...
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Báo cáo khoa học: Examining multiprotein signaling complexes from all angles The use of complementary techniques to characterize complex formation at the adapter protein, linker for activation of T cells pdf
... formation of LATmediated signaling complexes play a complex role in the differentiation and homeostasis of T-cell populations, the maturation of B cells and the activation of mast cells by the FceR1 ... phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells Biochem J 356, 461–471 5433 Investigation of multiprotein signaling ... the phosphorylation of these sites is still 5427 Investigation of multiprotein signaling complexes Fig Structure and membrane localization of the linker for activation of T cells (LAT) LAT contains...
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Báo cáo khoa học: Activation of cathepsin D by glycosaminoglycans docx
... activation of the enzyme by cleavage of residues 1–26 had occurred prior to the addition of MH Because pseudoCD lacks residues 1–26 of the prodomain, the result suggested that other regions of ... complete activation of proCD by heparin Effect of heparin on the activity of mature CD The results suggested that residues 1–26 of the prodomain were not necessary for heparin-induced stimulation of ... be less important Effect of heparin on autocatalytic cleavage of proCD The activation of proCD at acidic pH in vitro involves the autocatalytic removal of residues 1–26 of the prodomain [20,21,23]...
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Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
... purification of recombinant hGK The wild-type and mutant forms of hGK isoform were expressed as GST fusion proteins Expression in Escherichia coli (BL21) cells at 28 °C and purification of proteins by glutathione ... binding of the cosubstrate (MgATP2)) and thus catalysis Moreover, the maximal activation of rat liver glucokinase by Glc and the related overall conformational transition, as followed in real-time by ... approach of molecular dynamics studies by real-time ITF spectroscopy, structural dynamic analyses and functional mutagenesis Our findings provide new insight into the catalytic activation of hGK by...
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Báo cáo khoa học: "Altered maturation of dendritic cells by taxol, an anticancer drug" ppt
... cells were stained by propidium iodide for the detection of DC necrosis Annexin V -positive/ PI -negative, annexin Vpositive/PI -positive, annexin V -negative/ PI -positive cells represent cells in early ... number and viability of T cells were measured by trypan blue exclusion test Assessment of cytotoxicity by MTT Assay The viability of DCs was measured by using MTT assay Briefly, cells were seeded ... concentration/106 DCs LPS was used as a positive control for the production of NO Results are representative of three experiments LPS, as a positive control, produced significant amount of NO under same...
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Báo cáo y học: "Melanocortin peptides inhibit urate crystal-induced activation of phagocytic cells" ppsx
... cells through recognition of specific melanocortin receptors (MCRs to 5) Its anti-inflammatory action depends primarily on inhibition of cytokine production by target cells This is achieved by ... and release of active IL-1β [13] In this model, the second phase of the inflammatory response is driven by the IL-1β/ IL-1R pathway with activation of an MyD88-dependent signal- Page of (page number ... NF-κB activation followed by production of inflammatory mediators that elicit neutrophil recruitment into the joints, characteristic of the acute gouty inflammation [14] The importance of Mos...
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Báo cáo y học: "Anti-class a scavenger receptor autoantibodies from systemic lupus erythematosus patients impair phagocytic clearance of apoptotic cells by macrophages in vitro" doc
... cells were CFSE+CD3+ Percentages of positive cells were analyzed by using the CellQuest software The inhibition effect of IgG on macrophage binding or ingestion of apoptotic cell was defined as ... http://arthritis-research.com/content/13/1/R9 Page of Figure THP-1 derived macrophage phagocytosis of apoptotic Jurkat cells A Apoptosis induction was confirmed by the detection of percentage of Annexin V and 7-AAD staining by flow cytometric ... systemic lupus erythematosus A Levels of anti-SRCR IgG for patients B Levels of anti-SR-A IgG for patients Inhibition of macrophage uptake of apoptotic cells by anti-class A scavenger receptors...
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Báo cáo y học: " Interaction between human lung fibroblasts and T-lymphocytes prevents activation of CD4+ cells" pdf
... concentration of × 106 cells in ml of supplemented RPMI in the absence or presence of µg/ml of ionomycin and 10 ng/ml of PMA, plates were then incubated in a humidified atmosphere of 5% CO2 at ... cell-induced fibroblast activation is likely mediated by soluble factors produced by lymphocytes The increased expression of COX-2 induced by T cells on fibroblasts is of great interest considering ... persistent and excessive condition of immune activation of these cells may be important as well In view of the above findings, that fibroblasts are capable of interacting with T-lymphocytes,...
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Báo cáo y học: "Circumvention of multi-drug resistance of cancer cells by Chinese herbal medicines" potx
... supported by a direct grant (2008.1.079) from The Chinese University of Hong Kong Page of Author details School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, ... 9(4):376-378 87 Tang T, Meng LH, Chen LJ, Ding J: Reversal of multidrug resistance and inhibition of DNA topoisomerase II by emulsion of seed oil of Brucea Javanica Chin Pharmacol Bull 2001, 17(5):539-542 ... carcinoma cells Cancer Chemother Pharmacol 2002, 49(4):261-266 doi:10.1186/1749-8546-5-26 Cite this article as: Chai et al.: Circumvention of multi-drug resistance of cancer cells by Chinese...
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Photoimmunotherapy of melanoma via combination of hypericin photodynamic therapy and in vivo stimulation of dendritic cells by PNGVL3 HFLEX plasmid DNA
... findings of Korbelik and Dougherty, 1999 whereby depletion of CD8+ T cells substantially impaired the ability of PDT to suppress the long-term growth of EMT6 as opposed to the depletion of CD4+ T cells ... modality of treatment is the main focus of the present study and will be discussed in detail later Intra-tumoral injection of DCs offers the theoretical advantage of in vivo loading and activation of ... photomicrogram Control: normal B16 tumor cells ………………… 50 Fig Electron photomicrogram B16 cells demonstrating features of necrosis after PDT……………………………………………………………………………… 51 Fig Electron photomicrogram...
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Báo cáo khoa học: "Intravenous colistin in the treatment of sepsis from multiresistant Gram-negative bacilli in critically ill patient" ppsx
... we report years of experience with intravenous colistin in the treatment of Gram- negative sepsis Patients and method We studied critically ill patients with sepsis caused by Gramnegative bacilli ... antibiotic of the polymyxin family that is rapidly bactericidal to Gram- negative bacteria The action of colistin is by a detergent-like mechanism, interfering with the structure and function of the ... prevalence of multiresistant Gram- negative strains in ICUs has recently rekindled interest in colistin [8–12], a bactericidal antibiotic that was used in the 1960s for treatment of infections caused by...
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Báo cáo khoa học: Activation of activating transcription factor 2 by p38 MAP kinase during apoptosis induced by human amylin in cultured pancreatic b-cells ppt
... demonstrate that suppression of p38 kinase activation by SB203580, as well as suppression of JNK1 activation by JNK inhibitor I and AS-jnk1, caused equal inhibition of c-Jun phosphorylation ATF-2 ... dose-responsive effect of hA on activation of p38 kinase In parallel experiments, we studied the effects of inhibition of ERK1 ⁄ and p38 kinase on hA-induced caspase-3 activation and apoptosis ... activation in islet b -cells, through activation of the JNK pathway [15,23] In the current study, we planned to determine whether either of the other two MAP kinases, ERK and p38 kinase, and activation...
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báo cáo hóa học:" Activation of human B cells by the agonist CD40 antibody CP-870,893 and augmentation with simultaneous toll-like receptor 9 stimulation" doc
... naïve B cells as defined by the presence or absence of CD27 expression We studied the effect of CP-870,893 on B cell activation and B cell stimulation of T cells, and we analyzed the effects of co-stimulating ... CP-870,893 on T cell stimulatory capacity of B cells Effect of3 Effect of CP-870,893 on T cell stimulatory capacity of B cells Purified CD19+ B cells from each of donors were stimulated as described ... CD19+ B cells and cultured in the presence of varying concentrations of either CP-870,893 or negative control hIgG2 Cells were analyzed by flow cytometry for viability and expression of cell...
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báo cáo hóa học: " Aspirin-triggered lipoxin A4 attenuates LPSinduced pro-inflammatory responses by inhibiting activation of NF-B and MAPKs in BV-2 microglial cells" pptx
... inhibition of infiammation by ATL is regulated by the MAPK pathway, we examined the effects of ATL on LPS- induced phosphorylation of ERK, p38 MAPK and JNK in BV-2 microglia by western blot analysis Cells ... Inhibition of the nuclear accumulation of the NF-B p65 subunit and degradation of IB-a by ATL in LPS- stimulated BV-2 microglial cells (A) BV-2 cells were stimulated with 100 ng/ml LPS for the ... http://www.jneuroinflammation.com/content/8/1/95 Page of 12 Figure Inhibition of LPS- induced phosphorylation of ERK and p38 MAPK in BV-2 microglial cells BV-2 cells were stimulated with 100 ng/ml LPS in the absence or presence of 100 nM...
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báo cáo hóa học: " Anandamide inhibits Theiler’s virus induced VCAM-1 in brain endothelial cells and reduces leukocyte transmigration in a model of blood brain barrier by activation of CB1 receptors" pdf
... on b End5 cells and increased by TMEV infection [24] We first analyzed the effect of AEA on the production of VCAM-1 by TMEV-infected brain endothelial cells Dose response studies of AEA on sVCAM-1 ... TMEV may enter the CNS by infection of cerebrovascular endothelial cells Thus, infection of endothelial cells might represent one of the first events in the pathogenesis of TMEV-induced demyelination ... of the endothelium [41,19] In our study, VCAM-1 suppression by AEA in brain endothelial cells was mainly mediated by the activation of CB1 receptors Most importantly, AEA-induced inhibition of...
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báo cáo hóa học:" Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ ADAM17 gene expression during in vitro maturation of porcine COCs" doc
... lower level of these gene expressions resulting from the addition of U0126 was not overcome by the addition of EGF, the addition of EGF to H89- or SB203580-containning medium overcame the negative ... U0126 for 40 h Although the negative effects of H89 and SB20580 were overcome by the addition of EGF, the treatment with EGF did not overcome the negative effects of U0126 Discussion Recently, ... granulosa cells, it has been reported that the phosphorylation of CREB was induced by FSH within 1.5 h [29] Our recent study showed in rat granulosa cells that phosphorylation of CREB was induced by...
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Báo cáo y học: "Selective killing of human immunodeficiency virus infected cells by non-nucleoside reverse transcriptase inhibitor-induced activation of HIV proteas" doc
... of chronically infected cells, MT4-LTR-EGFP-IIIB cells as well as MT4-LTR-EGFP parental cells were treated with μM VRX-480773 for days Quantification of live cells by microscopic evaluation of ... effects of NNRTIs on virus producing cells regardless of their effect on Page of 14 reverse transcription, since the proportion of virus producing cells in this system does not depend on infection of ... accelerating the death of virus producing cells on the other The data presented here provide proof of concept for a drug induced killing of HIV producing cells, but more potent inducers of Gag-Pol dimerization...
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Tài liệu Báo cáo khoa học: Efficient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf
... possibility of a saturable mechanism of entry (Fig 1A); identical results were obtained with a control ODN (not shown) Examination of the cells by light microscopy showed that untreated cells, cells ... activity of STAT3 and colocalizes with STAT3 to the cytoplasm of SW480 cells The transcriptional activity of STAT3 after treatment of the cells with hpST3dODN was analysed in SW480 cells transfected ... after 72 h, the amount of dead cells and debris made it difficult to count dead cells Analysis by flow cytometry clearly showed the cells that had incorporated hpST3dODN (FITC positive) were those...
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Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf
... 81 lg of protein was added to each well The amount of S100A4 in the II-11b cells was 1.2% of that in the pHb-1 cells after exposure of the film and 12% after exposure (Fig 2A) The level of S100A4 ... Expression of S100A4 in pHb-1 and II-11b cells (A) Determination of S100A4 by western blotting of cell lysates from pHb-1 and II-11b cells attached to plastic, using a total protein concentration of ... Fig The expression of MT1-MMP and TIMP-2 in pHb-1 and II11b cells Determination of MT1-MMP (A) and TIMP-2 (B) by western blot of cell lysates from pHb-1 and II-11b cells (A) Cells were attached...
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